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Mammalian genomes have distinct levels of spatial organization and structure that have been hypothesized to play important roles in transcription regulation. Although much has been learned about these architectural features with ensemble techniques, single-cell studies are showing a new universal trend: Genomes are stochastic and dynamic at every level of organization. Stochastic gene expression, on the other hand, has been studied for years. In this review, we probe whether there is a causative link between the two phenomena. We specifically discuss the functionality of chromatin state, topologically associating domains (TADs), and enhancer biology in light of their stochastic nature and their specific roles in stochastic gene expression. We highlight persistent fundamental questions in this area of research.

The Pterodroma feae complex comprises three cryptically looking gadfly petrels (Cape Verde Pterodroma feae, Desertas Pterodroma deserta, and Zino’s Pterodroma madeira), once regarded as a single species, the Fea’s Petrel, P. feae. Recent research using geolocators to track and understand their movements away from breeding grounds demonstrated that both Zino’s and Desertas Petrels do undergo some long-distance movements. They perform a trans-equatorial migration to remote, previously unknown, wintering grounds, in Brazilian waters off the northeast and southern coast of Brazil during their non-breeding season. However, despite several decades of systematic and intensive beached seabird projects along extensive stretches of the Brazilian coast in these two regions, no specimens of either of these gadfly petrels have been reported. Here, we document two photographic specimen records of gadfly petrels off the Brazilian coast, provide comparative criteria for at sea identification and discuss difficulties to determine species level. First, a heavy-billed male Desertas Petrel was photographed over the continental shelf off the Rio Grande do Sul, southern Brazil, and a second specimen off Espirito Santo, southeastern Brazil, over the Vitoria Seamount, either a Desertas or a Cape Verde Petrel. Apart from Brazilian records based solely on geolocator tracking results, these seem to be the first documented specimen records for Brazil of any species within the feae-group, as well as for the southwestern Atlantic Ocean. Further, these records confirm that Brazilian offshore waters are important non-breeding feeding grounds for the feae-group.

Urochloa plantaginea and Urochloa platyphylla are common weeds in the highland area. However, in recent years, they have been found in wetlands and poorly drained soils, but the biology and behavior of the species in these conditions are not known. Thus, the objective was to assess anatomical changes in plants of Urochloa plantaginea and Urochloa platyphylla grown under different soil moisture conditions, as well as the adaptive structures generated as a result of each environment. A completely randomized experimental design in the form of a 2x2 factorial design was used, with factor A being two species of Urochloa (U. plantaginea and U. platyphylla), and species B being three soil moisture conditions (50 and 100% FC and 5 cm water depth), with four repetitions. The assessments were performed by means of anatomical cuts, observing the number and diameter (micrometers - μm) of aerenchymas in stems, roots and leaves; total diameter and the central root cylinder (μm); diameter of the fistula medulla and cortex (μm) in stems; mesophyll thickness and leaf midrib (μm). It was found that, for the two species of Urochloa, the water depth condition induced an increase in the number and diameter of aerenchymas in roots and leaves and provided a larger diameter of the fistulous pith in stems. The diameter of the central cylinder and the thickness of the leaf mesophyll midrib were more compact at 50% FC, also, for both species. Therefore, the adaptive structures generated vary as a result of the field capacity of the soil.

Sugarcane has a high yield potential; however, ensiling has been a challenge, and its fibre has low quality for ruminant feed. This study aimed to evaluate increasing levels of a fibrolytic enzymatic blend (300 U xylanase + 300 U cellulase/g) during sugarcane ensiling on fermentative profile, losses, chemical composition, in vitro degradation, and aerobic stability. Forty silos were assigned to four treatments: 0, 200, 400 and 600 mg enzymatic blend/kg sugarcane fresh matter. The trial was performed in a randomised blocked design, in which five sugarcane cultivars were defined as blocks. Silos were performed in 15-L PVC tubes and stored at room temperature for 45 days. Enzyme level did not affect silage pH, ammonia-N, soluble carbohydrates, ethanol, or organic acid concentration. Although increasing enzyme levels linearly increased effluent losses, there was no effect on gas losses or dry matter recovery. Treatment had no effect on silage chemical composition, in vitro degradation, or silage pH after aerobic exposure. However, enzyme treatment quadratically affected silage temperature after aerobic exposure and aerobic stability period. Intermediate levels of enzymes increased silage temperature after aerobic exposure and reduced the aerobic stability period. Therefore, addition of enzymes during sugarcane ensiling shows no effect on silage fermentation, nutritional value or dry matter recovery, but linearly increases effluent losses. Although an intermediate level of enzymes decreases aerobic stability, it has no effect on silage pH after aerobic exposure.

Lolium multiflorum, one of the most important temperate forage grasses in the world, is used in integrated crop-livestock systems and as a cover crop. However, it is also one of the main weeds in winter crops. The continuous use of glyphosate to manage this species has led to the selection of resistant biotypes (LOLMU-R), making it important to prevent the dispersal of these seeds. This study aimed to assess the recovery and germination of LOLMU-R that have passed through the digestive system of cattle. The experiments were carried out in metabolism cages, using a completely randomized design with six replications. The animals were given 12 112 seeds each, which were recovered from their faeces over a period of 6 days. Germination of the recovered seeds was assessed in a germination chamber and compared against a control (no animal passage). After germination, a glyphosate dose-response curve was constructed. The results obtained showed a total recovery of 1109 seeds (9.1%), with maximum recovery 2 days after ingestion, decreasing to almost zero on day 6. Germination declined linearly as a function of recovery time; however, 4 days after ingestion, germination potential was 18%. The dose-response curve proved the resistance of the recovered seeds. Cattle is a dispersal agent for LOLMU-R seeds, with animals requiring 7 days of quarantine before moving from one infested area to another.

Perger, Robert, do Amaral, Karina Bohrer, Bianchi, Filipe Michels (2021): Distribution modelling of the rare stink bug Ceratozygum horridum (Germar, 1839): isolated in small spots across the Neotropics or a continuous population? Journal of Natural History 55 (11-12): 649-663, DOI: 10.1080/00222933.2021.1919328, URL: http://dx.doi.org/10.1080/00222933.2021.1919328

O crescimento urbano desordenado causa inúmeros problemas ao ambiente em função da produção de resíduos. A problemática dos efluentes das cidades faz com que seja necessário o gerenciamento destes, sendo um dos principais desafios à destinação final. Estes resíduos podem ser utilizados na produção de substratos em viveiros de mudas. O objetivo deste trabalho foi avaliar o composto de lodo de esgoto e substrato comercial, através de diferentes misturas, em mudas de angico vermelho. O delineamento experimental foi constituído em: 100% lodo de esgoto compostado; 75% lodo de esgoto compostado e 25% substrato comercial; 50% lodo de esgoto compostado e 50% substrato comercial; 25% lodo de esgoto compostado e 75% substrato comercial e 100% substrato comercial. Foram realizadas 4 repetições de cada tratamento. Utilizaram-se parâmetros biométricos, nutricionais, de qualidade de mudas, da produção de matéria seca e dos teores finais de nutrientes contidos no substrato. A mistura de 50% lodo compostado e 50% substrato comercial apresentou os melhores valores em grande parte dos parâmetros avaliados. Estes resultados apresentam uma alternativa promissora do uso do lodo de esgoto compostado na composição de substratos para a produção de mudas de espécies florestais.

