1. Bet v 1 from birch pollen is a hypoallergen with vitamin D3 in the pocket.
- Author
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Hufnagl, Karin, Kromp, Livia, Bianchini, Rodolfo, Afify, Sheriene Moussa, Wiederstein, Markus, Redegeld, Frank A., Zuvalova, Iveta, Dvorak, Zdenek, Hofstetter, Gerlinde, Roth‐Walter, Franziska, Pacios, Luis F., and Jensen‐Jarolim, Erika
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CHOLECALCIFEROL ,MONONUCLEAR leukocytes ,IMMUNOGLOBULIN E ,MILK allergy ,TRETINOIN ,POLLEN ,REGULATORY T cells ,BIRCH - Abstract
Based on our I in silico i structural analyses, the IgE-specific effects may be due to epitope masking by the VD3 ligand of two previously described IgE-binding B-cell epitope regions in the molecular surface of Bet v 1 (Figure 1C). Keywords: Bet v 1; birch pollen allergy; immunomodulation; lipocalin-like; vitamin D EN Bet v 1 birch pollen allergy immunomodulation lipocalin-like vitamin D 3801 3804 4 12/01/21 20211201 NES 211201 Abbreviations ANS 1-anilino-8-naphthalene sulfonate BCA bicinchoninic acid BP birch pollen E aff affinity energy FCS foetal calf serum IL interleukin K D binding constant kDa kilodalton MFI mean fluorescence intensity OD optical density PBMCs peripheral blood mononuclear cells PDB protein data bank RA retinoic acid RFUs relative fluorescence units VD3 Vitamin D3 VDRE vitamin D response element ACKNOWLEDGEMENTS We thank Bart R. Blokhuis for excellent technical assistance. [Extracted from the article]
- Published
- 2021
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