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Microbial infections are a general public health problem worldwide, and their resistance to antibiotics has increased rapidly. The prevention of microbial growth on the surface of cotton fabric is essential to control skin-related infections. In this work, we evaluated A.calamus- zinc oxide nanoparticle (AC-ZnONPs) coated cotton fabrics for antibacterial and cytotoxic activities. The formulated AC-ZnONPs were characterized by UV-spectrophotometer, SEM, EDX, FT-IR, and TEM studies. The AC-ZnONPs amalgamation into the cotton fabrics was done by the dipping technique. The antimicrobial activity and cytotoxic activity were executed by standard approaches. The results of UV-spectrophotometer, TEM, and SEM, and EDX proved the formation and existence of AC-ZnONPs on the fabrics' surface. FT-IR results demonstrated the existence of numerous functional groups. AC-ZnONPs efficiently suppressed the growth of the pathogenic microbes with a maximum inhibition at 60 μg of AC-ZnONPs against E.coli and S.aureus. The AC-ZnONPs coated fabrics also prevented microbial growth with maximum inhibition against P.aeruginosa, K.oxytoca, and A.baumanii. In addition, AC-ZnONPs efficiently suppressed the viability of SK-MEL-3 cells. Altogether, our findings disclosed that the AC-ZnONPs coated fabrics displayed excellent antimicrobial activity against the pathogenic microbes and could be utilized in the medical fields in the future.

The majority of industrial products are identified as persistent organic pollutants after their date of expiry, which is highly harmful to the ecosystem and human health and also going to be banned around the world. Paint latex is one of those pollutants which become a hazardous waste material after stocking for a long time. Approximately 20% of color paints do not get used for their desired purpose after getting sold out and end up in a landfill. Now a day's construction industry is inclining towards the various types of geo-polymer concrete since it does not require cement. But that geo-polymer concrete has too much less workability as compared to the same grade of control cement concrete. To achieve the desired workability as well as other properties of geo-polymer concrete by using waste paint latex as performance improving admixture is the main motive of the present research. Fourteen different mixes of control and calcined clay-based geo-polymer concrete have been prepared by adding up to 3% waste paint latex of weight of cementitious materials and a detailed study has been done on various properties such as workability, rheology, shrinkage, strength and its microstructure. The presence of nanoparticles of TiO

Candida infections and related mortality have become a challenge to global health. Nontoxic and natural bioactive compounds from plants are regarded as promising candidates to inhibit these multidrug resistant strains. In the present study, in vitro assays and in silico molecular docking approach was combined to evaluate the inhibitory effect of crude extracts from Allium ampeloprasum and its variety A. porrum on Candida pathogens. Phytochemical screening revealed the presence of phenolic acids and flavonoids in higher quantity. Spectral studies of the extracts support the presence of phenols, flavonoids and organosulfur compounds. Aqueous extract of A. ampeloprasum showed a total antioxidant capacity of 68 ± 1.7 mg AAE/ g and an IC50 value of 0.88 ± 2.1 mg/ml was obtained for DPPH radicals scavenging assay. C. albicans were highly susceptible (19.9 ± 1.1 mm) when treated with aqueous A. ampeloprasum extract. Minimum inhibitory concentrations were within the range of 19–40 μg/ml and the results were significant (p ≤ 0.05). In silico molecular docking studies demonstrated that bioactive phytocompounds of A. ampeloprasum and A. porrum efficiently interacted with the active site of Secreted aspartyl proteinase 2 enzyme that is responsible for the virulence of pathogenic yeasts. Rosmarinic acid and Myricetin exhibited low binding energies and higher number of hydrogen bond interactions with the protein target. Thus the study concludes that A. ampeloprasum and A. porrum that remain as underutilized vegetables in the Allium genus are potential anti-candida agents and their pharmacologically active compounds must be considered as competent candidates for drug discovery.

Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability. In this investigation, magnetic cores (Fe3O4) were synthesized using a fabrication method involving coprecipitation of Fe2+ and Fe3+. Then the magnetic nanoparticles were encapsulated by chitosan layers. In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent. The morphological and physicochemical features of the material were determined using several advanced techniques like FT-IR, ICP analysis, FESEM, EDS, XRD, TEM, XPS and VSM. In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2. The IC50 of Fe3O4@CS/AuNPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, HT-29, MKN45, AGS, KATO III, PANC-1, AsPC-1, and MIA PaCa-2 cell lines were 385, 429, 264, 286, 442, 498, 561, 513, 528, and 425 μg/mL, respectively. Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line. Seemingly, the present nanoparticles may be used for the treatment of several types of gastro-duodenal cancers especially colon, gastric, and pancreatic cancers in near future.