O guabijuzeiro (Myrcianthes pungens) se destaca pela abrangência em todo o território nacional, pela produção e sabor adocicado dos frutos, mas poucos estudos foram conduzidos com esta espécie até o momento. O objetivo do presente trabalho foi realizar a caracterização biométrica dos frutos e análise sensorial do suco produzido de três acessos de guabiju. A metodologia utilizada consistiu na coleta dos frutos para análises de massa de frutos, rendimento de polpa, sólidos solúveis totais e análise sensorial do suco sendo realizado teste de aceitação com 35 avaliadores não treinados. Como resultados observou-se que a média da massa de frutos foi de 3,57 g, a massa de polpa foi de 1,81 g, o rendimento de polpa foi de 49,67% e os sólidos solúveis totais foi de 14,27%. Com relação a produção de suco, o acesso 1 produziu 300 mL, o acesso 2 200 mL e o acesso 3 400 mL. Com relação a análise sensorial, o acesso 1 obteve a melhor nota de aceitação por 11,42% dos avaliadores, o acesso 2 por 80% dos avaliadores e o acesso 3 por 8,57 % dos avaliadores, o que mostra o potencial de utilização destes frutos para o desenvolvimento de sucos com frutos exóticos. Pode-se concluir que existe variação entre os acessos na biometria de frutos, produção de suco e grau de aceitação dos provadores do suco de guabiju.

Entre 23 y 27 de enero de 2020 se registró la presencia de una ballena de aleta (Balaenoptera physalus) en aguas costeras del área marina protegida del Parque Nacional Natural Corales del Rosario y de San Bernardo. Este es el primer registro de un animal vivo para el mar Caribe colombiano. Aunque no fue posible establecer relaciones de causalidad, la presente nota incluye una descripción del contexto ambiental y oceanográfico asociado a los avistamientos. Si bien el Caribe colombiano, por sus características oligotróficas, no soportaría consistentemente la presencia de los grandes rorcuales, se sugiere incluirlo como parte del rango de distribución potencial de la ballena de aleta. El presente registro también resalta la importancia y relevancia de los procesos de ciencia ciudadana y participativa, además del trabajo interinstitucional y colaborativo, acciones clave para el monitoreo de especies de alta movilidad como los mamíferos marinos.

Genome-wide studies provide increasing evidence of association of genetic variants with different behaviors. However, there is a growing need for replication and subsequent characterization of specific findings. In this sense, the CHRNA5 gene has been associated with nicotine (with genome-wide significance), alcohol and cocaine addictions. So far, this gene has not been evaluated in smoked (crack) cocaine. We aimed to analyze the influence of CHRNA5 variants in crack addiction susceptibility and severity. The sample includes 300 crack-addicted patients and 769 non-addicted individuals. The CHRNA5 SNPs evaluated were rs588765, rs16969968, and rs514743. Homozygosity for rs16969968 and rs588765 major alleles was nominally associated with a risk to crack addiction (GG, P = 0.032; CC, P = 0.036, respectively). Haplotype analyses reveal significant associations (rs588765|rs16969968|rs514743 pglobal-corrected = 7.66 × 10–5) and suggest a substantial role for rs16969968. These findings corroborate previous reports in cocaine addiction—in line with the expected effects of cocaine in the cholinergic system—and in the opposite direction of significant GWAS findings for nicotine addiction susceptibility. These results are strengthened by the first report of an association of rs588765 with crack addiction and by the haplotype findings. In summary, our study highlights the relevance of the α5 subunit on crack cocaine addiction, replicating previous results relating CHRNA5 with the genetics and pathophysiology of addiction of different drugs.

O guabijuzeiro (Myrcianthes pungens (O. Berg) d. Legrand) se destaca pela produção de frutos para consumo in natura, porém estes são pouco conhecidos. O objetivo deste trabalho foi realizar a caracterização de 22 acessos de guabijuzeiros. As plantas, folhas e frutos foram avaliados quanto a sua morfologia. Como resultados observou-se que a média da circunferência do caule foi de 69,1 cm, a altura de 5,8 m, a largura das folhas de 2,61 cm e o comprimento de 6,1 cm. Nos frutos, obteve-se as médias de circunferência de 1,65 a 1,98 cm; massa total de 2,87 g; massa de polpa de 1,28 g, de casca de 0,76 g; de semente de 2,99 g; e o grau brix foi 12,53%; e massa seca de 3,41 g. A viabilidade média do pólen foi de 95,21%. Portanto, os acessos avaliados, podem ser propagados, pois possuem ótimas características morfológicas e qualidade de frutos.

This article traces the centrality of capitalism in the work of three decolonial feminists: María Lugones, Sylvia Wynter, and Sayek Valencia. Elaborating on the role of capitalism in each of their work separately, I argue that each of these thinkers conceptualizes capitalism in a novel and urgent way, charting new directions for both theory and social movement practice. I thus argue that the decolonial feminist tradition holds crucial philosophical and historical resources for understanding the emergence of capitalism and its endurance.

The Atlantic spotted dolphin (Stenella frontalis) is endemic to tropical, subtropical, and warm temperate waters of the Atlantic Ocean. Throughout its distribution, both geographic distance and environmental variation may contribute to population structure of the species. In this study, we follow a seascape genetics approach to investigate population differentiation of Atlantic spotted dolphins based on a large worldwide dataset and the relationship with marine environmental variables. The results revealed that the Atlantic spotted dolphin exhibits population genetic structure across its distribution based on mitochondrial DNA control region (mtDNA-CR) data. Analyses based on the contemporary landscape suggested, at both the individual and population level, that the population genetic structure is consistent with the isolation-by-distance model. However, because geography and environmental matrices were correlated, and because in some, but not all analyses, we found a significant effect for the environment, we cannot rule out the addition contribution of environmental factors in structuring genetic variation. Future analyses based on nuclear data are needed to evaluate whether local processes, such as social structure and some level of philopatry within populations, may be contributing to the associations among genetic structure, geographic, and environmental distance.

