1. Functionally relevant neutrophilia in CD11c diphtheria toxin receptor transgenic mice
- Author
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Günter J. Hämmerling, Chrystel Llanto, Daniel R. Engel, Christina Ohliger, André P. Tittel, Christoph Heuser, Natalio Garbi, Christian Kurts, and Simon Yona
- Subjects
Male ,Genetically modified mouse ,Neutrophils ,Transgene ,CD11c ,Mice, Transgenic ,Inflammation ,Biology ,Biochemistry ,Granulopoiesis ,Mice ,medicine ,Animals ,Uropathogenic Escherichia coli ,Diphtheria Toxin ,Interleukin 8 ,Molecular Biology ,Mice, Inbred BALB C ,Pyelonephritis ,Macrophages ,Models, Immunological ,hemic and immune systems ,Dendritic Cells ,Cell Biology ,Dendritic cell ,Molecular biology ,Neutrophilia ,CD11c Antigen ,Mice, Inbred C57BL ,Disease Models, Animal ,Immunology ,Female ,medicine.symptom ,Biotechnology - Abstract
Transgenic mice expressing the diphtheria toxin receptor (DTR) in specific cell types are key tools for functional studies in several biological systems. B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J (CD11c.DTR) and B6.Cg-Tg(Itgax-DTR/OVA/EGFP)1Gjh/Crl (CD11c.DOG) mice express the DTR in CD11c(+) cells, allowing conditional depletion of dendritic cells. We report that dendritic-cell depletion in these models caused polymorphonuclear neutrophil (PMN) release from the bone marrow, which caused chemokine-dependent neutrophilia after 6-24 h and increased bacterial clearance in a mouse pyelonephritis model. We present a transgenic mouse line, B6.Cg-Tg(Itgax-EGFP-CRE-DTR-LUC)2Gjh/Crl (CD11c.LuciDTR), which is unaffected by early neutrophilia. However, CD11c.LuciDTR and CD11c.DTR mice showed late neutrophilia 72 h after dendritic cell depletion, which was independent of PMN release and possibly resulted from increased granulopoiesis. Thus, the time point of dendritic cell depletion and the choice of DTR transgenic mouse line must be considered in experimental settings where neutrophils may be involved.
- Published
- 2012
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