9 results on '"Wakeman, Kevin C."'
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2. Biodiversity of symbiotic microalgae associated with meiofaunal marine acoels in Southern Japan.
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Siratee Riewluang and Wakeman, Kevin C.
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MICROALGAE ,BIODIVERSITY ,DINOFLAGELLATES ,SYMBIODINIUM ,SPECIES ,DUNALIELLA - Abstract
Acoels in the family Convolutidae are commonly found with microalgal symbionts. Convolutids can host green algal Tetraselmis and dinoflagellates within the family Symbiodiniaceae and the genus Amphidinium. The diversity of these microalgae has not been well surveyed. In this study, we used PCR and culture techniques to demonstrate the biodiversity of Tetraselmis and dinoflagellates in symbiosis with meiofaunal acoels. Here, 66 acoels were collected from seven localities around Okinawa, Ishigaki, and Kochi, Japan. While convolutids were heavily represented in this sampling, some acoels formed a clade outside Convolutidae and are potentially a new family of acoels harboring symbiotic microalgae. From the acoels collected, a total of 32 Tetraselmis and 26 Symbiodiniaceae cultures were established. Molecular phylogenies were constructed from cultured material (and from total host DNA) using the 18S rRNA gene (Tetraselmis) and 28S rRNA gene (dinoflagellates). The majority of Tetraselmis sequences grouped within the T. astigmatica clade but strains closely related to T. convolutae, T. marina, and T. gracilis were also observed. This is the first report of Tetraselmis species, other than T. convolutae, naturally associating with acoels. For dinoflagellates, members of Cladocopium and Miliolidium were observed, but most Symbiodiniaceae sequences formed clusters within Symbiodinium, grouping with S. natans, or sister to S. tridacnidorum. Several new Symbiodinium sequences from this study may represent novel species. This is the first molecular record of Miliolidium and Symbiodinium from acoels. Microalgal strains from this study will provide a necessary framework for future taxonomic studies and research on symbiotic relationships between acoels and microalgae. [ABSTRACT FROM AUTHOR]
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- 2023
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3. Phylogeny of Amphidinium (Dinophyceae) from Guam and Okinawa, with descriptions of A. pagoense sp. nov. and A. uduigamense sp. nov.
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Phua, Yong Heng, Husnik, Filip, Lemer, Sarah, and Wakeman, Kevin C.
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PHYLOGENY ,DINOFLAGELLATES ,CELL morphology ,CELL size ,ELECTRON microscopy ,MARINE toxins - Abstract
Marine benthic dinoflagellates within the genus Amphidinium were isolated from Guam and Okinawa. Isolated strains were identified to species-level using phylogenetic analyses of 28S rRNA and ITS-5.8S rRNA genes as well as microscopy. Of the six isolated strains, two were new species: A. pagoense sp. nov. and A. uduigamense sp. nov. Other isolates included strains of A. massartii and A. operculatum from Guam, and two strains of A. operculatum from Okinawa. Both new species were described using light and electron microscopy (SEM and TEM). The combination of characteristics that make A. pagoense sp. nov. unique includes a pair of centrally-located pyrenoids, variable cell shape, absence of scales and a long, curved ventral ridge. For A. uduigamense sp. nov., a combination of several morphological features distinguishes it from other species. These include a constriction near the anterior of the hypocone, two centrally located pyrenoids, a longitudinal flagellum inserted in the posterior one-third of the cell, cell size, cell division in the motile stage and the absence of scales. Toxicity was confirmed in these two novel species by testing methanol extracts in an Artemia bioassay. Previously unrecorded ITS rRNA gene sequences from A. operculatum were also sequenced from both locations. Species identified and newly described in this study expand the taxonomic knowledge of Amphidinium in the Pacific. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Rissoella golikovi
