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The jellyfish venom stored in nematocysts contains highly toxic compounds comprising of polypeptides, enzymes and other proteins, which form their chemical defence armoury against predators. We have characterized the proteome of crude venom extract from three bloom-forming scyphozoan jellyfish along the south-west coast of India, Chrysaora caliparea, Cyanea nozakii and Lychnorhiza malayensis using a Quadrupole-Time of Flight (Q/TOF) mass spectrometry analysis. The most abundant toxin identified from Chrysaora caliparea and Lychnorhiza malayensis is similar to the pore-forming toxins and metalloproteinases. A protective antioxidant enzyme called peroxiredoxin was found abundantly in Cyanea nozakii. Metalloproteinase identified from the C. caliparea shows similarity with the venom of pit viper (Bothrops pauloensis), while that of L. malayensis was similar to the venom of snakes such as the Bothrops insularis and Bothrops asper. Kininogen-1 is a secreted protein, identified for the first time from the jellyfish L. malayensis. The proteome analysis of Cyanea nozakii, Chrysaora caliparea and Lychnorhiza malayensis contained 20, 12, 8 unique proteins, respectively. Our study characterized the proteome map of crude venom extract from L. malayensis and C. caliparea for the first time, and the venom profile is compared with published information elsewhere. Proteomic data from this study has been made available in the public domain.

Near reef abundance and coast–offshore distribution of tuna larvae around Minicoy Island were studied based on three cruises carried out on board FORV Sagar Sampada during 2014–15. The samples were...

This paper applies reflectance spectroscopy and Advanced Space borne thermal emission reflection radiometer (ASTER) data processing to delineate dolomite-hosted rock phosphate around Udaipur City, Western India, using visible-near infrared (VNIR) and short wave infrared (SWIR) bands of the ASTER sensor. A number of ASTER image spectra and field spectra (resampled to ASTER bands) of pure dolomite and rock phosphate are compared and found to be consistent in terms of the presence of absorption minima at 2.3 µm for dolomite and the absence of the same feature in the spectra of the rock-phosphate. Before attempting spectral mapping, ASTER SWIR band data are clipped using the spatial extent of host rock; which has been prepared from the reference geological map and consequently updated using VNIR-SWIR derived image enhanced products at selected part of the study areas. Two sub-pixel spectral methods namely adaptive coherence estimator (ACE) and matched filtering (MF) methods are applied to ASTER SWIR bands to delineate rock-phosphate-rich zones within dolomite. The output images of the above algorithms are integrated in a principal component (PC) image to delineate phosphate pixels. A principal component; which has similar sign of Eigen vector loading for ACE and MF abundance image is used to delineate rock phosphate within dolomite. The results are validated in the field using a qualitative colorimetric method and also by comparing image spectra of spectrally anomalous zone with the ASTER resampled laboratory spectra of rock phosphate samples. A number of sporadic but important patches of phosphate rich zones in addition to the main surface exposures of the Jhamar Kotra Mine are confirmed in the field. The proposed method is a novel application of spectral mapping of phosphate deposits in dolomitic and carbonatic rocks and present approach of ASTER data processing can be used to delineate phosphate rich zones occurring within similar geological provinces in any parts of the world.

The variations in the protein profile of aortic-valvular (AVE) and endocardial endothelial (EE) cells are currently unknown. The current study’s objective is to identify differentially expressed proteins and associated pathways in both the endothelial cells. We used endothelial cells isolated from the porcine (Sus scrofa) aortic valve and endocardium for the profiling of proteins. Label-free proteomics was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Our proteomics analysis revealed that 29 proteins were highly expressed, and 25 proteins were less expressed in the valve than the endocardial endothelium. The cell surface markers, such as CD63, ICAM1, PECAM1, PROCR, and TFRC, were highly expressed in EE. In contrast, CD44 was highly expressed in AVE. The pathway analysis showed that metabolic process-related proteins and extracellular matrix-related proteins were enriched in valves. Differential enrichment of signaling pathways was observed in the endocardium. The hemostasis function-related proteins were increased in both endothelial cells. The proteins and pathways enriched in aortic-valvular and endocardial endothelial cells revealed the distinct phenotype of these two closely related cells.

