7 results on '"Wang, Beilei"'
Search Results
2. Preparation, characterization and preliminary pharmacokinetic study of pH‐sensitive Hydroxyapatite/Zein nano‐drug delivery system for doxorubicin hydrochloride.
- Author
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Zha, Liqiong, Wang, Beilei, Qian, Jiajia, Fletcher, Brock, Zhang, Caiyun, Dong, Qiannian, Chen, Weidong, and Hong, Lufeng
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DOXORUBICIN , *ANTHRACYCLINES , *HYDROXYAPATITE , *TRANSMISSION electron microscopes , *DRUG delivery systems , *ZETA potential , *INFRARED spectroscopy - Abstract
Objectives: Zein nanoparticles (Zein NPs) were used as a hydroxyapatite (HA) biomineralization template to generate HA/Zein NPs. Doxorubicin hydrochloride (DOX) was loaded on HA/Zein NPs (HA/Zein‐DOX NPs) to improve its pH‐sensitive release, bioavailability and decrease cardiotoxicity. Methods: HA/Zein‐DOX NPs were prepared by phase separation and biomimetic mineralization method. Particle size, polydispersity index (PDI), Zeta potential, transmission electron microscope, X‐ray diffraction and Fourier‐transform infrared spectroscopy of HA/Zein‐DOX NPs were characterized. The nanoparticles were then evaluated in vitro and in vivo. Key findings: The small PDI and high Zeta potential demonstrated that HA/Zein‐DOX NPs were a stable and homogeneous dispersed system and that HA was mineralized on Zein‐DOX NPs. HA/Zein‐DOX NPs showed pH‐sensitive release. Compared with free DOX, HA/Zein‐DOX NPs increased cellular uptake which caused 7 times higher in‐vitro cytotoxicity in 4T1 cells. Pharmacokinetic experiments indicated the t1/2β and AUC0–t of HA/Zein‐DOX NPs were 2.73‐ and 3.12‐fold higher than those of DOX solution, respectively. Tissue distribution exhibited HA/Zein‐DOX NPs reduced heart toxicity with lower heart targeting efficiency (18.58%) than that of DOX solution (37.62%). Conclusion: In this study, HA/Zein‐DOX NPs represented an antitumour drug delivery system for DOX in clinical tumour therapy with improved bioavailability and decreased cardiotoxicity. [ABSTRACT FROM AUTHOR]
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- 2020
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3. Preparation, preliminary pharmacokinetics and brain tissue distribution of Tanshinone IIA and Tetramethylpyrazine composite nanoemulsions.
- Author
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Wang, Beilei, Hong, Lufeng, Liu, Yuanxu, Bedingfield, Sean K., Zhang, Caiyun, Peng, Can, Qian, Jiajia, and Zha, Liqiong
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PHARMACOKINETICS ,TISSUES ,BIOAVAILABILITY ,NANOPARTICLES - Abstract
Objective: Tanshinone IIA (TSN) and Tetramethylpyrazine (TMP) were combined in a composite, oil-in-water nanoemulsions (TSN/TMP O/W NEs) was prepared to prolong in vitro and vivo circulation time, and enhance the bioavailability of TSN. Material and methods: Physicochemical characterization of TSN/TMP O/W NEs was characterized systematically. The in vitro dissolution and in vivo pharmacokinetic experiments of TSN/TMP O/W NEs were also evaluated. Result: A formulation was optimized, yielding a 32.5 nm average particle size, an encapsulation efficiency of over 95 %, and were spherical in shape as shown by TEM. TSN/TMP O/W NEs were shown to extend the release and availability in vitro compared to raw compounds. In pharmacokinetic study, the AUC
0→∞ and t1/2 of the TSN/TMP O/W NEs were 481.50 mg/L*min and 346.39 min higher than TSN solution, respectively. Brain tissue concentration of TSN was enhanced with TSN/TMP O/W NEs over raw TSN and even TSN O/W NEs. Conclusions: Therefore, nanoemulsions are an effective carrier to increase encapsulation efficiency of drugs, improve bioavailability and brain penetration for TSN – which is further enhanced by pairing with the co-delivery of TMP, providing a promising drug delivery. [ABSTRACT FROM AUTHOR]- Published
- 2019
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4. Preparation and preliminary pharmacokinetics study of GNA‐loaded zein nanoparticles.