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Chira Siadén, Luis E., Wakeman, Kevin C., Webb, Stephen C., Hasegawa, Kazunori, and Kajihara, Hiroshi
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Rissoellaceae ,Rissoella golikovi ,Florideophyceae ,Gigartinales ,Rhodophyta ,Biodiversity ,Rissoella ,Plantae ,Taxonomy - Abstract
Rissoella golikovi (Gulbin, 1979) (Figs 3 E–F, 5A–G) Jeffreysina golikovi Gulbin, 1979: 89, figs. 1–2 (holotype ZIN 41388 /1; Vanino Bay, Sea of Japan, Russia); Kantor & Sysoev, 2006: 248, pl. 123, fig. E (paratype); Hasegawa, 2017: 398, 1063, pl. 355, fig. 7. Type material not available for analyses. Material examined. Fourteen mature specimens (ICHUM RT3001, RT 3002, RT3003, RO 3001, RO 3002, RO 3003, RK3001, RM 3001, RM 3002, RM 3003, ROM 3001, RA3001, RA 3002, RA 3003). For information on specimens collection locality and GenBank accession numbers see Table 1. Description. Shell minute, smaller (296–450 µm) in comparison to other rissoellids, thin, extremely fragile, translucent or whitish opaque, skeneiform (width about 150% of length), with deep, widely perforate umbilicus (Fig. 5A). Protoconch smooth, of about one whorl (Figs. 5B, C). Teleoconch smooth with distinct growth lines, slightly deep suture, of about three convex whorls; aperture simple, entire, nearly circular but with margin adjacent to previous whorl flattened. Operculum typical of family (Fig. 5D). Head–foot brown or dark grey with colorless sole; oral lobes short; cephalic tentacles slightly longer than oral lobes; oral lobes and cephalic tentacles proximally having similar coloration to head, gradually becoming transparent in distal portion. Mantle dark brown or black pigmented, with black or darker brown patch on center of dorsal portion of body whorl; another smaller dark patch placed on left of neck (Figs. 3E, F). Radular formula 12–13 × 1.R.1 (Fig. 5E). Central tooth higher than wide (width about 52% of length), with medial narrow ridge, cutting edge with one small central sharp cusp flanked by several larger cusps (Figs. 5F, G). Lateral teeth triangular (width about 41% of length), each with median ridge; cutting edge with larger median cusp, flanked by 4–5 sharp cusps, consecutively decreasing in size (Figs. 5E, F). Distribution and microhabitat. Known from Vanino Bay, as well as middle Kurile Islands, Russia. Material in this study was collected from Hokkaido, Japan: Otaru and Kamoenai (Sea of Japan), near Omu (Sea of Okhotsk), and Akkeshi and Muroran (Pacific). It was found on various algae including the coralline algae Corallina spp. Remarks. The type material of Rissoella golikovi was not examined, due to restrictions on shipping biological material. However, our newly sampled material agrees with its description. The present morphospecies is nearly identical in radula morphology to Rissoella globularis (Forbes & Hanley), which has been reported from France to northern Norway, as illustrated by Sars (1878). Nevertheless, these two species can clearly be distinguished by shell morphology. In R. globularis, the shell is depressed conical, while it is skeneiform in R. golikovi. The skeneiform shell of this species makes it easily distinguishable from other species in the family where shells are either ovate or