This paper reports a new record of Zenion hololepis (Goode & Bean, 1896) from Indian waters. The species was collected during an exploratory deep-sea fishery survey onboard FORV Sagar Sampada off the Andaman Islands at a depth of 314 m using a High-Speed Demersal Trawl-Crustacean Version (HSDT-CV) on 25 November 2017. Keywords: Andaman Sea, Deep-Sea, Dwarf dory, Exploratory survey

Leptin is known to regulate pathways of energy metabolism, reproduction, and control appetite. Whether plasma leptin levels reflect changes in metabolites of these pathways is unknown. We aimed to find whether there is an association between leptin levels and levels of metabolites of energy and hormone metabolism. We performed an untargeted metabolomics analysis of plasma from 110 healthy adults (men: women = 1:1; aged 18–40 years), using liquid chromatography-tandem mass spectrometry. Blood samples were collected from all the study subjects in the fasting state. Clinical features and markers of obesity and Type 2 diabetes mellitus (T2DM) were assessed in all. The association between levels of metabolites and clinical and biochemical parameters was identified using the multivariable-adjusted linear regression model and PLS-DA analysis. The leptin level was found to have a significant association with a substantial number of metabolites in women and men. Leptin level was positively associated with glycocholic acid and arachidic acid, metabolites related to energy metabolisms, pregnanediol-3-glucuronide, a metabolite of progesterone metabolism, and quercetin 3′-sulfate, a diet-derived metabolite. Leptin level was negatively associated with ponasteroside A and barringtogenol C levels. Leptin level was positively correlated with adiponectin and negatively with total calorie intake and levels of triglyceride and very-low-density lipoprotein. Leptin levels were associated with lipid and sex hormone metabolism in women, while metabolites involved in amino acid metabolism were correlated to leptin in men. Our study indicates that leptin level reflects metabolome alterations and hence could be a useful marker to detect early changes in energy and hormone metabolisms.

The distribution of Kogiid whales from the Indian Ocean waters, especially Indian waters are poorly known. It is extremely difficult to differentiate the two species (Kogia sima and K. breviceps) based on their morphological characteristics alone. Our study presents the first confirmed record of dwarf sperm whale Kogia sima from the Indian waters using morphological examinations, skull morphology and molecular identification. The study is based on the stranded animal found in the west coast of Agatti Island in Lakshadweep Archipelago belongs to the Indian EEZ. K. sima showed intra-species genetic variability which confirms the isolation of the species in oceans as the tropical nature of the species restricts its movement.

Length weight relationships are presented for five deep‐sea anglerfishes collected from Andaman and Nicobar waters during April 2016 using a 38 m high speed demersal trawl II (HSDT II, crustacean version, codend mesh size 40 mm). The b values ranged from 1.80 to 2.76 and the coefficient of variation (r²) ranged from 0.82 to 0.97.

Summary The objective of this study was to estimate a prey body size from the hard parts (e.g. otoliths) of a fish species frequently found in the guts of predators. Length–weight relationships between otolith size (length, height, weight and aspect ratio) and fish size (total length and weight) were determined for four fish species captured in the Arabian Sea by bottom trawl (2015 survey on-board FORV Sagar Sampada, 200–300 m depth), off the west coast of India: Psenopsis cyanea, Pterygotrigla hemisticta, Bembrops caudimacula and Hoplostethus rubellopterus. No significant differences were noted between the size of the left and right otoliths (t test) in any of the four species. The length–weight relationship of the otolith in all four species showed a negative allometric growth pattern (t test, p

8 pages, 4 figures, 3 tables, 1 appendix.-- This is CMLRE contribution number 73, It is necessary to understand the resource partitioning among species, to describe the functioning of deep sea marine ecosystems. Functional morphology is an effective approach to understand and compare taxonomic units with different phenotypic characteristics related to swimming and foraging. In this study, our main objective was to delineate the functional traits with a view to determine the discrimination level and degree of functional niche overlap among seven common species inhabiting deep-sea waters in south-eastern Arabian Sea. Results indicated significant differences in the functional traits between species providing a low functional niche overlap. Although, the ecological and biological information of fishes were scarce, we demonstrated that functional analyses are effective to extrapolate the prey preferences, sizes and detection and propulsion efficiencies for their feeding and swimming strategies. Our study supports the hypothesis that in some environments with a limitation of resources, species coexisting is due to high resource partitioning. The present work is a first attempt to compare the morpho-functional characteristics and niche partitioning in the deep-sea fish assemblages from the Indian waters., The financial and logistical support from the CMLRE (MoES), is appreciatively acknowledged

Length‐weight relationships (LWR) for eight deep‐sea fishes belonging to six families collected from the south west coast of India are presented. Specimens were caught by deep‐sea shrimp trawls with mesh size 24 mm during 2011 and 2013. The b values ranged from 2.13 to 3.71 and the coefficient of determination (r2) ranged from 0.84 to 0.98. A new standard length maximum was recorded for Neoscopelus microchir. The b values estimated during the present study were compared with the values estimated using models based on Bayesian approach deposited in Fish Base.