- Author
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Cheng, Weiye, Wang, Beilei, Zhang, Caiyun, Dong, Qiannian, Qian, Jiajia, Zha, Liqiong, Chen, Weidong, and Hong, Lufeng
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ZEIN (Plant protein) , *BIOAVAILABILITY , *NANOCAPSULES , *NANOCARRIERS , *DRUG delivery systems , *NANOPARTICLES - Abstract
Objectives: Gambogenic acid (GNA), one of the main active ingredients isolated from Garcinia cambogia, has shown diverse antitumour activities. However, short biological half‐life and low oral bioavailability severely limit its clinical application. Here, we developed GNA‐loaded zein nanoparticles (GNA‐ZN‐NPs) based on phospholipid complex and zein nanoparticles to prolong the circulation time and enhance oral bioavailability of GNA. Methods: The physicochemical properties of GNA‐ZN‐NP were characterized in details. The in vitro release profile, in vivo pharmacokinetic experiments and tissue distribution of GNA‐ZN‐NPs were also evaluated. Key findings: The particle size, PDI and encapsulation efficiency of GNA‐ZN‐NPs were 102.90 nm, 0.027 and 76.35 ± 0.64%, respectively. The results of SEM, FTIR, DSC and XRD demonstrated that GNA‐ZN‐NPs were prepared successfully. The in vitro dissolution of GNA‐ZN‐NPs exhibited controlled release compared with raw GNA solution. The pharmacokinetic study showed that the AUC of GNA‐ZN‐NPs was significantly increased, and the t1/2 and MRT values of GNA‐ZN‐NPs were 3.21‐fold and 2.19‐fold higher than that of GNA solution. Tissue distribution results illustrated that GNA‐ZN‐NPs showed hepatic‐targeting properties. Conclusion: GNA‐ZN‐NPs significantly enhanced the oral bioavailability and prolonged half‐life of GNA, providing a promising oral drug delivery system to improve in vivo pharmacokinetic behaviour of GNA. [ABSTRACT FROM AUTHOR]
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- 2019
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5. Synthesis, cytotoxicity and liver targeting of 3‐O‐β‐D‐Galactosylated Resveratrol.
- Author
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Qian, Jiajia, Zha, Liqiong, Wang, Beilei, Zhang, Caiyun, Hong, Lufeng, and Chen, Weidong
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CELL-mediated cytotoxicity ,RESVERATROL ,POLYPHENOLS ,LIVER diseases ,PHARMACOKINETICS - Abstract
Objectives: Resveratrol (Res), a naturally occurring polyphenol, has shown pharmacological activities in treatment of liver diseases. However, the application of Res was limited by its poor bioavailability and liver targeting. Herein, 3‐O‐β‐D‐Galactosylated Resveratrol (Gal‐Res) was synthesized by structural modification of Res to enhance bioavailability and liver targeting. Methods: The Gal‐Res was characterized by IR, 1H‐NMR spectra and MS. The in vitro antitumour experiments, in vivo pharmacokinetics and biodistribution studies were evaluated. Results: Gal‐Res was successfully synthesized in our study. Compared to Res, Gal‐Res resulted in enhanced cytotoxicity in HepG2 cells. After intravenous injection of normal SD rats, Gal‐Res significantly improved the bioavailability of Res and the Cmax and AUC0–t of Gal‐Res were 3.186 and 3.929 time than that of Res. In addition, in the study of liver targeting, the relative uptake rate (Re) of Gal‐Res in the liver (2.006) is the largest. The drug targeting efficiency (Te; 38.924%) of Gal‐Res was greater than that of Res. These showed that Gal‐Res could significantly improve the distribution ability of Res in liver. Conclusions: On the whole, Gal‐Res increased cellular uptake to HepG2 cells, bioavailability and liver targeting, providing its future clinical application in the treatment of liver diseases. [ABSTRACT FROM AUTHOR]
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- 2019
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6. Effect of humin modified by Fe(NO3)3/FeSO4 on the bioavailability of vanadium in Panzhihua mining: characteristics and mechanisms.
