1. Molecular Detection of Drug-Resistance Genes of blaOXA-23-blaOXA-51 and mcr-1 in Clinical Isolates of Pseudomonas aeruginosa
- Author
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Sirlei Garcia Marques, Patrícia Cristina Ribeiro Conceição, Luís Cláudio Nascimento da Silva, Fabiana Nitz, Rosimary de Jesus Gomes Turri, Maria Rosa Quaresma Bomfim, Valério Monteiro-Neto, Hilário José Cardoso Magalhães, Antonio Dantas Silva Junior, Andrea de Souza Monteiro, Bruna Oliveira de Melo, Thiago Azevedo Feitosa Ferro, and Adrielle Zagmignan
- Subjects
0301 basic medicine ,Microbiology (medical) ,030106 microbiology ,Virulence ,Drug resistance ,Biology ,medicine.disease_cause ,Microbiology ,Article ,03 medical and health sciences ,Intergenic region ,Virology ,Multiplex polymerase chain reaction ,medicine ,lcsh:QH301-705.5 ,Pseudomonas aeruginosa ,biochemical phenomena, metabolism, and nutrition ,mcr-1 gene ,Multiple drug resistance ,oxacillinase genes ,030104 developmental biology ,extensive drug resistance (XDR) ,lcsh:Biology (General) ,Colistin ,MCR-1 ,medicine.drug - Abstract
Pseudomonas aeruginosa has caused high rates of mortality due to the appearance of strains with multidrug resistance (MDR) profiles. This study aimed to characterize the molecular profile of virulence and resistance genes in 99 isolates of P. aeruginosa recovered from different clinical specimens. The isolates were identified by the automated method Vitek2, and the antibiotic susceptibility profile was determined using different classes of antimicrobials. The genomic DNA was extracted and amplified by multiplex polymerase chain reaction (mPCR) to detect different virulence and antimicrobial resistance genes. Molecular typing was performed using the enterobacterial repetitive intergenic consensus (ERIC-PCR) technique to determine the clonal relationship among P. aeruginosa isolates. The drug susceptibility profiles of P. aeruginosa for all strains showed high levels of drug resistance, particularly, 27 (27.3%) isolates that exhibited extensively drug-resistant (XDR) profiles, and the other isolates showed MDR profiles. We detected the polymyxin E (mcr-1) gene in one strain that showed resistance against colistin. The genes that confer resistance to oxacillin (blaOXA-23 and blaOXA-51) were present in three isolates. One of these isolates carried both genes. As far as we know from the literature, this is the first report of the presence of blaOXA-23 and blaOXA-51 genes in P. aeruginosa.
- Published
- 2021