Sustainable development is challenged by the high inputs of agrochemicals in intensified agriculture system. The natural roles of microorganisms in maintaining soil fertility and biocontrol of plant pathogens represent a promising sustainable solution to improve agricultural production. Microbiomes associated with plant complex can promote plant growth, support plant nutrition, and antagonize plant pathogens. The integration of beneficial plant-microbe and microbiome into the widespread commercial use of the plant beneficial microorganisms is required for sustainable development. This chapter focuses on (i) beneficial fungi and beneficial bacteria in rhizosphere; (ii) the function of beneficial microbes for cropping system; (iii) novel approaches for microorganism identification, including functional gene approach, microarray,and proteomic methods.

A new species of argentinid fish, Glossanodon macrocephalus Bineesh & Endo, from the Arabian Sea, off the southwestern coast of India, is described on the basis of four specimens (129–164 mm SL). The new species clearly differs from its congeners in the following combination of characters: dorsal-fin rays 10–11; pectoral-fin rays 21–23; anal-fin rays 12–13; pelvic-fin rays 11–12; branchiostegal rays 5; gill rakers on first arch 30–36; vertebrae 47–48; a patch of small conical teeth on middle of lower jaws; predorsal 49–51% SL; prepectoral 36–39% SL; prepelvic 57–58% SL; distance from pelvic to anal-fin origin 26–28% SL; head length 35–39% SL; eye diameter 22–26% HL; snout length 28–30% HL; location of anus immediately anterior to anal-fin origin; broad longitudinal black stripe above lateral line; dark spot on the base to half of pectoral fin; and no teeth on tongue. The new species is a benthopelagic dweller on muddy bottoms in depths of around 300–600 m.

We examined the hitherto unexplored role of mitochondrial transporters and iron metabolism in advancing metabolic and mitochondrial dysfunction in the heart during long term pressure overload. We also investigated the link between mitochondrial dysfunction and fluctuation in mitochondrial transporters associated with pressure overload cardiac hypertrophy. Left ventricular hypertrophy (LVH) was induced in 3-month-old male Wistar rats by constriction of the aorta using titanium clips. After sacrifice at the end of 6 and 15 months after constriction, tissues from the left ventricle (LV) from all animals were collected for histology, biochemical studies, proteomic and metabolic profiling, and gene and protein expression studies. LV tissues from rats with LVH had a significant decrease in the expression of ABCB7 and mitochondrial oxidative phosphorylation (mt-OXPHOS) enzymes, an increased level of lipid metabolites, decrease in the level of intermediate metabolites of pentose phosphate pathway and elevated levels of cytoplasmic and mitochondrial iron, reactive oxygen species (ROS) and autophagy-related proteins. Knockdown of ABCB7 in H9C2 cells and stimulation with angiotensin II resulted in increased ROS levels, ferritin, and transferrin receptor expression and iron overload in both mitochondria and cytoplasm. A decrease in mRNA and protein levels of mt-OXPHOS specific enzymes, mt-dynamics and autophagy clearance and activation of IGF-1 signaling were also seen in these cells. ABCB7 overexpression rescued all these changes. ABCB7 was found to interact with mitochondrial complexes IV and V. We conclude that in chronic pressure overload, ABCB7 deficiency results in iron overload and mitochondrial dysfunction, contributing to heart failure.

The SRY gene is considered as the key player in the male sexual differentiation and developmental pathway. SRY gene mutations account for ~15% of 46,XY disorders of sexual development patients, and majority of them resides within the HMG domain of the protein. In this study, we report a novel missense mutation within the HMG domain of SRY gene, and an A-to-T transition causes E89V amino acid substitution in a 15-year-old female patient with 46,XY karyotype and complete gonadal dysgenesis. Moreover, three-dimensional analysis of protein-DNA complex showed that the replacement of highly hydrophilic glutamic acid residue with a hydrophobic residue like valine would have an impact on the structure of protein. In conclusion, we identified a novel SRY mutation in a 46,XY female patient with complete gonadal dysgenesis, and based on the protein modelling, we propose that the identified mutation could impair normal function of the SRY protein.

This article reviews the importance of bait, bait loss, and depredation in longline fishery operations worldwide. In general, fish bait is preferred over squid due to reduced sea turtle and elasmobranch bycatch. However, there are many reports which have indicated high shark catch and deep hooking when using fish as bait. High and low hooking rates for blue shark have been reported from different fishing areas when using mackerel as bait, indicating the need for further studies on selection of appropriate baits. Conflicting results from many part of the world on the catching efficiency of different bait species on target and nontarget species indicate the need to consider area, species specific and cross taxa effect of various combinations of baits and hooks, before their adoption in commercial fishing. Baiting pattern has also been reported to affect the catch rates significantly. The review has revealed that bait loss and depredation on the hooked fish cause considerable damage to the fishery an...

