1. Acute Toxicological and Biodistribution Aspects of Superparamagnetic Magnetite Nanoparticles In Vitro and on Animal Tissues
- Author
-
Fabiana A. Carneiro, Raquel Moraes Soares, Leandra Santos Baptista, Maria de Fátima Santos de Souza, and Luiz A. S. de Oliveira
- Subjects
Biodistribution ,Biocompatibility ,Chemistry ,In vivo ,Toxicity ,Biomedical Engineering ,Biophysics ,Nanoparticle ,Bioengineering ,MTT assay ,In vitro ,Superparamagnetism - Abstract
Superparamagnetic iron oxide nanoparticles (SPIONs) have been considered potential candidates for various therapeutic applications. For safe clinical use, it requires in vitro and in vivo studies to determine its biodistribution, toxicological profile, and biocompatibility. Another important factor is the removal of iron throughout the degradation of the nanoparticles since the excess of iron induces toxicity. This study characterized magnetite-based SPIONs coated with stabilizing polymer PVA by XRD, TEM, FTIR, and magnetometry. Posteriorly, the SPIONs were injected intraperitoneally into Balb-C mice to perform two acute assays with the following goals: (1) magnetic detection of SPIONs in tissues after exposure to different doses, (2) histopathology and detection in tissues after exposure to a single dose of SPIONs, and (3) MTT assay with HepG2 cells. Nanoparticles quantification by the Superconducting Quantum Interference Device (SQUID) showed a significant increase (p
- Published
- 2021
- Full Text
- View/download PDF