1. Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis.
- Author
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Grattieri, Matteo, Babanova, Sofia, Santoro, Carlo, Guerrini, Edoardo, Trasatti, Stefano PM, Cristiani, Pierangela, Bestetti, Massimiliano, and Atanassov, Plamen
- Subjects
BILIRUBIN oxidase ,FUEL cells ,SEWAGE ,SILICA gel ,BIOFILMS ,ELECTRODES - Abstract
A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3 mg L
−1 of O2 in the bulk solution, decreasing to 0 mg L−1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08 mW, associated to the oxygen content. [ABSTRACT FROM AUTHOR]- Published
- 2015
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