Pseudomonas putida is a gram-negative aerobe rarely involved in human infections and considered, as opposed to Pseudomonas aeruginosa, to be a low-grade pathogen (22). Resistance to carbapenems in P. putida may be due to metallo-β-lactamases (MBLs) such as IMP-1, IMP-12, VIM-1, VIM-2, and VIM-6 (4-7, 9, 14, 16). These MBL-encoding genes are part of gene cassettes located in class 1 integron structures (21). The blaVIM-2 gene was first identified from a P. aeruginosa isolate recovered in 1996 from Marseilles, France (13). Further studies showed that a single blaVIM-2-positive isolate had disseminated in the hematology unit where it had initially been identified (1). The blaVIM-2 gene cassette was always identified throughout the world as part of class 1 integrons varying in size and structure and is now the most prevalent carbapenemase gene (10, 12, 21). The aim of our study was to analyze the β-lactamase content of a carbapenem-resistant P. putida strain that was isolated from the same hospitalization unit as that mentioned above and that had carbapenemase activity. P. putida strain 9335 was isolated in January 2004 from bronchial aspirate and several blood cultures of a 59-year-old immunocompromised patient treated with an imipenem-containing regimen for 5 days. P. putida 9335 was identified using the API 32GN system (bioMerieux, Marcy-L'Etoile, France) and confirmed by 16S rRNA gene sequencing. P. putida 9335 was resistant to all β-lactams, including meropenem and imipenem (Table (Table1).1). It was also resistant to aminoglycosides, fluoroquinolones, sulfonamides, and chloramphenicol and remained susceptible only to colistin and rifampin. TABLE 1. MICs of β-lactams for P. putida 9335, P. putida reference strain CIP104063, E. coli DH10B harboring recombinant plasmid p9335H, and E. coli reference strain DH10B Production of an MBL was revealed by using Etest strips with imipenem and EDTA (AB Biodisk, Solna, Sweden) (20). Whole-cell DNA of P. putida 9335 was extracted as described previously (11) and used as a template in PCR, followed by cloning experiments. Cloning of the blaVIM-2 gene into Escherichia coli DH10B was performed by using HindIII-restricted genomic DNA of P. putida 9335 that was subsequently ligated into HindIII-restricted pBK-CMV phagemid (Stratagene, Amsterdam, The Netherlands), and the recombinant plasmid p9335H expressing VIM-2 (Table (Table1)1) was selected as described previously (11). The nucleotide sequence of the ca. 14-kb insert of plasmid p9335H was determined. It contained an 11,172-bp-long transposon termed Tn1332, closely related to Tn1331 and belonging to the Tn3 family, that had been identified previously in a Klebsiella pneumoniae isolate from Argentina (17). Tn1331 is closely related to Tn3, with an additional 3-kb fragment containing several antibiotic resistance genes, namely, aacA4, aadA1, and blaOXA-9, but does not possess any attI1 site (Fig. (Fig.1)1) (3). As reported for Tn1331, the aadA1 and blaOXA-9 gene cassettes are fused as a single gene cassette that may have arisen as a consequence of a recombination event involving two integrons (15). The first five amino acids of the leader peptide of β-lactamase TEM-1 were fused to the AAC(6′)-Ib protein (2), with this fusion likely being the consequence of a 520-bp duplication, including part of tnpR and the 5′ part of the blaTEM-1 gene, during the genesis of Tn1331 (15, 17, 18). Compared to Tn1331, Tn1332 carried the blaVIM-2 gene cassette that had been inserted between the aacA4 and aadA1 aminoglycoside resistance gene cassettes (Fig. (Fig.1).1). In addition, Tn1332 carried two novel insertion sequences, ISPpu17 and ISPpu18 (Fig. (Fig.1).1). ISPpu17, an IS30 family member, is 1,066 bp long, and its transposase (321 amino acids) shares 44% identity with that of ISPlu1 from Photorhabdus luminescens. The inverted repeats (IRs) of ISPpu17 are 22 bp long, and transposition of ISPpu17 generated a 3-bp duplication at its insertion site. ISPpu18, a member of the IS4 family, is 1,192 bp long and encodes a 326-amino-acid transposase which shares 94% protein identity with ISPre2 from Pseudomonas resinovorans and with IS1384 located on a plasmid from P. putida. The IRs of ISPpu18 are 12 bp long, and transposition of ISPpu18 generated a 4-bp duplication. FIG. 1. Structure comparison of Tn3 (a), Tn1331 (20) (b), and Tn1332 (c). The arrows indicate the locations of the genes (tnpA, tnpR, aacA4, blaVIM-2, aadA1, blaOXA-9, and blaTEM-1). The white circles indicate 59-be associated with genes (aacA4, blaVIM-2, and ... Immediately upstream and downstream of the 38-bp-long IRs of Tn1332, a 5-bp duplication was identified that was the signature of the transposition process for blaVIM-2 acquisition. The common promoter sequences present in the 5′ conserved region of class 1 integrons and responsible for the expression of gene cassettes were absent (8). Thus, the promoter sequences enhancing blaVIM-2 expression in Tn1332 might be the same as those described for Tn1331 that enhanced blaTEM-1 gene expression, being part of the 520-bp direct repeats upstream of the aacA4 gene (19). However, the presence of ISPpu17 in Tn1332 might also be the source of additional promoter sequences involved in expression of the blaVIM-2 gene (Fig. (Fig.11). Analytical isoelectric focusing was performed with β-lactamase extracts of cultures of P. putida 9335 and E. coli DH10B(p9335H), as described previously (11). Three identical pI values were visualized from both extracts that confirmed that all β-lactamase genes were expressed. Two bands were detected at pI values of 5.4 and 6.9, consistent with the expression of β-lactamases TEM-1 and OXA-9 (2), respectively, whereas a pI value of 5.6 corresponded to expression of VIM-2 (13; data not shown). A pulsed-field gel electrophoresis analysis was performed as described previously (1), followed by an I-CeuI digestion and a Southern blot hybridization (13) analysis for determination of the precise genetic location of the blaVIM-2 gene in P. putida 9335. Chromosomal DNAs from VIM-2-positive P. aeruginosa isolate COL-1 (13) and from the P. putida CIP104063 reference strain were included. The membrane was successively hybridized with probes for blaVIM-2 and 16S and 23S rRNA genes, as described previously (13). A strong hybridization signal was detected with the blaVIM-2-specific probe that did not cohybridize with the RNA-specific probe (Fig. (Fig.2).2). Plasmid content of the P. putida 9335 isolate identified a ca. 30-kb plasmid by a positive signal with the blaVIM-2 probe after Southern blot hybridization (Fig. (Fig.2).2). However, attempts to transfer the β-lactam resistance marker into E. coli DH10B and P. aeruginosa PU21 by electroporation failed (13). FIG. 2. (A) Pulsed-field gel electrophoresis profiles of I-CeuI digestion of whole-cell DNAs of Pseudomonas strains. Lane M, molecular size marker (band sizes are in kilobase pairs); lane 1, P. aeruginosa COL-1 (blaVIM-2 positive) (13); lane 2, P. putida reference ... We report here another transposon structure likely at the origin of blaVIM-2 acquisition that did not directly involve an integron structure. Surprisingly, whereas transfer of an identical blaVIM-2-carrying genetic structure from P. aeruginosa to P. putida could have been expected, it was not the case here. These findings underline the important genetic plasticity at the origin of acquisition and dissemination of MBL genes.