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Your search keyword '"Irrgang, Klaus-Dieter"' showing total 5 results
Start Over Author "Irrgang, Klaus-Dieter" Topic biological sciences
5 results on '"Irrgang, Klaus-Dieter"'

1. Thermostability and Ca(super 2+) binding properties of wild type and heterogeneously expressed PsbO protein from cyanobacterial photosystem II

Author

Loll, Bernhard, Gerold, Gisa, Irrgang, Klaus-Dieter, Saenger, Wolfram, Slowik, Saria, Jung, Christiane, and Voelter, Wolfgang

Subjects
Circular dichroism -- Research, Calcium, Dietary -- Research, Escherichia coli -- Genetic aspects, Escherichia coli -- Research, Biological sciences, Chemistry
Abstract

The overexpression of Escherichia coli and the characterization of the gene product by biochemical and spectroscopic methods are described. recPsbO was titrated in the presence of Ca(super 2+), followed by UV-circular dichroism spectropolarimetry (CD) and Fourier transform infrared spectroscopy in order to elucidate probable Ca(super 2+) binding site(s), but no significant changes in the secondary structure could be observed.

Published
2005

2. Size determination of cyanobacterial and higher plant photosystem II by gel permeation chromatography, light scattering, and ultracentrifugation

Author

Zouni, Athina, Kern, Jan, Frank, Joachim, Hellweg, Thomas, Behlke, Joachim, Saenger, Wolfram, and Irrgang, Klaus-Dieter

Subjects
Bacteria, Thermophilic -- Research, Gel permeation chromatography -- Methods, Cyanobacteria -- Research, Biological sciences, Chemistry
Abstract

The oxygen-evolving photosystem II core complexes (PSIIcc) from the thermophilic cyanobacterium Thermosynechococcus elongatus (PSIIccTe) and the higher plant Spinacia oleracea (PSIIccSo) are isolated from the thylakoid membrane by solubilization with n-dodecyl-beta-D-maltoside, purified and characterized by gel permeation chromatography (GPC), dynamic light scattering (DLS), and analytical centrifugation (AUC). The results are discussed from a crystallization point of view.

Published
2005

3. Spectral substructure and excitonic interactions in the minor photosystem II antenna complex CP29 as revealed by nonlinear polarization spectroscopy in the freuency domain

Author

Voigt, Bernd, Irrgang, Klaus-Dieter, Ehlert, Jurgen, Beenken, Wichard, Renger, Gernot, Leupold, Dieter, and Lokstein, Heiko

Subjects
Photosynthesis research -- Analysis, Chloroplast pigments -- Physiological aspects, Absorption spectra -- Evaluation, Structure-activity relationships (Biochemistry) -- Analysis, Biological sciences, Chemistry
Abstract

Results reveal pronounced excitonic interactions between chlorophyll a and b molecules, suggesting that the spectral heterogeneity of a minor photosystem II antenna complex includes contribution of the chlorophyll molecules as well as the contribution from the pigment-protein interactions.

Published
2002

4. The relationship between the binding of dicyclohexylcarbodiimide and quenching of chlorophyl fluorescence in the light-harvesting proteins of photosystem II

Author

Ruban, Alexander V., Pesaresi, Paolo, Wacker, Ulrich, Irrgang, Klaus-Dieter J., Bassi, Roberto, and Horton, Peter

Subjects
Proteins -- Research, Chlorophyll -- Analysis, Amino acids -- Research, Biological sciences, Chemistry
Abstract

A study was conducted to examine the association between the binding of dicyclohexylcarbodiimide (DCCD) and the inhibition of chlorophyll fluorescence quenching in various light-harvesting system of photosystem II (PSII) preparations by DCCD. Results indicated that a number of amino acid residues support a critical role in regulating PSII function. Findings also showed that DCCD specifically and covalently bounded to light harvesting complexes.

Published
1998

5. Analysis of the reaction coordinate of photosynthetic water oxidation by kinetic measurements of 355 nm absorption changes at different temperatures in photosystem II preparations suspended in either H20 or D20

Author

Karge, Marc, Irrgang, Klaus-Dieter, and Renger, Gernot

Subjects
Isotopes -- Research, Oxidation-reduction reaction -- Analysis, Biological sciences, Chemistry
Abstract

The kinetic H/D isotope exchange effects on the univalent oxidation steps of the water oxidizing complex (WOC) in photosystem II membrane fragments and PSII core complexes from spinach as well as the temperature dependence of the reactions were analyzed using flash-induced absorption changes measured at different temperatures. Results have shown that the activation energies of each redox step in the WOC were dependent on the redox state and that deuteron substitutions cause only minor changes.

Published
1997

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