1. Exploring multisite heterogeneity of human basal cell carcinoma proteome and transcriptome.
- Author
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Berl, Ariel, Shir-az, Ofir, Genish, Ilai, Biran, Hadas, Mann, Din, Singh, Amrita, Wise, Julia, Kravtsov, Vladimir, Kidron, Debora, Golberg, Alexander, Vitkin, Edward, Yakhini, Zohar, and Shalom, Avshalom
- Subjects
BASAL cell carcinoma ,GENE expression ,GENE expression profiling ,HETEROGENEITY ,TRANSCRIPTOMES ,PROTEOMICS - Abstract
Basal cell carcinoma (BCC) is the most common type of skin cancer. Due to multiple, potential underlying molecular tumor aberrations, clinical treatment protocols are not well-defined. This study presents multisite molecular heterogeneity profiles of human BCC based on RNA and proteome profiling. Three areas from lesions excised from 9 patients were analyzed. The focus was gene expression profiles based on proteome and RNA measurements of intra-tumor heterogeneity from the same patient and inter-tumor heterogeneity in nodular, infiltrative, and superficial BCC tumor subtypes from different patients. We observed significant overlap in intra- and inter-tumor variability of proteome and RNA expression profiles, showing significant multisite heterogeneity of protein expression in the BCC tumors. Inter-subtype analysis has also identified unique proteins for each BCC subtype. This profiling leads to a deeper understanding of BCC molecular heterogeneity and potentially contributes to developing new sampling tools for personalized diagnostics therapeutic approaches to BCC. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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