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1. A quantitative analysis of gilthead seabream (Sparus aurata) juvenile dentition as a tool to assess the effect of diet

Author: Paul Witten, M.A.G. Owen, Luca Parma, Ann Huysseune, Serena Busti, Alessio Bonaldo, A.M. de Azevedo, Ramon Fontanillas, de Azevedo A.M., Fontanillas R., Owen M.A.G., Busti S., Parma L., Bonaldo A., Witten P.E., and Huysseune A.
Subjects: 0106 biological sciences, Gilthead Seabream, Teeth, Sparidae, Zoology, 010603 evolutionary biology, 01 natural sciences, Perciformes, 03 medical and health sciences, Mediterranean sea, Aquaculture, Sparus aurata, Dentition, Juvenile, Gilthead seabream, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, 0303 health sciences, biology, Lower jaw, business.industry, biology.organism_classification, Dentary, Pellet size, Animal Science and Zoology, business
Abstract: Gilthead seabream (Sparus aurata Linnaeus, 1758 (Perciformes, Sparidae)) is an important aquaculture species in the Mediterranean Sea basin. Yet, quantitative data on its dentition under standard farming conditions are currently lacking. Furthermore, it is unknown if the dentition can adapt to food of different sizes. Here, we describe the lower jaw dentition of juvenile S. aurata fed a standard pellet size (4 mm) and present a detailed analysis of 11 representative teeth. Overall, the number of teeth showed large individual variation, but it was not significantly related to fish length. Considerable left–right differences were observed, without clear side dominance. We also assessed the influence of feeding S. aurata a smaller (2 mm) or larger (6 mm) pellet size. Four months of feeding with different pellet sizes did not cause detectable differences in total tooth number on the dentaries at the time of harvest, nor in size of the teeth assumed to be most relevant in food processing. If and how different pellet sizes may nevertheless affect digestion, and eventually fish health, is subject for further studies.
Published: 2021
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2. Effects of increasing dietary level of organic acids and nature-identical compounds on growth, intestinal cytokine gene expression and gut microbiota of rainbow trout (Oncorhynchus mykiss) reared at normal and high temperature

Author: Federica D’Amico, Pier Paolo Gatta, Marco Candela, Barbara Rossi, Ester Grilli, Daniel Scicchitano, Luca Parma, Enrico Volpe, Sara Ciulli, Andrea Piva, Alessio Bonaldo, Nicole Francesca Pelusio, Pelusio N.F., Rossi B., Parma L., Volpe E., Ciulli S., Piva A., D'Amico F., Scicchitano D., Candela M., Gatta P.P., Bonaldo A., and Grilli E.
Subjects: 0301 basic medicine, Hot Temperature, Time Factors, Gene Expression, Growth, Aquatic Science, Gut flora, Bacterial Physiological Phenomena, Feed conversion ratio, Citric Acid, Eating, 03 medical and health sciences, chemistry.chemical_compound, Intestinal mucosa, Gene expression, Animals, Environmental Chemistry, Food science, chemistry.chemical_classification, Gut microbiome, High rearing temperature, biology, 04 agricultural and veterinary sciences, General Medicine, Nature-identical compound, biology.organism_classification, Animal Feed, Sorbic Acid, Thymol, Cytokine gene expression, Diet, Gastrointestinal Microbiome, Lactic acid, Intestines, Rainbow trout, 030104 developmental biology, chemistry, Benzaldehydes, Oncorhynchus mykiss, 040102 fisheries, Cytokines, 0401 agriculture, forestry, and fisheries, Feed utilization, Organic acid, Bacteria
Abstract: Organic acids (OA) and nature-identical compounds (NIC) such as monoterpenes and aldehydes are well-known growth and health promoters in terrestrial livestock while their application for fish production is recent and their mechanisms of action require further study. Hence, this study tested the increasing dietary level (D0, D250, D500, D1000; 0, 250, 500 and 1000 mg kg feed−1 respectively) of a microencapsulated blend containing citric and sorbic acid, thymol and vanillin over 82 days on rainbow trout to assess the effects on growth, feed utilization, intestine cytokine gene expression and gut microbiota (GM). Furthermore, the effects on intestinal cytokine gene expression and GM were also explored after one week at high water temperature (23 °C). OA and NIC improved specific growth rate (SGR) and feed conversion rate (FCR) during the second half (day 40–82) of the feeding trial, while at the end of the trial protein (PER) and lipid efficiency (LER) increased with increasing dietary level. GM diversity and composition and cytokine gene expression analysis showed no significant differences in fish fed with increasing doses of OA and NIC (82 days) demonstrating the absence of inflammatory activity in the intestinal mucosa. Although there were no statistical differences, GM structure showed a tendency in clustering D0 group separately from the other dietary groups and a trend towards reduction of Streptococcus spp. was observed in the D250 and D1000 groups. After exposure to high water temperature, lower GM diversity and increased gene expression of inflammatory intestinal cytokines were observed for both inclusions (D0 vs. D1000) compared to groups in standard condition. However, the gene up-regulation involved a limited number of cytokines showing the absence of a substantial inflammation process able to compromise the functional activity of the intestine. Despite further study should be conducted to fully clarify this mechanism, cytokines up-regulation seems to be concomitant to the reduction of the GM diversity and, particularly, to the reduction of specific lactic acid bacteria such as Leuconostoc. The application of the microencapsulate blend tested can be a useful strategy to improve growth and feed utilization in rainbow trout under normal temperature conditions. According to the results organic acids and nature-identical compounds did not revert the effects triggered by the increased temperature of water.
Published: 2020
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3. Evaluation of reproductive performances of the common octopus (Octopus vulgaris) reared in water recirculation systems and fed different diets

Author: Alessandra Roncarati, Oliviero Mordenti, Niccolò Guercilena, Alessio Bonaldo, Pietro Emmanuele, Luca Parma, Antonio Casalini, Casalini A., Roncarati A., Emmanuele P., Guercilena N., Bonaldo A., Parma L., and Mordenti O.
Subjects: Male, 0301 basic medicine, Eggs, Octopodiformes, Octopus vulgaris, RAS tank, broodstock diet, reproductive performance, lcsh:Medicine, Aquaculture, Article, 03 medical and health sciences, Octopus, Animal science, biology.animal, Animals, lcsh:Science, Incubation, Multidisciplinary, biology, Hatching, Reproduction, Biological techniques, lcsh:R, Common octopus, Water, Recirculating aquaculture system, 04 agricultural and veterinary sciences, biology.organism_classification, Animal Feed, Diet, 030104 developmental biology, Seafood, 040102 fisheries, 0401 agriculture, forestry, and fisheries, %22">Fish , Female, lcsh:Q, Zoology
Abstract: The reproductive performance of Octopus vulgaris broodstocks fed two different diets (mixed fish [F group, BW 1,048.14 g] or mixed crustaceans [C group, BW 998.44 g]) was analyzed using an experimental recirculating aquaculture system consisting of a tank equipped with spawning and incubation chambers. A total of 8 females (F1–4; C1–4), and 8 males (M1–M8) were selected. DI of the C group females was significantly (p
Published: 2020
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4. Colletotrichum spp: SENSIBILIDADE À FUNGICIDAS E REAÇÃO À CULTIVARES DE SOJA

Author: Solange Maria Bonaldo, Wesley Dantas de Souza, Sylvia Raquel Gomes Moraes, and Jaqueline Bezerra da Silva
Subjects: biology, Carbendazim, Inoculation, biology.organism_classification, Conidium, Fungicide, chemistry.chemical_compound, Horticulture, chemistry, Colletotrichum, Azoxystrobin, Glycine max, tombamento, incidência, severidade, antracnose, Epoxiconazole, Cultivar
Abstract: Antracnose é uma das doenças economicamente mais importantes da soja e de difícil controle no Mato Grosso, causando perdas significativas de produtividade. Objetivo foi verificar a sensibilidade de fungicidas e reação de cultivares de soja a 30 isolados de Colletotrichum. A ação de sete fungicidas (azoxistrobina + ciproconazol, piraclostrobina + epoxiconazol, trifloxistrobina + protioconazol, piraclostrobina, carbendazim, fluxpiroxada + piraclostrobina e azoxistrobina) foi avaliada medindo o crescimento micelial in vitro. Todos os fungicidas afetaram o crescimento micelial, mas piraclostrobina + epoxiconazol e piraclostrobina foram os mais eficientes. Além disso, 6 cultivares foram inoculadas com 27 isolados para análise de tombamento pré e pós-emergência, incidência e severidade da doença. Os dados de incidência e severidade foram submetidos à fórmula da Área Abaixo da Curva de Progresso da Doença. Interação significativa foi observada entre isolados e cultivares em todas as variáveis. Os isolados IT-4, IT-6, LU-3, MT-1, NU-5, PN-1, QU-2, SO-6, SO-11 e VR-1 apresentaram maior tombamento e AACPD. Exceto QU-2 todos os isolados apresentam conídios falcados. As cultivares 8866, 1179 e 9144 apresentaram menor incidência e severidade da doença. Palavras-chave: Glycine max; tombamento; incidência; severidade; antracnose. Colletotrichum spp.: Sensibility to fungicides and reaction of commercial germoplasm of soybean ABSTRACT: Anthracnose is one of the most economically important disease of soybean and difficult to control at Mato Grosso State, causing significant yield losses. Objective was to verify the fungicides sensitivity and soybean cultivars reaction to 30 isolates of Colletotrichum. The action of seven fungicides (azoxystrobin+cyproconazole, pyraclostrobin+epoxiconazole, trifloxystrobin+protioconazole, pyraclostrobin, carbendazim, fluxpyroxade+pyraclostrobin and azoxystrobin) was evaluated measuring in vitro mycelial growth. All fungicides affected the mycelial growth development, but pyraclostrobin + epoxiconazole and pyraclostrobin were the most efficient. Besides, 6 soybean cultivars were inoculated with 27 isolates to analyze pre and post-emergence damping-off, disease incidence and severity. Incidence and severity data were submitted to the formula Area Under the Disease Progress Curve. Significant interaction was observed between isolates and cultivars in all variables. Isolates IT-4, IT-6, LU-3, MT-1, NU-5, PN-1, QU-2, SO-6, SO-11 and VR-1 showed higher damping-off and AUDPC. Excepting the QU-2, all isolates have falcate conidia. Cultivars 8866, 1179 and 9144 presented the lowest disease severity and incidence. Keywords: Glycine max; damping-off; incidence; severity; anthracnose.
Published: 2021
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5. Programas de fungicidas no controle de antracnose na cultura da soja

Author: Luana Maria de Rossi Belufi, Ivan Vilela Andrade Fiorini, Solange Maria Bonaldo, Fábio Kempim Pittelkow, Cassiano Spaziani Pereira, and Lorrayne Ferreira Oliveira
Subjects: Fungicide, Propiconazole, chemistry.chemical_compound, Horticulture, chemistry, Chlorothalonil, biology, Azoxystrobin, Mancozeb, Epoxiconazole, Fluxapyroxad, Colletotrichum truncatum, biology.organism_classification
Abstract: Antracnose causada por espécies de Colletotrichum é uma das principais doenças da soja no Brasil. O objetivo foi avaliar programas de fungicidas no controle de antracnose e produtividade na cultura da soja, em experimento realizado na safra 2018/2019 em Lucas do Rio Verde – MT; utilizando cv. Monsoy 8210 IPRO com 20% de inoculação de sementes com Colletotrichum truncatum. O delineamento experimental foi blocos ao acaso com quatro repetições e aplicação de nove programas de fungicidas aos 30, 45 e 60 dias após a semeadura (DAS). Avaliou-se Área Abaixo da Curva de Progresso da Doença (AACPD), incidência de sintomas em vagens, número de vagens/plantas, desfolha, massa de mil grãos, produtividade e sanidade de grãos. A inoculação do patógeno reduziu o estande de plantas e número de plantas com cotilédones. Os programas de fungicidas reduziram incidência e severidade de antracnose na parte aérea das plantas em R5.3 e R5.5. O programa trifloxistrobina+ciproconazol (30 DAS) e trifloxistrobina+protioconazol+mancozebe (45 e 60 DAS) apresentou menor incidência de sintomas de antracnose em vagens. Os programas de fungicidas reduziram a desfolha no estádio fenológico R6. O programa piraclostrobina+epoxiconazol (30 DAS), fluxapiroxade+piraclostrobina +oxicloreto de cobre (45 DAS) e epoxiconazol+fluxapiroxade+ piraclostrobina+ oxicloreto de cobre (60 DAS); programa com trifloxistrobina+ciproconazol (30 DAS) e trifloxistrobina+protioconazol+mancozebe (45 e 60 DAS); e programa com propiconazol+difenoconazol (30 DAS), difeconazol+ciproconazol+clorotalonil (45 DAS) e azoxistrobina+benzovindiflupir+difeconazol+ciproconazol (60 DAS), reduziram a AACPD de antracnose. Maiores valores de produtividade e peso de mil sementes foram obtidos com o programa trifloxistrobina+ciproconazol (30 DAS) e trifloxistrobina+protioconazol+mancozebe (45 e 60 DAS).
Published: 2021
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6. In vitro antimicrobial activity of methanolic extracts from cutaneous secretions of Amazonian amphibians against phytopathogens of agricultural interest

Author: Stela Regina Ferrarini, Ana Gabriela Araújo Verçosa, Domingos de Jesus Rodrigues, Daiane Lopes de Oliveira, Solange Maria Bonaldo, and Livia Deice Raasch-Fernandes
Subjects: 0106 biological sciences, Science (General), conidia, Aspergillus flavus, 01 natural sciences, Conidium, Microbiology, Rhizoctonia solani, Q1-390, 03 medical and health sciences, conídios, Rhaebo guttatus, Mycelium, 030304 developmental biology, Rhinella marina, 0303 health sciences, biology, food and beverages, biology.organism_classification, inhibition, inibição, Spore, Macrophomina phaseolina, General Agricultural and Biological Sciences, Colletotrichum truncatum, Fusarium solani, 010606 plant biology & botany
Abstract: The biochemical defense mechanisms of amphibians involve cutaneous secretions of bioactive molecules with antimicrobial activity. This study evaluated the in vitro activity of methanolic extracts from cutaneous secretions of two amphibian species of the Bufonidae family, Rhaebo guttatus and Rhinella marina, in the control of the phytopathogens Fusarium udum, Fusarium solani, Colletotrichum truncatum, Aspergillus flavus, Rhizoctonia solani, Macrophomina phaseolina, and Calonectria pseudometrosideri. The R. guttatus extract decreased the mycelial growth of F. udum, F. solani, A. flavus, and M. phaseolina at some tested concentrations. The R. marina extract decreased the mycelial growth of C. truncatum at the concentration of 0.5 mg mL-¹, and inhibited the mycelial growth of A. flavus at concentrations of 0.1 and 0.5 mg mL-¹, which was similar to the inhibition by the positive control. The R. marina extract also decreased the microsclerotia production by R. solani at concentrations of 0.2 and 0.3 mg mL-¹. In addition, the extracts inhibited conidial sporulation and germination at varying degrees. The inhibition of appressoria formation in C. truncatum by the R. guttatus and R. marina extracts was 85-99% and 63-100%, respectively. Our results demonstrated that treatment with extracts from R. guttatus and R. marina cutaneous secretions showed antifungal activity against the studied phytopathogens. RESUMO Os mecanismos de defesa bioquímica dos anfíbios envolvem secreções cutâneas de moléculas bioativas com atividade antimicrobiana. Este estudo avaliou a atividade in vitro de extratos metanólicos da secreção cutânea de duas espécies de anfíbios da família Bufonidae, Rhaebo guttatus e Rhinella marina, no controle dos patógenos Fusarium udum, Fusarium solani, Colletotrichum truncatum, Aspergillus flavus, Rhizoctonia solani, Macrophomina phaseolina e Calonectria pseudometrosideri. O extrato de R. guttatus inibiu o crescimento micelial de F. udum, F. solani, A. flavus e M. phaseolina em algumas concentrações testadas. O extrato de R. marina inibiu o crescimento micelial de C. truncatum na concentração de 0,5 mg mL-¹, e inibiu o crescimento micelial de A. flavus nas concentrações de 0,1 e 0,5 mg mL-¹, que foi semelhante à inibição pelo controle positivo. O extrato de R. marina também diminuiu a produção de microescleródios de R. solani nas concentrações de 0,2 e 0,3 mg mL-¹. Além disso, os extratos inibiram a esporulação e germinação de conídios em graus variados. A inibição da formação de apressórios em C. truncatum pelos extratos de R. guttatus e R. marina foi de 85%-99% e 63%-100%, respectivamente. Nossos resultados demonstraram que o tratamento com extratos da secreção cutânea de R. guttatus e R. marina apresentou atividade antifúngica sobre os fitopatógenos estudados.
Published: 2021
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7. Effect of Essential Oils on the Oxyntopeptic Cells and Somatostatin and Ghrelin Immunoreactive Cells in the European Sea Bass (Dicentrarchus labrax) Gastric Mucosa

Author: Serena Busti, Paolo Clavenzani, Pier Paolo Gatta, Claudio Tagliavia, Nadia Bernardi, Maurizio Mazzoni, Luca Parma, Giulia Lattanzio, Alessio Bonaldo, Mazzoni M., Lattanzio G., Bonaldo A., Tagliavia C., Parma L., Busti S., Gatta P.P., Bernardi N., and Clavenzani P.
Subjects: medicine.medical_specialty, Na+K+-ATPase, Veterinary medicine, Enteroendocrine cell, Growth hormone receptor, somatostatin, +, Article, essential oil, Internal medicine, SF600-1100, medicine, Gastric mucosa, Na, Sea bass, Na+/K+-ATPase, oxyntopeptic cells, ghrelin, enteroendocrine cells, Oxyntopeptic cell, ATPase, General Veterinary, biology, Chemistry, biology.organism_classification, medicine.anatomical_structure, Somatostatin, Endocrinology, QL1-991, Animal Science and Zoology, Ghrelin, Dicentrarchus, Zoology
Abstract: Simple Summary Nutritional strategies focused on the use of botanicals as modulators of several physiological responses and health promoters of the gastrointestinal tract have attracted interest in animal production. Previous research indicates the positive results of using essential oils (EOs) as natural feed additives for several farmed animals. In the last decades, these nutritional alternatives have been evaluated and reported in fish production in order to increase fish growth and feed utilization and to promote animal welfare. Therefore, the present study was designed to compare the effects of feed EO supplementation in two different forms (natural and composed of active ingredients obtained by synthesis) on the gastric mucosa in European sea bass. EOs decrease oxyntopeptic cells and increase somatostatin and ghrelin enteroendocrine cells. In addition, we showed that Na+K+-ATPase was expressed in oxyntopeptic cells (OPs) in the same way as H+K+-ATPase (typical marker for mammalian parietal cells) and, for this reason, consider Na+K+-ATPase a valid marker for OPs. Abstract The current work was designed to assess the effect of feed supplemented with essential oils (EOs) on the histological features in sea bass’s gastric mucosa. Fish were fed three diets: control diet (CTR), HERBAL MIX® made with natural EOs (N-EOs), or HERBAL MIX® made with artificial EOs obtained by synthesis (S-EOs) during a 117-day feeding trial. Thereafter, the oxyntopeptic cells (OPs) and the ghrelin (GHR) and somatostatin (SOM) enteroendocrine cells (EECs) in the gastric mucosa were evaluated. The Na+K+-ATPase antibody was used to label OPs, while, for the EECs, anti-SOM and anti-GHR antibody were used. The highest density of OP immunoreactive (IR) area was in the CTR group (0.66 mm2 ± 0.1). The OP-IR area was reduced in the N-EO diet group (0.22 mm2 ± 1; CTR vs. N-EOs, p < 0.005), while in the S-EO diet group (0.39 mm2 ± 1) a trend was observed. We observed an increase of the number of SOM-IR cells in the N-EO diet (15.6 ± 4.2) compared to that in the CTR (11.8 ± 3.7) (N-EOs vs. CTR; p < 0.05), but not in the S-EOs diet. These observations will provide a basis to advance current knowledge on the anatomy and digestive physiology of this species in relation to pro-heath feeds.
Published: 2021

