1. Dissemination of Carbapenem-resistant Klebsiella pneumoniae clinical isolates with various combinations of Carbapenemases (KPC-2, NDM-1, NDM-4, and OXA-48) and 16S rRNA Methylases (RmtB and RmtC) in Vietnam.
- Author
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Tada T, Tsuchiya M, Shimada K, Nga TTT, Thu LTA, Phu TT, Ohmagari N, and Kirikae T
- Subjects
- Anti-Bacterial Agents pharmacology, Bacterial Proteins metabolism, Humans, Klebsiella pneumoniae isolation & purification, Methyltransferases genetics, Microbial Sensitivity Tests, Multilocus Sequence Typing, Phylogeny, Vietnam, beta-Lactam Resistance drug effects, Bacterial Proteins genetics, Carbapenems pharmacology, Klebsiella pneumoniae drug effects, Klebsiella pneumoniae genetics, beta-Lactam Resistance genetics, beta-Lactamases genetics
- Abstract
Methods: Twenty-seven clinical isolates of carbapenem-resistant Klebsiella pneumoniae with MICs ≥4 mg/L for imipenem or meropenem were obtained from inpatients in a hospital in Vietnam. Antimicrobial susceptibility tests and whole genome sequencing were performed. Multilocus sequence typing and the presence of drug resistant genes were determined and a maximum-likelihood phylogenetic tree was constructed by SNP alignment of whole genome sequencing data., Results: All the isolates harbored one of genes encoding carbapenemases, including KPC-2, NDM-1, NDM-4 and OXA-48. Of the isolates, 13 were resistant to arbekacin with MICs ≥256 mg/L and to amikacin with MICs ≥512 mg/L. These isolates harbored a gene encoding a 16S rRNA methylase, either RmtB or RmtC. Eighteen and 4 isolates belonged to international clones, ST15 and ST16, respectively. None of the isolates had colistin-resistant factors., Conclusion: Carbapenem-resistant K. pneumoniae isolates belonged to international clones spread in a medical setting in Vietnam, and that these isolates harbored genes encoding various combinations of carbapenemases and 16S rRNA methylases. This is the first report of KPC-2, NDM-4 and OXA-48 producers in a medical setting in Vietnam.
- Published
- 2017
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