elongate. Rissoella golikovi was first described by Gulbin (1979) from Vanino Bay, Russia, and was subsequently recorded from the eastern part of Hokkaido (Hasegawa 2017)., Published as part of Chira Siadén, Luis E., Wakeman, Kevin C., Webb, Stephen C., Hasegawa, Kazunori & Kajihara, Hiroshi, 2019, Morphological and molecular diversity of rissoellids (Mollusca, Gastropoda, Heterobranchia) from the Northwest Pacific island of Hokkaido, Japan, pp. 415-431 in Zootaxa 4551 (4) on pages 419-420, DOI: 10.11646/zootaxa.4551.4.2, http://zenodo.org/record/2623029, {"references":["Gulbin, V. V. (1979) A new species of the littoral gastropod Rissoellidae from the Far-eastern seas. Academy of Sciences of the USSR, Vladivostok. Far East Science Center. Marine Biology, 3, 88 - 89. [in Russian]","Kantor, Y. I. & Sysoev, A. V. (2006) Marine and Brackish Water Gastropoda of Russia and Adjacent Countries: An Illustrated Catalogue. KMK Scientific Pres Ltd., Moscow, 371 pp., 140 pls.","Hasegawa, K. (2017) Rissoellidae. In: Okutani, T. (Ed.), Marine Mollusks in Japan. 2 nd Edition. Tokai University Press, Tokyo, pp. 398 + 1063.","Sars, G. O. (1878) Mollusca regionis Arcticae Norvegiae. In: Bidrag til Kundskaben om Norges Arktiske Fauna. Chritiania, Brogger, 466 pp."]}
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- 2019
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5. Rissoella elatior
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Chira Siadén, Luis E., Wakeman, Kevin C., Webb, Stephen C., Hasegawa, Kazunori, and Kajihara, Hiroshi
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Rissoella elatior ,Rissoellaceae ,Florideophyceae ,Gigartinales ,Rhodophyta ,Biodiversity ,Rissoella ,Plantae ,Taxonomy - Abstract
Rissoella elatior (Golikov, Gulbin & Sirenko, 1987) (Figs 2 A–D, 3A–H) Jeffreysina elatior Golikov, Gulbin & Sirenko, 1987: 35, pl. 3, fig. 6; Kantor & Sysoev, 2006: 248, pl. 123, fig. D (holotype 36525/1; Moneron Island, Russia). Type material not available for analyses. Jeffreysiella elatior — Hasegawa, 2017: 398, 1063, pl. 355, fig. 6. Material examined. Thirteen mature specimens (ICHUM RT1001, RT 1002, RO 1001, RO 1002, RO 1003, RSH 1001, RSH 1002, RK1001, RK1002, RM 1001, RSU 1001, RSE 1001, and RSE 1002). For information on specimens collection locality and GenBank accession numbers see Table 1. Description. Shell minute (800–1270 µm) but relatively larger if compared to other species described here, thin, fragile, translucent or whitish opaque, elongate (width about 63% of length), with narrow umbilicus, spire of about 25% of total length (Fig. 4A). Protoconch smooth, slightly pointed, of approximately one whorl, without sculpture at suture (Figs. 4B, C). Teleoconch smooth except for faint markings of growth lines; with deep suture; up to 3 ½ convex whorls; aperture simple, entire, semicircular, slightly shorter or almost 50% of total length. Operculum typical of family (Fig. 4D). Head–foot opaque white, with slender oral lobes and longer cephalic tentacles. Mantle brown or black pigmented, with black patch centrally placed on dorsal portion of body whorl. Black patch hardly recognized in specimens with black mantle (Figs. 3 B–D). Radular formula 15–16 × 2.1.R.1.2 (Fig. 4E). Central tooth wide (width about 61% of length), with 7–8 sharp cusps, latter gradually increasing in size from left to right until 6th (or in some cases 7th); right-most cusp slightly smaller than left ones. Group of 10–13 minute secondary cusps encircling upper margin of last right cusp (Figs. 4E, F). Lateral teeth elongate-triangular (width about 78% of length), each with large, sharp, smooth median cusp, and 8–12 smaller cusps along inner and outer margins (Figs. 4E, G). Inner marginal teeth represented by