8. ZIKA Virus Neutralizing Antibody Kinetics in Antenatally Exposed Infants

Author: Zilton Vasconcelos, Claudia Raja Gabaglia, Luana Damasceno, Patrícia Brasil, Myrna C. Bonaldo, Thomas Jaenisch, James D. Cherry, Liege Maria Abreu de Carvalho, Boris Pastorino, Andrea Zin, Maria Elisabeth Lopes Moreira, Otávio de Melo Espíndola, Denise Cotrim da Cunha, Marcos Vinicius da Silva Pone, Karin Nielsen-Saines, Raquel de Vasconcellos Carvalhaes de Oliveira, Sheila Moura Pone, Xavier de Lamballerie, and Ieda Pereira Ribeiro
Subjects: Male, Pathogenesis and Host Response, 0301 basic medicine, medicine.medical_specialty, Antibodies, Viral, Polymerase Chain Reaction, Gastroenterology, 03 medical and health sciences, 0302 clinical medicine, Pregnancy, Internal medicine, Parenchyma, medicine, Humans, Immunology and Allergy, 030212 general & internal medicine, Pregnancy Complications, Infectious, Neutralizing antibody, Subclinical infection, biology, Zika Virus Infection, business.industry, Infant, Newborn, Infant, Zika Virus, Hypervascularity, Odds ratio, Antibodies, Neutralizing, Infectious Disease Transmission, Vertical, 3. Good health, Kinetics, 030104 developmental biology, Infectious Diseases, Immunoglobulin M, biology.protein, Biomarker (medicine), Female, business, Biomarkers, Kappa
Abstract: Background Zika virus (ZIKV) is associated with severe congenital abnormalities and laboratory diagnosis of antenatal infection is difficult. Here we evaluated ZIKV neutralizing antibody (nAb) kinetics in infants born to mothers with PCR-confirmed ZIKV infection during pregnancy. Methods Neonates (n = 98) had serum specimens tested repeatedly for ZIKV nAb over the first 2 years of life using virus neutralization test (VNT). ZIKV neonatal infection was confirmed by RT-PCR in blood or urine and/or presence of ZIKV IgM antibodies, and results were correlated with infant clinical features. Results Postnatal laboratory evidence of ZIKV vertical transmission was obtained for 60.2% of children, while 32.7% exhibited clinical abnormalities. Congenital abnormalities were found in 37.3% of children with confirmed ZIKV infection and 31.0% of children without confirmed infection (P = .734). All but 1 child displayed a physiologic decline in ZIKV nAb, reflecting maternal antibody decay, despite an early ZIKV-IgM response in one-third of infants. Conclusions Infants with antenatal ZIKV exposure do not develop ZIKV nAb despite an early IgM response. Therefore, ZIKV VNT in children is not useful for diagnosis of congenital infection. In light of these findings, it remains to be determined if children infected in utero are potentially susceptible to reinfection.
Published: 2021
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9. Effects of calcium carbonate inclusion in low fishmeal diets on growth, gastrointestinal pH, digestive enzyme activity and gut bacterial community of European sea bass (Dicentrarchus labrax L.) juveniles

Author: Alessio Bonaldo, Ramon Fontanillas, Carmen Navarro-Guillén, Francisco Javier Moyano, Matteo Soverini, Luca Parma, Pier Paolo Gatta, Federica D’Amico, Marco Candela, Manuel Yúfera, Parma L., Yufera M., Navarro-Guillen C., Moyano F.J., Soverini M., D'Amico F., Candela M., Fontanillas R., Gatta P.P., and Bonaldo A.
Subjects: Teleost, Replacement, Aquatic Science, Daily rhythms, Feed conversion ratio, European sea ba, Bream Sparus-Aurata, 03 medical and health sciences, Fish meal, Pepsin, Gut bacterial community, Feed buffering capacity, Lactobacillus, Food science, Amylase, European sea bass, Sea bass, Fishmeal replacement, Gastrointestinal pH, Soybean-meal, 030304 developmental biology, 0303 health sciences, Capacity, biology, Protein, Production performance, 04 agricultural and veterinary sciences, Digestive enzyme, biology.organism_classification, Oil, Vegetable meals, 040102 fisheries, biology.protein, 0401 agriculture, forestry, and fisheries, Dicentrarchus
Abstract: Fishmeal (FM) possesses one of the highest buffering capacities in comparison to most alternative vegetable aquafeed ingredients and its decreasing content in current formulations might affect the ideal gastrointestinal environment for digestive enzyme action and gut bacterial community of carnivorous fish species. A study was undertaken, therefore, to assess growth response, gastrointestinal pH, digestive enzyme activity and gut bacterial community of European sea bass (Dicentrarchus labrax L.) juveniles fed low FM diets (10% FM) with or without a feed buffering additive, calcium carbonate (FM10 + B and FM10, respectively) in comparison to a standard FM diet (20% FM, FM20). Three isonitrogenous and isolipidic extruded diets were fed to triplicate fish groups of 80 individuals (initial weight: 23 g) to overfeeding over 64 days. No significant differences due to low FM dietary levels were observed in final body weight, specific growth rate, feed intake, feed and protein efficiency. Low FM diet did not affect gastrointestinal pH in the stomach, anterior intestine, mid-intestine and hindgut at 0, 4, 8 and 12 hours post meal (hpm) while the inclusion of calcium carbonate in low FM diet seems to slightly increase the pH in the hindgut at 12 hpm. The absence of significant differences of pepsin, chymotrypsin, amylase and lipase activity suggests a comparable digestive efficiency among treatments although trypsin activity was slightly reduced in low FM diets after 4 hpm. Decreasing FM content seems to exert an effect on the overall gut bacterial community analysed by next-generation sequencing even if no significant effects on specific bacterial component were detected. The gut bacterial community in all the treatments was particularly rich in lactic acid bacteria such as Lactobacillus spp. which may provide important beneficial functions for the host and be associated with a healthy intestinal epithelium. According to the results, increasing the feed buffering capacity does not seem to improve digestive conditions while it is feasible to include 10% FM dietary level in practical formulation for European sea bass juveniles without negatively affecting growth, feed efficiency and digestive luminal conditions. info:eu-repo/semantics/publishedVersion
Published: 2019
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10. Farmed and wild common sole (Solea solea L.): Comparative assessment of morphometric parameters, processing yields, selected nutritional traits and sensory profile

Author: Luca Parma, Marina Silvi, Cinzia Viroli, Pier Paolo Gatta, Anna Badiani, Federica Farabegoli, Alessio Bonaldo, Erika Bonvini, Maurizio Pirini, Parma, Luca, Badiani, Anna, Bonaldo, Alessio, Viroli, Cinzia, Farabegoli, Federica, Silvi, Marina, Bonvini, Erika, Pirini, Maurizio, and Gatta, Pier Paolo
Subjects: Common sole, Sensory analysi, Umami, Aquatic Science, Biology, Sensory analysis, 03 medical and health sciences, Nutrient, Farmed and wild common sole, Aquaculture, Food science, 030304 developmental biology, chemistry.chemical_classification, Morphometric parameter, 0303 health sciences, business.industry, Soleá, Solea solea, Fatty acid, 04 agricultural and veterinary sciences, biology.organism_classification, chemistry, Fatty acid profile, Flesh quality, Chewiness, 040102 fisheries, 0401 agriculture, forestry, and fisheries, business
Abstract: Recent important developments in sole aquaculture have increased the availability of the farmed product on the market. The aim of this research was to characterize and compare for the first time in common sole (Solea solea) morphometric parameters, nutrients and sensory traits of cultured and wild specimens. Farmed sole, while maintaining the characteristics of a lean fish species (2% of lipid content), displayed a EPA+DHA fillet content more than twice as high as its wild counterpart. Sensory traits of “potato”, “boiled fish”, sweet, firmness, astringency, chewiness, were correlated to farmed origin while whiteness, briny, “octopus”, “crab”, salty, acid, bitter, umami, intensity and juiciness were linked to wild origin. Intensive farming conditions improved the nutritional value of common sole in terms of lipid content and fatty acid profile and led to sweeter sensory traits particularly associated with a terrestrial vegetable perception.
Published: 2019
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11. Novel marine cyanobacteria from the Atlantic coast of Brazil

Author: Lorene Armstrong, Ezequiane Machado da Silva, Diego Bonaldo Genuário, Marcelo Gomes Marçal Vieira Vaz, Marli Fátima Fiore, and Hosana Maria Debonsi
Subjects: Cyanobacteria, Ecology, Phylogenetics, General Engineering, bacteria, biochemical phenomena, metabolism, and nutrition, Biology, biology.organism_classification, Isolation (microbiology)
Abstract: Marine cyanobacteria have emerged as a source of promising bioactive compounds. The isolation of strains from tropical environments seems to be an important step in the search for molecules display...
Published: 2020
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12. Geographical distribution, diversity and pathogenicity of Colletotrichum associ-ated with soybean anthracnose in Brazil

Author: A. R. R. Gaspareto, S. R. G. Moraes, and S. M. Bonaldo
Subjects: biology, fungi, food and beverages, glycine max, lcsh:A, biology.organism_classification, Pathogenicity, Conidium, Horticulture, morphological and cultural traits, Colletotrichum, Germination, Proteinase activity, Colletotrichum species, lcsh:General Works, Incubation, Mycelium
Abstract: Eighty-five Colletotrichum isolates were obtained from soybean croplands in 34 municipalities of Mato Grosso state. Our objectives were characterize the isolates through mycelial growth, cultural and morphological analyses, as well as assessing pathogenicity. To evaluate cultural characteristics, the mycelial growth was daily measured, and the color of colonies was characterized after ten days incubation. The morphology, length, and width of 50 conidia per isolate were assessed. The pathogenicity of twenty-one isolates was evaluated on seed germination rate, incidence, and detection of extracellular enzymes. The cultural and morphology characteristics of Colletotrichum isolates were greatly variable. All isolates studied were able to reduce seed germination, produce sign and proteinase activity. These results suggest there are more than one Colletotrichum species associated with soybean anthracnose in Mato Grosso State.
Published: 2020

13. Zebrafish ambra1a and ambra1b Silencing Affect Heart Development

Author: Martina Chrisam, Luisa Dalla Valle, Nicola Facchinello, Francesco Cecconi, Tatjana Skobo, Giacomo Meneghetti, Francesca Nazio, Paolo Bonaldo, and Camilla Maria Fontana
Subjects: autophagy, Settore BIO/06, animal structures, Biology, 03 medical and health sciences, 0302 clinical medicine, Gene silencing, natural sciences, Gene, Zebrafish, 030304 developmental biology, 0303 health sciences, Ambra1, PP2A, heart development, zebrafish, Heart development, fungi, Autophagy, Protein phosphatase 2, BECN1, biology.organism_classification, Cell biology, Animal Science and Zoology, 030217 neurology & neurosurgery, Developmental Biology
Abstract: In zebrafish, two paralogous genes, activating molecule in beclin-1 (BECN1)–regulated autophagy ambra1a and ambra1b, both required for the autophagic process and during development, encode the prot...
Published: 2020
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14. Post-acute COVID-19 syndrome after reinfection and vaccine breakthrough by the SARS-CoV-2 Gamma variant in Brazil

Author: Ketiuce de Azevedo Zukeram, Chris Smith, Cláudio Tadeu Daniel-Ribeiro, Otávio de Melo Espíndola, James A. G. Whitworth, Isabella Campos Vargas de Moraes, Michele Fernanda Borges da Silva, Stephanie Lema Suarez Penetra, Mia Ferreira Araujo, Claudia Raja Gabaglia, Maria Ogrzewalska, Trevon L Fuller, Mayumi Duarte Wakimoto, Myrna C. Bonaldo, Renata Serrano Lopes, Anielle da Pina-Costa, Alex Pauvolid-Corrêa, Heloisa F P Santos, Ighor Arantes, Guilherme Amaral Calvet, Paola Cristina Resende, Larissa R Lira-Silva, Lusiele Guaraldo, Karin Nielsen-Saines, Ana Beatriz Machado Lima, Marilda M. Siqueira, and Patrícia Brasil
Subjects: Microbiology (medical), Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Case Report, Infectious and parasitic diseases, RC109-216, Passive, health personnel, Microbiology, COVID-19 breakthrough infection, reinfection, Vaccine Related, Post-Acute COVID-19 Syndrome, Rare Diseases, Biodefense, Medicine, Humans, Waning immunity, Lung, COVID-19 Serotherapy, Vaccines, SARS-CoV-2 variants, Heterologous vaccine, biology, business.industry, SARS-CoV-2, Prevention, Immune escape, COVID-19, Inactivated, General Medicine, Pneumonia, Serum samples, post-acute COVID-19 syndrome, Virology, Emerging Infectious Diseases, Infectious Diseases, Good Health and Well Being, whole-genome sequencing, Medical Microbiology, Inactivated vaccine, biology.protein, Public Health and Health Services, Immunization, Antibody, business, Infection, Brazil
Abstract: We describe a case of prolonged COVID-19 caused by the SARS-CoV-2 Gamma variant in a fully vaccinated healthcare worker, 387 days after an infection caused by lineage B.1.1.33. Infections were confirmed by whole-genome sequencing and corroborated by the detection of neutralizing antibodies in convalescent serum samples. Considering the permanent exposure of this healthcare worker to SARS-CoV-2, the waning immunity after the first infection, the low efficacy of the inactivated vaccine at preventing COVID-19, the immune escape of the Gamma variant (VOC), and the burden of post-COVID syndrome, this individual would have benefited from an additional dose of a heterologous vaccine.
Published: 2022

15. Interaction Between Dietary Lipid Level and Seasonal Temperature Changes in Gilthead Sea Bream Sparus aurata: Effects on Growth, Fat Deposition, Plasma Biochemistry, Digestive Enzyme Activity, and Gut Bacterial Community

Author: Francisco Javier Moyano, Daniel Scicchitano, Federica D’Amico, Marco Candela, Alessio Bonaldo, Francesco Dondi, Nicole Francesca Pelusio, Pier Paolo Gatta, Manuel Yúfera, Luca Parma, Elisa Brini, Neda Gilannejad, European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Pelusio N.F., Scicchitano D., Parma L., Dondi F., Brini E., D'Amico F., Candela M., Yufera M., Gilannejad N., Moyano F.J., Gatta P.P., and Bonaldo A.
Subjects: digestive enzyme, gilthead sea bream (Sparus aurata L.), growth, Science, Dietary lipid, Ocean Engineering, Growth, Gut microbiota, plasma biochemistry, Aquatic Science, Gut flora, QH1-199.5, Oceanography, Feed conversion ratio, 03 medical and health sciences, Animal science, Pepsin, Lactobacillus, feeding strategie, Lipase, feeding strategies, 030304 developmental biology, Water Science and Technology, 0303 health sciences, Global and Planetary Change, biology, gut microbiota, Chemistry, General. Including nature conservation, geographical distribution, 04 agricultural and veterinary sciences, Gilthead sea bream (Sparus aurata L.), gut health, Digestive enzyme, biology.organism_classification, 040102 fisheries, biology.protein, Feeding strategies, 0401 agriculture, forestry, and fisheries, Alkaline phosphatase, Gut health, Plasma biochemistry
Abstract: A 121-day feeding trial was undertaken to test the effects of two dietary lipid levels (16 and 21% L16, L21) in triplicated gilthead sea bream groups (initial weight: 67.5 g) reared at two different water temperatures (high, H 23°C and low, L 17°C) in the same recirculation system but exposed to a switch in temperature after 58 days. Fish kept at H were transferred to L (HL transition, autumn shift), and the fish kept at L were exposed to H (LH transition, summer shift), while continuing to receive the same diet to apparent satiation in each group. At the end of the trial, no significant diet effect on specific growth rate (SGR), feed intake (FI), and feed conversion rate (FCR) were detected in fish exposed to HL transition compared with those exposed to LH transition, while gross lipid efficiency (GLE) and lipid efficiency ratio (LER) were higher in L16. After temperature changes, L16 displayed higher SGR, FI, GLE, and LER, while mesenteric fat index was reduced. After temperature changes, the combined effects of low lipid diet and low temperature conditions resulted in higher pepsin activity, while trypsin, chymotrypsin, and lipase activities were generally higher at high lipid content. The combined effect of diet and temperature did not alter the metabolic plasma profile, except for the observed final higher aspartate aminotransferase (AST) and alkaline phosphatase (ALP) values when combining high dietary lipid (L21) and temperature changes. Different diets showed a significantly different gut microbiome layout, only at high temperature with L16 diet resulting in a higher load of Lactobacillus. On the contrary, no dietary impact on ecosystem diversity was observed, independently from the temperature. In addition, L16 diet in the HL transition favored an increase in Weissella and Bradyrhizobium genera in the gut microbiome, while in the final condition of LH transition, L21 diet favored a significant increase in Streptococcus and Bacillus. According to the results, the utilization of 16% dietary lipid levels in gilthead sea bream should be preferred during seasonal temperature changes in order to optimize feed utilization and gut health., This research was undertaken under the MedAID (Mediterranean Aquaculture Integrated Development) project, which has received funding from the European Union’s Horizon 2020 Research and Innovation Programme, Call H2020-SFS-23-2016, Grant agreement no. 727315 (http://www.medaid-h2020.eu/). MY and NG received support from the Spanish Ministry of Science, Innovation and Universities (MCIU), the State Research Agency (AEI), the European Regional Development Fund (FEDER/ERDF), and project Thermodigest (RTI2018-096134-B-I00) granted to MY.
Published: 2021
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16. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1

