1. Simultaneous detection of beta-galactosidase activity and surface antigen expression in viable haematopoietic cells.
- Author
-
Berger CN, Tan SS, and Sturm KS
- Subjects
- Animals, Antigens, Surface blood, Biomarkers, Crosses, Genetic, Female, Flow Cytometry, Fluoresceins, Galactosides, Liver embryology, Liver enzymology, Male, Mice, Mice, Transgenic, Thymus Gland embryology, Thymus Gland enzymology, beta-Galactosidase biosynthesis, Antigens, Surface analysis, Hematopoietic Stem Cells enzymology, Hematopoietic Stem Cells immunology, beta-Galactosidase metabolism
- Abstract
The quantitation of intracellular beta-galactosidase activity has been described for viable cells. By using the fluorogenic substrate fluorescein-di-beta-D-galactopyranoside (FDG) in conjunction with flow cytometry, the proportion of positive cells as well as the level of expression can be determined. In this paper we describe beta-galactosidase expression in lymphoid and myeloid cells from transgenic mice that widely express beta-galactosidase from an inserted lacZ transgene. Both foetal and adult haematopoietic tissues are able to express beta-galactosidase. The intracellular fluorescence reflecting beta-galactosidase activity can be readily combined with fluorescently labelled antibodies against cell surface antigens. Thus, beta-galactosidase can be used as a marker in transplantation experiments to study the development of lymphoid and myeloid precursor cells.
- Published
- 1994
- Full Text
- View/download PDF