1. Increased CD123 + HLA-DR - Granulocytes in Allergic Rhinitis and Influence of Allergens on Expression of Cell Membrane Markers.
- Author
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Xie H, Zhang H, Chen D, Cheng L, Gu F, Wang S, Liu M, Li L, Zeng Q, and He S
- Subjects
- Humans, Male, Female, Adult, Middle Aged, Flow Cytometry, Animals, Cell Membrane metabolism, Antigens, Dermatophagoides immunology, Pyroglyphidae immunology, Young Adult, Granulocytes immunology, Granulocytes metabolism, HLA-DR Antigens metabolism, HLA-DR Antigens immunology, Allergens immunology, Rhinitis, Allergic immunology, Rhinitis, Allergic diagnosis, Basophils immunology, Basophils metabolism, Interleukin-3 Receptor alpha Subunit metabolism, Interleukin-3 Receptor alpha Subunit immunology, Biomarkers metabolism
- Abstract
Background: It is reported that CD123 + HLA-DR- cells in PBMC are basophils, and CD203c, CD63, and FcεRI molecules are activation markers of basophils. However, little is known of CD123 + HLA-DR-cells in blood granulocytes., Objective: To investigate the presence of CD123 + HLA-DR- cells in the blood granulocytes and peripheral PBMC of patients with allergic rhinitis (AR), as well as the impact of allergens on the cell membrane markers of basophils., Methods: Flow cytometry was used to detect the expression of the membrane molecules., Results: While CD123 + HLA-DR- PBMCs are representative of basophils, their presence did not significantly change in patients with AR. In contrast, both the percentage and number of CD123 + HLA-DR- granulocytes, which make up only up to 50% of basophils, were significantly increased in patients with seasonal (sAR) and perennial AR (pAR). CD63+, CD203c+, and FcεRIα+ cells within CD123 + HLA-DR- granulocytes also showed enhanced activity in patients with AR. Allergen extracts from house dust mite allergen extract (HDME) and Artemisia sieversiana wild extract further increased the number of CD123 + HLA-DR- cells in granulocytes of sAR and pAR patients, as well as in PBMCs of pAR patients., Conclusions: The use of CD123 + HLA-DR- granulocytes and PBMC may not be sufficient for diagnosing AR. Allergens could potentially contribute to the development of AR by influencing the number of CD123 + HLA-DR- cells, as well as the expression of CD63, CD203c, and FcεRIαin these cells.
- Published
- 2024
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