Author: Klionsky, Daniel, Abdel-Aziz, Amal Kamal, Abdelfatah, Sara, Abdellatif, Mahmoud, Abdoli, Asghar, Abel, Steffen, Abeliovich, Hagai, Abildgaard, Marie, Abudu, Yakubu Princely, Acevedo-Arozena, Abraham, Adamopoulos, Iannis, Adeli, Khosrow, Adolph, Timon, Adornetto, Annagrazia, Aflaki, Elma, Agam, Galila, Agarwal, Anupam, Aggarwal, Bharat, Agnello, Maria, Agostinis, Patrizia, Agrewala, Javed, Agrotis, Alexander, Aguilar, Patricia, Ahmad, S Tariq, Ahmed, Zubair, Ahumada-Castro, Ulises, Aits, Sonja, Aizawa, Shu, Akkoc, Yunus, Akoumianaki, Tonia, Akpinar, Hafize Aysin, Al-Abd, Ahmed, Al-Akra, Lina, Al-Gharaibeh, Abeer, Alaoui-Jamali, Moulay, Alberti, Simon, Alcocer-Gómez, Elísabet, Alessandri, Cristiano, Ali, Muhammad, Alim Al-Bari, M Abdul, Aliwaini, Saeb, Alizadeh, Javad, Almacellas, Eugènia, Almasan, Alexandru, Alonso, Alicia, Alonso, Guillermo, Altan-Bonnet, Nihal, Altieri, Dario, Álvarez, Élida, Alves, Sara, Alves Da Costa, Cristine, Alzaharna, Mazen, Amadio, Marialaura, Amantini, Consuelo, Amaral, Cristina, Ambrosio, Susanna, Amer, Amal, Ammanathan, Veena, An, Zhenyi, Andersen, Stig, Andrabi, Shaida, Andrade-Silva, Magaiver, Andres, Allen, Angelini, Sabrina, Ann, David, Anozie, Uche, Ansari, Mohammad, Antas, Pedro, Antebi, Adam, Antón, Zuriñe, Anwar, Tahira, Apetoh, Lionel, Apostolova, Nadezda, Araki, Toshiyuki, Araki, Yasuhiro, Arasaki, Kohei, Araújo, Wagner, Araya, Jun, Arden, Catherine, Arévalo, Maria-Angeles, Arguelles, Sandro, Arias, Esperanza, Arikkath, Jyothi, Arimoto, Hirokazu, Ariosa, Aileen, Armstrong-James, Darius, Arnauné-Pelloquin, Laetitia, Aroca, Angeles, Arroyo, Daniela, Arsov, Ivica, Artero, Rubén, Asaro, Dalia Maria Lucia, Aschner, Michael, Ashrafizadeh, Milad, Ashur-Fabian, Osnat, Atanasov, Atanas, Au, Alicia, Auberger, Patrick, Auner, Holger, Aurelian, Laure, Autelli, Riccardo, Avagliano, Laura, Ávalos, Yenniffer, Aveic, Sanja, Aveleira, Célia Alexandra, Avin-Wittenberg, Tamar, Aydin, Yucel, Ayton, Scott, Ayyadevara, Srinivas, Azzopardi, Maria, Baba, Misuzu, Backer, Jonathan, Backues, Steven, Bae, Dong-Hun, Bae, Ok-Nam, Bae, Soo Han, Baehrecke, Eric, Baek, Ahruem, Baek, Seung-Hoon, Baek, Sung Hee, Bagetta, Giacinto, Bagniewska-Zadworna, Agnieszka, Bai, Hua, Bai, Jie, Bai, Xiyuan, Bai, Yidong, Bairagi, Nandadulal, Baksi, Shounak, Balbi, Teresa, Baldari, Cosima, Balduini, Walter, Ballabio, Andrea, Ballester, Maria, Balazadeh, Salma, Balzan, Rena, Bandopadhyay, Rina, Banerjee, Sreeparna, Banerjee, Sulagna, Bánréti, Ágnes, Bao, Yan, Baptista, Mauricio, Baracca, Alessandra, Barbati, Cristiana, Bargiela, Ariadna, Barilà, Daniela, Barlow, Peter, Barmada, Sami, Barreiro, Esther, Barreto, George, Bartek, Jiri, Bartel, Bonnie, Bartolome, Alberto, Barve, Gaurav, Basagoudanavar, Suresh, Bassham, Diane, Bast, Robert, Basu, Alakananda, Batoko, Henri, Batten, Isabella, Baulieu, Etienne, Baumgarner, Bradley, Bayry, Jagadeesh, Beale, Rupert, Beau, Isabelle, Beaumatin, Florian, Bechara, Luiz, Beck, George, Beers, Michael, Begun, Jakob, Behrends, Christian, Behrens, Georg, Bei, Roberto, Bejarano, Eloy, Bel, Shai, Behl, Christian, Belaid, Amine, Belgareh-Touzé, Naïma, Bellarosa, Cristina, Belleudi, Francesca, Belló Pérez, Melissa, Bello-Morales, Raquel, Beltran, Jackeline Soares de Oliveira, Beltran, Sebastián, Benbrook, Doris Mangiaracina, Bendorius, Mykolas, Benitez, Bruno, Benito-Cuesta, Irene, Bensalem, Julien, Berchtold, Martin, Berezowska, Sabina, Bergamaschi, Daniele, Bergami, Matteo, Bergmann, Andreas, Berliocchi, Laura, Berlioz-Torrent, Clarisse, Bernard, Amélie, Berthoux, Lionel, Besirli, Cagri, Besteiro, Sebastien, Betin, Virginie, Beyaert, Rudi, Bezbradica, Jelena, Bhaskar, Kiran, Bhatia-Kissova, Ingrid, Bhattacharya, Resham, Bhattacharya, Sujoy, Bhattacharyya, Shalmoli, Bhuiyan, Md Shenuarin, Bhutia, Sujit Kumar, Bi, Lanrong, Bi, Xiaolin, Biden, Trevor, Bijian, Krikor, Billes, Viktor, Binart, Nadine, Bincoletto, Claudia, Birgisdottir, Asa, Bjorkoy, Geir, Blanco, Gonzalo, Blas-Garcia, Ana, 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Anna, Hernández, Agustín, Hernandez, Carlos, Hernandez-Diaz, Sergio, Hernandez-Gea, Virginia, Herpin, Amaury, Herreros, Judit, Hervás, Javier, Hesselson, Daniel, Hetz, Claudio, Heussler, Volker, Higuchi, Yujiro, Hilfiker, Sabine, Hill, Joseph, Hlavacek, William, Ho, Emmanuel, Ho, Idy, Ho, Philip Wing-Lok, Ho, Shu-Leong, Ho, Wan Yun, Hobbs, G Aaron, Hochstrasser, Mark, Hoet, Peter, Hofius, Daniel, Hofman, Paul, Höhn, Annika, Holmberg, Carina, Hombrebueno, Jose, Yi-Ren Hong, Chang-Won Hong, Hooper, Lora, Hoppe, Thorsten, Horos, Rastislav, Hoshida, Yujin, Hsin, I-Lun, Hsu, Hsin-Yun, Hu, Bing, Hu, Dong, Hu, Li-Fang, Hu, Ming Chang, Hu, Ronggui, Hu, Wei, Hu, Yu-Chen, Hu, Zhuo-Wei, Hua, Fang, Hua, Jinlian, Hua, Yingqi, Huan, Chongmin, Huang, Canhua, Huang, Chuanshu, Huang, Chuanxin, Huang, Chunling, Huang, Haishan, Huang, Kun, Huang, Michael, Huang, Rui, Huang, Shan, Huang, Tianzhi, Huang, Xing, Huang, Yuxiang Jack, Huber, Tobias, Hubert, Virginie, Hubner, Christian, Hughes, Stephanie, 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Chengyu, Liang, Qiangrong, Liang, Weicheng, Liang, Yongheng, Liang, YongTian, Liao, Guanghong, Liao, Lujian, Liao, Mingzhi, Liao, Yung-Feng, Librizzi, Mariangela, Lie, Pearl, Lilly, Mary, Lim, Hyunjung, Lima, Thania, Limana, Federica, Lin, Chao, Lin, Chih-Wen, Lin, Dar-Shong, Lin, Fu-Cheng, Lin, Jiandie, Lin, Kurt, Lin, Kwang-Huei, Lin, Liang-Tzung, LIN, Pei-Hui, Lin, Qiong, Lin, Shaofeng, Lin, Su-Ju, Lin, Wenyu, Lin, Xueying, Lin, Yao-Xin, Lin, Yee-Shin, Linden, Rafael, Lindner, Paula, Ling, Shuo-Chien, Lingor, Paul, Linnemann, Amelia, Liou, Yih-Cherng, Lipinski, Marta, Lipovšek, Saška, Lira, Vitor, Lisiak, Natalia, Liton, Paloma, Liu, Chao, Liu, Ching-Hsuan, Liu, Chun-Feng, Liu, Cui Hua, Liu, Fang, Liu, Hao, Liu, Hsiao-Sheng, Liu, Hua-feng, Liu, Huifang, Liu, Jia, Liu, Jing, Liu, Julia, Liu, Leyuan, Liu, Longhua, Liu, Meilian, Liu, Qin, Liu, Wei, Liu, Wende, Liu, Xiao-Hong, Liu, Xiaodong, Liu, Xingguo, Liu, Xu, Liu, Xuedong, Liu, Yanfen, Liu, Yang, Liu, Yueyang, Liu, Yule, 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Celeste, O’Donovan, Tracey, O’Leary, Seónadh, O’Rourke, Eyleen, O’Sullivan, Mary, O’Sullivan, Timothy, Omary, M. Bishr, Pereira, Gustavo J.S., Ratnayaka, J. Arjuna, Riazuddin, S. Amer, Rouschop, Kasper M.A., Sanderson, J. Thomas, Scaglione, K. Matthew, Schapira, Anthony H.V., Scovassi, A. Ivana, St. Clair, Daret, Sunahara, Karen K.S., Symons, J. David, Triola, Gemma, van Wijk, Sjoerd J.L., Vanrell, M. Cristina, Vasconcelos, M. Helena, Whitton, J. Lindsay, Williams, Robin S.B., Wong, W. Wei-Lynn, Wu, William K.K., Yakhine-Diop, Sokhna M.S., Yu, W. Haung, Zhang, Kalin Y.B., Dong, X. Charlie, Ain Shams University [ASU], Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University [JGU], Medical University Graz, Centre de Recherche des Cordeliers [CRC (UMR_S_1138 / U1138)], Institut Pasteur d'Iran, Leibniz Institute of Plant Biochemistry [IPB], The University of Texas M.D. Anderson Cancer Center [Houston], Institut de pharmacologie moléculaire et cellulaire [IPMC], Lipides - Nutrition - Cancer [Dijon - U1231] [LNC], Centre méditerranéen de médecine moléculaire [C3M], Institut de Biologie Valrose [IBV], Petites Molécules de neuroprotection, neurorégénération et remyélinisation, Signalisation Hormonale, Physiopathologie Endocrinienne et Métabolique, Institut Cochin [IC UM3 (UMR 8104 / U1016)], Institut NeuroMyoGène [INMG], Paris-Centre de Recherche Cardiovasculaire [PARCC (UMR_S 970/ U970)], Marqueurs cardiovasculaires en situation de stress [MASCOT (UMR_S_942 / U942)], Institut de Recherche sur le Cancer et le Vieillissement [IRCAN], Centre de Recherche en Cancérologie de Marseille [CRCM], Centre for Integrative Biology - CBI [Inserm U964 - CNRS UMR7104 - IGBMC], Oncogenesis, Stress, Signaling [OSS], Institut Necker Enfants-Malades [INEM - UM 111 (UMR 8253 / U1151)], Institut de Biologie Intégrative de la Cellule [I2BC], Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte [M2iSH], Centre de Recherches en Cancérologie de Toulouse [CRCT], Physiopathologie et traitement des maladies du foie, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL], Institut de Génétique et de Biologie Moléculaire et Cellulaire [IGBMC], Laboratoire Bio-PeroxIL. Biochimie du peroxysome, inflammation et métabolisme lipidique [Dijon] [BIO-PEROXIL], Centre de recherche sur l'Inflammation [CRI (UMR_S_1149 / ERL_8252 / U1149)], Unité de génétique et biologie des cancers [U830], Laboratoire d'Optique et Biosciences [LOB], Institut des Maladies Métaboliques et Casdiovasculaires [UPS/Inserm U1297 - I2MC], Différenciation et communication neuronale et neuroendocrine [DC2N], Institut de Recherche en Cancérologie de Montpellier [IRCM - U1194 Inserm - UM], Centre d'Immunologie de Marseille - Luminy [CIML], Physiopathologie et imagerie des troubles neurologiques [PhIND], Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée [LBFA], Imagine - Institut des maladies génétiques (IHU) [Imagine - U1163], Institut Mondor de Recherche Biomédicale [IMRB], Franco-czech Laboratory for clinical research on obesity, University of Michigan [Ann Arbor], University of Michigan System, Institut de pharmacologie moléculaire et cellulaire (IPMC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Université Côte d'Azur (UCA), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Institut de Biologie Valrose (IBV), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Sud - Paris 11 (UP11), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP), Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Nutrition, Métabolisme, Aquaculture (NuMéA), Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut NeuroMyoGène (INMG), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Paris-Centre de Recherche Cardiovasculaire (PARCC - UMR-S U970), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Institut de Recherche sur le Cancer et le Vieillissement (IRCAN), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre de Recherche en Cancérologie de Marseille (CRCM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Centre for Integrative Biology - CBI (Inserm U964 - CNRS UMR7104 - IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institute of genetics and molecular and cellular biology-Centre National de la Recherche Scientifique (CNRS), Chemistry, Oncogenesis, Stress and Signaling (COSS), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-CRLCC Eugène Marquis (CRLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Institut des Maladies Neurodégénératives [Bordeaux] (IMN), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherches en Cancérologie de Toulouse (CRCT), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Laboratoire Bio-PeroxIL. Biochimie du peroxysome, inflammation et métabolisme lipidique [Dijon] (BIO-PEROXIL), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre de recherche sur l'Inflammation (CRI (UMR_S_1149 / ERL_8252 / U1149)), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de génétique et biologie des cancers (U830), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Optique et Biosciences (LOB), École polytechnique (X)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Différenciation et communication neuronale et neuroendocrine (DC2N), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM), CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie et imagerie des troubles neurologiques (PhIND), Université de Caen Normandie (UNICAEN), Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Epithelial biology and disease - Liliane Bettencourt Chair of Developmental Biology (Equipe Inserm U1163), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Biomécanique cellulaire et respiratoire (BCR), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Charles University [Prague]-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by the National Institute of General Medical Sciences [GM131919]., Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Marqueurs cardiovasculaires en situation de stress (MASCOT (UMR_S_942 / U942)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Université Sorbonne Paris Nord, Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie des Adaptations Nutritionnelles (PhAN), Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), Département Plateforme (PF I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Gustave Roussy (IGR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Chinese Academy of Medical Sciences [Suzhou, Chine] (CAMS), Karolinska Institutet [Stockholm], Karolinska University Hospital [Stockholm], Department of Women's and Children's Health [Stockholm, Sweden], Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École polytechnique (X), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, FHU OncoAge - Pathologies liées à l’âge [CHU Nice] (OncoAge), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Institut de Pharmacologie Moléculaire et Cellulaire [UNIV Côte d'Azur] (UPMC), Institut Universitaire du Cancer de Toulouse - Oncopole (IUCT Oncopole - UMR 1037), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), Life Sciences Institute [Ann Arbor, MI, USA], University of Michigan System-University of Michigan System, European Institute of Oncology IRCCS [Milan, Italy] (EIO), Ain Shams University (ASU), Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University (JGU), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Réseau International des Instituts Pasteur (RIIP), Leibniz Institute of Plant Biochemistry (IPB), Hebrew University of Jerusalem, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Paris-Centre de Recherche Cardiovasculaire (PARCC (UMR_S 970/ U970)), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Oncogenesis, Stress, Signaling (OSS), Institut des Maladies Métaboliques et Casdiovasculaires (UPS/Inserm U1297 - I2MC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Charles University [Prague] (CU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., Adamopoulos, I. E., Adeli, K., Adolph, T. E., Adornetto, A., Aflaki, E., Agam, G., Agarwal, A., Aggarwal, B. B., Agnello, M., Agostinis, P., Agrewala, J. N., Agrotis, A., Aguilar, P. V., Ahmad, S. T., Ahmed, Z. M., Ahumada-Castro, U., Aits, S., Aizawa, S., Akkoc, Y., Akoumianaki, T., Akpinar, H. A., Al-Abd, A. M., Al-Akra, L., Al-Gharaibeh, A., Alaoui-Jamali, M. A., Alberti, S., Alcocer-Gomez, E., Alessandri, C., Ali, M., Alim Al-Bari, M. A., Aliwaini, S., Alizadeh, J., Almacellas, E., Almasan, A., Alonso, A., Alonso, G. D., Altan-Bonnet, N., Altieri, D. C., Alvarez, E. M. C., Alves, S., Alves da Costa, C., Alzaharna, M. M., Amadio, M., Amantini, C., Amaral, C., Ambrosio, S., Amer, A. O., Ammanathan, V., An, Z., Andersen, S. U., Andrabi, S. A., Andrade-Silva, M., Andres, A. M., Angelini, S., Ann, D., Anozie, U. C., Ansari, M. 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T., Balduini, W., Ballabio, A., Ballester, M., Balazadeh, S., Balzan, R., Bandopadhyay, R., Banerjee, S., Banreti, A., Bao, Y., Baptista, M. S., Baracca, A., Barbati, C., Bargiela, A., Barila, D., Barlow, P. G., Barmada, S. J., Barreiro, E., Barreto, G. E., Bartek, J., Bartel, B., Bartolome, A., Barve, G. R., Basagoudanavar, S. H., Bassham, D. C., Bast, R. C., Basu, A., Batoko, H., Batten, I., Baulieu, E. E., Baumgarner, B. L., Bayry, J., Beale, R., Beau, I., Beaumatin, F., Bechara, L. R. G., Beck, G. R., Beers, M. F., Begun, J., Behrends, C., Behrens, G. M. N., Bei, R., Bejarano, E., Bel, S., Behl, C., Belaid, A., Belgareh-Touze, N., Bellarosa, C., Belleudi, F., Bello Perez, M., Bello-Morales, R., Beltran, J. S. D. O., Beltran, S., Benbrook, D. M., Bendorius, M., Benitez, B. A., Benito-Cuesta, I., Bensalem, J., Berchtold, M. W., Berezowska, S., Bergamaschi, D., Bergami, M., Bergmann, A., Berliocchi, L., Berlioz-Torrent, C., Bernard, A., Berthoux, L., Besirli, C. 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K., Shutt, T. E., Sica, V., Siddiqui, A., Sierra, A., Sierra-Torre, V., Signorelli, S., Sil, P., Silva, B. J. D. A., Silva, J. D., Silva-Pavez, E., Silvente-Poirot, S., Simmonds, R. E., Simon, A. K., Simon, H. -U., Simons, M., Singh, A., Singh, L. P., Singh, R., Singh, S. V., Singh, S. K., Singh, S. B., Singh, S., Singh, S. P., Sinha, D., Sinha, R. A., Sinha, S., Sirko, A., Sirohi, K., Sivridis, E. L., Skendros, P., Skirycz, A., Slaninova, I., Smaili, S. S., Smertenko, A., Smith, M. D., Soenen, S. J., Sohn, E. J., Sok, S. P. M., Solaini, G., Soldati, T., Soleimanpour, S. A., Soler, R. M., Solovchenko, A., Somarelli, J. A., Sonawane, A., Song, F., Song, H. K., Song, J. -X., Song, K., Song, Z., Soria, L. R., Sorice, M., Soukas, A. A., Soukup, S. -F., Sousa, D., Sousa, N., Spagnuolo, P. A., Spector, S. A., Srinivas Bharath, M. M., S, t. Clair D., Stagni, V., Staiano, L., Stalnecker, C. A., Stankov, M. V., Stathopulos, P. B., Stefan, K., Stefan, S. M., Stefanis, L., Steffan, J. S., Steinkasserer, A., Stenmark, H., Sterneckert, J., Stevens, C., Stoka, V., Storch, S., Stork, B., Strappazzon, F., Strohecker, A. M., Stupack, D. G., Su, H., Su, L. -Y., Su, L., Suarez-Fontes, A. M., Subauste, C. S., Subbian, S., Subirada, P. V., Sudhandiran, G., Sue, C. M., Sui, X., Summers, C., Sun, G., Sun, J., Sun, K., Sun, M. -X., Sun, Q., Sun, Y., Sun, Z., Sunahara, K. K. S., Sundberg, E., Susztak, K., Sutovsky, P., Suzuki, H., Sweeney, G., Symons, J. D., Sze, S. C. W., Szewczyk, N. J., Tabecka-Lonczynska, A., Tabolacci, C., Tacke, F., Taegtmeyer, H., Tafani, M., Tagaya, M., Tai, H., Tait, S. W. G., Takahashi, Y., Takats, S., Talwar, P., Tam, C., Tam, S. Y., Tampellini, D., Tamura, A., Tan, C. T., Tan, E. -K., Tan, Y. -Q., Tanaka, M., Tang, D., Tang, J., Tang, T. -S., Tanida, I., Tao, Z., Taouis, M., Tatenhorst, L., Tavernarakis, N., Taylor, A., Taylor, G. A., Taylor, J. M., Tchetina, E., Tee, A. R., Tegeder, I., Teis, D., Teixeira, N., Teixeira-Clerc, F., Tekirdag, K. A., Tencomnao, T., Tenreiro, S., Tepikin, A. V., Testillano, P. S., Tettamanti, G., Tharaux, P. -L., Thedieck, K., Thekkinghat, A. A., Thellung, S., Thinwa, J. W., Thirumalaikumar, V. P., Thomas, S. M., Thomes, P. G., Thorburn, A., Thukral, L., Thum, T., Thumm, M., Tian, L., Tichy, A., Till, A., Timmerman, V., Titorenko, V. I., Todi, S. V., Todorova, K., Toivonen, J. M., Tomaipitinca, L., Tomar, D., Tomas-Zapico, C., Tomic, S., Tong, B. C. -K., Tong, C., Tong, X., Tooze, S. A., Torgersen, M. L., Torii, S., Torres-Lopez, L., Torriglia, A., Towers, C. G., Towns, R., Toyokuni, S., Trajkovic, V., Tramontano, D., Tran, Q. -G., Travassos, L. H., Trelford, C. B., Tremel, S., Trougakos, I. P., Tsao, B. P., Tschan, M. P., Tse, H. -F., Tse, T. F., Tsugawa, H., Tsvetkov, A. S., Tumbarello, D. A., Tumtas, Y., Tunon, M. J., Turcotte, S., Turk, B., Turk, V., Turner, B. J., Tuxworth, R. I., Tyler, J. K., Tyutereva, E. V., Uchiyama, Y., Ugun-Klusek, A., Uhlig, H. H., Ulamek-Koziol, M., Ulasov, I. V., Umekawa, M., Ungermann, C., Unno, R., Urbe, S., Uribe-Carretero, E., Ustun, S., Uversky, V. N., Vaccari, T., Vaccaro, M. I., Vahsen, B. F., Vakifahmetoglu-Norberg, H., Valdor, R., Valente, M. J., Valko, A., Vallee, R. B., Valverde, A. M., Van den Berghe, G., van der Veen, S., Van Kaer, L., van Loosdregt, J., van Wijk, S. J. L., Vandenberghe, W., Vanhorebeek, I., Vannier-Santos, M. A., Vannini, N., Vanrell, M. C., Vantaggiato, C., Varano, G., Varela-Nieto, I., Varga, M., Vasconcelos, M. H., Vats, S., Vavvas, D. G., Vega-Naredo, I., Vega-Rubin-de-Celis, S., Velasco, G., Velazquez, A. P., Vellai, T., Vellenga, E., Velotti, F., Verdier, M., Verginis, P., Vergne, I., Verkade, P., Verma, M., Verstreken, P., Vervliet, T., Vervoorts, J., Vessoni, A. T., Victor, V. M., Vidal, M., Vidoni, C., Vieira, O. V., Vierstra, R. D., Vigano, S., Vihinen, H., Vijayan, V., Vila, M., Vilar, M., Villalba, J. M., Villalobo, A., Villarejo-Zori, B., Villarroya, F., Villarroya, J., Vincent, O., Vindis, C., Viret, C., Viscomi, M. T., Visnjic, D., Vitale, I., Vocadlo, D. J., Voitsekhovskaja, O. V., Volonte, C., Volta, M., Vomero, M., Von Haefen, C., Vooijs, M. A., Voos, W., Vucicevic, L., Wade-Martins, R., Waguri, S., Waite, K. A., Wakatsuki, S., Walker, D. W., Walker, M. J., Walker, S. A., Walter, J., Wandosell, F. G., Wang, B., Wang, C. -Y., Wang, C., Wang, D., Wang, F., Wang, G., Wang, H., Wang, H. -G., Wang, J., Wang, K., Wang, L., Wang, M. H., Wang, M., Wang, N., Wang, P., Wang, Q. J., Wang, Q., Wang, Q. K., Wang, Q. A., Wang, W. -T., Wang, W., Wang, X., Wang, Y., Wang, Y. -Y., Wang, Z., Warnes, G., Warnsmann, V., Watada, H., Watanabe, E., Watchon, M., Wawrzynska, A., Weaver, T. E., Wegrzyn, G., Wehman, A. M., Wei, H., Wei, L., Wei, T., Wei, Y., Weiergraber, O. H., Weihl, C. C., Weindl, G., Weiskirchen, R., Wells, A., Wen, R. H., Wen, X., Werner, A., Weykopf, B., Wheatley, S. P., Whitton, J. L., Whitworth, A. J., Wiktorska, K., Wildenberg, M. E., Wileman, T., Wilkinson, S., Willbold, D., Williams, B., Williams, R. S. B., Williams, R. L., Williamson, P. R., Wilson, R. A., Winner, B., Winsor, N. J., Witkin, S. S., Wodrich, H., Woehlbier, U., Wollert, T., Wong, E., Wong, J. H., Wong, R. W., Wong, V. K. W., Wong, W. W. -L., Wu, A. -G., Wu, C., Wu, J., Wu, K. K., Wu, M., Wu, S. -Y., Wu, S., Wu, W. K. K., Wu, X., Wu, Y. -W., Wu, Y., Xavier, R. J., Xia, H., Xia, L., Xia, Z., Xiang, G., Xiang, J., Xiang, M., Xiang, W., Xiao, B., Xiao, G., Xiao, H., Xiao, H. -T., Xiao, J., Xiao, L., Xiao, S., Xiao, Y., Xie, B., Xie, C. -M., Xie, M., Xie, Y., Xie, Z., Xilouri, M., Xu, C., Xu, E., Xu, H., Xu, J., Xu, L., Xu, W. W., Xu, X., Xue, Y., Yakhine-Diop, S. M. S., Yamaguchi, M., Yamaguchi, O., Yamamoto, A., Yamashina, S., Yan, S., Yan, S. -J., Yan, Z., Yanagi, Y., Yang, C., Yang, D. -S., Yang, H., Yang, H. -T., Yang, J. -M., Yang, J., Yang, L., Yang, M., Yang, P. -M., Yang, Q., Yang, S., Yang, S. -F., Yang, W., Yang, W. Y., Yang, X., Yang, Y., Yao, H., Yao, S., Yao, X., Yao, Y. -G., Yao, Y. -M., Yasui, T., Yazdankhah, M., Yen, P. M., Yi, C., Yin, X. -M., Yin, Y., Yin, Z., Ying, M., Ying, Z., Yip, C. K., Yiu, S. P. T., Yoo, Y. H., Yoshida, K., Yoshii, S. R., Yoshimori, T., Yousefi, B., Yu, B., Yu, H., Yu, J., Yu, L., Yu, M. -L., Yu, S. -W., Yu, V. C., Yu, W. H., Yu, Z., Yuan, J., Yuan, L. -Q., Yuan, S., Yuan, S. -S. F., Yuan, Y., Yuan, Z., Yue, J., Yue, Z., Yun, J., Yung, R. L., Zacks, D. N., Zaffagnini, G., Zambelli, V. O., Zanella, I., Zang, Q. S., Zanivan, S., Zappavigna, S., Zaragoza, P., Zarbalis, K. S., Zarebkohan, A., Zarrouk, A., Zeitlin, S. O., Zeng, J., Zeng, J. -D., Zerovnik, E., Zhan, L., Zhang, B., Zhang, D. D., Zhang, H., Zhang, H. -L., Zhang, J., Zhang, J. -P., Zhang, K. Y. B., Zhang, L. W., Zhang, L., Zhang, M., Zhang, P., Zhang, S., Zhang, W., Zhang, X., Zhang, X. -W., Zhang, X. D., Zhang, Y., Zhang, Y. -D., Zhang, Y. -Y., Zhang, Z., Zhao, H., Zhao, L., Zhao, S., Zhao, T., Zhao, X. -F., Zhao, Y., Zheng, G., Zheng, K., Zheng, L., Zheng, S., Zheng, X. -L., Zheng, Y., Zheng, Z. -G., Zhivotovsky, B., Zhong, Q., Zhou, A., Zhou, B., Zhou, C., Zhou, G., Zhou, H., Zhou, J., Zhou, K., Zhou, R., Zhou, X. -J., Zhou, Y., Zhou, Z. -Y., Zhou, Z., Zhu, B., Zhu, C., Zhu, G. -Q., Zhu, H., Zhu, W. -G., Zhu, Y., Zhuang, H., Zhuang, X., Zientara-Rytter, K., Zimmermann, C. M., Ziviani, E., Zoladek, T., Zong, W. -X., Zorov, D. B., Zorzano, A., Zou, W., Zou, Z., Zuryn, S., Zwerschke, W., Brand-Saberi, B., Dong, X. C., Kenchappa, C. S., Lin, Y., Oshima, S., Rong, Y., Sluimer, J. C., Stallings, C. L., Tong, C. -K., and Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Programmed cell death, Settore BIO/06, Autophagosome, Autolysosome, [SDV]Life Sciences [q-bio], lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4], Autophagy-Related Proteins, Review, Computational biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Biology, Settore MED/04, 03 medical and health sciences, stress, Chaperone-mediated autophagy, ddc:570, Autophagy, LC3, Animals, Humans, cancer, Settore BIO/10, flux, lysosome, macroautophagy, neurodegeneration, phagophore, vacuole, Set (psychology), Molecular Biology, 030102 biochemistry & molecular biology, business.industry, Interpretation (philosophy), Autophagosomes, Cell Biology, Multicellular organism, 030104 developmental biology, Knowledge base, Biological Assay, Lysosomes, business, Biomarkers, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: Contains fulltext : 232759.pdf (Publisher’s version ) (Closed access) In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
Published: 2021
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17. Effects of chronic exposure to bisphenol A in adult female mice on social behavior, vasopressin system, and estrogen membrane receptor (GPER1)

