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1. Enhancing the Thermal Stability and Enzyme Activity of Ketopantoate Hydroxymethyltransferase through Interface Modification Engineering.

2. Development of a new chemo-enzymatic catalytic route for synthesis of (S)- 2-chlorophenylglycine.

3. Functional expression of an echinocandin B deacylase from Actinoplanes utahensis in Escherichia coli.

4. Improvement of pyrroloquinoline quinone-dependent d-sorbitol dehydrogenase activity from Gluconobacter oxydans via expression of Vitreoscilla hemoglobin and regulation of dissolved oxygen tension for the biosynthesis of 6-(N-hydroxyethyl)-amino-6-deoxy-α-l-sorbofuranose.

5. Characterization of a recombinant sucrose isomerase and its application to enzymatic production of isomaltulose.

6. Highly Efficient Chemoenzymatic Synthesis of l-Phosphinothricin from N -Phenylacetyl-d,l-phosphinothricin by a Robust Immobilized Amidase.

7. Covalent immobilization of recombinant Citrobacter koseri transaminase onto epoxy resins for consecutive asymmetric synthesis of L-phosphinothricin.

8. Purification and Biochemical Characterization of a Tyrosine Phenol-lyase from Morganella morganii.

9. Efficient synthesis of L-phosphinothricin using a novel aminoacylase mined from Stenotrophomonas maltophilia.

10. Construction of a highly active secretory expression system in Bacillus subtilis of a recombinant amidase by promoter and signal peptide engineering.

11. Semi-rational engineering of a Kluyveromyces lactis aldo-keto reductase KlAKR for improved catalytic efficiency towards t-butyl 6-cyano-(3R, 5R)-dihydroxyhexanoate.

12. Metabolic engineering of Escherichia coli for d-pantothenic acid production.

13. Synergistic improvement of PQQ-dependent D-sorbitol dehydrogenase activity from Gluconobacter oxydans for the biosynthesis of miglitol precursor 6-(N-hydroxyethyl)-amino-6-deoxy-α-L-sorbofuranose.

14. Efficient biosynthesis of (R)-3-amino-1-butanol by a novel (R)-selective transaminase from Actinobacteria sp.

15. Highly efficient conversion of 1-cyanocycloalkaneacetonitrile using a "super nitrilase mutant".

16. Identification and engineering of the key residues at the crevice-like binding site of lipases responsible for activity and substrate specificity.

17. Statistical medium optimization and DO-STAT fed-batch fermentation for enhanced production of tyrosine phenol lyase in recombinant Escherichia coli.

18. Significant improvement of the nitrilase activity by semi-rational protein engineering and its application in the production of iminodiacetic acid.

19. Process development for efficient biosynthesis of L-DOPA with recombinant Escherichia coli harboring tyrosine phenol lyase from Fusobacterium nucleatum.

20. Biochemical characterization of a novel tyrosine phenol-lyase from Fusobacterium nucleatum for highly efficient biosynthesis of l-DOPA.

21. Production of R-Mandelic Acid Using Nitrilase from Recombinant E. coli Cells Immobilized with Tris(Hydroxymethyl)Phosphine.

22. Biocatalytic hydrolysis of chlorinated nicotinamides by a superior AS family amidase and its application in enzymatic production of 2-chloronicotinic acid.

23. Biocatalytic production of (S)-2-aminobutanamide by a novel d-aminopeptidase from Brucella sp. with high activity and enantioselectivity.

24. Semi-Rational Engineering of Leucine Dehydrogenase for L-2-Aminobutyric Acid Production.

25. Properties of a novel thermostable glucose isomerase mined from Thermus oshimai and its application to preparation of high fructose corn syrup.

26. Identification and characterization of a novel amidase signature family amidase from Parvibaculum lavamentivorans ZJB14001.

27. Application of CRISPRi in Corynebacterium glutamicum for shikimic acid production.

28. Mining and characterization of two amidase signature family amidases from Brevibacterium epidermidis ZJB-07021 by an efficient genome mining approach.

29. Exploitation and characterization of three versatile amidase super family members from Delftia tsuruhatensis ZJB-05174.

30. Efficient production of methionine from 2-amino-4-methylthiobutanenitrile by recombinant Escherichia coli harboring nitrilase.

31. Purification, gene cloning, and characterization of a novel halohydrin dehalogenase from Agromyces mediolanus ZJB120203.

32. Improvement of Alcaligenes faecalis nitrilase by gene site saturation mutagenesis and its application in stereospecific biosynthesis of (R)-(-)-mandelic acid.

33. Screening and Improving the Recombinant Nitrilases and Application in Biotransformation of Iminodiacetonitrile to Iminodiacetic Acid.

34. Isolation of enantioselective α-hydroxyacid dehydrogenases based on a high-throughput screening method.

35. Enzymatic production of 5'-inosinic acid by a newly synthesised acid phosphatase/phosphotransferase.

36. Screening, cultivation, and biocatalytic performance of Rhodococcus boritolerans FW815 with strong 2,2-dimethylcyclopropanecarbonitrile hydratase activity.

37. Gene cloning, expression, and characterization of a nitrilase from Alcaligenes faecalis ZJUTB10.

38. Characterization of a newly isolated strain Rhodococcus erythropolis ZJB-09149 transforming 2-chloro-3-cyanopyridine to 2-chloronicotinic acid.

39. Purification and characterization of 3-ketovalidoxylamine A C-N lyase produced by Stenotrophomonas maltrophilia.

40. Isolation and characterization of Delftia tsuruhatensis ZJB-05174, capable of R-enantioselective degradation of 2,2-dimethylcyclopropanecarboxamide.

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