small, curved plates (width about 93% of length), each with large, sharp, smooth median cusp, flanked by 4–5 (along inner margin) or 5–7 (along outer margin) smaller cusps (Figs. 4E, H). Outer marginal teeth reduced, simple, plate-like (width almost 200% of length) (Figs. 4E, H). Distribution and microhabitat. Originally reported from the northern part of the Sea of Japan (Moneron Island) (Golikov et al. 1987), Russia, and subsequently reported to be widely distributed along the Japanese Archipelago from Hokkaido to Miyako Island, Okinawa (Hasegawa 2017). Material in this study was collected from Rumoi to Setana on the Sea of Japan; and on the Pacific coast near Muroran, Japan. It was found on various algae including the coralline algae Corallina spp. Remarks. Although the type specimens of Rissoella elatior have not been examined in this work, our newly sampled material agrees with the original description of this species (Golikov et al. 1987), as well as the photograph of the holotype (Kantor & Sysoev 2006: pl. 123, fig. D). In some specimens (Fig. 3A) the mantle coloration is brighter than others (Figs. 3 B–D), the former being pale brown with yellowish white asymmetrical patches and a brown patch centrally placed on the dorsal portion of the body whorl., Published as part of Chira Siadén, Luis E., Wakeman, Kevin C., Webb, Stephen C., Hasegawa, Kazunori & Kajihara, Hiroshi, 2019, Morphological and molecular diversity of rissoellids (Mollusca, Gastropoda, Heterobranchia) from the Northwest Pacific island of Hokkaido, Japan, pp. 415-431 in Zootaxa 4551 (4) on pages 418-419, DOI: 10.11646/zootaxa.4551.4.2, http://zenodo.org/record/2623029, {"references":["Golikov, A. N., Gulbin, V. V. & Sirenko, B. I. (1987) Prosobranch from Moneron Island shelf (Sea of Japan). I. Orders Patelliformes-Calyptraeiformes. In: Fauna and Distribution of Molluscs, North Pacific and Polar Basin, edited by A. Kafanov. Far Eastern Science Center of the USSR Academy of Science, Moscow, pp. 20 - 40.","Kantor, Y. I. & Sysoev, A. V. (2006) Marine and Brackish Water Gastropoda of Russia and Adjacent Countries: An Illustrated Catalogue. KMK Scientific Pres Ltd., Moscow, 371 pp., 140 pls.","Hasegawa, K. (2017) Rissoellidae. In: Okutani, T. (Ed.), Marine Mollusks in Japan. 2 nd Edition. Tokai University Press, Tokyo, pp. 398 + 1063."]}
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- 2019
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6. Rissoella Chira Siadén & Wakeman & Webb & Hasegawa & Kajihara 2019
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Chira Siadén, Luis E., Wakeman, Kevin C., Webb, Stephen C., Hasegawa, Kazunori, and Kajihara, Hiroshi
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Rissoellaceae ,Florideophyceae ,Gigartinales ,Rhodophyta ,Biodiversity ,Rissoella ,Plantae ,Taxonomy - Abstract
Rissoella sp. 1 (Figs 3J, 3K) Material examined. Two mature specimens: ICHUM RSH 4001, RSH 4002; Shakotan, Hokkaido, Japan, 43°18′06.2″N 140°35′55.6″E, 25 August 2017. Both specimens were used for DNA extraction. For further information on specimens collection locality and GenBank accession numbers see Table 1. Remarks. Shell minute, thin, fragile, translucent or whitish, elongate. Head–foot translucent white; with very short round oral lobes, long cephalic tentacles with tapering tip, translucent as well. Mantle pigmented in light brown with three or four big yellowish asymmetrical patches. Big black patch, with few small whitish blotches inside, centrally placed on the dorsal portion of body whorl. Visceral mass dark brown to black, with several elongate whitish blotches (Figs. 3J, K). Distribution and microhabitat. Found in Shakotan, Hokkaido, Japan; in the subtidal zone on red algae Gelidium spp.