Author: Martina Bettarelli, Stefano Gotti, Brigitta Bonaldo, Antonino Casile, Giancarlo Panzica, and Marilena Marraudino
Subjects: Male, Vasopressin, vasopressin, Endocrine Disruptors, GPER1, Receptors, G-Protein-Coupled, Three-Chamber Test, Pregnancy, Lactation, Biology (General), Behavior, Animal, BPA, Endocrine disrupting chemicals, BPA, Three-Chamber test, vasopressin, oxytocin, GPER1, paraventricular nucleus, suprachiasmatic nucleus, medicine.anatomical_structure, Receptors, Estrogen, Female, paraventricular nucleus, hormones, hormone substitutes, and hormone antagonists, medicine.drug, medicine.medical_specialty, endocrine system, Histology, medicine.drug_class, Vasopressins, QH301-705.5, Biophysics, Estrous Cycle, Biology, Article, Phenols, Internal medicine, oxytocin, medicine, Endocrine system, Animals, Benzhydryl Compounds, Social Behavior, Estrous cycle, urogenital system, suprachiasmatic nucleus, Cell Biology, medicine.disease, endocrine disrupting chemicals, Mice, Inbred C57BL, Endocrinology, Oxytocin, Estrogen, Hormone, Paraventricular Hypothalamic Nucleus
Abstract: Bisphenol A (BPA), an organic synthetic compound found in some plastics and epoxy resins, is classified as an endocrine disrupting chemical. Exposure to BPA is especially dangerous if it occurs during specific “critical periods” of life, when organisms are more sensitive to hormonal changes (i.e., intrauterine, perinatal, juvenile or puberty periods). In this study, we focused on the effects of chronic exposure to BPA in adult female mice starting during pregnancy. Three months old C57BL/6J females were orally exposed to BPA or to vehicle (corn oil). The treatment (4 µg/kg body weight/day) started the day 0 of pregnancy and continued throughout pregnancy, lactation, and lasted for a total of 20 weeks. BPA-treated dams did not show differences in body weight or food intake, but they showed an altered estrous cycle compared to the controls. In order to evidence alterations in social and sociosexual behaviors, we performed the Three-Chamber test for sociability, and analyzed two hypothalamic circuits (well-known targets of endocrine disruption) particularly involved in the control of social behavior: the vasopressin and the oxytocin systems. The test revealed some alterations in the displaying of social behavior: BPA-treated dams have higher locomotor activity compared to the control dams, probably a signal of high level of anxiety. In addition, BPA-treated dams spent more time interacting with no-tester females than with no-tester males. In brain sections, we observed a decrease of vasopressin immunoreactivity (only in the paraventricular and suprachiasmatic nuclei) of BPA-treated females, while we did not find any alteration of the oxytocin system. In parallel, we have also observed, in the same hypothalamic nuclei, a significant reduction of the membrane estrogen receptor GPER1 expression.
Published: 2021

18. Taxonomic Revision of the Spider Genus Actinopus Perty, 1833 (Araneae, Mygalomorphae, Actinopodidae)

Author: Laura Tavares Miglio, Fernando Pérez-Miles, Alexandre B. Bonaldo, Miglio L.T., Pérez-Miles Fernando, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología., and Bonaldo A.B.
Subjects: Morphology, Pharmacology, Zoology, Actinopodidae, Biology, biology.organism_classification, Liodon, Neotropical, Mygalomorphae, New species, Type species, Genus, Actinopus, Key (lock), Taxonomy (biology), Geographic distribution, Taxonomy
Abstract: Actinopus Perty, 1833 is characterized and redescribed, including 80 species occurring from Panama to Argentina. Eighteen previously known species are redescribed: A. rufipes (Lucas, 1834); A. longipalpis C. L. Koch, 1842; A. nattereri (Doleschall, 1871); A. crassipes (Keyserling, 1891); A. robustus (O. Pickard-Cambridge, 1892); A. wallacei F. O. P.-Cambridge, 1896; A. princeps Chamberlin, 1917; A. xenus Chamberlin, 1917; A. fractus Mello-Leitão, 1920; A. paranensis Mello-Leitão, 1920; A. pusillus Mello-Leitão, 1920; A. dubiomaculatus Mello-Leitão, 1923; A. trinotatus Mello-Leitão, 1938; A. cucutaensis Mello-Leitão, 1941; A. echinus Mello-Leitão, 1949; A. clavero Ríos-Tamayo & Goloboff, 2018; A. gerschiapelliarum Ríos-Tamayo & Goloboff, 2018; and A. palmar Ríos-Tamayo & Goloboff, 2018. Additionally, A. tarsalis Perty, 1833, the type species, A. insignis (Holmberg, 1881) and other 18 species recently described species from Argentina and Bolivia, are re-evaluated. Forty-two new species are described: Actinopus castelo sp. nov., A. apalai sp. nov., A. mairinquensis sp. nov., A. obidos sp. nov., A. buritiensis sp. nov., A. pinhao sp. nov., A. ducke sp. nov., A. hirsutus sp. nov., A. jaboticatubas sp. nov., A. confusus sp. nov., A. pampulha sp. nov., A. candango sp. nov., A. paraitinga sp. nov., A. cornelli sp. nov., A. vilhena sp. nov., A. harveyi sp. nov., A. itapitocai sp. nov., A. ipioca sp. nov., A. itaqui sp. nov., A. xingu sp. nov., A. mesa sp. nov., A. caxiuana sp. nov., A. utinga sp. nov., A. emas sp. nov., A. bocaina sp. nov., A. osbournei sp. nov., A. dioi sp. nov., A. tutu sp. nov., A. azaghal sp. nov., A. itacolomi sp. nov., A. parafundulus sp. nov., A. urucui sp. nov., A. reznori sp. nov., A. anselmoi sp. nov., A. guajara sp. nov., A. apiacas sp. nov., A. jamari sp. nov. from Brazil; A. laventana sp. nov. from Argentina and Uruguay; A. lomalinda sp. nov. from British Guiana and Colombia; A. concinnus sp. nov. from Venezuela and Brazil; A. pindapoy sp. nov. from Argentina; A. panguana sp. nov. from Peru. The females of A. dubiomaculatus and A. cucutaensis and the males of A. nattereri are described for the first time. New records are presented for A. clavero; A.crassipes; A. dubiomaculatus; A. fractus; A. gerschiapelliarum; A. nattereri; A. palmar; A. paranensis; A. princeps, A. pusillus, A. robustus and A. wallacei. Ten old species names are herein considered species inquirendae: A. caraiba (Simon, 1889); A. harti Pocock, 1895; A. liodon (Ausserer, 1875); A. nigripes (Lucas, 1834); A. pertyi Lucas, 1843; A. piceus (Ausserer, 1871); A. rojasi (Simon, 1889); A. rufibarbis Mello-Leitão, 1930; A. scalops (Simon, 1889) and A. valencianus (Simon, 1889). Most species presently recognized were included in 11 informal groups based mainly on male palpal characters. A key for these groups plus three species, not included in any group, but known from males, is presented.
Published: 2020
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19. Effects of dietary organic acids and nature identical compounds on growth, immune parameters and gut microbiota of European sea bass

