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Bay leaves (Syzygium polyanthum) and a second wood (Caesalpinia sappan L) are widely known to the public as natural antiseptics for prevention and treatment, including treating digesting diseases, one of which is diarrhea caused by Escherichia coli. Bay leaves and Secang wood can be used as antiseptics because they contain the active substance tannin. This research aims to determine the difference in the antibacterial effectiveness of 96% ethanol extract of bay leaves (Syzygium polyanthum) and 96% ethanol extract of second wood (Caesalpinia sappan L) on growth Escherichia coli bacteria. The type of research is experimental. The method used in this research is the Kirby-Bauer diffusion method. This method observes the clear zone produced from a blank disk that has been treated with bay leaf extract and second wood extract at a concentration of 30%, 60%, and 90% for incubation at 37⁰C for 24 hours. From the research results, it can be concluded that the ethanol extract of bay leaves with a concentration of 30%, 60%, and 90% with an inhibition zone diameter of 0 mm, 7,6 mm, and 9,6 mm. The second wood at a concentration of 30%, 60%, and 90% with an inhibition zone diameter of 10 mm, 11,3 mm, and 14 mm. The statistical test shows that the data is normally distributed with a parametric test ANOVA, p =0,00, which means that there is no significant difference in the diameter of the inhibition zone between the ethanol extract of bay leaves and the ethanol extract of second wood. [ABSTRACT FROM AUTHOR]