Mosaic trisomy 8 is a condition characterized by a great phenotypic and cytogenetic variability whose incidence ranges around 1 in 25,000 to 50,000 live births. Here, we report a mosaic trisomy 8 patient presenting laryngotracheomalacia, an uncommon finding, analyzing its possible role over morbidity, and mortality. The patient was a boy who, after birth, had tachypnea and paleness. He presented periods of respiratory dysfunction with need of ventilatory support. Respiratory syncytial virus test was positive. Naso fibrobronchoscopy showed moderate laryngotracheomalacia. He also had recurrent episodes of pneumonia and difficulty in withdrawing continuous positive airway pressure. The patient also presented leucoma, abnormal and low-set ears, pectus excavatum, clenched fists with overlapping fingers, cryptorchidism, clubfeet, and deep longitudinal plantar creases. G-bands by Trypsin using giemsa (GTG-banding) karyotype from a peripheral blood sample revealed a mosaic trisomy 8: mos 47,XY, + 8[15]/46,XY[7]. At 4 months, the patient developed respiratory failure, and a chest computed tomography scan showed areas of atelectasis and gross fibroatelectatic striae. He ended up presenting clinical worsening and died at 4 months and 8 days. In our literature review, we found some reports describing patients with mosaic trisomy 8 and laryngotracheomalacia. However, we cannot rule out the possibility that this association could be casual, since laryngotracheomalacia is a relatively common finding in children. Therefore, more studies are still necessary to understand the possible relation between both conditions and the role of laryngotracheomalacia over morbidity and prognosis of mosaic trisomy 8 patients.

Fifty-nine Angus-cross finishing steers were used to evaluate benzoic acid, active dry yeast (Saccharomyces cerevisiae), or a combination of benzoic acid and active dry yeast when supplemented in a high-grain finishing diet on live animal performance, feeding behavior, and carcass characteristics. Steers were fed a high-grain diet for the final 106 d of finishing. Treatments were as follows: no additional supplementation (CON), 0.5% benzoic acid (ACD), 3 g per head per day active dry S. cerevisiae (YST), or both 0.5% benzoic acid and 3 g/head per day S. cerevisiae (AY). Steers were weighed every 14 d, and ultrasound was performed for rib and rump fat thickness at the beginning (day 1), middle (day 57), and end (day 99) of the experiment. Insert feeding stations were used to collect individual feeding behavior data and DMI daily throughout. Blood samples were collected on days 21 and 22 and days 99–101 to assess plane of nutrition and metabolism. Ruminal fluid samples were collected by oral gavage 4 wk prior to slaughter. Carcass characteristics were examined at a federally inspected slaughter facility. Data were analyzed using PROC GLIMMIX of SAS with initial body weight (BW) as a covariate. Benzoic acid supplementation increased (P = 0.002) overall dry matter intake (DMI) compared to YST and CON steers, which may be due to a faster eating rate (P ≤ 0.008). Animal performance parameters (BW, average daily gain, feed conversion, and ultrasound fat depth) were not different (P ≥ 0.11) among treatment groups. Aspartate aminotransferase concentration was greatest (P ≤ 0.01) for YST steers, which may have been reflected in numerically greater liver abscesses. Carcass traits did not differ (P ≥ 0.33) among treatment groups. Ruminal pH was greater (P = 0.006) for ACD steers than AY steers (pH of 6.16 vs. 5.66, respectively), which indicated that there may be an interactive effect between benzoic acid and active dry yeast. To summarize, steers fed a high-grain finishing diet supplemented with benzoic acid, active dry yeast, or both benzoic acid and active dry yeast had similar growth performance and carcass characteristics compared to those without supplementation. However, the addition of benzoic acid alone increased DMI, variation in DMI, eating rate, and ruminal pH. Future studies are warranted to further investigate the impacts of benzoic acid on the ruminal environment of feedlot cattle.

The objective of this research was to incorporate an ingredient obtained from spent cellulose casings in beef emulsion modeling systems. The test ingredient (residual sausage casing, RSC) was procured from cellulose sausage casings following thermal processing of the sausages. The casings were cleaned of contaminants before a combination of enzymatic hydrolysis and high-speed homogenization was conducted in an effort to improve the functional attributes of the cellulose casing residue (i.e. recycling/upcycling of the spent casings). The beef emulsion modeling systems used in this study consisted of 57.30% beef, 20% water, 15% olive oil, 6% of the combination of RSC and an all-purpose binder, 1.45% NaCl, 0.40% sodium tri-polyphosphate, 0.15% sodium nitrite cure, and 0.0035% sodium erythorbate. The overlying goal here was to test the ability of the RSC ingredient for partial or full replacement of binder ingredients in a beef emulsion system. Therefore, the beef emulsion model systems were prepared with five different levels of the RSC ingredient as a substitution to an all-purpose binder ingredient (0% RSC, 25% RSC, 50% RSC, 75% RSC, and 100% RSC). This study was independently replicated in its entirety three times in a completely randomized design and data were analyzed using a generalized linear mixed statistical model. Emulsion samples were tested for proximate composition, cooking loss, emulsion stability, texture profile analysis, and instrumental color. Overall, technological properties and emulsion stability were lost as the level of the RSC ingredient increased, but low inclusion levels of the RSC ingredient (25% RSC) may help maintain acceptable levels of yield and emulsion stability, while improving the sustainability of the sausage production system.

Many secondary deciduous forests of eastern North America are approaching a transition in which mature early-successional trees are declining, resulting in an uncertain future for this century-long carbon (C) sink. We initiated the Forest Accelerated Succession Experiment (FASET) at the University of Michigan Biological Station to examine the patterns and mechanisms underlying forest C cycling following the stem girdling-induced mortality of >6,700 early-successional Populus spp. (aspen) and Betula papyrifera (paper birch). Meteorological flux tower-based C cycling observations from the 33-ha treatment forest have been paired with those from a nearby unmanipulated forest since 2008. Following over a decade of observations, we revisit our core hypothesis: that net ecosystem production (NEP) would increase following the transition to mid-late-successional species dominance due to increased canopy structural complexity. Supporting our hypothesis, NEP was stable, briefly declined, and then increased relative to the control in the decade following disturbance; however, increasing NEP was not associated with rising structural complexity but rather with a rapid 1-yr recovery of total leaf area index as mid-late-successional Acer, Quercus, and Pinus assumed canopy dominance. The transition to mid-late-successional species dominance improved carbon-use efficiency (CUE = NEP/gross primary production) as ecosystem respiration declined. Similar soil respiration rates in control and treatment forests, along with species differences in leaf physiology and the rising relative growth rates of mid-late-successional species in the treatment forest, suggest changes in aboveground plant respiration and growth were primarily responsible for increases in NEP. We conclude that deciduous forests transitioning from early to middle succession are capable of sustained or increased NEP, even when experiencing extensive tree mortality. This adds to mounting evidence that aging deciduous forests in the region will function as C sinks for decades to come.

