1. Network pharmacology study reveals energy metabolism and apoptosis pathways-mediated cardioprotective effects of Shenqi Fuzheng
- Author
-
Liangfeng Liu, Xin Shao, Jie Liao, Ni Ai, Xiaohui Fan, Cui Hao, Yangang Song, and Wenhua Huang
- Subjects
Male ,0301 basic medicine ,Cardiotonic Agents ,Gene regulatory network ,Peroxisome proliferator-activated receptor ,Apoptosis ,Myocardial Reperfusion Injury ,Traditional Chinese medicine ,Pharmacology ,Cell Line ,Rats, Sprague-Dawley ,Transcriptome ,Mice ,03 medical and health sciences ,Western blot ,Drug Discovery ,Animals ,Medicine ,Ventricular remodeling ,Codonopsis ,chemistry.chemical_classification ,biology ,medicine.diagnostic_test ,business.industry ,Cell Adhesion Inhibition ,medicine.disease ,biology.organism_classification ,RAW 264.7 Cells ,030104 developmental biology ,Gene Expression Regulation ,chemistry ,Energy Metabolism ,business ,Drugs, Chinese Herbal ,Signal Transduction - Abstract
Ethnopharmacological relevance Shenqi Fuzheng (SQ) is a renowned traditional Chinese medicine extracted from Radix Codonopsis and Radix Astragali. Although SQ is widely used to treat myocardial ischemia-reperfusion (I/R) injury, the molecular mechanisms supporting its clinical application remain elusive. Aim of the study The purpose of current study was to understand its cardioprotective effects at the molecular level using network pharmacology approach. Materials and method In an I/R injury animal model, the beneficial pharmacological activities of SQ were confirmed by decreased infarct range observed on drug treated rats versus control group. Additionally, several serum biochemical indicators were in concord with this observation. Subsequently, a microarray experiment was performed to reveal the influence on injured heart at the gene expression level by this TCM injection. We then proposed a network analysis algorithm NTRA to discover the key nodes based on both disease network structure and transcriptomics. Using NRIODN, a method developed by our group previously, the holistic changes on the gene network induced by for I/R injury and SQ treatment were evaluated. Results Pathway enrichment analysis of highly ranked genes by NTRA showed that PPAR and apoptosis pathways were highly related to I/R injury. Finally, western blot results showed increased level of the PPARα and BAX protein in the heart after injection treatment which confirmed the hypothesis. Conclusion In conclusion, our results suggest that SQ injection exerts protective effect against myocardial ischemia-reperfusion injury through multiple pathways, including myocardial energy metabolism improvement, cell adhesion inhibition, inflammatory reaction perturbation, myocardial apoptosis reduction and ventricular remodeling avoidance.
- Published
- 2018