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The richness and diversity of bacterial community in the fermented pit mud from the new and old distilleries of Shedian Liquor were analysed via the Illumina Miseq PE300 high-throughput sequencing technology, so as to further understand the differences of bacterial community structure in fermented pit muds with different pit ages. The results show that the bacterial community is mainly distributed into five dominant phyla (> 1.0%): Firmicutes, Synergistes, Bacteroidetes, Proteobacteria and Verrucomicrobia. There are 13 dominant genera (> 1.0%), of which Lactobacillus is dominant in both samples with a higher content in the new pit (61.12%) and lower in the old pit (1.15%). Caproiciproducens accounting for 43.53% and Syntrophomonas accounting for 6.20%, norank_f__Family_XIV (17.68%), Aminobacterium (17.68%), Sedimentibacter (1.89%), Caldicoprobacter (1.52%) and Sporanaerobacter (1.29%) are the dominant bacteria in the old pit mud. Bacillus accounting for 6.02%, Bacteroides (6.27%), Akkermansia (1.51%), Hydrogenispora (2.15%) and norank_f__Bacteroidales_S24-7_group (1.15%) are the dominant bacteria in the new pit mud. The results showed that there is a great difference in the bacterial community structure of in the new and old pit mud. In addition, compared with the bacterial community composition in the pit mud of Luzhou flavour Baijiu in Sichuan, it was found that the microorganisms that formed caproic acid and butyric acid were different. [ABSTRACT FROM AUTHOR]

The physicochemical indexes of bag Daqu and brick Daqu were studied. With high-throughput sequencing technology the bacterial community structure of bag Daqu and brick Daqu samples were analyzed. The results showed that the moisture content, acidity, starch content and other physicochemical indexes of brick Daqu and bag Daqu were within the standard range of finished Daqu. Compared with bag Daqu, the moisture content and fermentation power of brick Daqu were higher and the difference was significant; the saccharification power, liquefaction power, esterification power and fermentation power brick Daqu were higher, the acidity and starch content of brick Daqu were lower and the difference was very significant; the starch content was not much different. With the average relative abundance of 0. 05% as the threshold, the dominant bacteria of the bag Daqu and brick Daqu were Proteobacteria, Firmicutes and Actinobacteria. There was one more Bacteroidetes in the dominant bacteria of he brick Daqu than in bag Daqu. With the average relative abundance of 1. 00% as the threshold, the predominant bacterial genus of bag Daqu were Pseudomonas, Pantoea, Acinetobacter, Leuconostoc, Lactobacillus, and the predominant bacterial genus of brick Daqu were Lactobacillus, Leuconostoc, Pantoea, Klebsiella, Pseudomonas, Staphylococcus, Lactococcus. In this study, Barnesiella was discovered from the brick Daqu for the first time. [ABSTRACT FROM AUTHOR]