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• Protein concentrate production conditions were optimized. • Genotype effected the features of protein concentrates. • Some genotypes exhibited superior technological properties. • Color effected the bioactive features. In this study, a comparative investigation on the structural, technofunctional and bioactive properties of protein concentrates from different common bean genotypes was performed. Protein extractions were carried out at different pH and salt concentrations and the highest protein content for the concentrates (77.7%) was determined for pH 11 and 0.4% of salt. The protein content of the common bean flour and their protein concentrates was in the range of 22–26.93% and 72.97–77.99%, respectively. For bioactive properties, total phenolic content ranged between 578.9 and 1355.9 and 313.5–1219.1 mg GAE/kg, for bean flours and protein concentrates, respectively. Two genotypes (G7 and G8) were the samples showing the superior biofunctional properties compared to the others. Thermal characterization showed that T d and ΔH values were in the range of 64.95–94.33 °C and 76.64–122.3 j/g, respectively. SDS-PAGE analysis revealed that the major band corresponded to the 7S vicilin. Principal component analysis showed that G2 and G6 had different characteristics in terms of technofunctional parameters while G7 and G8 were differed from the other genotypes in terms of bioactivity. The results showed that the proteins of common beans could be evaluated as good source due to high bioactivity for the enrichment of food formulations. [ABSTRACT FROM AUTHOR]

In this study, aerial parts of dandelion were exposed to extraction by different solvents such as water, ethanol, methanol and glycerol and also their aqueous mixtures to compare the effect of extraction solvents on bioactive performance of the dandelion and also to show the effectiveness of hydroglycerolic extraction which is a green extraction process. Total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were determined and also antiradical scavenging activities and antioxidant capacities of the samples were also evaluated. TPC and TFC of the samples ranged between 4.63-21.28 mg GAE/g and 1.16-14.38 mg CE/g, respectively. The highest TPC and TFC values were determined in aqueous extract of glycerol (75% w/w) compared to other solvents. Additionally, ABTS.+ and DPPH radical scavenging activity and ferric reducing capacity and antioxidant capacity values were determined for the extracts and the best solvent was also aqueous glycerol (75% w/w). [ABSTRACT FROM AUTHOR]

• Capsicum annuum (Long pepper) × Capsicum frutescens (PI281420) were selfed. • Total phenolics, flavonoid and total anthocyanin contents of the red pepper lines changed significantly. • Scoville heat unit revealed that the 95% of the peppers were highly pungent. • The major phenolics of peppers were detected as p-coumaric acid. In this study, 104 RIL (Recombinant Inbred Pepper Lines: F6) populations which generated by selfing Capsicum annuum (Long pepper) × Capsicum frutescens (PI281420) F6 population were characterized in terms of detailed bioactive properties, major phenolic composition, tocopherol and capsaicinoid profile. Total phenolics, flavonoid and total anthocyanin contents of the red pepper lines were in the range of 7.06–17.15 mg gallic acid equivalent (GAE)/g dw, 1.10–5.46 mg catechin equivalent (CE)/g dw and 7.9–516.6 mg/kg dw extract, respectively. Antiradical activity and antioxidant capacity values also ranged between 18.99 and 49.73% and 6.97–16.47 mg ascorbic acid equivalent (AAE)/kg dw, respectively. Capsaicin and dihydrocapsaicin levels showed a wide variance with the range of 27.9–1405.9 and 12.3–640.4 mg/100 g dw, respectively. Scoville heat unit revealed that the 95% of the peppers were highly pungent. The major tocopherol was alpha tocopherol for the pepper samples with the highest level of 1078.4 µg/g dw. The major phenolics were detected as p-coumaric acid, ferulic acid, myricetin, luteolin and quercetin. Pepper genotypes showed significant differences in terms of the characterized properties and principal component analysis was applied successfully to reveal the similar genotypes. [ABSTRACT FROM AUTHOR]

In this study, gallic acid (GA) which is one of the most popular phenolics was encapsulated using yeast cell (S. cerevisiae) and effects of plasmolysis treatment and solvent type of encapsulation media (H 2 O or EtOH:H 2 O) on encapsulation performance, bioactive properties, morphological-conformational characteristics and release behavior at simulated gastric and intestine medium (pH 1.2 and 6.8) were investigated. Encapsulation efficiency (EE) was determined to be significantly higher in nonplasmolysed yeast cells treated with H 2 O (NPYC–H 2 O). GA concentration of microcapsules ranged between 6.48 and 94.76 mg GAE/g sample and the microcapsules showed antioxidant and antiradical activity. It was revealed that the solvent type of encapsulation media showed significant effect on the EE and studied bioactive parameters. GA released from yeast cells efficiently in both pH 1.2 and 6.8, more than 80% after 2 h of release and the releasing behavior was explained by Korsmeyer-Peppas kinetic model by high fitting performance. It was concluded that GA could be encapsulated by nonplasmolysed yeast cell in aqueous media effectively. • Yeast cell was used as a microcarrier for gallic acid. • Plasmolysis treatment caused a decrease in encapsulation efficiency. • Solvent type of encapsulation media showed significant effect on microcapsule properties. • Gallic acid release was explained by Korsmeyer-Peppas kinetic model. [ABSTRACT FROM AUTHOR]

Hypericum perforatum is a widely used medicinal plant known that it has high bioactive performance due to its special biochemical compounds in its structure. The current study aimed to determine the optimum processing parameters of extraction conditions of bioactive constituents in H. perforatum by ultrasound application and its some bioactive performance. For this purpose, different processing variables namely sonication period (min), liquid/solid ratio and ethanol concentration (%) were selected to characterize the best conditions for extraction of H. perforatum. The statistical analysis results revealed that the most effective factor on the studied parameters was ethanol concentration and increment of ethanol level increased the characterized responses until a constant value. The constructed regression models fit very well with quite high determination of coefficients (R²>0.906). Total phenolic content was in the range of 39.54-63.55 mg GAE/g sample while total hypericin concentration ranged between 211.5-858.7 mg/kg. Multiple response optimization results revealed that the optimum extraction conditions would be at 47.6% ethanol concentration and 22.2 mL of liquid/solid ratio for 60 min ultrasonic process application. The results revealed that the optimized extraction conditions could be utilized for ultrasound extraction system by industrial application. [ABSTRACT FROM AUTHOR]

Thirty-three different Hypericum perforatum genotypes were characterized in terms of morphological (plant height, herbage yields), physicochemical (ash, cellulose, protein, and colour), and bioactive properties. The antioxidant and antibacterial capacity were also assessed. All genotype samples were characterized as flower part and whole plant (aerial part). It was observed that the plant height ranged between 21.6 and 96.6 cm and whole plant drog yield was in the range of 11–146 g. Bioactivity showed wide distribution for the samples and all bioactive parameter values were measured as lower for the whole plant compared to the flower part. Total phenolic content ranged between 60.39-110.54 and 49.08–89.53 mg GAE/g for the flower part and whole plant, respectively. Flower extracts also showed good inhibition activity against some Gram (+) bacteria. The GT-Biplot statistical mathematical classification approach was applied to reveal the promising genotypes. • Hypericum perforatum genotypes were morphologically investigated. • The antioxidant and antibacterial capacity were also assessed. • Extracts from flower parts showed higher bioactivity than those from whole plants. • Flower extracts inhibited some Gram (+) bacteria. • GT-Biplot approach was applied to unravel the promising genotypes. [ABSTRACT FROM AUTHOR]