1. Terahertz dielectric assay of solution phase protein binding.
- Author
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Chen, Jing-Yin, Knab, J. R., Ye, Shuji, He, Yunfen, and Markelz, A. G.
- Subjects
- *
SPECTRUM analysis , *BIOCHEMISTRY , *LIGAND binding (Biochemistry) , *LIGANDS (Chemistry) , *QUALITATIVE chemical analysis - Abstract
The authors demonstrate a method for rapid determination of protein-ligand binding on solution phase samples using terahertz dielectric spectroscopy. Measurements were performed using terahertz time domain spectroscopy on aqueous solutions below the liquid-solid transition for water. Small ligand binding sensitivity was demonstrated using triacetylglucosamine and hen egg white lysozyme with a decrease in dielectric response with binding. The magnitude of the change increases with frequency. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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