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Start Over Author "Labarde A" Topic bacteriophages
4 results on '"Labarde A"'

1. A non-invasive method for studying viral DNA delivery to bacteria reveals key requirements for phage SPP1 DNA entry in Bacillus subtilis cells.

Author

Fernandes S, Labarde A, Baptista C, Jakutytè L, Tavares P, and São-José C

Subjects
Bacillus subtilis metabolism, Calcium metabolism, Genome, Viral, Gramicidin pharmacology, Membrane Potentials, Microscopy, Fluorescence, Molecular Imaging, Virus Internalization drug effects, Virus Uncoating, Bacillus subtilis virology, Bacteriophages physiology, DNA, Viral, Gene Transfer Techniques, Transduction, Genetic
Abstract

Bacteriophages use most frequently a tail apparatus to create a channel across the entire bacterial cell envelope to transfer the viral genome to the host cell cytoplasm, initiating infection. Characterization of this critical step remains a major challenge due to the difficulty to monitor DNA entry in the bacterium and its requirements. In this work we developed a new method to study phage DNA entry that has the potential to be extended to many tailed phages. Its application to study genome delivery of bacteriophage SPP1 into Bacillus subtilis disclosed a key role of the host cell membrane potential in the DNA entry process. An energized B. subtilis membrane and a millimolar concentration of calcium ions are shown to be major requirements for SPP1 DNA entry following the irreversible binding of phage particles to the receptor YueB., (Copyright © 2016 Elsevier Inc. All rights reserved.)

Published
2016
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2. Temporal compartmentalization of viral infection in bacterial cells

Author

Paulo Tavares, Lina Jakutyte, Thorsten Mielke, Silvia Ayora, Cyrille Billaudeau, Rut Carballido-López, Audrey Labarde, Beatrix Fauler, Prishila Ponien, María López-Sanz, Eric Jacquet, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), AgroParisTech, Institut de Chimie des Substances Naturelles (ICSN), and Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)

Subjects
DNA Replication, Time Factors, viruses, DNA-Directed DNA Polymerase, Biology, Genome, Virus, Bacteriophage, 03 medical and health sciences, chemistry.chemical_compound, Capsid, Multienzyme Complexes, Compartment (development), Bacteriophages, 030304 developmental biology, bacteriophage, virus infection, phage DNA replication, virus assembly, bacterial cell compartmentalization, 0303 health sciences, Multidisciplinary, 030302 biochemistry & molecular biology, Virion, Biological Sciences, biology.organism_classification, Cell Compartmentation, Cell biology, Nucleoprotein, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, chemistry, Virus Diseases, Cytoplasm, DNA, Viral, Host-Pathogen Interactions, Replisome, DNA, Bacillus subtilis
Abstract

International audience; Virus infection causes major rearrangements in the subcellular architecture of eukaryotes, but its impact in prokaryotic cells was much less characterized. Here, we show that infection of the bacterium Bacillus subtilis by bacteriophage SPP1 leads to a hijacking of host replication proteins to assemble hybrid viral–bacterial replisomes for SPP1 genome replication. Their biosynthetic activity doubles the cell total DNA content within 15 min. Replisomes operate at several independent locations within a single viral DNA focus positioned asymmetrically in the cell. This large nucleoprotein complex is a self-contained compartment whose boundaries are delimited neither by a membrane nor by a protein cage. Later during infection, SPP1 procapsids localize at the periphery of the viral DNA compartment for genome packaging. The resulting DNA-filled capsids do not remain associated to the DNA transactions compartment. They bind to phage tails to build infectious particles that are stored in warehouse compartments spatially independent from the viral DNA. Free SPP1 structural proteins are recruited to the dynamic phage-induced compartments following an order that recapitulates the viral particle assembly pathway. These findings show that bacteriophages restructure the crowded host cytoplasm to confine at different cellular locations the sequential processes that are essential for their multiplication.