A principal fonte de alimentação dos ruminantes no Sul do Brasil é proveniente de sistemas pastoris e o azevém anual (Lolium multiflorum Lam.) tem sido utilizado como uma das principais forrageiras para pastoreio no período hibernal. O nitrogênio (N) é o principal nutriente para a manutenção da produtividade e persistência das gramíneas forrageiras, no entanto, este se encontra em baixas concentrações no solo, sendo necessário adicioná-lo na forma de fertilizante químico. Para melhor uso da fertilização, buscam-se ferramentas simples e eficientes para determinar o status nitrogenado da pastagem. O objetivo do trabalho foi determinar o índice de nutrição nitrogenada (INN) do azevém, para validar uma metodologia mais simplificada e eficiente para seu diagnóstico. Os tratamentos foram cinco doses de N (0, 25, 50, 100, 200 kg ha-1) e duas formas de aplicação do fertilizante nitrogenado (única e parcelada). As parcelas com azevém anual submetidas à adubação nitrogenada fracionada apresentaram INN abaixo de um, sugerindo limitação do nutriente no primeiro corte, exceto na dose de 200 kg ha-1 onde os resultados de INN obtidos foram próximos a um, indicando adequado fornecimento de nitrogênio às plantas. Os resultados de INN indicaram que doses de 100 kg/ha-1 de N devem ser aplicadas de forma única em pastagem de azevém anual, para a planta suprir suas necessidades de N no início do ciclo. Houve relação linear entre a concentração de N na última folha completamente expandida e o INN, indicando grande potencial no uso desse método para determinar o status nitrogenado da cultura.

The purpose of this study was to characterize the relationship between chicken breast quality and sensory attributes with chicken thigh quality and sensory attributes. Whole chicken carcasses were ...

Host resistance to Mycobacterium tuberculosis infection requires the activities of multiple leukocyte subsets, yet the roles of the different innate effector cells during tuberculosis are incompletely understood. Here we uncover an unexpected association between eosinophils and Mtb infection. In humans, eosinophils are decreased in the blood but enriched in resected human tuberculosis lung lesions and autopsy granulomas. Influx of eosinophils is also evident in infected zebrafish, mice, and nonhuman primate granulomas, where they are functionally activated and degranulate. Importantly, employing complementary genetic models of eosinophil deficiency, we demonstrate that, in mice, eosinophils are required for optimal pulmonary bacterial control and host survival after Mtb infection. Collectively, our findings uncover an unexpected recruitment of eosinophils to the infected lung tissue and a protective role for these cells in the control of Mtb infection in mice.

ContextAnthropogenic habitat disturbances that affect the ecology and behavior of parasites and hosts can either facilitate or compromise their interactions and modulate the parasite richness.ObjectivesWe assessed if the size of the habitat patch, the composition and configuration of the landscape (forest cover, patch density and mean distance to the nearest patch) and host group size influence the parasite richness of brown howler monkeys (Alouatta guariba clamitans) inhabiting forest fragments immersed in an anthropogenic matrix.MethodsWe collected fecal samples from 60 howler monkey groups inhabiting distinct forest fragments (one group/fragment) from January to July 2019. We used generalized linear models to assess the power of the independent variables in predicting parasite richness at the patch- and patch-landscape scales.ResultsWe found 10 parasite taxa (five basal eukaryotes, four nematodes and one platyhelminth), nine of which also infect humans or domestic animals. Overall parasite richness showed an inverse relationship with habitat patch size and forest cover, and a direct relationship with the mean distance to the nearest patch and group size. Patch-landscape metrics and host group size also influenced the infection with parasites with direct cycle and transmission via ingestion of the infective stage in the arboreal environment or with parasites with indirect cycle and transmission via ingestion of intermediate hosts. However, all significant models presented low weight.ConclusionsWe suggest that characteristics of parasite and host populations among other factors are more critical modulators of the relationship between howler monkeys and their parasites in anthropogenic landscapes.

Consumption of red meat contributes to the intake of many essential nutrients in the human diet including protein, essential fatty acids, and several vitamins and trace minerals, with high iron content, particularly in meats with high myoglobin content. Demand for red meat continues to increase worldwide, particularly in developing countries where food nutrient density is a concern. Dietary and genetic manipulation of livestock can influence the nutritional value of meat products, providing opportunities to enhance the nutritional value of meat. Studies have demonstrated that changes in livestock nutrition and breeding strategies can alter the nutritional value of red meat. Traditional breeding strategies, such as genetic selection, have influenced multiple carcass and meat quality attributes relevant to the nutritional value of meat including muscle and fat deposition. However, limited studies have combined both genetic and nutritional approaches. Future studies aiming to manipulate the composition of fresh meat should aim to balance potential impacts on product quality and consumer perception. Furthermore, the rapidly emerging fields of phenomics, nutrigenomics, and integrative approaches, such as livestock precision farming and systems biology, may help better understand the opportunities to improve the nutritional value of meat under both experimental and commercial conditions.

This study aimed to evaluate the effects of different pre-cutting and residual heights of Pearl millet, Sudangrass, and Sorghum pastures on yield, morphological and chemical composition. The experimental design was the randomized blocks, with four experimental units per treatment. It was evaluated three species of forage: 1) Pearl millet (MIL; Pennisetum americanum); 2) Sudangrass (SUD; Sorghum sudanense); and 3) Sorghum (SOR; Sorghum bicolor); and two cutting managements: I) 50:20 - cutting height at 50 cm and 20 cm residual height; and II) 85:10 - cutting height at 85 cm and 10 cm residual height. Managements of cutting height affected neither forage yield per cut nor total forage yield. Sudangrass had less cuts than MIL and SOR when submitted to the 50:20 management. The 85:10 management increased stem proportion and leaf to stem ratio, and reduced leaf proportion in comparison with the 50:20 management. The 50:20 management reduced forage contents of fiber and organic matter, and increased forage contents of crude protein and total digestible nutrient. Sorghum exhibited greater nutritive value due to increased leaf proportion. The 50:20 management increased number of cuts and improved nutritive value of forages, regardless of species evaluated in this study. RESUMO Este estudo foi conduzido com o objetivo de avaliar os efeitos de diferentes alturas pré e pós colheita de milheto, capim-sudão e sorgo forrageiro sobre a produção de forragens e a composição morfológica e bromatológica. O experimento foi conduzido em delineamento em blocos casualizados, com quatro unidades experimentais por tratamento. Foram avaliadas três espécies forrageiras: 1) Milheto (MIL; Pennisetum americanum); 2) Capim-Sudão (SUD; Sorghum sudanense); e 3) Sorgo (SOR; Sorghum bicolor); e dois manejos de cortes: I) 50:20: com 50 cm de altura de corte e 20 cm de altura residual; e II) 85:10: com 85 e 10 cm de altura de corte e residual, respectivamente. Foram avaliados a produção, o número de cortes, e a composição morfológica e bromatológica. Não houve diferenças entre as espécies na produção por corte e na produção total, independentemente do manejo de cortes. O SUD apresentou menor número de cortes que MIL e SOR, quando submetido ao manejo 50:20. O manejo 85:10 aumentou a proporção de caule e a razão folha:caule em relação ao manejo 50:20. O manejo 50:20 reduziu os teores de fibra e matéria orgânica, enquanto aumentou os teores de proteína bruta e nutrientes digestíveis totais na forragem. O SOR tem um maior valor nutricional devido à maior proporção de folhas. O manejo 50:20 aumenta o número de cortes e o potencial nutricional, independentemente da espécie forrageira avaliada.