Owstonia kamoharai Endo, Liao and Matsuura, 2015 is reported for the first time from the northeastern Indian Ocean. Two specimens measuring 320 mm and 250 mm (Standard Length) were collected during the exploratory fishery surveys by Fishery Oceanographic Research Vessel Sagar Sampada in Andaman and Nicobar waters, India during April 2016 at a depth of 411 m. A brief description of the specimens collected, comparisons and previous distributional records were provided.

Summary Bycatch from the experimental longline operations in the Lakshadweep Sea were studied. The experiments were conducted on converted Pablo boats, originally used for pole and line fishing operations, to capture skipjack tuna in the Lakshadweep Islands. The overall bycatch rate was very high, with a mean hooking rate of 8.05/1000 hooks compared to the targeted tuna catch (1.75/1000 hooks). Bycatch contributed 82.4% of the catch in comparison to the tuna (17.6%) Thunnus albacares, in the longline operations. Silky shark (Carcharhinus falciformes) with 89.9% was the dominant shark species followed by C. amblyrhynchos, Galeocerdo cuvier, Alopias pelagicus, Negapriion acutedens and Sphyrna lewinii with 4.7, 2.7, 1.4, 0.7 and 0.7%, respectively. Sharks contributed to 74.1% of the catch, followed by 15.7% sailfishes and 10.2% miscellaneous fishes. Higher bycatch rates were evident during evening hours compared to mornings, but the results were not significant statistically. Studies on the effects of depth on the overall fishing performance and species selectivity failed to establish any significant relationship at a depth range of 35–100 m. Soaking time had a significant effect on bycatch rates. The hooking rate of sharks declined with an increase in soaking time. Considering the high shark bycatch in the fishery, an accurate monitoring of the longline fish catches in the Lakshadweep waters is an important step towards ensuring the sustainability of other populations, especially sharks.

Summary Length–weight relationships for six deep-sea fish species inhabiting the shelf regions of the western Bay of Bengal and the waters of Andaman and Nicobar of India are presented. Samples were collected using high-speed demersal trawl and expo demersal trawl nets at depths of 31–514 m in August and September 2010. The b values in the analyses ranged from 3.05 to 3.31, showing a mean and median value of 3.21 (SE ± 0.039, SD ± 0.097) and 3.2, respectively. Comparisons of b values with earlier estimations confirm the presence of spatial and temporal variations in the length–weight relations among the species. Coefficient of determination scores ranged from 0.94 to 0.97, indicating robustness of the samples analysed. This study provides the first estimates of length–weight relationships for four of the deep-sea fishes, enriching the understanding of the growth patterns and population dynamics of these less-studied deep-sea fishery resources in Indian Ocean waters.

Endocardial endothelium, which lines the chambers of the heart, is distinct in its origin, structure, and function. Characterization studies using genomics and proteomics have reported molecular signatures supporting the structural and functional heterogeneity of various endothelial cells. However, though functionally very important, no studies at protein level have been conducted so far characterizing endocardial endothelium. In this study, we used endothelial cells from pig heart to investigate if endocardial endothelial cells are distinct at the proteome level. Using a high-throughput liquid chromatography-tandem mass spectrometry for proteome profiling and expression, we identified sets of proteins that belong to specific biological processes and metabolic pathways in endocardial endothelial cells supporting its specific structural and functional roles. The study also identified several transcription factors and cell surface markers, which may have roles in the specificity of endocardial endothelium. The detection of sets proteins preferentially expressed in endocardial endothelium offers new insights into its role in the regulation of cardiac function. Data are made available through ProteomeXchange with identifier PXD009194.

Bembrops caudimacula dominates the deep-sea trawl catch in the exploratory surveys and as bycatch in the commercial trawling targeting deep-sea shrimps, in the Indian Exclusive Economic Zone. The intra-species variability in the bathy-demersal fish Bembrops caudimacula collected from two different ecosystems in the Indian Ocean were studied using otolith morphometric analysis. Five otolith size parameters (ferret length, ferret width, area, perimeter and weight), and five shape indices (roundness, rectangularity, ellipticity, form factor and circularity) were taken from forty-seven otoliths collected from two regions. Principal component analysis (PCA) and non-parametric PERMANOVA successfully differentiate the fishes from two regions based on the otolith morphometry. PCA indicated that ellipticity and otolith weight were the major factor responsible for the variation. The study indicated that otoliths of the fishes collected from Andaman Sea (part of Bay of Bengal) were more elliptic and massy compared to those collected from the Arabian Sea. This is the first work on the differentiation of B. caudimacula population from these two unique ecosystems, using otolith morphometry which is highly imperative to understand its population structure. Our study confirmed the suitability of otolith morphometric studies as an easiest and inexpensive method for differentiating the fish populations.