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Li, Peirou, Deng, Siwei, Wu, Yuerong, Li, Sijia, Huangfu, Zhuoxi, Sun, Xiaoshuang, and Yu, Jiang
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PEAT soils ,THERAPEUTIC immobilization ,BIOAVAILABILITY ,TITANIUM ,VANADIUM ,BIOCHAR - Abstract
Panzhihua, China, ranks third in the world in vanadium (V) and titanium (Ti) resources reserves. Due to the development and utilization of vanadium, vanadium enters the surrounding soil and groundwater, affecting the ecological environment and human health. In this study, humin (HM) was extracted from peat soil and modified with iron-based materials to get Fe-HM (Fe(NO
3 )3 and FeSO4 ). The properties of Fe-HM characterized by EA, NMR, SEM, EDS, BET, and FTIR showed larger specific surfaces and more oxygen-containing groups. Then, Fe-HM was applied to V-contaminated soil to investigate its immobilization effect. Applying HM/Fe-HM to vanadium-contaminated soils can alter soil properties, reduce the bioavailability of vanadium (23.5% to 21.0%(HM), 17.0% (Fe(NO3 )3 -HM), and 17.7%(FeSO4 -HM)), and improve immobilization efficiency (40.0%(HM), 79.2%(Fe(NO3 )3 -HM), and 75.9%(FeSO4 -HM)), thus achieving the aim of remediating vanadium-contaminated soils and reducing the risk of vanadium to the environment. [ABSTRACT FROM AUTHOR]- Published
- 2023
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7. In vitro/in vivo evaluation of pH-sensitive Gambogenic acid loaded Zein nanoparticles with polydopamine coating.
- Author
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Zha, Liqiong, Qian, Jiajia, Wang, Beilei, Liu, Huanhuan, Zhang, Caiyun, Dong, Qiannian, Chen, Weidong, and Hong, Lufeng
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NANOPARTICLES , *DOPAMINE , *ZETA potential , *BLOOD vessels , *SURFACE coatings , *Z bosons - Abstract
Schematic illustration of the preparation, pH-sensitive release, cellular uptake and concentration–time profile of GNA@Zein-PDA NPs. As one of the active pharmaceutical ingredients in Gamboge, Gambogenic acid (GNA) has shown diverse anti-tumor activities. To reduce the vascular irritation of GNA and improve its water solubility, tumor targeting, and bioavailability, GNA loaded Zein nanoparticles (GNA@Zein NPs) was further coated by polydopamine (PDA) to develop GNA@Zein-PDA NPs by anti-solvent precipitation and surface modification. The results showed that particle size and Zeta potential of GNA@Zein-PDA NPs were about 310 nm and −40.8 mV with core–shell morphology confirmed by TEM. GNA@Zein-PDA NPs increased the water solubility of GNA by more than 700 times and showed pH-sensitive release behavior in PBS with pH 6.86. In vitro cytotoxicity tests showed that GNA@Zein-PDA NPs had higher inhibitory activity on HepG2 cells than free GNA, and their IC 50 were 1.59 μg/mL and 9.89 μg/mL, respectively. Additionally, the hemolysis and vascular irritation assay showed that GNA@Zein-PDA NPs had good cytocompatibility and reduced the irritation of GNA to blood vessels. Moreover, the in vivo pharmacokinetic experiments exhibited that the C max and AUC 0-t of GNA@Zein-PDA NPs were significantly improved approximately by 2.09-fold and 3.48-fold over that of GNA, respectively. In conclusion, GNA@Zein-PDA NPs solve many defects of GNA and provide a tumor-targeting drug delivery for GNA. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
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