The genus Dioscorea, family Dioscoreaceae, has 633 species, all popularly known as yam, of which approximately 137 contain the diosgenin compound. Diosgenin, a phytosteroid sapogenin, is used as a raw material for the synthesis of steroid hormones that make up several types of drugs, such as adrenal cortical hormone, sex hormone, birth control pills, anabolic hormones, among others. Diosgenin also presents pharmacological activities such as anti-inflammatory, antimicrobial, and hypoglycemic, which allows its utilization to treat several diseases such as osteoporosis, diabetes, and obesity. Currently, the amount of diosgenin extracted from yam species have decreased due to extensive harvesting and consequently decline of Dioscorea spp. populations, as well as the lack of adequate technologies capable of extracting this compost on a large scale. Thus, it is necessary to identify species and/or accessions of Dioscorea with a higher diosgenin content to attend the demand for diosgenin extraction. However, a uniform procedure for the preparation of samples and analysis of diosgenin is highly desirable. In this review, we are providing information about the origin, domestication, geographic distribution, cultivation, utilization, and medicinal properties of diosgenin-contained Dioscorea species.

Avaliou-se o crescimento de mudas de mirindiba (Lafoensia glyptocarpa Koehne) em diferentes concentrações de lodo de esgoto compostado (LEC), substrato comercial (SC) e lâminas de água (L). O delineamento experimental, em parcelas subdivididas, contou com 4 repetições, dispostas em 3 L e 5 diferentes tratamentos. Avaliou-se: altura, número de folhas, diâmetro do coleto, massa seca das raízes e parte aérea e o índice de qualidade de Dickson (IQD). O uso de SC + LEC apresentou os melhores resultados nos diferentes parâmetros, porém, o IQD sugere o uso de 100% LEC com irrigação L2 (25,5 mm 9 min ao dia).

In a randomized complete block design, 59 Angus-cross finishing steers were used to evaluate the effects of benzoic acid, active dry Saccharomyces cerevisiae or a combination of both when supplemented in a high-grain finishing diet on feeding behavior, animal performance, and carcass characteristics. Steers were fed for 106 d (77% high-moisture corn, 16% alfalfa haylage, 6% soybean meal, 1% mineral and vitamin supplement with salt and limestone) containing: no supplementation (CON; n = 15), 0.5% benzoic acid (ACD; n = 14), 3 g/hd/d active dry S. cerevisiae (YST; n = 15), or both 0.5% benzoic acid and 3 g/hd/d S. cerevisiae (AY; n = 11). Every 14 d steers were weighed, and ultrasound images for rib and rump fat thickness were collected at the beginning, middle, and end of the experiment. Insentec feeding stations were used to collect individual animal feeding behaviour data and DMI throughout the study. Data were analyzed using PROC GLIMMIX of SAS with initial BW as a covariate. Benzoic acid supplementation increased (P = 0.002) overall DMI compared to YST and AY steers, which was due to faster eating rates for ACD steers (P ≤ 0.008). Individual animal DMI variation was greater (P = 0.001) for ACD and AY steers. Rumen pH was greater (P = 0.006) for ACD steers vs. CON and AY steers (6.16, 5.84, and 5.66, respectively). Growth performance measures were similar (P ≥ 0.11) between treatment groups. Aspartate aminotransferase concentrations in blood were greatest (P ≤ 0.01) for YST steers, which was reflected in greater proportions of abscessed livers at slaughter. Carcass characteristics did not differ (P ≥ 0.68) between treatment groups. The results from this experiment suggest that steers fed a high grain finishing diet supplemented with benzoic acid, Saccharomyces cerevisiae or both had similar growth performance and carcass characteristics as a control group. The addition of benzoic acid increased eating rate and elevated rumen pH, which was ultimately reflected in increased DMI.

Since buffaloes are a seasonal, polyestrous species, optimizing reproduction during the non-breeding season is a key factor in increasing the reproductive and productive efficiency of herds. Ovum pick-up associated with in vitro embryo production and embryo cryopreservation is an alternative to reduce seasonal impacts. We studied the effects of seasonality in buffalo oocyte donors and embryo recipients during the favorable and non-favorable breeding seasons. Donors were evaluated for oocyte recovery and blastocyst production rate as dFBS (donors in favorable breeding season) or dNBS (donors in non-favorable breeding season). Embryos produced from dFBS or dNBS were cryopreserved by vitrification or the slow-freeze method for direct transfer and transferred to recipients in the favorable (rFBS) or non-favorable breeding season (rNBS). The heifers or cows were subjected to a fixed-time embryo transfer protocol and conception rates were determined on day 30 and on day 60. The oocyte recovery was lower in dFBS than in dNBS (7.6 vs. 10.0 oocyte/OPU, p = 0.0262); while no difference was found comparing blastocyst production rate (23.7% vs. 30.9% of blastocysts, respectively). Embryos from dFBS resulted in greater (p = 0.0013) conception rates on day 30 compared to dNBS (46.5% vs. 22.4%, respectively), despite the breeding season. The rFBS and rNBS treatments had similar (p = 0.6714) conception rates on day 30 (38.0% vs. 33.0%, respectively), indicating similar uterine receptivity. However, heifers on FBS had higher (p = 0.0003) conception rates on day 30 than cows (73.9% vs. 13.3%, respectively) when receiving embryos from dFBS. Vitrification and direct transfer had similar (p = 0.1698) conception rates on day 30 (30.4% vs. 41.4%, respectively). In conclusion, in vitro-produced embryos derived from dFBS were more competent in establishing pregnancy than dNBS counterparts, independent of recipients' reproductive seasonality. Heifers achieved better conception rates than cows during the favorable breeding season when the embryo came from dFBS. Cryopreserved in vitro produced embryos represent a reliable alternative to reduce seasonal variations in buffalo reproduction. The data elucidate the seasonal effects on embryo competence and on recipients’ uterine receptivity, affording new strategies to implement ovum pick-up associated with in vitro embryo production programs in buffalo herds.

Forage allowance impacts dry matter (DM) intake and the use of nutrients by ruminants. The efficient use of protein and energy from pasture is related to better livestock performance and lower environmental impacts. The aims of this study were to evaluate the effect of forage allowance levels on intake, digestibility, nitrogen (N) and energy balance, and methane (CH4) emissions by lambs fed fresh pearl millet [Pennisetum americanum (L.) Leeke]. An indoor trial was performed using lambs in a completely randomized design with four treatments [forage allowance at 1.5, 2.0, 2.5 kg DM/100 kg of live weight (LW), and ad libitum allowing 20% of refusals] and four replicates (lambs). Forage intake, digestibility, total urine and feces excretion, and CH4 emission were measured to calculate N and energy balances. An increase in forage allowance resulted in a linear increase in lamb forage intake, N retention, and metabolizable energy intake. Moreover, lamb CH4 emission (g/day) also increased with greater forage allowance, while CH4 yield decreased linearly as forage allowance increased. Our results indicate that maximizing forage intake improves N and energy use efficiency and mitigates CH4 yield and decreases CH4 conversion factor (Ym) by lambs fed pearl millet forage. Thus, management strategies that optimize intake of tropical forages by ruminants improve the use of nutrients ingested and mitigates negative impacts to the environment.