Author: Ester Grilli, Sara Ciulli, Marco Candela, Alessio Bonaldo, Matteo Soverini, Federica D’Amico, Pier Paolo Gatta, Luca Parma, Andrea Piva, Enrico Volpe, Serena Busti, Barbara Rossi, Busti S., Rossi B., Volpe E., Ciulli S., Piva A., D'Amico F., Soverini M., Candela M., Gatta P.P., Bonaldo A., Grilli E., and Parma L.
Subjects: 0301 basic medicine, Molecular biology, medicine.medical_treatment, Gut flora, chemistry.chemical_compound, Transforming Growth Factor beta, Lactobacillus, Animal physiology, Food science, Thymol, Multidisciplinary, biology, High-Throughput Nucleotide Sequencing, 04 agricultural and veterinary sciences, Biodiversity, Interleukin-10, immune parameter, Medicine, Cytokines, Mucosal immunology, Proteobacteria, Science, growth, Immunology, Microbial communities, Microbiology, Article, European sea ba, 03 medical and health sciences, functional feed, medicine, Leuconostoc, Animals, cytokines gene expression, Sea bass, organic acid, gut microbiota, Prebiotic, Interleukin-8, biology.organism_classification, Gastrointestinal Microbiome, botanical, 030104 developmental biology, chemistry, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Bass, Sorbic acid, Zoology
Abstract: A 71-day study was conducted to explore the effect of increasing dietary levels (0, 250, 500, 1000 mg kg feed−1; D0, D250, D500 and D1000, respectively) of a blend of microencapsulated organic acids (OA, specifically citric and sorbic acid) and nature identical compounds (NIC, specifically thymol and vanillin), on growth, intestinal immune parameters and gut microbiota (GM) of European sea bass juveniles reared under normal and subsequently suboptimal environmental conditions (high temperature, 30.0 ± 0.4 °C and low oxygen, 4.6 ± 0.6 mg L−1). OA and NIC did not promote growth, feed utilisation and feed intake at the inclusion tested but induced a significantly upregulation of IL-8, IL-10 and TGFβ. GM analyzed by next-generation sequencing showed that OA and NIC were able to exert prebiotic properties stimulating the development of beneficial bacteria taxa such as Lactobacillus, Leuconostoc, and Bacillus sp. Picrust analyses displayed a significant potential functional reconfiguration of GM promoting a decrease in inflammation-promoting and homeostatic functions at increasing OA and NIC administration. For the first time on this species the exposure to suboptimal rearing conditions was able to modify GM structure reducing LAB and increasing Proteobacteria, findings which were consistent with the inflammatory process observed at mRNA level.
Published: 2020

20. Monitoring of common sole Solea Solea (L) captive broodstock from Northern Adriatic Sea over consecutive spawning seasons

Author: Ilaria Guarniero, Luca Parma, Alessio Bonaldo, Pier Paolo Gatta, Cinzia Viroli, Alessia Cariani, Claudia Ofelio, Ofelio C., Guarniero I., Cariani A., Viroli C., Bonaldo A., Gatta P.P., and Parma L.
Subjects: Common sole, media_common.quotation_subject, Zoology, Captivity, Broodstock, Aquaculture, Aquatic Science, Biology, Mediterranean, lcsh:Aquaculture. Fisheries. Angling, 03 medical and health sciences, Seasonal breeder, Parentage contribution, 030304 developmental biology, media_common, lcsh:SH1-691, 0303 health sciences, Soleá, business.industry, Reproduction, Solea solea, 04 agricultural and veterinary sciences, Fecundity, biology.organism_classification, STRs, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Animal Science and Zoology, business
Abstract: The high nutritional quality of common sole Solea solea increases its value for the fishery industry and for the aquaculture sector. To ensure the expansion of its production, it is necessary to implement farming and broodstock management technique to produce high quality eggs and larvae. This work summarizes eight years of study on reproductive performances, growth and parental contribution of a common sole broodstock from the Mediterranean-North Adriatic Sea. The broodstock (11 females and 13 males) reached the peak of fecundity after 5/6 years of captivity, with a production of 296.48 and 376.54 × 103 of total eggs kg−1 female with a fertilization rate of 31.6 ± 18.3 and 41.9 ± 23.8 %, respectively. Results shows that variations in temperature cycles are pivotal for a successful breeding season, and body condition during the first 3 years of captivity was the most important parameter positively correlated to parental contribution for both males and females. Parental contribution was assessed by eight species-specific microsatellite loci during the first 3 years of reproduction. Although the two sexes displayed different temporal trends, parentage assessment demonstrated an overall increase in the number of active breeders. However, only 13 couples out of 50 produced the 70.4 % of the larvae in year 5, confirming the same reproduction behavioural pattern in Mediterranean common sole than that observed both in Senegalese sole and in common sole of Atlantic origin. This work provides valuable data needed to understand the mechanisms behind the maturation characteristics of this species and support future strategy for the establishment and management of Mediterranean common sole aquaculture.
Published: 2020

21. Effects of rearing density on growth, digestive conditions, welfare indicators and gut bacterial community of gilthead sea bream (Sparus aurata, L. 1758) fed different fishmeal and fish oil dietary levels

Author: María Ángeles Esteban, Luca Parma, Alessio Bonaldo, Francesco Dondi, Pier Paolo Gatta, Enric Gisbert, Nicole Francesca Pelusio, Federica D’Amico, Marco Candela, Matteo Soverini, Luca Parma, Nicole Francesca Pelusio, Enric Gisbert, Maria Angeles Esteban, Federica D'Amico, Matteo Soverini, Marco Candela, Francesco Dondi, Pier Paolo Gatta, Alessio Bonaldo, Producció Animal, and Aqüicultura
Subjects: Aquatic Science, Gut flora, Quantitative Biology - Quantitative Methods, Feed conversion ratio, 03 medical and health sciences, chemistry.chemical_compound, Animal science, Fish meal, Stocking, Aquaculture, 14. Life underwater, Gilthead sea bream, rearing density, fishmeal and fish oil replacement, digestive enzyme, humoral immunity on skin mucus, gut bacterial community, Quantitative Methods (q-bio.QM), 030304 developmental biology, 2. Zero hunger, 0303 health sciences, biology, business.industry, 04 agricultural and veterinary sciences, biology.organism_classification, Fish oil, chemistry, FOS: Biological sciences, Digestive enzyme, 040102 fisheries, biology.protein, 0401 agriculture, forestry, and fisheries, Lysozyme, business
Abstract: In Mediterranean aquaculture, significant advances have been made towards a reduction of marine-derived ingredients in aquafeed formulation, as well as in defining the effect on how environmental factors such as rearing density interact with fish health. Little research, however, has examined the interaction between rearing density and dietary composition on main key performance indicators, physiological processes and gut bacterial community. A study was undertaken, therefore to assess growth response, digestive enzyme activity, humoral immunity on skin mucus, plasma biochemistry and gut microbiota of gilthead sea bream (Sparus aurata, L. 1758) reared at high (HD, 36–44 kg m−3) and low (LD, 12–15 kg m−3) final stocking densities and fed high (FM30/FO15, 30% fishmeal FM, 15% fish oil, FO) and low (FM10/FO3; 10% FM and 3% FO) FM and FO levels. Isonitrogenous and isolipidic extruded diets were fed to triplicate fish groups (initial weight: 96.2 g) to overfeeding over 98 days. The densities tested had no major effects on overall growth and feed efficiency of sea bream reared at high or low FM and FO dietary level. However, HD seems to reduce feed intake compared to LD mainly in fish fed FM30/FO15. Results of digestive enzyme activity indicated a comparable digestive efficiency among rearing densities and within each dietary treatment even if intestinal brush border enzymes appeared to be more influenced by stocking density compared to gastric and pancreatic enzymes. Plasma parameters related to nutritional and physiological conditions were not affected by rearing densities under both nutritional conditions a similar observation was also achieved through the study of lysozyme, protease, antiprotease and total protein determination in skin mucus, however; in this case lysozyme was slightly reduced at HD. For the first time on this species, the effect of rearing density on gut bacterial community was studied. Different response in relation to dietary treatment under HD and LD were detected. Low FM-FO diet maintained steady the biodiversity of the gut bacterial community between LD and HD conditions while fish fed high FM-FO level showed a reduced biodiversity at HD. According to the results, it seems feasible to rear gilthead sea bream at the on-growing phase at a density up to 36–44 kg m−3 with low or high FM-FO diet without negatively affecting growth, feed efficiency, welfare condition and gut bacterial community. info:eu-repo/semantics/acceptedVersion
Published: 2020

22. First insights on Black Soldier Fly (Hermetia illucens L.) larvae meal dietary administration in Siberian sturgeon (Acipenser baerii Brandt) juveniles

Author: Marino Prearo, Christian Caimi, Francesco Gai, Laura Gasco, Alessio Bonaldo, Antonia Concetta Elia, Marta Gariglio, Carola Lussiana, Achille Schiavone, Marco Meneguz, Manuela Renna, Sihem Dabbou, Caimi C., Renna M., Lussiana C., Bonaldo A., Gariglio M., Meneguz M., Dabbou S., Schiavone A., Gai F., Elia A.C., Prearo M., and Gasco L.
Subjects: chemistry.chemical_classification, Meal, Hermetia illucens, biology, Fishmeal substitution, Fatty acid, Acipenser baerii, Aquatic Science, Proximate, Animal performance, Apparent digestibility coefficient, Fatty acid profile, Insect meal, biology.organism_classification, Animal science, Fish meal, Sturgeon, chemistry, Dry matter
Abstract: The Hermetia illucens (L.) larvae meal (HIM) has been tested on different fish species but its use on Siberian sturgeon has not been investigated yet. The aim of this study was to evaluate the effects of fish meal (FM) substitution with a highly defatted HIM on growth performance, biometric and morphometric indices, apparent digestibility of diets, whole body proximate and fatty acid compositions of Acipenser baerii (Brandt) juveniles. Five experimental groups were fed with a FM-based diet without HIM (HIM0), three diets with 25% (HIM25), 50% (HIM50) and 100% (HIM100) of FM substitution with HIM, or a vegetable protein based diet (VEG) without HIM. The feeding trial lasted 118 days and 4 replicates per diet were used. The HIM100 diet was refused by the fish and therefore this experimental group was excluded from statistical evaluation. Moreover, a decrease in feed consumption was recorded with the increase of HIM inclusion. The HIM50 diet negatively affected the growth performance of the fish. The somatic indices were not affected by treatment. Increasing levels of HIM showed increases of dry matter and ether extract contents in the whole body. Compared to HIM0, HIM diets caused modification in lauric acid (up to 65-fold increase) and total saturated fatty acids (up to 1.4-fold increase) contents in the fish whole body. The apparent digestibility coefficients of dry matter, crude protein and gross energy were the highest for the VEG diet (77.0%, 90.4% and 85.8%, respectively). HIM25 and HIM50 showed lower apparent digestibility coefficients of crude protein (86.5% and 86.6%, respectively) when compared to HIM0 (88.5%). Overall, this study showed that it is possible to replace up to 25% of FM with HIM in the diet of Siberian sturgeons (equal to 18.5% HIM inclusion level) without affecting the growth performance, condition factor, biometric and morphometric indices, and whole body proximate composition of the fish. The fatty acid composition of the sturgeons’ whole body was significantly modified already at 18.5% dietary HIM inclusion level.
Published: 2020
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23. Toward the Authentication of European Sea Bass Origin through a Combination of Biometric Measurements and Multiple Analytical Techniques

Author: Federica Farabegoli, Emanuela Zanardi, Luca Parma, Marina Silvi, Maurizio Pirini, Silvia Testi, Magda Rotolo, Alessio Bonaldo, Sergio Ghidini, Anna Badiani, Daniel Remondini, Farabegoli, Federica, Pirini, Maurizio, Rotolo, Magda, Silvi, Marina, Testi, Silvia, Ghidini, Sergio, Zanardi, Emanuela, Remondini, Daniel, Bonaldo, Alessio, Parma, Luca, and Badiani, Anna
Subjects: Multivariate analysis, Biometrics, Food Contamination, Biology, 01 natural sciences, fatty acid profile, 0404 agricultural biotechnology, Stocking, sea bass authentication, Statistics, seafood labeling, Animals, Dicentrarchus labrax, Sea bass, Isotope analysis, Authentication, Chemistry (all), Fatty Acids, 010401 analytical chemistry, analytical fingerprinting, stocking density, Discriminant Analysis, isotope analysi, 04 agricultural and veterinary sciences, General Chemistry, Fish products, biology.organism_classification, 040401 food science, 0104 chemical sciences, Europe, Agricultural and Biological Sciences (all), Seafood, elemental composition, Biometric Identification, Multivariate Analysis, Bass, Dicentrarchus, General Agricultural and Biological Sciences
Abstract: The authenticity of fish products has become an imperative issue for authorities involved in the protection of consumers against fraudulent practices and market stabilization. The present study aimed to provide a method for authentication of European sea bass ( Dicentrarchus labrax) according to the requirements for seafood labels (Regulation 1379/2013/EU). Data on biometric traits, fatty acid profile, elemental composition, and isotopic abundance of wild and reared (intensively, semi-intensively, and extensively) specimens from 18 southern European sources ( n = 160) were collected, clustered in six sets of parameters, and then subjected to multivariate analysis. Correct allocations of subjects according to their production method, origin, and stocking density were demonstrated with good approximation rates (94, 92, and 92%, respectively) using fatty acid profiles. Less satisfying results were obtained using isotopic abundance, biometric traits, and elemental composition. The multivariate analysis also revealed that extensively reared subjects cannot be analytically discriminated from wild subjects.
Published: 2018
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24. Integrated study on production performance and quality traits of European sea bass (Dicentrarchus labrax) fed high plant protein diets

Author: Eleonora Nannoni, Alessio Bonaldo, Erika Bonvini, Anna Badiani, Ramon Fontanillas, Pier Paolo Gatta, Federico Sirri, Luca Parma, Bonvini, Erika, Parma, Luca, Badiani, Anna, Fontanillas, Ramon, Gatta, PIER PAOLO, Sirri, Federico, Nannoni, Eleonora, and Bonaldo, Alessio
Subjects: 0301 basic medicine, Fish farming, Growth, Aquatic Science, Biology, Body weight, Feed conversion ratio, European sea ba, Plant protein, 03 medical and health sciences, Animal science, Fish meal, Sea bass, Fishmeal, Fillet, business.industry, 04 agricultural and veterinary sciences, Skin colour, biology.organism_classification, Quality, Biotechnology, 030104 developmental biology, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Dicentrarchus, business
Abstract: In the issue of fishmeal replacement, besides maintaining optimal growth, a key area of investigation for continuing to improve modern aquafeeds includes the evaluation of the effects of plant ingredients on fish quality. It is generally accepted that farmed fish quality can be influenced by the formulation of composition of their feed. Hence, the aim of the present research was to evaluate plant protein inclusion up to 84% of the overall protein content in an integrated study on growth and quality traits of European sea bass. Three diets were formulated to contain increasing plant protein levels (50, 67 and 84%; 50PP, 67PP and 84PP, respectively), with fishmeal dietary levels at 30, 20 and 10%, respectively. No significant differences due to reducing fishmeal content were observed after 118 days in terms of growth (final body weight and specific growth rate) and feed intake, even though a trend towards lower growth performance at higher fishmeal replacement levels was observed. Fish fed diet 50PP showed lower feed conversion rate in comparison to those fed diet 84PP, while no differences were recorded between diet 50PP and 67PP. No significant differences among treatments were found in protein efficiency rate. On the contrary, fish fed diet 84PP showed lower gross protein efficiency in comparison to those fed diet 50PP and 67PP. No significant differences in biometric indices and fillet composition were observed. No significant differences were found in pH, liquid holding capacity and skin colour measurements between treatments, while regarding fillet colour, significant differences were found only for H°ab. In conclusion, our findings demonstrated that dietary plant proteins up to 84% of the overall protein content had no effects on quality traits of European sea bass in comparison with 50% and 67%. All experimental groups showed similar growth even though 84% plant protein inclusion negatively influenced feed and protein utilisation.
Published: 2018
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25. The spider genus Medionops Sánchez-Ruiz & Brescovit (Araneae: Caponiidae) in Colombia, with the description of four new species

Author: Alexander Sánchez-Ruiz, Alexandre B. Bonaldo, and Leonel Martínez
Subjects: Arthropoda, Zoology, Identification key, ddc:590, Genus, Arachnida, Animalia, Neotropical region, Ecology, Evolution, Behavior and Systematics, Taxonomy, Spider, biology, Botany, Caponiidae, Biodiversity, Synspermiata, biology.organism_classification, Type species, Geography, Nopinae, QL1-991, QK1-989, Araneae, Taxonomy (biology)
Abstract: Four new Colombian species of the spider genus Medionops Sánchez-Ruiz & Brescovit, 2017 are described and illustrated: M. carolinae sp. nov. (male and female) from Boyacá department, M. cauca sp. nov. (male) from Cauca department, M. luiscarlosi sp. nov. (male and female) from Caldas department and M. santarosa sp. nov. (male) from Risaralda department. Additionally, the distribution of the type species of M. blades Sánchez-Ruiz & Brescovit, 2017, previously known only from Bogotá, Cundinamarca department, is extended to several localities in the Boyacá department. An updated identification key for all Medionops species is provided.
Published: 2021

26. Genotype-specific features reduce the susceptibility of South American yellow fever virus strains to vaccine-induced antibodies

Author: Mrunal Sakharkar, Esper G. Kallas, Gorka Lasso, Lidiane Menezes Souza Raphael, Alexandra Tse, Kartik Chandran, Anna Z. Wec, Laura M. Walker, Nuria Pedreño-Lopez, Simone Sidoli, Zachary A. Bornholdt, Carolina Argondizo Correia, Yan Sun, Nathália Dias Furtado, Vivian Iida Avelino-Silva, Stephanie Stransky, Crystal L. Moyer, Myrna C. Bonaldo, Denise Haslwanter, and David I. Watkins
Subjects: biology, Genotype, Strain (biology), Yellow fever, Yellow Fever Vaccine, Virulence, medicine.disease, Antibodies, Viral, Vaccines, Attenuated, Virology, Microbiology, Virus, Article, Vaccination, Polyclonal antibodies, Yellow Fever, biology.protein, medicine, Humans, Parasitology, Antibody, Yellow fever virus, Brazil
Abstract: SummaryThe resurgence of yellow fever in South America has prompted mitigation through vaccination against the etiologic agent, yellow fever virus (YFV). Current vaccines are based on a virulent African isolate, and their capacity to induce neutralizing antibodies against the vaccine strain is widely used as a surrogate for protection. However, the sensitivity of genetically distinct South American strains to vaccine-induced antibodies is unknown. Here, we show that antiviral potency of the polyclonal antibody response in both U.S. and Brazilian vaccinees is attenuated against an emergent Brazilian strain. This reduction was attributable to genetic changes at two sites in the central domain II of the glycoprotein E, including the acquisition of an N–linked glycosylation site, which are unique to and shared among most South American YFV strains. Our findings call for a reevaluation of current approaches to YFV immunological surveillance in South America and suggest approaches for designing updated vaccines.
Published: 2021