In the current research, AA6082 aluminium alloy matrix composites (AAMCs) incorporated with various weight fractions of titanium diboride (0, 3, 6, and 9 wt%) were prepared via an in situ casting technique. The exothermic reaction between inorganic powders like dipotassium hexafluorotitanate (K2TiF6) and potassium tetrafluoroborate (KBF4) in molten Al metal contributes to the development of titanium diboride content. The manufactured AA6082-TiB2 AAMCs were evaluated using a scanning electron microscope (SEM) and X-ray diffraction (XRD). The mechanical properties and wear rate (WR) of the AAMCs were investigated. XRD guarantees the creation of TiB2 phases and proves the nonappearance of reaction products in the AMCs. SEM studies depict the even dispersion of TiB2 in the matrix alloy. The mechanical and tribological properties (MTP) of the AAMCs showed improvement by the dispersion of TiB2 particles. The WR decreases steadily with TiB2 and the least WR is seen at nine weight concentrations of TiB2/AA6082 AAMCs. Fabricated composites revealed 47.9% higher flexural strength and 14.2% superior compression strength than the base AA6082 alloy.

Zerovalent Iron Nanoparticles (MC-ZVI NPs) were synthesized from Musa coocinea peel extract as reducing and stabilizing agent using a novel synthesis technique. The synthesis of MC-ZVI NPs was confirmed using UV-vis spectroscopy showing a sharp absorption peak at 341 nm. Further the chemical and structural characterization of MC-ZVI NPs were performed using Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Dynamic Light Scattering technique (DLS). FTIR analysis revealed the presence of phytochemical molecules associated with the MC-ZVI NPs. SEM analysis revealed the synthesized MC-ZVI NPs were in spherical shaped, while DLS analysis confirmed the synthesis of poly dispersed and non-homogenous MC-ZVI NPs. The antimicrobial efficacy of MC-ZVI NPs synthesized using Musa coccinea peel extract was tested against bacterial (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus subtilis) and fungal (Aspergillus niger) pathogens. But MC-ZVI NPs exhibited maximum of 19 mm zone of inhibition against B. subtilis and A. niger. Further the free radical scavenging activity MC-ZVI NPs was confirmed using DPPH, hydroxyl radical, hydrogen peroxide, FRAP assay showing displayed effective antioxidant activity. Thus, the present idea will give a fast and cost effective approach to synthesize MC-ZVI NPs with antimicrobial property for application in biomedical purposes.

Leukemia is amongst the cancers accountable for substantial mortality around the world. Tomentosin is a bioactive compound with a pharmacological significance, and its anticancer property against human leukemia MOLT-4 cell line has never been reported. Hence, the objective of this study was to explore the anticancer activity of tomentosin in MOLT-4 human leukemia cells. In the current investigation, the cytotoxic effects of tomentosin ensuing potent toxicity (IC50 : 10 µM) in MOLT-4 cells after incubation at 24 h have been presented. Furthermore, tomentosin triggered intracellular reactive oxygen species production and showed the induction of intrinsic/mitochondrial pathways in treated MOLT-4 cells, revealing a significant cytotoxicity activity. Also, fluorescent microscopic studies using acridine orange/ethidium bromide and propidium iodide staining confirmed the occurrence of apoptosis in tomentosin-treated MOLT-4 cells. Quantitative reverse transcription polymerase chain reaction presented a negative regulation of cyclin D1 and BcL-2 expression and a positive regulated BAX and caspase-3 messenger RNA expression in tomentosin-treated MOLT-4 cells. Tomentosin further inhibited the inflammatory transcription factors such as nuclear factor κB (NF-κB), tumor necrosis factor α, interleukin 1β (IL-1β), and IL-6. Additionally, inhibition of the m-TOR/PI3K/AKT protein expression by tomentosin in MOLT-4 cells was confirmed. Overall, these findings lead to a conclusion that tomentosin induces apoptosis in MOLT-4 cells through caspase-facilitated proapoptotic pathway, and inhibition of the NF-κB-stimulated Bcl-2 facilitated the antiapoptotic pathway.