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- 2019
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7. Rissoella Chira Siad��n & Wakeman & Webb & Hasegawa & Kajihara 2019
- Author
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Chira Siad��n, Luis E., Wakeman, Kevin C., Webb, Stephen C., Hasegawa, Kazunori, and Kajihara, Hiroshi
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Rissoellaceae ,Florideophyceae ,Gigartinales ,Rhodophyta ,Biodiversity ,Rissoella ,Plantae ,Taxonomy - Abstract
Rissoella sp. 1 (Figs 3J, 3K) Material examined. Two mature specimens: ICHUM RSH 4001, RSH 4002; Shakotan, Hokkaido, Japan, 43��18���06.2���N 140��35���55.6���E, 25 August 2017. Both specimens were used for DNA extraction. For further information on specimens collection locality and GenBank accession numbers see Table 1. Remarks. Shell minute, thin, fragile, translucent or whitish, elongate. Head���foot translucent white; with very short round oral lobes, long cephalic tentacles with tapering tip, translucent as well. Mantle pigmented in light brown with three or four big yellowish asymmetrical patches. Big black patch, with few small whitish blotches inside, centrally placed on the dorsal portion of body whorl. Visceral mass dark brown to black, with several elongate whitish blotches (Figs. 3J, K). Distribution and microhabitat. Found in Shakotan, Hokkaido, Japan; in the subtidal zone on red algae Gelidium spp., Published as part of Chira Siad��n, Luis E., Wakeman, Kevin C., Webb, Stephen C., Hasegawa, Kazunori & Kajihara, Hiroshi, 2019, Morphological and molecular diversity of rissoellids (Mollusca, Gastropoda, Heterobranchia) from the Northwest Pacific island of Hokkaido, Japan, pp. 415-431 in Zootaxa 4551 (4) on page 423, DOI: 10.11646/zootaxa.4551.4.2, http://zenodo.org/record/2623029
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- 2019
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8. Rissoella japonica Chira Siad��n & Wakeman & Webb & Hasegawa & Kajihara 2019, n. sp
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Chira Siad��n, Luis E., Wakeman, Kevin C., Webb, Stephen C., Hasegawa, Kazunori, and Kajihara, Hiroshi
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Rissoellaceae ,Florideophyceae ,Gigartinales ,Rhodophyta ,Biodiversity ,Rissoella ,Plantae ,Taxonomy ,Rissoella japonica - Abstract
Rissoella japonica Chira & Hasegawa, n. sp. (Figs 3 G���I, 6A���G) Rissoella sp.��� Hasegawa, 2000: 700 -701, plate 349, fig. Rissoellidae-1; Hasegawa, 2017: 398, 1063, pl. 355, fig. 5. Type material. Holotype: adult, 0.9 mm (ICHUM RK2001); Kamoenai, Hokkaido, Japan, 43��08���10.5���N 140��25���43.1���E, 6 November 2016. Paratypes: 3 specimens (ICHUM RK2002, RO2001, RO2002) from Oshoro Bay, Hokkaido, Japan; 1 specimen (RSH2001); Shakotan, Hokkaido, Japan. For information on specimens collection locality and GenBank accession numbers see Table 1. ZooBank registration: urn:lsid:zoobank.org:act: E3102674-B307-40F6-A700-7696AE32FCA8 Etymology. The species name, Rissoella japonica, refers to the geographical distribution from where the species was found. Diagnosis. Protoconch with rippled sculpture at suture. Radula, central tooth with 10-13 sharp cusps on cutting edge. Lateral teeth narrow, with median ridge becoming basal process, and outer lateral projection on base; cutting edge with major median cusp, flanked by 5-6 (along outer margin) or 7-9 (along inner margin) sharp cusps. Marginal teeth similar in shape to lateral one but smaller, cutting edge with median cusp, flanked by 3-5 smaller sharp cusps on each side. Description. Shell minute (764���1091 ��m), thin, fragile, translucent or whitish opaque, elongate (width about 67% of length), with narrow umbilicus, spire of about 30% of total length (Fig. 6A). Protoconch smooth, of about 1 whorl, with rippled sculpture along suture (Figs. 6B, C). Teleoconch smooth, with distinct growth lines, deep suture, about 2 �� convex whorls; aperture simple, entire, semicircular, slightly longer than 50% of total length. Operculum typical of family (Fig. 6D). Head���foot dark brown with colorless sole; oral lobes and tentacles dark brown. Mantle dark brown or black pigmented, with black patch placed slightly to left on dorsal portion of body whorl (Figs. 3 G-I). Radular formula 11-13 �� 