27. Doxycycline Inhibition of a Pseudotyped Virus Transduction Does Not Translate to Inhibition of SARS-CoV-2 Infectivity

Author: Arianna Piotti, Federica Filippini, Luisa Diomede, Alessandro Corbelli, Massimo Pizzato, Fabio Fiordaliso, Jacopo Lucchetti, Sara Baroni, Ada De Luigi, Francesca Caccuri, Mario Salmona, Marten Beeg, Arnaldo Caruso, Valerio Bonaldo, Nicolò Panini, Claudia Fracasso, Luca Russo, Marco Gobbi, and Emiliano Biasini
Subjects: viruses, Virus Replication, spike protein, Transduction (genetics), Transduction, Genetic, Chlorocebus aethiops, Medicine, Receptor, Doxycycline, Infectivity, COVID-19, In vitro, SARS-CoV-2, Spike protein, Surface plasmon resonance, Tetracyclines, Angiotensin-Converting Enzyme 2, Animals, Cell Cycle, HEK293 Cells, Humans, Protein Binding, Spike Glycoprotein, Coronavirus, Vero Cells, Virus Physiological Phenomena, Host-Pathogen Interactions, in vitro, Cell cycle, tetracyclines, doxycycline, surface plasmon resonance, QR1-502, Spike Glycoprotein, Infectious Diseases, medicine.drug, Coronavirus disease 2019 (COVID-19), Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Biology, Microbiology, Virus, Article, Transduction, Genetic, Virology, business.industry, HEK 293 cells, Clinical trial, Coronavirus, Vero cell, business
Abstract: The pandemic caused by the SARS-CoV-2 has created the need of compounds able to interfere with the biological processes exploited by the virus. Doxycycline, with its pleiotropic effects, including anti-viral activity, has been proposed as a therapeutic candidate for COVID-19 and about twenty clinical trials have started since the beginning of the pandemic.To gain information on the activity of doxycycline against SARS-CoV-2 infection and clarify some of the conflicting clinical data published, we designed in vitro binding tests and infection studies with a pseudotyped virus expressing the spike protein, as well as a clinically isolated SARS-CoV-2 strain. Doxycycline inhibited the transduction of the pseudotyped virus in Vero E6 and HEK-293 T cells stably expressing human receptor angiotensin-converting enzyme 2 but did not affect the entry and replication of SARS-CoV-2.Although this conclusion is apparently disappointing, it is paradigmatic of an experimental approach aimed at developing an integrated multidisciplinary platform. To avoid wasting precious time and resources we believe very stringent experimental criteria are needed in the preclinical phase, including infectious studies with SARS-CoV-2 in the platform before moving on to [failed] clinical trials.Author SummaryThe pandemic caused by the SARS-CoV-2 virus has created a completely unusual situation in rapidly searching for compounds able to interfere with the biological processes exploited by the virus. This new scenario has substantially changed the timing of drug development which has also resulted in the generation of controversial results, proving that the transition from computational screening to the clinical application requires great caution and careful studies. It is therefore necessary to establish new paradigms for evaluating the efficacy of a potential active molecule.We set up a preclinical platform aimed at identifying molecules active against SARS-CoV-2 infection developing a multidisciplinary approach based on very stringent experimental criteria, comprising in-silico studies, in vitro binding tests and infection studies with pseudovirus expressing the spike protein as well as clinically isolated SARS-CoV-2 strains. We focused our attention on doxycycline which has been suggested as potential therapeutic candidate for treating COVID-19 and is currently employed in about twenty clinical trials. Doxycycline resulted effective in inhibiting the transduction of pseudovirus but it did not affect the entry and replication of SARS-CoV-2. The results obtained underline the need to define more stringent and controlled pharmacological approaches before wasting precious time and resources with clinical trials.
Published: 2021

28. Taxonomic revision of the genus Tupirinna Bonaldo, 2000 (Araneae: Corinnidae: Corinninae)

Author: Alexandre B. Bonaldo and Cláudia Xavier
Subjects: 0106 biological sciences, Male, Species groups, Corinnidae, Arthropoda, Espirito santo, 010607 zoology, Identification key, Zoology, Northeast brazil, 010603 evolutionary biology, 01 natural sciences, Genus, Arachnida, Animalia, Animals, Genitalia, Ecology, Evolution, Behavior and Systematics, Taxonomy, biology, Dionycha, Spiders, Biodiversity, biology.organism_classification, Araneae, Animal Science and Zoology, Taxonomy (biology), Female, Animal Distribution, Brazil
Abstract: The genus Tupirinna Bonaldo, 2000 is revised, including 20 species. New records of T. rosae Bonaldo, 2000 from Pará, Brazil are given. Tupirinna albofasciata (Mello-Leitão, 1943) is redescribed based on the female lectotype, here designated. The following 17 new species are described, diagnosed, and illustrated: T. platnicki sp. nov. (♂ and ♀ from Pará, Brazil); T. zebra sp. nov. (♂ and ♀ from Amazonas and Mato Grosso, Brazil); T. caraca sp. nov. (♂ and ♀ from south and southeast Brazil); T. urucu sp. nov. (♂ from Amazonas, Brazil); T. coari sp. nov. (♂ and ♀ from Amazonas, Brazil); T. lata sp. nov. (♂ and ♀ from the states of Bahia, Espiríto Santo, Minas Gerais, São Paulo and Santa Catarina, Brazil); T. regiae sp. nov. (♂ and ♀ from Amazonas and Pará, Brazil); T. mutum sp. nov. (♂ and ♀ from Pará and Mato Grosso, Brazil); T. cruzes sp. nov. (♂ and ♀ from Rio de Janeiro, São Paulo and Santa Catarina, Brazil); T. palmares sp. nov. (♂ and ♀ from northeast Brazil); T. una sp. nov. (♂ and ♀ from Bahia, Brazil); T. gigantea sp. nov. (♂ and ♀ from Vaupés, Colômbia and Peru); T. oba sp. nov. (♀ from Bahia, Brazil); T. goeldi sp. nov. (♀ from Pará, Brazil); T. ibiapaba sp. nov. (♂ and ♀ from Ceará, Brazil); T. luctuosa sp. nov. (♀ from Minas Gerais, Brazil) and T. araguaia sp. nov. (♂ and ♀ from Pará, Brazil). Additionally, two species groups are delimited based on genitalic morphology. Distribution maps and an identification key to the males and females of all known species of Tupirinna are also provided.
Published: 2021

29. Zika virus infects human blood mononuclear cells

Author: Vinicius Cotta-de-Almeida, Myrna C. Bonaldo, Dumith Chequer Bou-Habib, Bruno Cister-Alves, Daniela P. Cunha, Zilton Vasconcelos, Carolina V. Messias, Wilson Savino, Daniella Arêas Mendes-da-Cruz, Lidiane M. S. Raphael, and Julia P. Lemos
Subjects: 0301 basic medicine, CD3, CD14, Antigens, CD19, CD8-Positive T-Lymphocytes, Biology, Real-Time Polymerase Chain Reaction, Peripheral blood mononuclear cell, Virus, Monocytes, Zika virus, Flow cytometry, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, Immune system, medicine, Humans, lcsh:RC109-216, 030212 general & internal medicine, Lymphocytes, Cells, Cultured, B-Lymphocytes, medicine.diagnostic_test, Zika Virus Infection, RT-qPCR, biology.organism_classification, Virology, 030104 developmental biology, Infectious Diseases, Leukocytes, Mononuclear, biology.protein, Brazil, CD8, Research Article
Abstract: Background Zika virus (ZIKV) infection gained public health concern after the 2015 outbreak in Brazil, when microcephaly rates increased in babies born from infected mothers. It was demonstrated that ZIKV causes a congenital Zika virus syndrome, including various alterations in the development of the central nervous system. Although the infection of cells from the nervous system has been well documented, less is known in respect of ZIKV ability to infect immune cells. Herein, we investigated if peripheral blood mononuclear cells (PBMCs), freshly-isolated from healthy donors, could be infected by ZIKV. Methods PBMCs from healthy donors were isolated and cultured in medium with ZIKV strain Rio-U1 (MOI = 0.1). Infection was analyzed by RT-qPCR and flow cytometry. Results We detected the ZIKV RNA in PBMCs from all donors by RT-qPCR analysis. The detection of viral antigens by flow cytometry revealed that PBMC from more than 50% the donors were infected by ZIKV, with CD3+CD4+ T cells, CD3−CD19+ B cells and CD3+CD8+ T cells being, respectively, the most frequently infected subpopulations, followed by CD14+ monocytes. Additionally, we observed high variability in PBMC infection rates among different donors, either by numbers or type infected cells. Conclusions These findings raise the hypothesis that PBMCs can act as a reservoir of the virus, which may facilitate viral dissemination to different organs, including immune-privileged sites.
Published: 2019
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30. Morphological and molecular characterization of Stigonema jureiensis sp. nov. (Nostocales, Cyanobacteria) from the Atlantic Rainforest, São Paulo, Brazil

Author: Guilherme Scotta Hentschke, Diego Bonaldo Genuário, Haywood Dail Laughinghouse, Célia Leite Sant'Anna, Janaina Rigonato, Universidade do Porto, Universidade Estadual Paulista (Unesp), Institut de Biologie François Jacob, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), University of Florida / IFAS, and Laboratory of Phycology
Subjects: Nostocales, Microorganism, education.field_of_study, Polyphasic approach, Terrestrial habitats, Phylogenetic tree, biology, Population, Biodiversity, Zoology, Plant Science, Rainforest, 16S ribosomal RNA, biology.organism_classification, Morphological analysis, Tropical environments, Taxonomy (biology), education, Taxonomy
Abstract: Made available in DSpace on 2020-12-12T01:44:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação para a Ciência e a Tecnologia Tropical regions are considered hotspots of biodiversity, and the genus Stigonema has been underexplored in all world, mainly due to the difficulties of culturing. In this study, we sequenced the 16S rRNA gene of a natural Stigonema population from the tropical Atlantic Rainforest (São Paulo, Brazil) and describe S. jureiensis sp. nov. based on phylogenetic and morphological data. For the molecular analysis, the genomic DNA was isolated from aliquots of the population, which were previously manually cleaned under a microscope, using a point–tapered Pasteur pipette to remove other organisms and debris. For morphological analysis, 30 individuals were analyzed and photographed using microscope. Both maximum likelihood and Bayesian phylogenies support S. jureiensis as a new species, and morphological analysis revealed the presence of a special type of mosaic ornate sheaths. Interdisciplinary Centre of Marine and Environmental Research – CIIMAR Universidade do Porto, Av. General Norton de Matos S/N Department of Zoology and Botany Instituto de Biociências Letras e Ciências Exatas (UNESP), R. Cristóvão Colombo, 2265, Jardim Nazareth CEA Centre de Sequençage Genoscope Institut de Biologie François Jacob EMBRAPA Environment Laboratory of Environmental Microbiology Agronomy Department Fort Lauderdale Research and Education Center University of Florida / IFAS, 3205 College Avenue Institute of Botany Laboratory of Phycology, Av. Miguel Stéfano, 3687, Água Funda Department of Zoology and Botany Instituto de Biociências Letras e Ciências Exatas (UNESP), R. Cristóvão Colombo, 2265, Jardim Nazareth FAPESP: 2014/26131–7 CNPq: Multi/04423/2019 Fundação para a Ciência e a Tecnologia: Multi/04423/2019
Published: 2019
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31. Parrotfish functional morphology and bioerosion on SW Atlantic reefs

Author: Rodrigo L. Moura, Ronaldo B. Francini-Filho, Nicole Tiburcio Lellys, Fernando Zaniolo Gibran, and Roberta M. Bonaldo
Subjects: geography, geography.geographical_feature_category, Ecology, Osteology, biology, Functional morphology, Bioerosion, Parrotfish, Aquatic Science, biology.organism_classification, Reef, Ecology, Evolution, Behavior and Systematics
Published: 2019
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32. Compostos voláteis de fungos conidiais sapróbios da Amazônia Meridional no controle in vitro de fitopatógenos

Author: Silmara Aparecida Bonani de Oliveira, Flávia Rodrigues Barbosa, Ednaldo Alves Andrade, Stella Regina Ferrarini, and Solange Maria Bonaldo
Subjects: doenças de plantas, 0303 health sciences, 030306 microbiology, Botany, Plant culture, Plant Science, Biology, SB1-1110, 030308 mycology & parasitology, 03 medical and health sciences, Horticulture, QK1-989, Fungos decompositores, controle biológico
Abstract: RESUMO Estudos sobre compostos orgânicos voláteis (COVs) produzidos por fungos conidiais sapróbios da Amazônia (FCSA) ainda são inexistentes. Assim, verificou-se a produção de COVs de FCSA e, seu potencial no controle de fitopatógenos in vitro. Utilizou-se os FCSA Beltrania rhombica, Brachysporiella sp., Dictyochaeta sp., Gonytrichum sp. e avaliou-se a produção de COVs através da germinação e viabilidade de esporos dos fitopatógenos e, crescimento micelial, conforme metodologias de (2) e (10), com adaptações. Houve redução de germinação de esporos de Colletotrichum musae (63,56%) frente à exposição a B. rhombica, Brachysporiella sp. (86,66%), Dictyochaeta sp. (79,68%) e Gonytrichum sp. (85,71%). Os fitopatógenos C. truncatum, C. musae e Fusarium sp. quando expostos a COVs de Brachysporiella sp., Dictyochaeta sp. e Gonytrichum sp. apresentaram esporos inviáveis após 3 e 7 dias de exposição. Os COVs dos FCSA reduziram o índice de velocidade do crescimento micelial e inibiram o crescimento micelial de Sclerotinia sp. e S. sclerotiorum; bem como redução da produção de escleródios na exposição aos COVs de B. rhombica, B. gayanaa e D. fertilis. Conclui-se que os FCSA estudados apresentam produção de COVs, com potencial para o controle biológico de doenças de plantas.
Published: 2019
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33. Development, characterization and stability of microemulsionated formulations of bacaba, Oenocarpus bacaba oil

Author: Elton Brito Ribeiro, Solange Maria Bonaldo, Dênia Mendes de Sousa Valladão, Rhuan Vínicius de Freitas Esprendor, and M. P. R. Torres
Subjects: Oenocarpus bacaba, native fruit, Science (General), biology, nanotechnology, Chemistry, DPPH, carriers, 04 agricultural and veterinary sciences, 02 engineering and technology, 021001 nanoscience & nanotechnology, biology.organism_classification, microemulsion, 040401 food science, chemistry.chemical_compound, Q1-390, 0404 agricultural biotechnology, Microemulsion, Food science, Amazon forest, 0210 nano-technology, General Agricultural and Biological Sciences, Amazon
Abstract: Advances in nanotechnology, combined with the use of natural products, represent a promissing research field. Brazil is a country of a rich biodiversity, especially in the Amazon forest. Fruits commonly used by local communities, such as bacaba (Oenocarpus bacaba), are potentially important for prospection of industrial applications of natural products. In nanotechnology, microemulsions stand out for providing a modified release to conveyed substances. This work aimed to develop microemulsionated formulations of bacaba oil, characterize them and evaluate their stability. We determined the HLB (hydrophile-lipophilic balance) of bacaba oil for formulation development. Six formulations were selected from pseudoternary diagrams, which indicated the proportions of surfactants, aqueous phase and bacaba oil. The viability of these formulations was evaluated through stability tests. We provided the rheological characterization of the formulations, evaluated their potential antioxidant activity through the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method, and determined the hydrodynamic diameter of the droplets. The microemulsions were stable throughout the test period. Droplet diameter was below 200 nm, and the microemulsions were characterized as newtonian fluids, presenting an increase in antioxidant activity when compared with the diluted oil. Our results confirm the potential of bacaba oil in microemulsionated formulation as a suitable carrier for active compounds.
Published: 2019

34. A novel murine model for arrhythmogenic cardiomyopathy points to a pathogenic role of Wnt signalling and miRNA dysregulation

Author: Mohsin A.F. Khan, Giuseppe Lembo, Paolo Bonaldo, Daniela Carnevale, Alessandra Lorenzon, Alessandra Rampazzo, Patrizia Sabatelli, Claudia Sacchetto, Roberto Doliana, Libero Vitiello, Leon J. De Windt, Roman S. Polishchuk, Giorgia Beffagna, Paola Braghetta, Giulia Poloni, Martina Calore, Emanuela Dazzo, Cardiologie, RS: FSE DMG, RS: CARIM - R2 - Cardiac function and failure, and RS: Carim - H05 Gene regulation
Subjects: 0301 basic medicine, NUCLEAR PLAKOGLOBIN, Physiology, Arrhythmogenic cardiomyopathy, Cardiomyopathy, 030204 cardiovascular system & hematology, medicine.disease_cause, CARDIOMYOCYTES, Pathogenesis, 0302 clinical medicine, Gene Regulatory Networks, INTERCALATED DISKS, Wnt Signaling Pathway, Arrhythmogenic Right Ventricular Dysplasia, Mutation, Desmoglein 2, Arrhythmogenic cardiomyopathy Molecular pathogenesis miRNA, biology, Wnt signaling pathway, PROLIFERATION, Phenotype, LEADS, Codon, Nonsense, Cardiology and Cardiovascular Medicine, STEM-CELLS, RIGHT-VENTRICULAR CARDIOMYOPATHY, Nonsense mutation, Molecular pathogenesis, miRNA, CARDIAC DIFFERENTIATION, Mice, Transgenic, Sudden death, CONTRIBUTES, 03 medical and health sciences, Physiology (medical), medicine, Animals, Genetic Predisposition to Disease, Desmoplakin, Myocardium, Editorials, medicine.disease, Fibrosis, ADIPOGENESIS, Mice, Inbred C57BL, Disease Models, Animal, MicroRNAs, 030104 developmental biology, Gene Expression Regulation, biology.protein, Cancer research, Transcriptome
Abstract: Aims Arrhythmogenic cardiomyopathy (AC) is one of the most common inherited cardiomyopathies, characterized by progressive fibro-fatty replacement in the myocardium. Clinically, AC manifests itself with ventricular arrhythmias, syncope, and sudden death and shows wide inter- and intra-familial variability. Among the causative genes identified so far, those encoding for the desmosomal proteins plakophilin-2 (PKP2), desmoplakin (DSP), and desmoglein-2 (DSG2) are the most commonly mutated. So far, little is known about the molecular mechanism(s) behind such a varied spectrum of phenotypes, although it has been shown that the causative mutations not only lead to structural abnormalities but also affect the miRNA profiling of cardiac tissue. Here, we aimed at studying the pathogenic effects of a nonsense mutation of the desmoglein-2 gene, both at the structural level and in terms of miRNA expression pattern.Methods and results We generated transgenic mice with cardiomyocyte-specific overexpression of a FLAG-tagged human desmoglein-2 harbouring the Q558* nonsense mutation found in an AC patient. The hearts of these mice showed signs of fibrosis, decrease in desmosomal size and number, and reduction of the Wnt/beta-catenin signalling. Genome-wide RNA-Seq performed in Tg-hQ hearts and non-transgenic hearts revealed that 24 miRNAs were dysregulated in transgenic animals. Further bioinformatic analyses for selected miRNAs suggested that miR-217-5p, miR-499-5p, and miR-708-5p might be involved in the pathogenesis of the disease.Conclusion Down-regulation of the canonical Wnt/beta-catenin signalling might be considered a common key event in the AC pathogenesis. We identified the miRNA signature in AC hearts, with miR-708-5p and miR-217-5p being the most up-regulated and miR-499-5p the most down-regulated miRNAs. All of them were predicted to be involved in the regulation of the Wnt/beta-catenin pathway and might reveal the potential pathophysiology mechanisms of AC, as well as be useful as therapeutic targets for the disease.
Published: 2019
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35. Author Correction: Low vector competence in sylvatic mosquitoes limits Zika virus to initiate an enzootic cycle in South America

