1. Numb confers to inhibit epithelial mesenchymal transition via β-catenin/Lin28 signaling pathway in breast cancer.
- Author
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Hu XB, Ouyang LZ, He Y, and Xia MZ
- Subjects
- Breast Neoplasms genetics, Breast Neoplasms pathology, Cadherins genetics, Cadherins metabolism, Cell Line, Tumor, Cell Movement genetics, Cell Proliferation genetics, Epithelial-Mesenchymal Transition genetics, Female, Humans, MCF-7 Cells, Membrane Proteins genetics, Nerve Tissue Proteins genetics, RNA Interference, RNA-Binding Proteins genetics, Signal Transduction genetics, beta Catenin genetics, Breast Neoplasms metabolism, Membrane Proteins metabolism, Nerve Tissue Proteins metabolism, RNA-Binding Proteins metabolism, beta Catenin metabolism
- Abstract
Objectives: This study aimed to investigate role of Numb in the epithelial mesenchymal transition (EMT) of breast cancer., Methods: Numb and β-catenin were inhibited in MCF-7 cells using sh-RNA and overexpressed in T47D cells by pcDNA3.0-Numb, pcDNA3.0-β-catenin. Cell proliferation, invasion and migration were evaluated using MTT and Transwell assay, respectively. β-catenin, Lin28, and EMT related markers were determined using qRT-PCR and Western Blotting., Results: Knockdown of Numb significantly promoted the proliferation, invasion and migration of MCF-7 cells, further increased the expression of β-catenin, Lin28, Snail-1, and N-cadherin, as well as decreased E-cadherin. In T47D cells transfected with pcDNA3.0-Numb, the results were quite the reverse., Conclusions: Knockdown of Numb could promote the EMT of breast cancer cells via β-cateni/Lin28 signaling pathway., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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