1. Aging, inflammation and DNA damage in the somatic testicular niche with idiopathic germ cell aplasia
- Author
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Massimo Alfano, Andrea Salonia, Giovanni Tonon, Silvia Gregori, Alessandra Agresti, Francesca Giannese, Anna Sofia Tascini, Filippo Pederzoli, Jose Manuel Garcia-Manteiga, Giada Amodio, Francesco Montorsi, Manuela Nebuloni, Irene Locatelli, Alfano, M., Tascini, A. S., Pederzoli, F., Locatelli, I., Nebuloni, M., Giannese, F., Garcia-Manteiga, J. M., Tonon, G., Amodio, G., Gregori, S., Agresti, A., Montorsi, F., and Salonia, A.
- Subjects
Male ,Senescence ,Aging ,congenital, hereditary, and neonatal diseases and abnormalities ,endocrine system ,Somatic cell ,Urology ,Science ,General Physics and Astronomy ,Inflammation ,Cell Communication ,Biology ,Article ,General Biochemistry, Genetics and Molecular Biology ,hemic and lymphatic diseases ,Testis ,medicine ,Humans ,Spermatogenesis ,Multidisciplinary ,Gene Expression Profiling ,Leydig Cells ,RNA sequencing ,General Chemistry ,Aplasia ,medicine.disease ,Phenotype ,Spermatogonia ,Gene expression profiling ,stomatognathic diseases ,Germ Cells ,Mechanisms of disease ,medicine.anatomical_structure ,Infertility ,Cancer research ,Chemokines ,medicine.symptom ,Transcriptome ,Genomic imprinting ,Sequence Alignment ,Germ cell ,DNA Damage - Abstract
Molecular mechanisms associated with human germ cell aplasia in infertile men remain undefined. Here we perform single-cell transcriptome profiling to highlight differentially expressed genes and pathways in each somatic cell type in testes of men with idiopathic germ cell aplasia. We identify immaturity of Leydig cells, chronic tissue inflammation, fibrosis, and senescence phenotype of the somatic cells, as well markers of chronic inflammation in the blood. We find that deregulated expression of parentally imprinted genes in myoid and immature Leydig cells, with relevant changes in the ratio of Lamin A/C transcripts and an active DNA damage response in Leydig and peritubular myoid cells are also indicative of senescence of the testicular niche. This study offers molecular insights into the pathogenesis of idiopathic germ cell aplasia., Molecular mechanisms associated with human germ cell aplasia in infertile men remain undefined. Here the authors perform single-cell transcriptome profiling to highlight differentially expressed genes and pathways in each somatic cell type in testes of men with idiopathic germ cell aplasia.
- Published
- 2021