Author: Erich Loza Telleria, Lidiane Menezes Souza Raphael, Márcia Gonçalves de Castro, Ricardo Lourenço-de-Oliveira, Anielly Ferreira-de-Brito, Myrna C. Bonaldo, Maria Ignez Lima Bersot, and Rosilainy Surubi Fernandes
Subjects: Multidisciplinary, biology, Science, Vector (epidemiology), Medicine, Enzootic, biology.organism_classification, Virology, Competence (human resources), Zika virus
Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Published: 2021
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36. Recovery of Synthetic Zika Virus Based on Rio-U1 Isolate Using a Genetically Stable Two Plasmid System and cDNA Amplification

Author: Ieda Pereira Ribeiro, Marcelo Pelajo-Machado, Lidiane Menezes Souza Raphael, Myrna C. Bonaldo, Pedro Paulo de Abreu Manso, Mônica da Silva Nogueira, Déberli Ruiz Fernandes, Nathália Dias Furtado, Adalgiza da Silva Rocha, Stephanie O. D. da Cruz, Alexandre Dos Santos, Marta Pereira Santos, and Iasmim Silva de Mello
Subjects: Microbiology (medical), Clone (cell biology), lcsh:QR1-502, Virulence, Microbiology, Virus, lcsh:Microbiology, Zika virus, law.invention, 03 medical and health sciences, Plasmid, law, Complementary DNA, Polymerase chain reaction, Original Research, 030304 developmental biology, 0303 health sciences, biology, 030306 microbiology, AG129 mouse infection, infectious clone, cell infection, biology.organism_classification, Virology, Flavivirus, two-plasmid system, cDNA amplification
Abstract: In 2016, the world experienced the unprecedented Zika epidemic. The ZIKV emerged as a major human pathogen due to its association with the impairment of perinatal development and Guillain–Barré syndrome. The occurrence of these severe cases of Zika points to the significance of studies for understanding the molecular determinants of flavivirus pathogenesis. Reverse genetics is a powerful method for studying the replication and determinants of pathogenesis, virulence, and viral attenuation of flaviviruses, facilitating the design of vaccines and therapeutics. However, the main hurdle in the development of infectious clones is the instability of full-length cDNA in Escherichia coli. Here, we described the development of a genetically stable and efficient infectious clone based on the ZIKV Rio-U1 isolated in the 2016 epidemic in Brazil. The employed strategy consisted of cloning the viral cDNA genome into two stable plasmid subclones and obtaining a high-quality cDNA template with increment in DNA mass for in vitro transcription by PCR amplification. The strategy for developing a ZIKV infectious cDNA clone designed in this study was successful, yielding a replicative and efficient clone-derived virus with high similarities with its parental virus, Rio-U1, by comparison of the proliferation capacity in mammal and insect cells. The infection of AG129 immunocompromised mice caused identical mortality rates, with similar disease progression and morbidity in the animals infected with the parental and the cDNA-derived virus. Histopathological analyses of mouse brains infected with the parental and the cDNA-derived viruses revealed a similar pathogenesis degree. We observed meningoencephalitis, cellular pyknosis, and neutrophilic invasion adjacent to the choroid plexus and perivascular cuffs with the presence of neutrophils. The developed infectious clone will be a tool for genetic and functional studies in vitro and in vivo to understand viral infection and pathogenesis better.
Published: 2021
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37. A new species of Trachelopachys Simon, 1897 (Araneae: Trachelidae) from Amazonia

Author: Pantoja, Paulo, Saturnino, Regiane, and Bonaldo, Alexandre B.
Subjects: Male, Corinnidae, Arthropoda, Amazon rainforest, Zoology, Species diversity, Spiders, Biodiversity, Biology, Genus, Arachnida, Animals, Animalia, Araneae, Female, Animal Science and Zoology, Carapace, Animal Distribution, Brazil, Ecology, Evolution, Behavior and Systematics, Taxonomy
Abstract: The genus Trachelopachys Simon, 1897 was erected to accommodate Trachelas sericeus Simon, 1886, a species known from southern South America. Platnick (1975) presented a taxonomic review of the genus and Platnick & Rocha (1995) added a new species and transferred two additional species to the genus, raising the known species diversity of Trachelopachys to 15 species. The genus is distributed in South America, their members are small and colorful, and are distinguished from other trachelines by their darkened carapace, the S-shaped tegular sperm duct and the retrolaterally directed embolus of the male palp (Platnick 1975: fig 12), and the presence of a basal spermathecal lobe in females (Platnick 1975: fig 15). Here we describe the 16th species of the genus, Trachelopachys camarapi sp. nov. (Fig. 1), known from both sexes and recorded in a natural savanna (Fig. 2) at Portel municipality, state of Pará, Brazil.
Published: 2021
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38. SARS-CoV-2 variant N.9 identified in Rio de Janeiro, Brazil

Author: Luana Damasceno, Paola Cristina Resende Silva, Myrna C. Bonaldo, Marta Pereira Santos, Alexandre Dos Santos, Ieda Pereira Ribeiro, Luis Fernando López Tort, Marilda M. Siqueira, Patrícia Brasil, and Lidiane S. R. Menezes
Subjects: Microbiology (medical), Genetics, Lineage (genetic), Phylogenetic tree, Coronavirus disease 2019 (COVID-19), SARS-CoV-2, E484K, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), RC955-962, COVID-19, virus diseases, Biology, Microbiology, QR1-502, Reverse transcription polymerase chain reaction, Phylogenetics, Arctic medicine. Tropical medicine, lineage N.9, Mutation, variants of interest, Rio de Janeiro, Humans, Phylogeny, Brazil, Research Article
Abstract: BACKGROUND The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) B.1.1.33-derived lineage named N.9 was described recently in Brazil and it’s considered a potential variant of interest (VOI) due to the presence of E484K substitution at the receptor-binding domain (RBD) of the Spike (S) protein. OBJECTIVE To describe the first detection of variant N.9 in Rio de Janeiro State. METHODS SARS-CoV-2 N.9 was confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR), wholegenome sequencing and phylogenetic analysis. FINDINGS Here, we report two SARS-CoV-2 N.9 lineage strains in Rio de Janeiro. One of them had only the E484K substitution of the six N.9 lineage-defining mutations. Other three strains pre-defined as N.9 have the same genomic profile. These four strains are grouped within the B.1.1.33 lineage and basal to the N.9 lineage in our phylogenetic analysis, and we call them “N.9- like/B.1.1.33 + E484K”. MAIN CONCLUSIONS The phylogenetic analysis shows four independent introductions of N.9 in the state of Rio de Janeiro in October and December 2020, January and March 2021. SARS-CoV-2 N.9 dissemination in the Rio de Janeiro could have been limited by the emergence and dominance of other variants, mainly by the lineage P.2 VOI Zeta that emerged in the same period and co-circulated with N.9, as observed in the neighboring State of Sao Paulo.
Published: 2021
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39. G Protein-Coupled Estrogen Receptor Immunoreactivity in the Rat Hypothalamus Is Widely Distributed in Neurons, Astrocytes, and Oligodendrocytes, Fluctuates during the Estrous Cycle, and Is Sexually Dimorphic

Author: Paloma Collado, Luis M. Garcia-Segura, Marilena Marraudino, Brigitta Bonaldo, Iciar Garate, Beatriz Carrillo, Ricardo Llorente, Helena Pinos, Elia Campioli, Daniela Grassi, Ministero dell'Istruzione, dell'Università e della Ricerca, Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (España), Instituto de Salud Carlos III, and European Commission
Subjects: Male, medicine.medical_specialty, Lateral hypothalamus, Endocrinology, Diabetes and Metabolism, Wistar, Hypothalamus, Estrous Cycle, 030209 endocrinology & metabolism, Biology, Supraoptic nucleus, Receptors, G-Protein-Coupled, 030218 nuclear medicine & medical imaging, G-Protein-Coupled, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Endocrinology, Arcuate nucleus, Internal medicine, Receptors, Paraventricular nucleus, medicine, Animals, Rats, Wistar, Neurons, Estrous cycle, Sex Characteristics, Endocrine and Autonomic Systems, Ventromedial hypothalamus, Lordosis behavior, Astrocytes, Female, Immunohistochemistry, Oligodendroglia, Rats, nervous system, biology.protein, NeuN, GPER, hormones, hormone substitutes, and hormone antagonists
Abstract: Introduction: The membrane-associated G protein-coupled estrogen receptor 1 (GPER) mediates the regulation by estradiol of arginine-vasopressin immunoreactivity in the supraoptic and paraventricular hypothalamic nuclei of female rats and is involved in the estrogenic control of hypothalamic regulated functions, such as food intake, sexual receptivity, and lordosis behavior. Objective: To assess GPER distribution in the rat hypothalamus. Methods: GPER immunoreactivity was assessed in different anatomical subdivisions of five selected hypothalamic regions of young adult male and cycling female rats: the arcuate nucleus, the lateral hypothalamus, the paraventricular nucleus, the supraoptic nucleus, and the ventromedial hypothalamic nucleus. GPER immunoreactivity was colocalized with NeuN as a marker of mature neurons, GFAP as a marker of astrocytes, and CC1 as a marker of mature oligodendrocytes. Results: GPER immunoreactivity was detected in hypothalamic neurons, astrocytes, and oligodendrocytes. Sex and regional differences and changes during the estrous cycle were detected in the total number of GPER-immunoreactive cells and in the proportion of neurons, astrocytes, and oligodendrocytes that were GPER-immunoreactive. Conclusions: These findings suggest that estrogenic regulation of hypothalamic function through GPER may be different in males and females and may fluctuate during the estrous cycle in females., This study was supported by the Ministero dell’Istruzione, dell’Università e della Ricerca, Italy (MIUR project Dipartimenti di Eccellenza 2018–2022) to the Department of Neuroscience Rita Levi Montalcini, Agencia Estatal de Investigación, Spain (BFU201782754-R), Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable, Instituto de Salud Carlos III, Madrid and Fondos FEDER. The fellowship of M. Marraudino was generously granted by Prof. G.C. Bergui.
Published: 2021

40. Identification of sources of resistance of Passiflora rootstocks to fusariosis in areas with disease outbreaks in Mato Grosso state, Brazil

Author: D. S. Miguel-Wruck, Maurel Behling, Flávio Dessaune Tardin, Valeria de Oliveira Faleiro, Solange Maria Bonaldo, and Givanildo Roncatto
Subjects: 0106 biological sciences, Fusarium, Randomized block design, enxerto, Fusarium oxysporum f. sp.passiflorae, Plant Science, Biology, resistência à fusariose, 01 natural sciences, SB1-1110, Passiflora, Cultivar, fusariosis resistance, Hybrid, Plant culture, food and beverages, 04 agricultural and veterinary sciences, biology.organism_classification, Passiflora sp, grafting, Transplantation, Horticulture, Fusarium oxysporum f. sp. passiflorae, Loam, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Rootstock, Agronomy and Crop Science, 010606 plant biology & botany, Food Science
Abstract: The aim of the study was to identify sources of resistance of Passiflora rootstocks to Fusarium sp. in two experimental areas with histories of disease foci in Mato Grosso state, Brazil. The experiment was carried out from June 2012 to December 2015 and was of randomized block design with eight treatments in which susceptible cultivar P. edulis was grafted onto hybrids CPAC MJ-H-87, CPAC MJ-H-76, CPAC MJ-H-86 and CPAC MJ-H-88, the commercial cultivar and native P. edulis, P. nitida and P. alata. Mortality rates of grafted plants were evaluated up to 640 days after transplantation. All Fusarium sp. isolates were identified as F. oxysporum f. sp. passiflorae. Plant mortality occurred earlier in the clay area than in the area with sandy clay loam. Grafted plants involving P. edulis as rootstocks were highly susceptible to Fusarium sp. with overall mortality rates above 56.25% considering both soil types, while plants with CPAC MJ-H-76 or native P. nitida rootstocks were more resistant with mortality rates below 12.5%. Grafted plants with CPAC MJ-H-86 as rootstock were highly resistant in clay soil but highly susceptible in soil with high proportion of sand. Only grafted plants involving CPAC MJ-H-76 as rootstock showed moderate resistance. Resumo O objetivo do estudo foi identificar fontes de resistência de porta-enxertos de Passiflora ao Fusarium sp. em duas áreas experimentais, com registro de focos da doença em Mato Grosso, Brasil. O experimento foi conduzido de junho de 2012 a dezembro de 2015 e delineado em formato de blocos casualizados com oito tratamentos, nos quais a cultivar suscetível Passiflora edulis (copa) foi enxertada nos híbridos CPAC MJ-H-87, CPAC MJ-H-76, CPAC MJ-H-86 e CPAC MJ-H-88, na própria cultivar comercial e nas espécies nativas , P. edulisP. nitida e P. alata. As taxas de mortalidade das plantas enxertadas foram avaliadas até 640 dias após o transplante. Todos os isolados de Fusarium sp. foram identificados como F. oxysporum f. sp. passiflorae. A mortalidade ocorreu mais cedo na área com solo argiloso do que naquele argilo-arenoso. Plantas enxertadas com P. edulis foram altamente suscetíveis ao Fusarium sp. com taxas de mortalidade superior a 56,25%, considerando ambos os tipos de solo, enquanto plantas enxertadas com CPAC MJ-H-76 ou P. nitida foram mais resistentes com taxas de mortalidade inferiores a 12,5%. Plantas enxertadas com CPAC MJ-H-86 foram altamente resistentes em solo argiloso, mas altamente suscetíveis em solo com alta proporção de areia. Somente plantas enxertadas com CPAC MJ-H-76 mostraram ter resistência moderada.
Published: 2021

41. Collagens and Muscle Diseases: A Focus on Collagen VI

Author: Paolo Bonaldo, Matilde Cescon, Valentina Tonelotto, and Silvia Castagnaro
Subjects: Extracellular matrix, medicine.anatomical_structure, Collagen VI, Protein domain, medicine, Skeletal muscle, Muscle disorder, Biology, Gene, Homeostasis, Function (biology), Cell biology
Abstract: The extracellular matrix is a three-dimensional network providing the proper microenvironment for muscle development and function. Different types of collagens play critical roles in skeletal muscle homeostasis, as revealed by the fact that mutations in distinct collagen genes are linked to inherited muscle disorders, including some forms of myopathies and congenital muscular dystrophies. The following chapter discusses the current knowledge of collagens and their roles in muscle disorders, mostly focusing on collagen VI since it is the collagen type with the major known impact in muscle physiology and whose deficiency is causative for a distinctive group of muscle diseases. Collagen VI is a unique member of the collagen superfamily, displaying distinctive protein domains, supramolecular assembly, and tissue distribution. Moreover, it exerts a broad range of essential functions in the skeletal muscle, and mutations of its genes are linked to congenital muscular dystrophies and congenital myopathies. Mutant and knockout animal models for collagen VI and other collagen types provided a tremendous tool for the mechanistic understanding of human muscle diseases linked to collagens’ defects and, thus, for translational studies. Indeed, the increasing knowledge on the underlying pathomolecular mechanisms and the identification of suitable targets represent invaluable benefits for proper clinical diagnosis and for developing treatments and therapeutic opportunities to counteract the onset and progression of clinical symptoms of such severe and often life-threatening muscle pathologies.
Published: 2021
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42. The genus Syspira Simon, 1895 (Araneae: Miturgidae) from Hispaniola, with the description of four new species

Author: Alexandre B. Bonaldo, Gabriel de los Santos, Alexander Sánchez-Ruiz, and Antonio D. Brescovit
Subjects: Male, Arthropoda, biology, Dionycha, Miturgidae, Holotype, Zoology, Spiders, Biodiversity, biology.organism_classification, Arachnida, Animalia, Araneae, Animals, Female, Animal Science and Zoology, Taxonomy (biology), Endemism, Animal Distribution, Ecology, Evolution, Behavior and Systematics, Taxonomy
Abstract: The species of Syspira Simon from Hispaniola are revised and detailed documentation is provided for all known species from this island. Four new species (Syspira alayoni sp. n., S. armasi sp. n., S. barbacoa sp. n., and S. bryantae sp. n.), and the unknown male of Syspira agujas Brescovit, Sánchez-Ruiz & Bonaldo are described. New distribution records were found for three known species (S. agujas, S. cimitarra Brescovit, Sánchez-Ruiz & Bonaldo and S. medialuna Brescovit, Sánchez-Ruiz & Bonaldo). The Hispaniolan endemic species Teminius monticola (Bryant, 1948) is transferred to Syspira based on the revision of its holotype. A key for Hispaniola Syspira species is provided. These species can be separated into two distinct groups based on features of the male palpal morphology and female genitalia: the cimitarra group including S. cimitarra, S. jimmyi Brescovit, Sánchez-Ruiz & Bonaldo, S. alayoni and S. barbacoa sp. n., and the agujas group including S. agujas, S. medialuna, S. monticola comb. n., S. armasi sp. n. and S. bryantae sp. n.
Published: 2020
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43. Monilinema gen. nov., a homocytous genus (Cyanobacteria, Leptolyngbyaceae) from saline-alkaline lakes of Pantanal wetlands, Brazil

