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Your search keyword '"Pieulle, Laetitia"' showing total 3 results
Start Over Author "Pieulle, Laetitia" Topic biological sciences
3 results on '"Pieulle, Laetitia"'

1. Multiple orientations in a physiological complex: The pyruvate-ferredoxin oxiodoreductase-ferredoxin system

Author

Pieulle, Laetitia, Nouailler, Matthieu, Morelli, Xavier, Cavazza, Christine, Gallice, Phillippe, Hatchikian, E. Claude, Guerlesquin, Francoise, Bianco, Pierre, and Blanchet, Stephane

Subjects
Pyruvates -- Research, Electron transport -- Methods, Physiological research, Biological sciences, Chemistry
Abstract

The thermodynamic properties and the electron transfer between PFOR and either native or mutated FdI are investigated. The remarkable flexibility fo the PFOR-FdI system supports the idea that the specificity of the physiological complex has probably sacrificed to improve the turnover rate and thus the efficiency.

Published
2004

2. Isolation and analysis of the gene encoding the pyruvate-ferredoxin oxidoreductase of Desulfovibrio africanus, production of the recombinant enzyme in Escherichia coli, and effect of carboxy-terminal deletions on its stability

Author

Pieulle, Laetitia, Magro, Valerie, and Hatchikian, E. Claude

Subjects
Microbial enzymes -- Research, Escherichia coli -- Research, Genetic code -- Analysis, Biological sciences
Abstract

The Pyruvate-ferredoxin oxidoreductase of the bacterium Desulfovibrio africanus, the production of the recombinant enzyme in Escherichia coli and the effect of carboxy-terminal deletions on its stability was analyzed. Results of the study revealed a stable recombinant enzyme toward oxygen for several days. These proves that the recombinant enzyme behaves like the native D. africanus enzyme.

Published
1997

3. Disulfide bond-dependent mechanism of protection against oxidative stress in pyruvate-ferredoxin oxidoreductase of anaerobic Desulfovibrio bacteria

Author

Vita, Nicolas, Hatchikian, E. Claude, Nouailler, Matthieu, Dolla, Alain, and Pieulle, Laetitia

Subjects
Oxidative stress -- Research, Pyruvate kinase -- Properties, Sulfur bacteria -- Physiological aspects, Sulfur bacteria -- Research, Biological sciences, Chemistry
Abstract

Studies on disulfide bond-dependent reversible mechanism observed in the Desulfovibrio species to protect one of the key enzymes, pyruvate-ferredoxin oxidoreductase (PFOR) against oxidative stress are reported.

Published
2008

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