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Start Over Author "Daniel Schad" Topic bioelectrocatalysis
2 results on '"Daniel Schad"'

1. Molecularly Imprinted Electropolymer for a Hexameric Heme Protein with Direct Electron Transfer and Peroxide Electrocatalysis

Author

Lei Peng, Aysu Yarman, Katharina J. Jetzschmann, Jae-Hun Jeoung, Daniel Schad, Holger Dobbek, Ulla Wollenberger, and Frieder W. Scheller

Subjects
molecularly imprinted polymers, self-assembled monolayer, direct electron transfer, hydrogen peroxide, bioelectrocatalysis, Chemical technology, TP1-1185
Abstract

For the first time a molecularly imprinted polymer (MIP) with direct electron transfer (DET) and bioelectrocatalytic activity of the target protein is presented. Thin films of MIPs for the recognition of a hexameric tyrosine-coordinated heme protein (HTHP) have been prepared by electropolymerization of scopoletin after oriented assembly of HTHP on a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold electrodes. Cavities which should resemble the shape and size of HTHP were formed by template removal. Rebinding of the target protein sums up the recognition by non-covalent interactions between the protein and the MIP with the electrostatic attraction of the protein by the SAM. HTHP bound to the MIP exhibits quasi-reversible DET which is reflected by a pair of well pronounced redox peaks in the cyclic voltammograms (CVs) with a formal potential of −184.4 ± 13.7 mV vs. Ag/AgCl (1 M KCl) at pH 8.0 and it was able to catalyze the cathodic reduction of peroxide. At saturation the MIP films show a 12-fold higher electroactive surface concentration of HTHP than the non-imprinted polymer (NIP).

Published
2016
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2. Molecularly Imprinted Electropolymer for a Hexameric Heme Protein with Direct Electron Transfer and Peroxide Electrocatalysis

Author

Katharina J. Jetzschmann, Frieder W. Scheller, Ulla Wollenberger, Jae-Hun Jeoung, Lei Peng, Aysu Yarman, Holger Dobbek, Daniel Schad, and Publica

Subjects
Hemeproteins, bioelectrocatalysis, Hemeprotein, Polymers, Surface Properties, hydrogen peroxide, Biosensing Techniques, 02 engineering and technology, lcsh:Chemical technology, 010402 general chemistry, Photochemistry, 01 natural sciences, Biochemistry, Peroxide, Redox, Article, Catalysis, Analytical Chemistry, Electron Transport, Molecular Imprinting, chemistry.chemical_compound, Electron transfer, Monolayer, Organic chemistry, lcsh:TP1-1185, direct electron transfer, Electrical and Electronic Engineering, Electrodes, Instrumentation, Institut für Biochemie und Biologie, bioelectroanalysis, Chemistry, Molecularly imprinted polymer, Self-assembled monolayer, 021001 nanoscience & nanotechnology, Atomic and Molecular Physics, and Optics, Peroxides, 0104 chemical sciences, self-assembled monolayer, molecularly imprinted polymers, Gold, ddc:620, 0210 nano-technology, Molecular imprinting
Abstract

For the first time a molecularly imprinted polymer (MIP) with direct electron transfer (DET) and bioelectrocatalytic activity of the target protein is presented. Thin films of MIPs for the recognition of a hexameric tyrosine-coordinated heme protein (HTHP) have been prepared by electropolymerization of scopoletin after oriented assembly of HTHP on a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold electrodes. Cavities which should resemble the shape and size of HTHP were formed by template removal. Rebinding of the target protein sums up the recognition by non-covalent interactions between the protein and the MIP with the electrostatic attraction of the protein by the SAM. HTHP bound to the MIP exhibits quasi-reversible DET which is reflected by a pair of well pronounced redox peaks in the cyclic voltammograms (CVs) with a formal potential of −184.4 ± 13.7 mV vs. Ag/AgCl (1 M KCl) at pH 8.0 and it was able to catalyze the cathodic reduction of peroxide. At saturation the MIP films show a 12-fold higher electroactive surface concentration of HTHP than the non-imprinted polymer (NIP).

Published
2016

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