This study evaluated the effect of different supplements during winter on the performance of heifers in a native Pampa biome field using deferred grazing and evaluated the economic viability of each treatment. A total of 124 heifers from Brangus and Angus breeds, 18 months old, and with an average weight of 301.6 kg were included in this experiment. The treatments applied were: control with only native grassland, native grassland + 80P mineral salt treatment (MS), native grassland + protein salt treatment (PS), and native grassland + protein/energy salt treatment (PES). Besides the productive, botanical, and bromatological characteristics of the pasture, the average daily gain and the live weight gain per hectare were evaluated. The economic viability of the treatments was evaluated through partial budgeting. Weight loss was observed in all treatments. However, only the PES treatment differed from the others, with an average daily gain of -0.046 kg dia-1 (p value < 0.005). The MS treatment was the best regarding economic viability. Despite that, the smallest weight loss was observed in the PES treatment, which may be a determinant in the development and future performance of heifers. Despite the small net margin, this treatment did not incur additional costs to the system.

Immune responses to infectious agents are initiated when a pathogen or its components bind to pattern recognition receptors (PRRs). PRR binding sets off a cascade of events that activates immune responses. We now show that, in addition to activating immune responses, PRR signaling also initiates an immunosuppressive response, probably to limit inflammation. The importance of the current findings is that blockade of immunomodulatory signaling, which is mediated by the upregulation of the CD47 molecule, can lead to enhanced immune responses to any pathogen that triggers PRR signaling. Since most or all pathogens trigger PRRs, CD47 blockade could be used to speed up and strengthen both innate and adaptive immune responses when medically indicated. Such immunotherapy could be done without a requirement for knowing the HLA type of the individual, the specific antigens of the pathogen, or, in the case of bacterial infections, the antimicrobial resistance profile., It is well understood that the adaptive immune response to infectious agents includes a modulating suppressive component as well as an activating component. We now show that the very early innate response also has an immunosuppressive component. Infected cells upregulate the CD47 “don’t eat me” signal, which slows the phagocytic uptake of dying and viable cells as well as downstream antigen-presenting cell (APC) functions. A CD47 mimic that acts as an essential virulence factor is encoded by all poxviruses, but CD47 expression on infected cells was found to be upregulated even by pathogens, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), that encode no mimic. CD47 upregulation was revealed to be a host response induced by the stimulation of both endosomal and cytosolic pathogen recognition receptors (PRRs). Furthermore, proinflammatory cytokines, including those found in the plasma of hepatitis C patients, upregulated CD47 on uninfected dendritic cells, thereby linking innate modulation with downstream adaptive immune responses. Indeed, results from antibody-mediated CD47 blockade experiments as well as CD47 knockout mice revealed an immunosuppressive role for CD47 during infections with lymphocytic choriomeningitis virus and Mycobacterium tuberculosis. Since CD47 blockade operates at the level of pattern recognition receptors rather than at a pathogen or antigen-specific level, these findings identify CD47 as a novel potential immunotherapeutic target for the enhancement of immune responses to a broad range of infectious agents.

Diffusion within bacteria is often thought of as a "simple" random process by which molecules collide and interact with each other. New research however shows that this is far from the truth. Here we shed light on the complexity and importance of diffusion in bacteria, illustrating the similarities and differences of diffusive behaviors of molecules within different compartments of bacterial cells. We first describe common methodologies used to probe diffusion and the associated models and analyses. We then discuss distinct diffusive behaviors of molecules within different bacterial cellular compartments, highlighting the influence of metabolism, size, crowding, charge, binding, and more. We also explicitly discuss where further research and a united understanding of what dictates diffusive behaviors across the different compartments of the cell are required, pointing out new research avenues to pursue.

Recently, there has been an expansion of milk alternative beverages originating from plant-based sources including soy, oat, hemp, coconut, rice, and nuts, referred to as milk. This review is focused on comparing nutrient composition of milk and plant-based milk alternatives, as well as discussing considerations relevant to consumption of plant-based milk alternatives. Milk (skim, 1%, 2%, and whole bovine milk) and seventeen plant-based milk alternatives were compared for macro-nutrient composition and calcium to determine if plant-based milk alternatives could replicate the dietary role of bovine milk. Plant-based milk alternatives often present inferior nutritional substitutes of bovine milk. The protein content of the plant-based milk alternatives reviewed ranged from 5 to 100% (48% when averaged) of the protein content of whole bovine milk. Other considerations beyond protein content also warrant investigation as level of vitamins and minerals (and their absorption) tend to be less consistent with plant-based milk alternatives.

There is evidence that dopamine receptors D2 (DRD2) and D4 (DRD4) polymorphisms may influence substance use disorders (SUD) susceptibility both individually and through their influence in the formation of DRD2-DRD4 heteromers. The dopaminergic role on the vulnerability to addiction appears to be influenced by sex. A cross-sectional study with 307 crack cocaine addicts and 770 controls was conducted. The influence of DRD2 rs2283265 and DRD4 48 bp VNTR in exon 3 variants, as well as their interaction on crack cocaine addiction susceptibility and severity were evaluated in women and men separately. An association between the DRD2 T allele and crack cocaine addiction was found in women. In this same group, interaction analysis demonstrated that the presence of DRD2-T allele and concomitant absence of DRD4-7R allele were associated with risk for crack cocaine addiction. No influence of DRD2 and DRD4 variants was observed in men regarding addiction severity. This study reinforces the role of dopaminergic genes in externalizing behaviors, especially the influence of DRD2-DRD4 interaction on SUD. This is the fourth sample that independently associated the DRD2-DRD4 interaction with SUD itself or related disorders. In addition, our findings point out to a potential difference of dopaminergic neurotransmission across sex influencing addiction susceptibility.

IL-1R1 deficiency in mice causes severe susceptibility to Mycobacterium tuberculosis. Mice and macrophage cultures lacking IL-1R1 display increased bacterial growth, suggesting that phagocytes may require IL-1R1–dependent antimicrobial signals to limit intracellular M. tuberculosis replication directly. However, the myeloid-cell–intrinsic versus –extrinsic requirements for IL-1R1 to control M. tuberculosis infection in mice have not been directly addressed. Using single-cell analysis of infected cells, competitive mixed bone marrow chimeras, and IL-1R1 conditional mutant mice, we show in this article that IL-1R1 expression by pulmonary phagocytes is uncoupled from their ability to control intracellular M. tuberculosis growth. Importantly, IL-1R1–dependent control was provided to infected cells in trans by both nonhematopoietic and hematopoietic cells. Thus, IL-1R1–mediated host resistance to M. tuberculosis infection does not involve mechanisms of cell-autonomous antimicrobicidal effector functions in phagocytes but requires the cooperation between infected cells and other cells of hematopoietic or nonhematopoietic origin to promote bacterial containment and control of infection.

