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[SPA] Los consumidores demandan asiduamente nuevos productos saludables, seguros y listos para su uso. Por ello, los purés a base de hortalizas surgen como productos interesantes en la industria alimentaria. La calidad sensorial y nutricional de estos productos debe ser máxima para tener una buena aceptación por el consumidor, preservando en todo momento su seguridad alimentaria. Por ello, es necesario el procesado mediante técnicas menos agresivas, y ecoinnovadoras, que preserven la calidad de la materia prima durante una adecuada vida útil, frente a un tratamiento térmico convencional. El objetivo será estudiar el efecto de la alta presión hidrostática, una pasteurización por microondas de flujo continuo y otros métodos alternativos de calentamiento sobre el efecto en la calidad sensorial, nutritiva y microbiológica en diversos purés a base de hortalizas durante su vida comercial. [ENG] Frequently consumers demand new healthy, safe and ready to use products. Therefore, based purees vegetables arise as interesting products in the food industry. Sensory and nutritional quality of these products must be paramount to have good consumer acceptance, preserving food security. Therefore, processing by less aggressive techniques, and eco-innovative, which preserve the quality of the raw material for an adequate shelf life is necessary in relation a conventional heat treatment. The aim will be to study the effect of high hydrostatic pressure, flow microwave system pasteurization and other alternative heating methods on the effect on sensory, nutritional and microbiological quality in puree-based vegetables beverages during shelf life. Se agradece la financiación recibida por parte del Ministerio de Economía y Competitividad (MINECO), Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad AGL2013-48830-C2-1-R, a SAKATA SEEDS IBÉRICA, al Proyecto EUROLEGUME (EU FP7 Research Project No. 613781) de UE dentro del 7º Programa Marco de Investigación, Desarrollo Tecnológico y Diseminación y a los fondos FEDER. Se agradece especialmente la ayuda de la CNPq de Brasil por la beca de investigación concedida a Tâmmila V. Klug.

Background. Some algae are an excellent sources of vitamin B12, of special interest for vegetarian/vegan consumers, and of fucose to supplement fruit and vegetable beverages such as smoothies. Nevertheless, supplementation of smoothies with algae may lead to possible quality changes during smoothie shelf life that need to be studied. Therefore, the quality changes in fresh green smoothies supplemented (2.2%) with nine edible algae (sea lettuce, kombu, wakame, thongweed, dulse, Irish moss, nori, Spirulina and Chlorella) were studied throughout 24 days at 5 °C. Results. The initial vitamin C content – 238.7–326.0 mg kg−1 fresh weight (FW) – of a 200 g portion of any of the smoothies ensured full coverage of its recommended daily intake, and still supplying 50–60% of the recommended intake after 7 days. Chlorella and Spirulina smoothies showed the highest vitamin B12 content (33.3 and 15.3 µg kg−1 FW, respectively), while brown algae showed fucose content of 141.1–571.3 mg kg−1 FW. These vitamin B12 and fucose contents were highly maintained during shelf life. Conclusion. The Spirulina supplementation of a 200 g smoothie portion ensured full coverage of the recommended vitamin B12 intake, with lower vitamin C degradation, during a shelf life of 17 days. Furthermore, thongweed and kombu are also considered as excellent fucose sources with similar shelf life. The authors are grateful to Spanish Ministry of Economy and Competitiveness, Project AGL2013−48830−C2−1−R and FEDER for financial support. We would also like to thank SAKATA SEED IBERICA S.L. for providing Bimi® samples.

Thermal processing extends the shelf life of fruit and vegetables products by inactivating microorganisms and enzymes. The effect of a pasteurization (P) treatment, 90 °C ± 2 ºC for 35 s, provided by continuous semi-industrial microwave (MW) under different con-ditions (high power/short time and low power/long time) or conventional pasteurization (CP) on orange-colored smoothies and their changes throughout 45 days of storage at 5 °C were investigated. Results indicated that vitamin C and antioxidant capacity (FRAP) in CP decreased dramatically in comparison with the unheated and MWP smoothies. On the contrary, all heating treatments increased the contents of total phenolic compounds (TPC) and carotenoids. Based on the sensory quality and microbial counts, the shelf life of all those heated smoothies reached 45 days. No Listeria monocytogenes growth was found and all microbial counts were below the European legal limits. MWP as compared to the CP method led to a greater reduction of mesophilic bacteria after 45 days at 5 °C (3.7 log cfu g-1 for CP and 1.6 log cfu g-1 for MWP). When highest power and shortest time MWP treatments were used (3,600 W for 93 s), FRAP and vitamin C were better preserved. This work was financially supported by MINECO-FEDER (AGL2013-48830-C2-1-R). Thanks are due to Instituto de Biotecnología Vegetal (IBV-UPCT) for providing some of the equipment

A green fresh vegetables smoothie rich in health-promoting compounds was developed. Two thermal treatments to reduce microbial load and preserve quality were assayed: T1 (3 min at 80ºC) and T2 (45 s at 90ºC). Fresh blended unheated samples were used as control (CRL). The smoothie presented a viscoelastic behaviour. Thermal treatments reduced initial microbial loads by 1.3-2.4 and 1.4-3.1 log units, respectively. Samples were stored in darkness at 5 and 15ºC. Colour and physicochemical changes were reduced in thermal-treated samples throughout storage, which were better preserved at 5ºC rather than at 15ºC. Vitamin C changes during storage were fitted with a Weibullian distribution. Total vitamin C losses of T1 and T2 samples during storage at 15ºC were greatly reduced when they were stored at 5ºC. Initial total phenolics content (151.1 mg 29 kg-1 fw) was 44 and 36% increased after T1 and T2 treatments, respectively. The 3-p-30 coumaroyl quinic and chlorogenic acids accounted the 84.7% and 7.1% relative 31 abundance, respectively. Total antioxidant capacity (234.2 mg Trolox equivalent kg-1 fw) remained constant after the thermal treatments and was better maintained during storage in thermal-treated samples. Glucobrassicin accounted the 81% of the initial total glucosinolates content (117.8 mg kg-1 fw) of the smoothie. No glucosinolates losses were observed after T2 treatment being better preserved in thermal-treated samples. Conclusively, a short time–high temperature mild thermal treatment (T2) showed better quality and bioactive compounds retention in a green fresh vegetable smoothie during low temperature storage. The authors are grateful to Spanish Ministry of Economy and Competitiveness (MINECO) Project AGL2013-48830-C2-1-R and FEDER for financial support. We are grateful to M. Otón for his skilful technical assistance.