1. Organization of the mitochondrial genome in the dinoflafellate Amphidinium carterae
- Author
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R. Ellen R. Nisbet, Adrian C. Barbrook, Edmund A. Nash, Christopher J. Howe, Kaj Bernhardt, Rachel K. Edwards-Stuart, Nash, Edmund, Barbrook, Adrian, Edwards-Stuart, R, Bernhart, K, Howe, Christopher, and Nisbet, Ellen
- Subjects
Genetics ,Mitochondrial DNA ,Base Sequence ,ved/biology ,Inverted repeat ,Molecular Sequence Data ,ved/biology.organism_classification_rank.species ,Genomics ,Sequence Analysis, DNA ,Biology ,Genome ,Noncoding DNA ,Stop codon ,Evolution, Molecular ,Genes, Mitochondrial ,Amphidinium carterae ,Transfer RNA ,Dinoflagellida ,Animals ,RNA Editing ,Molecular Biology ,Gene ,Ecology, Evolution, Behavior and Systematics - Abstract
We have characterized the mitochondrial genome of the dinoflagellate Amphidinium carterae. It contains just 3 identifiable protein-coding genes: cox1, cox3, and cob. No evidence for rRNA or tRNA genes was found. Expressed sequence tags (EST) sequences for the 3 genes suggest that RNA editing occurs in 2 cases removing an in-frame stop codon. Two of the transcripts (cob and cox1) lack a stop codon at the end of the gene. The genome contains a large amount of noncoding DNA including many fragmented copies of all the 3 genes and large numbers of inverted repeats. The genome, which contains about 70% AT, has undergone extensive recombination, possibly due to the inverted repeats. The highly reduced mitochondrial gene content supports the relationship of the dinoflagellates and apicomplexa as sister groups.
- Published
- 2007