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Your search keyword '"C. Procaccianti"' showing total 3 results
Start Over Author "C. Procaccianti" Topic bio/06 - anatomia comparata e citologia
3 results on '"C. Procaccianti"'

1. Whole genome and microRNA analysis in a human hepatoma cell line exposed to Cd

Author

C. Procaccianti, M.G. Saccoa, Chiara Urani, Marco Fabbri, Laura Gribaldo, Gribaldo, L, Fabbri, M, Sacco, M, Procaccianti, C, and Urani, C

Subjects
Genetics, Hepatoma cell line, cadmium, whole genome analysis, microRNA, carcinogenesis, environmental contamination, microRNA, General Medicine, Computational biology, Biology, Toxicology, Genome, BIO/06 - ANATOMIA COMPARATA E CITOLOGIA
Published
2011

2. The cell trasformation assay: Toward a statistical classification of mixed and intermediate foci images

Author

Federico M. Stefanini, C. Procaccianti, Chiara Urani, Procaccianti, C, Stefanini, F, and Urani, C

Subjects
quantitative morphology, Focus (geometry), Computer science, Carcinogenicity Tests, Quantitative morphology, Toxicology, Animal Testing Alternatives, General Biochemistry, Genetics and Molecular Biology, Mice, in vitro cell trasformation, Image Processing, Computer-Assisted, cluster analysi, Animals, Cluster Analysis, Neoplastic transformation, Statistical descriptors, BIO/06 - ANATOMIA COMPARATA E CITOLOGIA, Alternative methods, Microscopy, business.industry, Pattern recognition, General Medicine, chemical carcinogenesi, Medical Laboratory Technology, Statistical classification, Transformation (function), Cell Transformation, Neoplastic, Artificial intelligence, business
Abstract

The human carcinogenicity evaluation of chemicals has a great impact on public health. In vitro methods, such as the cell transformation assay (CTA), allow for a fast and reliable assessment of the carcinogenic potential of a chemical compound in comparison with the standard two-year bioassay. The scoring and classification of foci in selected cell lines is performed, after staining, by light microscopy. Foci can be separated into three classes: type I, which are scored as non-transformed, and types II and III that are considered to include fully transformed foci. However, in a number of cases, even an expert is uncertain about the attribution of a focus to a given class, due to its mixed or intermediate nature. Here, we suggest a simple approach to classifying mixed or intermediate foci by exploiting the quantitative information available from images, which is captured by statistical descriptors. A quantitative index is proposed, to describe the degree of dissimilarity of mixed and intermediate images to the three well-distinguished classes.

Published
2011

3. Whole genome analysis and micrornas regulation in HepG2 cells exposed to cadmium

Author

C. Procaccianti, Maria Grazia Sacco, Chiara Urani, Marco Fabbri, Laura Gribaldo, Fabbri, M, Urani, C, Sacco, M, Procaccianti, C, and Gribaldo, L

Subjects
Pharmacology, cadmium, gene ontology, HepG2 cells, pathway mapping, toxicogenomics, Principal Component Analysis, Genome, Microarray, Gene Expression Profiling, General Medicine, Hep G2 Cells, Biology, Bioinformatics, Cell biology, Gene expression profiling, Medical Laboratory Technology, MicroRNAs, Gene Expression Regulation, Gene expression, microRNA, Hepatocytes, Metallothionein, Humans, Liver function, Gene, Carcinogen, BIO/06 - ANATOMIA COMPARATA E CITOLOGIA, Cadmium
Abstract

Cadmium (Cd) is a metal known to be toxic and carcinogenic, but its mechanism of action remains to be fully elucidated. We investigated the gene expression modulation in the human hepatoma cell line HepG2 after exposure to 2 μM and 10 μM Cd using an Agilent microarray. Furthermore, we evaluated the microRNA modulation after exposure to 10 μM Cd with a Low Density Array. At the low concentration only eleven genes belonging to the metallothionein familiy were regulated. At the higher concentration the pathway enrichment analysis for the 536 up-regulated genes showed a large number of pathways related to cancer, whereas the 424 down-regulated genes were enriched on pathways correlated to liver function. A large percentage of modified microRNAs belonged to the let-7 family, which is considered to have oncosuppressor functions. Several pathways connected to cancer were regulated at the transcription level, and miRNAs had a potential impact on the modulation of this regulation.

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