Search

At present there is a growing need for tissue engineering products, including the products of scaffold-technologies. Biopolymer hydrogel scaffolds have a number of advantages and are increasingly being used to provide means of cell transfer for therapeutic treatments and for inducing tissue regeneration. This work presents original hydrogel biopolymer scaffolds based on a blood plasma cryoprecipitate and collagen and formed under conditions of enzymatic hydrolysis. Two differently originated collagens were used for the scaffold formation. During this work the structural and mechanical characteristics of the scaffold were studied. It was found that, depending on the origin of collagen, scaffolds possess differences in their structural and mechanical characteristics. Both types of hydrogel scaffolds have good biocompatibility and provide conditions that maintain the three-dimensional growth of adipose tissue stem cells. Hence, scaffolds based on such a blood plasma cryoprecipitate and collagen have good prospects as cell carriers and can be widely used in regenerative medicine., Graphical abstract Image 1, Highlights • Original hydrogel scaffolds based on a blood plasma cryoprecipitate and collagen. • Depending on the origin of collagen, scaffolds possess differences in their properties. • Scaffolds can provide the conditions needed to maintain growth of stem cells.

To make ceramic antibacterial biocompatible material, we synthesized mixed calcium–bismuth oxophosphate (CaBiPO-apatite) with apatite structure. A mixture of CaBiPO-apatite and ammonium carbonate was used to prepare ceramic material in tablet form. Qualitative and quantitative phase analyses using powder XRD combined with IR spectroscopy shown the absence of any phase or chemical changes in the material during the process of making ceramics. In vitro experiment with human mesenchymal stromal cells showed high biocompatibility of the material caused by structural type and high porosity of material, despite the presence of bismuth in composition of the substance.

Hydroxyapatite nanoparticles were synthesized using modified sol–gel approach. Its biocompatibility was proved by in vitro experiments with diploid fibroblasts. The experiment showed that the presence of hydroxyapatite in the environment of cells does not reduce their viability. The observed phase transition of hydroxyapatite was explained as order/disorder transition, saving the crystal structure symmetry. Organo–inorganic composites of chitosan and hydroxyapatite were obtained by preparing a joint solution and pouring onto a glass substrate. According to XRD and DTA data, addition of hydroxyapatite reduces the softening and decomposition temperature of chitosan. This information is necessary to optimize the manufacturing processes of organo–inorganic scaffolds.

The aim of the study is to evaluate biocompatibility of a novel hybrid polyoligomer in in vitro and in vivo models. Materials and Methods Cytotoxicity of the material was investigated using the MTT assay with human dermal fibroblasts as test cultures. To study direct interaction of the hybrid polyoligomer with cells, the fibroblasts were cultured on the polymer samples for 96 h, the cultures were assessed every 24 h using fluorescence microscopy. To study the tissue reaction in the area of contact with the donor bed and the morphological features of the implanted sample restructuring, a case-control study was performed using a rabbit model. Samples of hybrid polyoligomer were implanted into the bone defect formed in the left iliac crest in 10 rabbits. In the control group, the prepared allograft samples were transplanted into similar defects in 10 animals. The rabbits were sacrificed 4 and 8 weeks after the operation. The standard morphological methods with hematoxylin and eosin staining and immunohistochemical Ki-67 proliferation marker evaluation were used to assess the state of tissues in the defect area. Results The results demonstrate that the hybrid polyoligomer is not cytotoxic (cytotoxicity score 0-1), cells adhere well to its surface, retain their viability and typical morphology throughout the entire observation period. No negative impact of material implantation on the health state and behavior of animals was detected. Morphological examination showed the absence of inflammatory changes, formation of thin-walled capillary vessels, and considerable proliferative activity of mesenchymal cells in the defect area, even though it was more intense than in the control group. Conclusion No inflammation signs were detected by 8th week of the experiment. It was defined that new bone was beginning to form. The results of analysis support the conclusion that the developed hybrid materials are prospective for further research as potential bone substitute.