Multi-omic approaches are promising a broader view on cellular processes and a deeper understanding of biological systems. with strongly improved high-throughput methods the amounts of data generated have become huge, and their handling challenging. Area Covered: New bioinformatic tools and pipelines for the integration of data from different omics disciplines continue to emerge, and will support scientists to reliably interpret data in the context of biological processes. comprehensive data integration strategies will fundamentally improve systems biology and systems medicine. to present recent developments of integrative omics, the göttingen proteomics forum (gpf) organized its 6th symposium on the 23rd of november 2017, as part of a series of regular gpf symposia. more than 140 scientists attended the event that highlighted the challenges and opportunities but also the caveats of integrating data from different omics disciplines. Expert commentary: The continuous exponential growth in omics data require similar development in software solutions for handling this challenge. Integrative omics tools offer the chance to handle this challenge but profound investigations and coordinated efforts are required to boost this field.

Water stress has been identified as a key mechanism of the contemporary increase in tree mortality rates in northwestern North America. However, a detailed analysis of boreal tree hydrodynamics and their interspecific differences is still lacking. Here we examine the hydraulic behaviour of co-occurring larch (Larix laricina) and black spruce (Picea mariana), two characteristic boreal tree species, near the southern limit of the boreal ecozone in central Canada. Sap flux density (Js), concurrently recorded stem radius fluctuations and meteorological conditions are used to quantify tree hydraulic functioning and to scrutinize tree water-use strategies. Our analysis revealed asynchrony in the diel hydrodynamics of the two species with the initial rise in Js occurring 2 h earlier in larch than in black spruce. Interspecific differences in larch and black spruce crown architecture explained the observed asynchrony in their hydraulic functioning. Furthermore, the two species exhibited diverging stomatal regulation strategies with larch and black spruce employing relatively isohydric and anisohydric behaviour, respectively. Such asynchronous and diverging tree-level hydrodynamics provide new insights into the ecosystem-level complementarity in tree form and function, with implications for understanding boreal forests' water and carbon dynamics and their resilience to environmental stress.

Estuarine freshwater marshes can act as an important ecosystem for carbon storage and flux because of its strategic position in a watershed. We monitored CH4 and CO2 fluxes in Old Woman Creek, an estuarine wetland of Lake Erie, Ohio. The eddy covariance (EC) technique was used to measure fluxes of CH4 and CO2 continuously during the growing seasons of 2015 and 2016. Simultaneously, monthly sampling of gas exchange was conducted using non-steady state chambers in four distinct land-cover types in the wetland: open water, emergent vegetation (Typha spp.), floating vegetation (Nelumbo spp.) and mud flats. Chambers and EC measurements were combined to provide estimates of the continuous contributions of each land cover to the total methane emissions of the wetland. In addition, water and meteorological measurements were used to determine the most important environmental drivers of methane flux in the wetland. We found an average rate of emission from the Typha patch, the most abundant vegetated land cover, of 219.4 g CH4 C m−2 y−1, which was much higher than rates reported in similar emergent vegetation types in other wetlands. Mud flats had the highest rates of CH4 emission, followed by Nelumbo and Typha patches, and open water. Mud flats contributed 6.8% of the total CH4 emissions of the wetland despite occupying only 1.5% of the wetland area, whereas open water contributed 16.1% despite occupying 47% of the wetland area. Water temperature and wind speed were the strongest environmental drivers of CH4 flux to the atmosphere. Carbon fluxes were strongly correlated to methane fluxes. Fluctuating water levels above the wetland’s surface had a weak effect on overall CH4 emissions in the wetland, with stronger effects during the night than during the day. Providing an empirical model that predicts the influence of different environmental drivers CH4 emissions in the wetland can aid in the design of estuarine wetlands that retain nutrients and reduce coastal eutrophication while minimizing greenhouse gas emissions.

Natriuretic peptides (NPs) are known to regulate reproductive events in polyovulatory species, but their function and regulation in monovulatory species remain to be fully characterized. Using a well-established in vivo model, we found that bovine granulosa cells from follicles near the deviation stage express mRNA for the three NP receptors (NPR1, NPR2 and NPR3), but not for NP precursors (NPPA, NPPB and NPPC). The abundance of NPR3 mRNA was higher in dominant compared to subordinate follicles at the expected time of follicular deviation. After deviation, mRNA for all NP receptors was significantly more abundant in the dominant follicle. Intrafollicular inhibition of oestrogen receptors downregulated NPR1 mRNA in dominant follicles. In granulosa cells from preovulatory follicles, NPPC mRNA increased at 3 and 6 h after systemic GnRH treatment, but decreased at 12 and 24 h to similar levels observed in samples collected at 0 h. After GnRH treatment, NPR1 mRNA was upregulated at 24 h, NPR3 mRNA gradually decreased after 3 h, while NPR2 mRNA was not regulated. The mRNA expression of the enzyme FURIN increased at 24 h after GnRH treatment. These findings revealed that the expression of mRNA encoding important components of the NP system is regulated in bovine granulosa cells during follicular deviation and in response to GnRH treatment, which suggests a role of NP system in the modulation of these processes in monovulatory species.

The structural dynamics of forest canopies involve complex interactions among the abiotic environment, stand structure, species composition and disturbance regimes. How the re-arrangement of tree canopies in space and time affects forest aboveground net primary productivity (ANPP) remains poorly understood, however. In this study, we analyzed a long-term dataset from a temperate deciduous forest in Northern Michigan, USA, to investigate two primary objectives: 1) what abiotic and biotic factors influence canopy complexity and its inter-annual variability, and 2) the direct and indirect effects that abiotic, biotic and canopy complexity variables have on ANPP. We hypothesized that inter-annual variability in canopy complexity would be lower in high complexity canopies and that temporal variability in complexity metrics would be inversely related to ANPP. We found that canopy complexity was highest in more taxonomically diverse stands with high variability in tree diameters and in stands dominated by Populus tremuloides and Populus grandidentata. Canopy complexity was lowest in stands dominated by Quercus rubra and Pinus strobus, which also had lower ANPP. Stands with a high stem density had lower inter-annual variation in canopy complexity, exhibited more height growth and an increase in canopy open space, which in turn enhanced ANPP. Our results provide novel empirical evidence linking temporal stability in canopy complexity to ANPP, and suggest that variability in canopy complexity over time, in addition to the overall mean canopy complexity, may be important when considering drivers of forest carbon uptake.

