1. Biocompatible surgical meshes based on decellularized human amniotic membrane.
- Author
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Shi P, Gao M, Shen Q, Hou L, Zhu Y, and Wang J
- Subjects
- Animals, Deoxyribonucleases metabolism, Extracellular Matrix, Humans, Lipase metabolism, Materials Testing, Myocytes, Smooth Muscle cytology, Myocytes, Smooth Muscle metabolism, Polyesters chemistry, Polypropylenes chemistry, Polyurethanes chemistry, Prostheses and Implants, Rabbits, Amnion chemistry, Biocompatible Materials chemistry, Surgical Mesh
- Abstract
Meshes play important roles to repair human tissue defect. In this work, human amniotic membrane (HAM) was decellularized and explored the efficacy as an implantable biological mesh. Surfactant, hypertonic saline, lipase and DNAase were used individually or collectively to remove all cell components and remain the extracellular matrix. Results of H&E and DAPI staining demonstrated that the method of surfactant and lipase combining with DNAase is the most effective treatment for HAM decellularization. Primary smooth muscle cells were seeded to evaluate the decellularized HAM's (dHAM) in vitro cytocompatibility. The in vivo test was performed via implantation at rabbits' uterus with clinic polypropylene mesh (PP) as the control. The results indicated that dHAM possessed good biocompatibility and will be a potential candidate for biological mesh., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
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