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Your search keyword '"Gibran Nasir"' showing total 3 results
Start Over Author "Gibran Nasir" Topic biochemistry
3 results on '"Gibran Nasir"'

1. A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids

Author

Ximena Steinberg, Jason Galpin, Gibran Nasir, Romina V. Sepúlveda, Ernesto Ladron de Guevara, Fernando Gonzalez-Nilo, Leon D. Islas, Christopher A. Ahern, and Sebastian E. Brauchi

Subjects
Bioorganic chemistry, Bioinformatics, Proteins, Biochemistry, Molecular biology, Unnatural amino acids, Science (General), Q1-390, Social sciences (General), H1-99
Abstract

The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids (f-ncAA) have been successfully incorporated in proteins expressed in bacterial systems, Xenopus oocytes, and HEK-293T cells. Here, we describe the rational generation of a novel orthogonal aminoacyl-tRNA synthetase based on the E. coli tyrosine synthetase that is capable of encoding the f-ncAA tyr-coumarin in HEK-293T cells.

Published
2020
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2. A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids

Author

Christopher A. Ahern, Ernesto Ladron de Guevara, Romina V. Sepúlveda, Gibran Nasir, León D. Islas, Sebastian Brauchi, Jason D. Galpin, Ximena Steinberg, and Fernando D. González-Nilo

Subjects
0301 basic medicine, Bioinformatics, Molecular biology, Xenopus, Coumarin, Biochemistry, Fluorescence, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, In vivo, Bioorganic chemistry, lcsh:Social sciences (General), Tyrosine, lcsh:Science (General), chemistry.chemical_classification, Multidisciplinary, biology, Chemistry, Aminoacyl tRNA synthetase, Proteins, biology.organism_classification, In vitro, Amino acid, 030104 developmental biology, Aminoacyl-tRNA synthetase, lcsh:H1-99, Unnatural amino acids, 030217 neurology & neurosurgery, lcsh:Q1-390, Research Article
Abstract

The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids (f-ncAA) have been successfully incorporated in proteins expressed in bacterial systems, Xenopus oocytes, and HEK-293T cells. Here, we describe the rational generation of a novel orthogonal aminoacyl-tRNA synthetase based on the E. coli tyrosine synthetase that is capable of encoding the f-ncAA tyr-coumarin in HEK-293T cells., Bioorganic Chemistry; Bioinformatics; Proteins; Biochemistry; Molecular Biology; Unnatural amino acids, aminoacyl-tRNA synthetase, coumarin, fluorescence.

Published
2020

3. A Rationally Designed Aminoacyl-tRNA Synthetase for Genetically Encoded Fluorescent Amino Acids

Author

Romina V. Sepúlveda, Christopher A. Ahern, Ximena Steinberg, Sepulveda-Ugarte J, Sebastian Brauchi, Gibran Nasir, Fernando D. González-Nilo, León D. Islas, and Jason D. Galpin

Subjects
chemistry.chemical_classification, 0303 health sciences, biology, Aminoacyl tRNA synthetase, Xenopus, 010402 general chemistry, biology.organism_classification, 01 natural sciences, Fluorescence, In vitro, 3. Good health, 0104 chemical sciences, Amino acid, 03 medical and health sciences, chemistry.chemical_compound, chemistry, Biochemistry, In vivo, Tyrosine, 030304 developmental biology
Abstract

The incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids (f-ncAA) have been successfully incorporated in proteins expressed in bacterial systems, Xenopus oocytes, and HEK-293T cells. Here, we describe the rational generation of an orthogonal aminoacyltRNA synthetase based on the E. coli tyrosine synthetase that is capable of encoding the f-ncAA tyr-coumarin in HEK-293T cells.

Published
2016

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