Published
2021

3. A non-invasive method for studying viral DNA delivery to bacteria reveals key requirements for phage SPP1 DNA entry in Bacillus subtilis cells

Author

Catarina Baptista, Audrey Labarde, Lina Jakutyte, Carlos São-José, Sofia Fernandes, Paulo Tavares, iMed.ULisboa - Research Institute for Medicine, Universidade de Libsoa-Faculty of Pharmacy, Laboratoire de Virologie Moléculaire et Structurale, Centre National de la Recherche Scientifique ( CNRS ), Bactériophages des bactéries gram positive ( PHAG+ ), Département Virologie ( Dpt Viro ), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ), Instituto de Medicina Molecular (iMM), Faculdade de Medicina [Lisboa], Universidade de Lisboa = University of Lisbon (ULISBOA)-Universidade de Lisboa = University of Lisbon (ULISBOA), Centre National de la Recherche Scientifique (CNRS), Bactériophages des bactéries gram positive (PHAG+ ), Département Virologie (Dpt Viro), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), and Universidade de Lisboa (ULISBOA)-Universidade de Lisboa (ULISBOA)

Subjects
0301 basic medicine, Phagemid, [SDV]Life Sciences [q-bio], Bacillus subtilis, Genome, Viral, Biology, Bacterial cell structure, Microbiology, Membrane Potentials, Bacteriophage, 03 medical and health sciences, chemistry.chemical_compound, Viral entry, Transduction, Genetic, Virology, Virus Uncoating, Bacteriophages, Host cell membrane, [ SDV ] Life Sciences [q-bio], Gene Transfer Techniques, Gramicidin, Virus Internalization, biology.organism_classification, Cell biology, Molecular Imaging, 030104 developmental biology, chemistry, Microscopy, Fluorescence, Host cell cytoplasm, DNA, Viral, Calcium, DNA
Abstract

International audience; Bacteriophages use most frequently a tail apparatus to create a channel across the entire bacterial cell envelope to transfer the viral genome to the host cell cytoplasm, initiating infection. Characterization of this critical step remains a major challenge due to the difficulty to monitor DNA entry in the bacterium and its requirements. In this work we developed a new method to study phage DNA entry that has the potential to be extended to many tailed phages. Its application to study genome delivery of bacteriophage SPP1 into Bacillus subtilis disclosed a key role of the host cell membrane potential in the DNA entry process. An energized B. subtilis membrane and a millimolar concentration of calcium ions are shown to be major requirements for SPP1 DNA entry following the irreversible binding of phage particles to the receptor YueB.

Published
2016

4. The Revisited Genome of Bacillus subtilis Bacteriophage SPP1.

Author

Godinho, Lia M., El Sadek Fadel, Mehdi, Monniot, Céline, Jakutyte, Lina, Auzat, Isabelle, Labarde, Audrey, Djacem, Karima, Oliveira, Leonor, Carballido-Lopez, Rut, Ayora, Silvia, and Tavares, Paulo

Subjects
BACTERIAL genomes, BACILLUS subtilis, BACTERIOPHAGES, NUCLEOTIDE sequence, DNA replication
Abstract

Bacillus subtilis bacteriophage SPP1 is a lytic siphovirus first described 50 years ago. Its complete DNA sequence was reported in 1997. Here we present an updated annotation of the 44,016 bp SPP1 genome and its correlation to different steps of the viral multiplication process. Five early polycistronic transcriptional units encode phage DNA replication proteins and lysis functions together with less characterized, mostly non-essential, functions. Late transcription drives synthesis of proteins necessary for SPP1 viral particles assembly and for cell lysis, together with a short set of proteins of unknown function. The extensive genetic, biochemical and structural biology studies on the molecular mechanisms of SPP1 DNA replication and phage particle assembly rendered it a model system for tailed phages research. We propose SPP1 as the reference species for a new SPP1-like viruses genus of the Siphoviridae family. [ABSTRACT FROM AUTHOR]

Published
2018
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