Author: Diego Bonaldo Genuário, Marli Fátima Fiore, Célia Leite Sant'Anna, Marcelo Gomes Marçal Vieira Vaz, and Camila Francieli da Silva Malone
Subjects: 0106 biological sciences, Cyanobacteria, DNA, Bacterial, Plant Science, Aquatic Science, 010603 evolutionary biology, 01 natural sciences, Algae, Phylogenetics, Genus, RNA, Ribosomal, 16S, Botany, DNA, Ribosomal Spacer, Nomenclature, Phylogeny, Phylogenetic tree, biology, 010604 marine biology & hydrobiology, Sequence Analysis, DNA, biology.organism_classification, 16S ribosomal RNA, Lakes, Taxon, Wetlands, Brazil
Abstract: Cyanobacteria morphotypes with simple morphology, mainly thin filamentous homocytous strains, comprise a taxonomically complex group and represent a challenge in systematic studies. However, the polyphasic approach applied nowadays to investigate the cyanobacterial diversity has emerged as a powerful tool to undercover cryptic taxa and to set up a more natural classification system. Yet, studies exploring the cultured diversity of cyanobacteria from extreme tropical environments have paved the way to the discovery of new cyanobacteria taxa, enabling the description of Pantanalinema, Alkalinema, and Cephalothrix as novel genera from saline-alkaline lakes in Pantanal wetlands (Brazil). The present study investigated a set of cyanobacterial strains resembling Leptolyngbya morphotypes by means of its morphology, 16S ribosomal RNA (16S rRNA) phylogeny, and ITS secondary structures. Based on the shape and structure of their trichomes, the low levels of 16S rRNA identity coupled with the distinct phylogenetic position with regard to well establish genera, and their divergent ecological feature, these strains must be accommodated into a novel genus, named as Monilinema gen. nov., described under the provisions of the International Code of Nomenclature for algae, fungi, and plants. Accordingly, based on the singularity of the D1-D1' and V3 helixes, these strains should be considered as a single species, Monilinema alkalinum.
Published: 2020

44. Immune outcomes of Zika virus infection in nonhuman primates

Author: Kyra Headrick, Dawn M. Wesson, Lara A. Doyle-Meyers, Margaret H. Gilbert, Robert V Blair, Nicholas J. Maness, Victoria W. Danner, Elizabeth A. Scheef, Blake Schouest, Matthew J. Ward, Antonito T. Panganiban, Marissa Fahlberg, Myrna C. Bonaldo, and Dawn M. Szeltner
Subjects: Male, 0301 basic medicine, Transcription, Genetic, T-Lymphocytes, T cell, 030106 microbiology, lcsh:Medicine, Adaptive Immunity, Monocytes, Article, Virus, Zika virus, 03 medical and health sciences, Immune system, medicine, Animals, Cytotoxic T cell, lcsh:Science, Multidisciplinary, biology, Zika Virus Infection, lcsh:R, Zika Virus, Viral host response, Viral Load, biology.organism_classification, Macaca mulatta, Virology, Immunity, Humoral, Vaccination, Phenotype, 030104 developmental biology, medicine.anatomical_structure, Viral infection, biology.protein, Infectious diseases, lcsh:Q, Antibody, CD8
Abstract: Although the Zika virus (ZIKV) epidemic is subsiding, immune responses that are important for controlling acute infection have not been definitively characterized. Nonhuman primate (NHP) models were rapidly developed to understand the disease and to test vaccines, and these models have since provided an understanding of the immune responses that correlate with protection during natural infection and vaccination. Here, we infected a small group of male rhesus (Macaca mulatta) and cynomolgus (Macaca fascicularis) macaques with a minimally passaged Brazilian ZIKV isolate and used multicolor flow cytometry and transcriptional profiling to describe early immune patterns following infection. We found evidence of strong innate antiviral responses together with induction of neutralizing antibodies and T cell responses. We also assessed the relative importance of CD8 T cells in controlling infection by carrying out CD8 T cell depletion in an additional two animals of each species. CD8 depletion appeared to dysregulate early antiviral responses and possibly increase viral persistence, but the absence of CD8 T cells ultimately did not impair control of the virus. Together, these data describe immunological trends in two NHP species during acute ZIKV infection, providing an account of early responses that may be important in controlling infection.
Published: 2020
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45. Survey on Non-Human Primates and Mosquitoes Does not Provide Evidences of Spillover/Spillback between the Urban and Sylvatic Cycles of Yellow Fever and Zika Viruses Following Severe Outbreaks in Southeast Brazil

Author: Edmilson Moutinho dos Santos, Taissa Pereira dos Santos, Marcelo Quintela Gomes, Danilo Simonini Teixeira, Maycon Sebastião Alberto Santos Neves, Vanessa de Oliveira Santos, Filipe Vieira Santos de Abreu, Christophe Paupy, Andréa Marques Vieira da Silva, Camilla Bayma Fernandes, Márcia Gonçalves de Castro, Luzia Maria de Oliveira-Pinto, Sara Moutailler, Myrna C. Bonaldo, José Henrique Rezende Linhares, Sheila Maria Barbosa de Lima, Ana Paula Dinis Ano Bom, Lena Yousfi, Emily Hime Miranda, Adriana de Souza Azevedo, Alessandro Pecego Martins Romano, Ricardo Lourenço-de-Oliveira, Ieda Pereira Ribeiro, Monique da Rocha Queiroz Lima, Alexandre Dos Santos, Anielly Ferreira-de-Brito, Monique de Albuquerque Motta, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Evolution des Systèmes Vectoriels (ESV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, and Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: 0301 basic medicine, Primates, Genotype, [SDV]Life Sciences [q-bio], 030231 tropical medicine, lcsh:QR1-502, serology, Aedes aegypti, Genome, Viral, Mosquito Vectors, Biology, Arbovirus, lcsh:Microbiology, Article, law.invention, Disease Outbreaks, 03 medical and health sciences, 0302 clinical medicine, law, Virology, Yellow Fever, parasitic diseases, medicine, ZikV Infection, Animals, Zika Virus Infection, flavivirus, Flavivirus, Yellow fever, PRNT, Outbreak, Zika Virus, medicine.disease, biology.organism_classification, high throughput real time PCR, 3. Good health, 030104 developmental biology, Infectious Diseases, Transmission (mechanics), arboviruses, Sylvatic cycle, Yellow fever virus, Brazil
Abstract: In the last decade, Flaviviruses such as yellow fever (YFV) and Zika (ZIKV) have expanded their transmission areas. These viruses originated in Africa, where they exhibit both sylvatic and interhuman transmission cycles. In Brazil, the risk of YFV urbanization has grown, with the sylvatic transmission approaching the most densely populated metropolis, while concern about ZIKV spillback to a sylvatic cycle has risen. To investigate these health threats, we carried out extensive collections and arbovirus screening of 144 free-living, non-human primates (NHPs) and 5219 mosquitoes before, during, and after ZIKV and YFV outbreaks (2015&ndash, 2018) in southeast Brazil. ZIKV infection was not detected in any NHP collected at any time. In contrast, current and previous YFV infections were detected in NHPs sampled between 2017 and 2018, but not before the onset of the YFV outbreak. Mosquito pools screened by high-throughput PCR were positive for YFV when captured in the wild and during the YFV outbreak, but were negative for 94 other arboviruses, including ZIKV, regardless of the time of collection. In conclusion, there was no evidence of YFV transmission in coastal southeast Brazil before the current outbreak, nor the spread or establishment of an independent sylvatic cycle of ZIKV or urban Aedes aegypti transmission of YFV in the region. In view of the region&rsquo, s receptivity and vulnerability to arbovirus transmission, surveillance of NHPs and mosquitoes should be strengthened and continuous.
Published: 2020
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46. Revision of the spider genus Nyetnops Platnick amp; Lise (Araneae: Caponiidae) with proposition of the new genus Nopsma, from Central and South America

Author: Alexander Sánchez-Ruiz, Alexandre B. Bonaldo, and Antonio D. Brescovit
Subjects: Type species, Spider, biology, Caponiidae, Zoology, Animals, Animal Science and Zoology, Taxonomy (biology), Spiders, biology.organism_classification, Animal Distribution, Ecology, Evolution, Behavior and Systematics
Abstract: The singular spider genus Nyetnops Platnick & Lise is revised and three new species are described from Peru (Nyetnops naylienae n. sp.), Bolivia (Nyetnops lachonta n. sp.) and Brazil (Nyetnops buruti n. sp.). An updated diagnosis is proposed for the type species Nyetnops guarani Platnick & Lise and new records from the Brazilian State of São Paulo are presented for this species. The Ecuadorian species Nyetnops juchuy Dupérré is elected as the type species of a new genus herein proposed (Nopsma n. gen.), which also harbors three other new species from Peru (Nopsma enriquei n. sp.), Colombia (Nopsma florencia n. sp.) and Nicaragua (Nopsma armandoi n. sp.).
Published: 2020

47. ZIKA VIRUS VERTICAL TRANSMISSION IN CHILDREN WITH CONFIRMED ANTENATAL EXPOSURE

Author: Luana Damasceno, Leticia Guida, Genhong Cheng, Suan-Sin Foo, Elizabeth B. Brickley, Stephanie L. Gaw, Denise Cotrim da Cunha, Jae U. Jung, Karin Nielsen-Saines, Maria Elisabeth Lopes Moreira, Myrna C. Bonaldo, Liege Maria Abreu de Carvalho, Jose Paulo Pereira, Zilton Vasconcelos, Ieda Pereira Ribeiro, Claudia Raja Gabaglia, Andrea Zin, Lulan Wang, Irena Tsui, Patrícia Brasil, Mirza Rocha, Weiqiang Chen, James D. Cherry, Tara Kerin, Marcos Vinicius da Silva Pone, Saba Roghiyh Aliyari, Sheila Moura Pone, and Kristina Adachi
Subjects: 0301 basic medicine, General Physics and Astronomy, Physiology, First year of life, Diseases, Urine, Polymerase Chain Reaction, law.invention, Zika virus, 0302 clinical medicine, law, Pregnancy, Medicine, 030212 general & internal medicine, lcsh:Science, reproductive and urinary physiology, Polymerase chain reaction, Pediatric, Multidisciplinary, biology, Obstetrics, Transmission (medicine), Zika Virus Infection, 3. Good health, In utero, Virus Diseases, Infectious diseases, Female, Infection, medicine.medical_specialty, Science, Concordance, Communicable Diseases, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Clinical Research, 030225 pediatrics, Humans, business.industry, General Chemistry, Gold standard (test), Zika Virus, Perinatal Period - Conditions Originating in Perinatal Period, biology.organism_classification, medicine.disease, 030104 developmental biology, Immunoglobulin M, Viral infection, Communicable disease transmission, lcsh:Q, business
Abstract: We report Zika virus (ZIKV) vertical transmission in 130 infants born to PCR+ mothers at the time of the Rio de Janeiro epidemic of 2015–2016. Serum and urine collected from birth through the first year of life were tested by quantitative reverse transcriptase polymerase chain reaction (PCR) and/or IgM Zika MAC-ELISA. Four hundred and seven specimens are evaluated; 161 sera tested by PCR and IgM assays, 85 urines by PCR. Sixty-five percent of children (N = 84) are positive in at least one assay. Of 94 children tested within 3 months of age, 70% are positive. Positivity declines to 33% after 3 months. Five children are PCR+ beyond 200 days of life. Concordance between IgM and PCR results is 52%, sensitivity 65%, specificity 40% (positive PCR results as gold standard). IgM and serum PCR are 61% concordant; serum and urine PCR 55%. Most children (65%) are clinically normal. Equal numbers of children with abnormal findings (29 of 45, 64%) and normal findings (55 of 85, 65%) have positive results, p = 0.98. Earlier maternal trimester of infection is associated with positive results (p = 0.04) but not clinical disease (p = 0.98). ZIKV vertical transmission is frequent but laboratory confirmed infection is not necessarily associated with infant abnormalities., Here, Brasil et al. investigate mother to child Zika virus (ZIKV) transmission rates in a large longitudinal cohort of pregnant ZIKV-positive women with their infants followed from the time of maternal infection through birth and onwards, finding high in utero transmission rates that do not predict clinical outcomes, suggesting follow-up of children with antenatal ZIKV exposure is necessary.
Published: 2020
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48. Structural characterization and polymorphism analysis of the NS2B-NS3 protease from the 2017 Brazilian circulating strain of Yellow Fever virus

Author: R.S. Fernandes, Glaucius Oliva, Nathália Dias Furtado, V.O. Gawriljuk, G.D. Noske, Andre S. Godoy, and Myrna C. Bonaldo
Subjects: Models, Molecular, 0301 basic medicine, Protein Conformation, Nausea, viruses, medicine.medical_treatment, Biophysics, Disease, Viral Nonstructural Proteins, Biology, POLIMORFISMO, Polymorphism, Single Nucleotide, Biochemistry, Virus, 03 medical and health sciences, medicine, Molecular Biology, NS3, Protease, 030102 biochemistry & molecular biology, Serine Endopeptidases, Yellow fever, medicine.disease, biology.organism_classification, Virology, Flavivirus, 030104 developmental biology, Mutagenesis, Site-Directed, Yellow fever virus, medicine.symptom, Headaches, Brazil, RNA Helicases
Abstract: The Yellow Fever virus (YFV) is transmitted by mosquitos and causes an infection with symptoms including fever, headaches and nausea. In 20-50% of the cases, the disease may evolve to a visceral stage, reaching high mortality rates. YFV NS2B-NS3 protease has been identified as an important drug target.Herein, we describe the crystal structure of the NS2B-NS3 protease from the 2017 YFV Brazilian circulating strain using X-ray crystallography. Furthermore, we used a combination of biochemical and biophysical assays to characterize the enzyme and investigate the impact of the polymorphisms observed in different YFV circulating strains.Surprisingly, the crystal structure of YFV protease seems to adopt the closed conformation without the presence of a binding partner. Although D88E and K121R mutants exhibited a lower affinity for the substrate, both revealed to be more processive, resulting in a similar catalytic efficiency in relation to the WT protease. Still, both mutants showed an accentuated decrease in stability when compared with the WT.The crystal structure of YFV NS2B-NS3 in closed conformation might be an important tool for the development of new drugs, as well as understanding the activation mechanism of viral proteases. Biochemical analyses indicate that the NS2B-NS3 protease of the circulating strain of YFV is more stable than previous strains.The YFV NS2B-NS3 protease is the first flaviviral structure described in its closed conformation when in a free form, implying that external factors might induce the activation of the enzyme.
Published: 2020

49. Zika virus targets the human thymic epithelium

Author: Guilherme Loss-Morais, Carolina V. Messias, Alexandra L. Gerber, Mariela Natacha González, Vinicius Cotta-de-Almeida, Elyzabeth Avvad Portari, Lidiane M. S. Raphael, Myrna C. Bonaldo, Luis W. P. Arge, Joseane Biso de Carvalho, Wilson Savino, Ingo Riederer, Daniella Arêas Mendes-da-Cruz, Daniela P. Cunha, Maria Elisabeth Lopes Moreira, Nilma Valéria Caldeira Ferreira, Ana Tereza Ribeiro de Vasconcelos, Zilton Vasconcelos, and Désio Aurélio Farias-de-Oliveira
Subjects: lcsh:Medicine, Thymus Gland, Virus, Article, Epithelium, Zika virus, Downregulation and upregulation, Viral envelope, microRNA, Chlorocebus aethiops, Animals, Humans, Cell adhesion, lcsh:Science, Vero Cells, Multidisciplinary, Thymocytes, biology, Zika Virus Infection, lcsh:R, RNA, Epithelial Cells, Zika Virus, biology.organism_classification, Cell biology, Viral Tropism, Lymphoid tissues, Viral infection, Tissue tropism, lcsh:Q
Abstract: Previous work showed that the thymus can be infected by RNA viruses as HIV and HTLV-1. We thus hypothesized that the thymus might also be infected by the Zika virus (ZIKV). Herein we provide compelling evidence that ZIKV targets human thymic epithelial cells (TEC) in vivo and in vitro. ZIKV-infection enhances keratinization of TEC, with a decrease in proliferation and increase in cell death. Moreover, ZIKV modulates a high amount of coding RNAs with upregulation of genes related to cell adhesion and migration, as well as non-coding genes including miRNAs, circRNAs and lncRNAs. Moreover, we observed enhanced attachment of lymphoblastic T-cells to infected TEC, as well as virus transfer to those cells. Lastly, alterations in thymuses from babies congenitally infected were seen, with the presence of viral envelope protein in TEC. Taken together, our data reveals that the thymus, particularly the thymic epithelium, is a target for the ZIKV with changes in the expression of molecules that are relevant for interactions with developing thymocytes.
Published: 2020

50. Bioproducts and Homeopathy in Lasiodiplodia Rot (Lasiodiplodia sp.) Control and Development of Passion Fruit Seedlings

Author: Juliana da Silva Agostini, Daniela Ribeiro, Márcio Roggia Zanuzo, Rogelho Alexandre Trento, Carmen Wobeto, and Solange Maria Bonaldo
Subjects: Passiflora, Transplantation, Lasiodiplodia, Horticulture, biology, Bioproducts, fungi, food and beverages, Bacillus subtilis, Propolis, biology.organism_classification, Completely randomized design, Pichia
Abstract: The aim of this study was to evaluate the effects of homeopathic medicine Calcarea carbonica 12CH and bioproducts: filtrates of saprobic fungi from Amazon, green propolis nosode 06CH, filtrate of Pichia sp., green propolis extract, Bacillus subtilis ; to control Lasiodiplodia ( Lasiodiplodia sp.) and development of passion fruit seedlings ( Passiflora edulis Sims f. flavicarpa Degener). The experiment was carried out in a completely randomized design with 10 treatments in a parcel divided in time. Each plot consisted of 5 plants for analysis. Data were subjected to analysis of variance and when significant compared by Scott-Knott test (p> 0.05). Variables related to plant growth and the area under the disease progress curve (AUDPC) were evaluated. Assessments were performed at 67, 74 and 82 days after transplantation. There was no reduction in AUDPC for treatments compared to control. As for plant height (cm), the best treatments were Pichia sp. and Gonytrichum sp. with an increase in relation to control of 29.70% and 18.24%, respectively, and increased leaf area (cm 2 ) by 27.42% and 19.85%, respectively. B. subtilis , Pichia sp. and Gonytrichum sp., increased total biomass by 42.31%, 32.82% and 21.44%, respectively. The filtrates application from Pichia sp., Filtrate from Gonytrichum sp., C. carbonica 12CH and B. subtilis provided better performance in the development of passion fruit plants with an increase in the main morphological attributes. The results show that the use of bio products improves the development of seedlings, especially the use of yeast and saprophytic fungi by controlling the biochemical and physiological processes of plants during development.
Published: 2022
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