1. miRNA Stability in Frozen Plasma Samples
- Author
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Luca Deiana, Sara Pasella, Baingio Porcu, Angela Baralla, Marta Deiana, Ciriaco Carru, Giuseppe Fanciulli, Annalisa Oggiano, Andrea Mannu, Mario Pescatori, Silvia Dei Giudici, Francesca Balzano, and Angelo Zinellu
- Subjects
Adult ,RNA Stability ,Pharmaceutical Science ,Repressor ,Biology ,Article ,Cryopreservation ,Analytical Chemistry ,lcsh:QD241-441 ,lcsh:Organic chemistry ,Drug Discovery ,microRNA ,sequences AU or UA ,Humans ,Physical and Theoretical Chemistry ,Gene ,miRNA ,Gene Expression Profiling ,Organic Chemistry ,Half-life ,Middle Aged ,stability ,Molecular biology ,Healthy Volunteers ,Gene expression profiling ,MicroRNAs ,Biochemistry ,Blood Preservation ,Chemistry (miscellaneous) ,Molecular Medicine ,Fresh frozen plasma ,long freezing times ,Half-Life - Abstract
MicroRNAs (miRNAs) represent a family of small non-coding ribonucleic acids that post-transcriptionally inhibits the expression of their target messenger RNAs (mRNAs), thereby acting as general gene repressors. In this study we examined the relative quantity and stability of miRNA subjected to a long period of freezing, we compared the stability of eight miRNAs in the plasma of five human healthy controls before freezing and after six and 12 months of storage at −80 °C. In addition, we examined the plasma frozen for 14 years and the amount of miRNA still available. Using a Life Technologies protocol to amplify and quantify plasma miRNAs from EDTA (Ethylene Diamine Tetraacetic Acid)-treated blood, we analyzed the stability of eight miRNAs, (miR-125b-5p, miR-425-5p, miR-200b-5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-126-3p, and miR-21-5p). The miRNAs analyzed showed a high stability and long frozen half-life.
- Published
- 2015
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