Adolescence is a crucial period where one tends to identify who they are as an individual. However, as a teenager is struggling to find his/her place in this world, it is also a time where they are prone to engaging in risk behaviors, which tend to have an extreme psychological impact. The objective was to explore the experiences of an adolescent who engages in risk behaviors and to understand their level of personal fables. The study was a qualitative design with content analysis with semi-structured interviews of ten male adolescents aged 16–18 years. The major findings of the study indicated that adolescent's pattern of thinking revolves around the fact that they are invincible and invulnerable. Furthermore, adolescents are aware of the risks they are putting themselves through and how in the process they are hurting others. The implications of the study are to conduct more life skill programs in schools; greater awareness has to be created on the impact and harmful effects of such behaviors.

High-frequencyin vitro flowering is reported here fromin vitro regenerated shoots ofin vitro-raised seedlings of rare and endemicCeropegia lawii, Ceropegia maccannii, Ceropegia oculata, andCeropegia sahyadrica, as well as the widely distributedCeropegia bulbosa var.bulbosa andCeropegia hirsuta. In our first set of experiments, the MS medium contained 87 mM sucrose and was supplemented with varying concentrations of BAP (4.4 to 26.6 µM). For the second set of trials, varying concentrations of sucrose (87 to 233 mM) were tested in MS media containing a constant 4.4 p.M BAP. Sub-cultured apical as well as axillary buds flowered with similar frequencies after 30 d of incubation. For all six species, the highest percentage of flowering shoots was obtained with either 26.6 µM BAP or 175 mM sucrose. Although smaller in size, theirin vitro flowers were morphologically comparable within wVo-derived flowers. Variations among species were noted for the number of flower buds per shoot and the percentage of flower formation. Because all six species showed similar responses in both experiments, we can suggest that this protocol is applicable across the wide range ofCeropegia species.

The Grey Reef Shark Carcharhinus amblyrhynchos is a moderately sized and fairly stocky species widely distributed in the Indian and Pacific Oceans from Easter Island and as far west as South Africa. In this paper, the first record of Grey reef shark (single specimen female 126cm in Total Length (TL)), caught from the Lakshadweep Sea (10o47 N and 72o09 E) by drift long line operation at 40m depth is reported.

The pelagic thresher shark Alopias pelagicus is a large, wide-ranging Indo-Pacific Ocean pelagic shark. In this paper, the first record of the pelagic thresher shark, caught from the Lakshadweep Sea is reported. The shark 275 cm in total length was caught by drift longline operation at depth of 60 m in the Lakshadweep Sea (10°52N latitude 72°13E longitude).

A protocol has been developed for somatic embryogenesis and plant regeneration of sisal (Agave sisalana Perr. ex. Engelm). Embryogenic callus cultures were initiated from young shoots raised in vitro from the stem portion of the bulbil on medium supplemented with 1-2 mg l(-1) kinetin (KN) and 0.2-0.5 mg l(-1) alpha-naphthaleneacetic acid plus KN or 1-1.5 mg l(-1 )benzylaminopurine (BAP) or 0.25-0.5 mg l(-1 )2,4-dichlorophenoxyacetic acid plus BAP or 0.5-1.0 mg l(-1) KN. Embryos at various developmental stages (globular-, heart- or torpedo-shaped) produced mature and germinating embryos on being transferred to a new medium containing 0-0.25 mg l(-1 )KN. After 28 days, a maximum of 76% germinated embryos was obtained on a medium supplemented with 0.1 mg l(-1) KN. The capacity for embryogenesis remained constant in the callus upon subculturing on the same medium for more than 48 months. Histological observations showed a distinct multicellular origin for most of the somatic embryos as they developed from epidermal, sub-epidermal and inside callus cells, while a few of them originated from a superficial callus cell. Plantlets regenerated from embryos were transferred to the field where their survival rate was 100%.

An abscess in a long-standing goitre is usually life threatening. A patient with a large goitre was lost to follow-up for about 10 years and presented with a large thyroid abscess causing acute respiratory obstruction. The abscess caused erosion of the tracheal wall and perforation of the esophagus. Clostridium septicum was the causative organism, which is almost always associated with cancer of the colon. This led to the detection of colonic malignancy in this patient on further investigations. We describe the complications of a thyroid abscess and discuss the pathophysiology and treatment.