1. A rare biofilm dispersion strategy demonstrated by Staphylococcus aureus under oxacillin stress.
- Author
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Ahmed F, Mirani ZA, Urooj S, Noor Ul Hudda H, Janees Imdad M, Zhao Y, and Malakar PK
- Subjects
- Bacterial Proteins genetics, Bacterial Proteins metabolism, Staphylococcal Infections microbiology, Microscopy, Electron, Scanning, Humans, Staphylococcus aureus drug effects, Staphylococcus aureus genetics, Staphylococcus aureus physiology, Biofilms drug effects, Biofilms growth & development, Oxacillin pharmacology, Anti-Bacterial Agents pharmacology, Methicillin-Resistant Staphylococcus aureus drug effects, Methicillin-Resistant Staphylococcus aureus genetics, Methicillin-Resistant Staphylococcus aureus physiology, Microbial Sensitivity Tests
- Abstract
Staphylococcus aureus (S. aureus), a versatile Gram-positive bacterium, is implicated in a spectrum of infections, and its resilience is often attributed to biofilm formation. This study investigates the effect of sub-inhibitory doses of oxacillin on biofilm formation by methicillin-resistant S. aureus (MRSA). Specifically, it examines how these doses influence biofilms' development, maturation, and dispersal. The biofilm's zenith reached 48 h of incubation, followed by a noteworthy decline at 96 h and a distinctive clearance zone around biofilm-positive cells exposed to oxacillin. Scanning electron micrographs unveiled an intriguing active biofilm dispersal mechanism, a rarity in this species. Among 180 isolates, only three carrying the elusive icaD gene exhibited this phenomenon. icaD gene was absent in their counterparts. Notably, the icaD gene emerges as a distinctive marker, crucial in regulating biofilm dispersion and setting these isolates apart. The captivating interplay of oxacillin, biofilm dynamics, and genetic signatures disintegrate novel dimensions in understanding MRSA's adaptive strategies and underscores the importance of the icaD gene in engineering biofilm resilience., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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