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Start Over Author "Ag. Leroy" Topic bacterial proteins
2 results on '"Ag. Leroy"'

1. Antibiotic resistance heterogeneity and LasR diversity within Pseudomonas aeruginosa populations from pneumonia in intensive care unit patients.

Author

Crémet L, Leroy AG, Muller D, Delanou S, Burghelea A, Broquet A, Roquilly A, and Caroff N

Subjects
Bacterial Proteins metabolism, Bronchoalveolar Lavage, Ceftazidime pharmacology, DNA, Bacterial, Genetic Variation, Humans, Imipenem pharmacology, Intensive Care Units, Microbial Sensitivity Tests, Mutation, Quorum Sensing, Trans-Activators metabolism, beta-Lactams pharmacology, Anti-Bacterial Agents pharmacology, Bacterial Proteins genetics, Drug Resistance, Bacterial, Pneumonia microbiology, Pseudomonas Infections microbiology, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa genetics, Trans-Activators genetics
Abstract

This study investigated within-host heterogeneity of 66 Pseudomonas aeruginosa populations from pneumonia in 51 critically ill ventilated patients by examining 30 colonies per bronchoalveolar lavage (BAL). Differences in antibiotic susceptibility and quorum-sensing (QS) phenotypes were observed between the members of 14 (21.2%) and 10 (15.2%) populations, respectively. A significant association was found between QS deficiency and ceftazidime resistance. QS deficiency was associated with various lasR modifications, and was observed in 25 of 51 (49.0%) patients, including seven patients who received ≤48 h of ventilation. This study confirms the need to examine diverse colonies when analysing BAL cultures, particularly in β-lactam-exposed patients, to avoid missing ceftazidime- or imipenem-resistant isolates., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Published
2021
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2. Catheter-related bloodstream infection due to Tsukamurella pulmonis identified by MALDI-TOF spectrometry, 16S rRNA gene sequencing, and secA1 gene sequencing in an immunocompromised child: a case report and literature review

Author

Pascale Bémer, Ag. Leroy, Aurélie Guillouzouic, M. E. Juvin, F. Chantreau, Elise Persyn, L. Ruffier d'Epenoux, Elise Launay, Eve-Marie Takoudju, J. El Khobzi, and Stéphane Corvec

Subjects
Microbiology (medical), Tsukamurella, DNA, Bacterial, Bacilli, Bacteremia, medicine.disease_cause, DNA sequencing, Microbiology, Immunocompromised Host, Bacterial Proteins, Bloodstream infection, RNA, Ribosomal, 16S, medicine, Central Venous Catheters, Humans, Tsukamurella pulmonis, biology, Infant, General Medicine, Sequence Analysis, DNA, biology.organism_classification, 16S ribosomal RNA, medicine.disease, Anti-Bacterial Agents, Actinobacteria, Infectious Diseases, Treatment Outcome, Catheter-Related Infections, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Female, Actinomyces
Abstract

Tsukamurella species are Gram-positive bacilli related to aerobic Actinomyces. Originally reported from the environment, Tsukamurella species have also been described in human infections, especially in bacteremia. A literature review analysis revealed that Tsukamurella spp. are often initially considered as contaminant microorganisms, especially due to bacterial identification issues. Here, we report a catheter-related bloodstream infection in an immunocompromised child caused by Tsukamurella pulmonis. Matrix-Assisted Laser Desorption/Ionization-Time Of Flight (MALDI-TOF) mass spectrometry allowed rapid genus-level identification and contributed to better patient care. However, accurate species-level identification required 16S rRNA gene sequencing and secA1 gene sequencing. Considering the increased number of Tsukamurella infections, the implementation of new Tsukamurella species in MALDI-TOF databases is required to be more discriminant.

Published
2020

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