Search

Your search keyword '"Betalainic food"' showing total 2 results
Start Over Descriptor "Betalainic food" Topic biological transport
2 results on '"Betalainic food"'

1. Trans-epithelial transport of the betalain pigments indicaxanthin and betanin across Caco-2 cell monolayers and influence of food matrix.

Author

Tesoriere, L., Gentile, C., Angileri, F., Attanzio, A., Tutone, M., Allegra, M., and Livrea, M.

Subjects
*BIOLOGICAL transport, *CELL lines, *HIGH performance liquid chromatography, *INTESTINAL absorption, *T-test (Statistics), *PHYTOCHEMICALS, *QUATERNARY ammonium compounds, *DATA analysis software, *DESCRIPTIVE statistics
Abstract

Purpose: This study investigated the absorption mechanism of the phytochemicals indicaxanthin and betanin and the influence of their food matrix (cactus pear and red beet) on the intestinal transport. Methods: Trans-epithelial transport of dietary-consistent amounts of indicaxanthin and betanin in Caco-2 cell monolayers seeded on Transwell inserts was measured in apical to basolateral (AP-BL) and basolateral to apical (BL-AP) direction, under an inwardly directed pH gradient (pH 6.0/7.4, AP/BL) mimicking luminal and serosal sides of human intestinal epithelium. The effect of inhibitors of membrane transporters on the absorption was also evaluated. Contribution of the paracellular route was investigated after EDTA treatment of the cell monolayer. In vitro digestion of betalainic food was performed to provide a post-intestinal fraction containing bioaccessible pigments. Results: Apparent permeability coefficients ( P) in the absorptive direction were (4.4 ± 0.4) × 10 and (3.2 ± 0.3) × 10 cm s for indicaxanthin and betanin, respectively. Transport of indicaxanthin was non-polarized, linear as a function of time and concentration, and unaffected by inhibitors of membrane transporters. Betanin exhibited significantly different bidirectional P values and non-linear efflux kinetics. The concentration-dependent betanin efflux was described by a kinetic model including one non-saturable ( K = 0.042 μL cm min) and one saturable component identified as the apical multidrug resistance-associated protein 2 (MRP2; K = 275 μM; J = 42 pmol min cm). Permeation of both betalains increased remarkably after EDTA treatment of the cell monolayer. Neither indicaxanthin nor betanin underwent metabolic transformation. Food matrix did not affect trans-epithelial transfer of indicaxanthin, but reduced the absorption rate of betanin, red beet more than cactus pear. Conclusions: Dietary indicaxanthin and betanin can substantially be absorbed through paracellular junctions of intestinal epithelial cells. Additional trans-membrane permeation can be considered for betanin, whose absorption is limited by a MRP2-mediated efflux and negatively affected by its food matrix. Present findings are consistent with the quite higher bioavailability of indicaxanthin over betanin established in humans. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

2. Trans-epithelial transport of the betalain pigments indicaxanthin and betanin across Caco-2 cell monolayers and influence of food matrix

Author

Carla Gentile, Alessandro Attanzio, Mario Allegra, M. A. Livrea, Marco Tutone, Filippo Flavio Angileri, Luisa Tesoriere, Tesoriere, L, Gentile, C, Angileri, F, Attanzio, A, Tutone, M, Allegra, M, and Livrea, MA

Subjects
Absorption (pharmacology), Cell Membrane Permeability, Chemical Phenomena, Pyridines, Betalains, indicaxanthin, Medicine (miscellaneous), Plant Roots, Intestinal absorption, Antioxidants, Caco-2 cell, chemistry.chemical_compound, Pigment, Settore BIO/10 - Biochimica, Humans, betalains, intestinal absorption, Caco-2 cells, betalainic food, betanin, Food science, Intestinal Mucosa, Betanin, PEAR, Nutrition and Dietetics, betalain, Cell Polarity, Food Coloring Agents, Opuntia, Biological Transport, Pigments, Biological, Betaxanthins, Intercellular Junctions, chemistry, Intestinal Absorption, Caco-2, visual_art, Fruit, Food, Fortified, visual_art.visual_art_medium, ATP-Binding Cassette Transporters, Digestion, Betacyanins, Beta vulgaris, Caco-2 Cells, Indicaxanthin
Abstract

Purpose: This study investigated the absorption mechanism of the phytochemicals indicaxanthin and betanin and the influence of their food matrix (cactus pear and red beet) on the intestinal transport. Methods: Trans-epithelial transport of dietary-consistent amounts of indicaxanthin and betanin in Caco-2 cell monolayers seeded on TranswellR inserts was measured in apical to basolateral (AP-BL) and basolateral to apical (BL-AP) direction, under an inwardly directed pH gradient (pH 6.0/7.4, AP/BL) mimicking luminal and serosal sides of human intestinal epithelium. The effect of inhibitors of membrane transporters on the absorption was also evaluated. Contribution of the paracellular route was investigated after EDTA treatment of the cell monolayer. In vitro digestion of betalainic food was performed to provide a post-intestinal fraction containing bioaccessible pigments. Results: Apparent permeability coefficients (P app) in the absorptive direction were (4.4 ± 0.4) × 10-6 and (3.2 ± 0.3) × 10-6 cm s-1 for indicaxanthin and betanin, respectively. Transport of indicaxanthin was non-polarized, linear as a function of time and concentration, and unaffected by inhibitors of membrane transporters. Betanin exhibited significantly different bidirectional P app values and non-linear efflux kinetics. The concentration- dependent betanin efflux was described by a kinetic model including one non-saturable (K d = 0.042 μL cm-2 min-1) and one saturable component identified as the apical multidrug resistance-associated protein 2 (MRP2; K m = 275 μM; J max = 42 pmol min-1 cm-2). Permeation of both betalains increased remarkably after EDTA treatment of the cell monolayer. Neither indicaxanthin nor betanin underwent metabolic transformation. Food matrix did not affect trans-epithelial transfer of indicaxanthin, but reduced the absorption rate of betanin, red beet more than cactus pear. Conclusions: Dietary indicaxanthin and betanin can substantially be absorbed through paracellular junctions of intestinal epithelial cells. Additional trans-membrane permeation can be considered for betanin, whose absorption is limited by a MRP2-mediated efflux and negatively affected by its food matrix. Present findings are consistent with the quite higher bioavailability of indicaxanthin over betanin established in humans. © 2012 Springer-Verlag.

Published
2012

Catalog

Books, media, physical & digital resources
See catalog results