The objective was to compare growth performance, belly characteristics, and bacon slicing yields of growing-finishing pigs fed a subtherapeutic dose of an antibiotic, a natural antimicrobial, or a diet containing no antibiotics or antimicrobials. Barrows and gilts (96 each, initial BW: 27.52 ± 3.98 kg) were housed in 48 pens (8 replications per treatment) in a 2 × 3 factorial randomized complete block design. Pens were assigned 1 of 3 diets: antibiotic free, oregano or tylosin phosphate. Pigs were slaughtered at an average BW of 127.31 ± 10.18 kg. There were no differences among dietary treatments for growth performance ( P ≥ 0.06), carcass cutability ( P ≥ 0.42), loin quality ( P ≥ 0.28), fresh belly dimensional characteristics ( P ≥ 0.11), IV ( P ≥ 0.87) or bacon processing characteristics ( P ≥ 0.07). Given the lack of differences in meat quality from pigs fed diets without antibiotics, the implementation of VFD in the United States should not result in changes in pork quality.

The recent outbreak of the Zika virus (ZIKV) and the discovery that perinatal Zika exposure can lead to the Congenital Zika Syndrome has promoted a call for prevention measures. Due to the increased number of babies born with microcephaly, structural brain abnormalities, and neurological alterations in regions affected by ZIKV, investigations were carried out in order to better understand this process. The maternal immune system directly influences the fetal central nervous system, and complications during pregnancy have been associated with neurodevelopmental disorders. Autism spectrum disorder (ASD), a neurodevelopmental disorder commonly manifested in the first years of life, is a disease with multifactorial etiology and is manifested typically by social and communication impairments, as well as stereotyped behaviors. Brain abnormalities, including both anatomically and functionally, can be observed in this disorder, suggesting delays in neuronal maturation and altered brain connectivity. It is known that some viral congenital infections, such as rubella, and cytomegalovirus can interfere with brain development, being associated with brain calcification, microcephaly, and ASD. Here, we reviewed a range of studies evaluating the aspects concerning brain development, immunological status during pregnancy, and neuroimmunomodulation in congenital viral infections, and we discuss if the fetal brain infection caused by ZIKV could predispose to ASD. Finally, we suggest a mechanism encompassing neurological and immunological pathways that could play a role in the development of ASD in infants after ZIKV infection in pregnancy.

The restoration projects in the Brazilian Atlantic Forest have shown more concern about genetic variability in floristic diversity in the last years. To create effective conservation policies, we need extensive knowledge on structures and patterns of genetic variation between natural populations and within them. We characterized the genetic population of Casearia sylvestris Swartz in an area under forest restoration processes (Iracemapolis, Sao Paulo) established between 1988 and 1990, and compared it to a natural remnant of semi-deciduous seasonal forest (Caetetus, Sao Paulo). The distance between locations is ∼ 210 km. One hundred twenty-eight adult plants were collected, 64 individuals in the native forest remnant and 64 in the restored fragment. The genetic data were generated by the screening of six AFLP primers combinations with a total of 296 loci for each population. The forest restoration site presented overall higher levels of genetic diversity compared to the natural remnant area. The most likely number of genetic clusters found was three (K = 3). The genetic variation was higher within populations, either in the natural or restored site, than between populations. Farther, 25 candidate outlier loci were selected and separated into two groups giving support to future research with species adaptation in these different environments. Our results show that evaluation of restored tree populations of C. sylvestris is crucial to monitoring long-term restoration programs and understand if they persist over time. We hope that our results aim at species management and the establishment of others restoration projects.

Our understanding of inherited retinal disease has benefited immensely from molecular genetic analysis over the past several decades. New technologies that allow for increasingly detailed examination of a patient’s DNA have expanded the catalog of genes and specific variants that cause retinal disease. In turn, the identification of pathogenic variants has allowed the development of gene therapies and low-cost, clinically focused genetic testing. Despite this progress, a relatively large fraction (at least 20%) of patients with clinical features suggestive of an inherited retinal disease still do not have a molecular diagnosis today. Variants that are not obviously disruptive to the codon sequence of exons can be difficult to distinguish from the background of benign human genetic variations. Some of these variants exert their pathogenic effect not by altering the primary amino acid sequence, but by modulating gene expression, isoform splicing, or other transcript-level mechanisms. While not discoverable by DNA sequencing methods alone, these variants are excellent targets for studies of the retinal transcriptome. In this review, we present an overview of the current state of pathogenic variant discovery in retinal disease and identify some of the remaining barriers. We also explore the utility of new technologies, specifically patient-derived induced pluripotent stem cell (iPSC)-based modeling, in further expanding the catalog of disease-causing variants using transcriptome-focused methods. Finally, we outline bioinformatic analysis techniques that will allow this new method of variant discovery in retinal disease. As the knowledge gleaned from previous technologies is informing targets for therapies today, we believe that integrating new technologies, such as iPSC-based modeling, into the molecular diagnosis pipeline will enable a new wave of variant discovery and expanded treatment of inherited retinal disease.

The use of oocytes recovered from prepubertal donors for in vitro embryo production has great potential for accelerating the rate of genetic gain in the dairy industry. However, these oocytes are known to be less developmentally competent than those from adult donors. In this study, we investigated the effect of age and gonadotropin stimulation in Holstein heifers subjected to oocyte collection every two weeks between 2 and 6 months of age. In order to assess the effect of gonadotropin stimulation, animals were subjected to one of three treatments, namely Short (ST; 36-42 h), Long (LT; ≥72 h) and No Treatment (NT) prior to laparoscopic ovum pick-up (LOPU). Our results show that the LT significantly improved the proportion of large follicles (5 mm diameter) present in the ovary (LT 34.0% vs. ST 11.2% vs. NT 2.4%, P 0.05), as well as the percentage of good-quality cumulus oocyte complexes (COCs) recovered (LT 95.3 ± 18% vs. ST 85.4 ± 22% vs. NT 82.2 ± 14%, P 0.05) and blastocyst rate (LT 36.7 ± 26% vs. ST 18.3 ± 15% vs. NT 16.7 ± 9%, P 0.05). Recovery rate was affected by treatment (LT 70.4 ± 25 vs. ST 85.4 ± 29 vs. NT 72.7 ± 23, P 0.05). To assess the impact of age, data was grouped into100 days (A), 100-130 days (B) and130 days (C) of age at LOPU. We found that as animals got older, although the average number of COCs per donor per LOPU declined (A: 17.5 ± 11 vs. B: 14.7 ± 7 vs. C: 11.9 ± 8), the blastocyst rate increased (A: 12.8 ± 20% vs. B: 17.1 ± 21% vs. C: 21.8 ± 25%, P 0.05). We also evaluated the incidence of polyspermy and confirmed it is a critical limitation for IVF in calf oocytes. The incidence of polyspermy was unaffected by gonadotropin treatment, but significantly decreased with age. The capacity for full development to term of in vitro produced embryos from calf oocytes was tested by embryo transfer into 21 synchronized adult recipients, which resulted in 13 pregnancies (62%), full development to term and healthy calves born. Finally, the study allowed evaluating the safety of the procedure since, on average, each animal was subjected to 8 LOPU procedures over a period of 4 months. Our results showed that the procedure is safe (no incidents during laparoscopy), and was not harmful for the reproductive future of the animals, as those that were bred became pregnant after reaching sexual maturity.