1.1.R.1.1 (Fig. 6E). Central tooth higher than wide (width about 48% of length), cutting edge with 10���13 sharp cusps of different sizes (Figs. 6E, F). Lateral teeth narrow (width about 23% of length), with median ridge becoming basal process, outer lateral projection on base; cutting edge with larger median cusp, flanked by 5���6 (along outer margin) or 7���9 (along inner margin) sharp cusps (Figs. 6E, G). Marginal teeth with similar shape to lateral one but smaller (width about 33% of length); cutting edge with median cusp, flanked by 3���5 smaller sharp cusps on each side (Figs. 6E, G). Distribution and microhabitat. In the Sea of Japan from Otaru to Setana, Japan. It was found in the intertidal zone on various algae including the coralline algae Corallina spp. Remarks. Both R. japonica n. sp. and R. elatior occur sympatrically in some localities, and they might be confused. However, they can be distinguished by the head-foot coloration (being dark brown in R. japonica n. sp. and white in R. elatior) and the radula morphology, as well as by conchological characters such as spire/total length and aperture/total length ratios. Based on radula morphology, R. japonica n. sp. belongs to a group containing the type species of Rissoella s.s., R. diaphana illustrated by Thiele (1929 ���1935; as R. glabra), in having a symmetrical configuration with five teeth per row. Rissoella japonica n. sp. can be distinguished from R. diaphana by the relatively narrower and smaller central tooth., Published as part of Chira Siad��n, Luis E., Wakeman, Kevin C., Webb, Stephen C., Hasegawa, Kazunori & Kajihara, Hiroshi, 2019, Morphological and molecular diversity of rissoellids (Mollusca, Gastropoda, Heterobranchia) from the Northwest Pacific island of Hokkaido, Japan, pp. 415-431 in Zootaxa 4551 (4) on page 423, DOI: 10.11646/zootaxa.4551.4.2, http://zenodo.org/record/2623029, {"references":["Hasegawa, K. (2000) Rissoellidae. In: Okutani, T. (Ed.), Marine Mollusks in Japan. Tokai University Press, Tokyo, pp. 700 - 701.","Hasegawa, K. (2017) Rissoellidae. In: Okutani, T. (Ed.), Marine Mollusks in Japan. 2 nd Edition. Tokai University Press, Tokyo, pp. 398 + 1063.","Thiele, J. (1929 - 1935) Handbuch der systematischen Weichtierkunde. Gustav Fischer, Jena, 1154 pp."]}
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- 2019
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9. Morphology and molecular phylogeny of the benthic dinoflagellates (Dinophyceae, Peridiniales) Amphidiniopsis crumena n. sp. and Amphidiniopsis nileribanjensis n. sp.
- Author
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Yamaguchi, Aika, Hoppenrath, Mona, Murray, Shauna, Kretzschmar, Anna Liza, Horiguchi, Takeo, and Wakeman, Kevin C.
- Subjects
DINOFLAGELLATES ,MOLECULAR phylogeny ,MORPHOLOGY ,RECOMBINANT DNA - Abstract
Amphidiniopsis is a benthic, heterotrophic and thecate dinoflagellate genus that has a smaller epitheca and larger hypotheca. The genus contains 24 described species, but is considered to be polyphyletic based on morphological characters and molecular phylogenetics. In this study, two new species were discovered from two distant sampling localities, Amphidiniopsis crumena sp. nov. from Japan, and Amphidiniopsis nileribanjensis sp. nov., from Australia. These species have a uniquely shaped, additional second postcingular plate. Both species are dorsoventrally flattened, an apical hook is present, and have six postcingular plates. The plate formula is: APC 4′ 3a 7″ ?C 4?S 6″′ 2″″. The cells of these species were examined with LM and SEM, and molecular phylogenic analyses were performed using 18S and 28S rDNA. These species are distinguished by the presence of spines on the hypotheca and touching of the sixth postcingular plate and the anterior sulcal plate. Their shape and disposition of several thecal plates also differ. Molecular phylogenetic analyses showed that the two new species formed a monophyletic clade and did not belong to any morphogroup proposed by previous studies. Considering the morphological features and the molecular phylogenetic results, a new morphogroup is proposed, Amphidiniopsis morphogroup VI (' crumena group'). [ABSTRACT FROM AUTHOR]
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- 2023
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