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Your search keyword '"Joris P. Jansen"' showing total 2 results
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2 results on '"Joris P. Jansen"'

1. Host–Receptor Post-Translational Modifications Refine Staphylococcal Leukocidin Cytotoxicity

Author

Jos A. G. van Strijp, Michael T. McManus, Carla J. C. de Haas, Michael Boettcher, Michiel van Gent, András N Spaan, Lisette M Scheepmaker, Anneroos Velthuizen, Bart W. Bardoel, Joris P Jansen, Robert Jan Lebbink, Angelino T. Tromp, Kok P. M. van Kessel, and Pieter-Jan A. Haas

Subjects
Staphylococcus aureus, Cell Survival, Health, Toxicology and Mutagenesis, Cell Culture Techniques, Leukocidin, lcsh:Medicine, receptors, Computational biology, Biology, Toxicology, Article, leukocidins, Receptors, G-Protein-Coupled, 03 medical and health sciences, Drug Resistance, Bacterial, post-translational modifications, Humans, CRISPR, Genetic Predisposition to Disease, Cytotoxicity, Receptor, skin and connective tissue diseases, Gene, 030304 developmental biology, G protein-coupled receptor, Phagocytes, 0303 health sciences, Host Microbial Interactions, 030306 microbiology, Cas9, lcsh:R, U937 Cells, Staphylococcal Infections, respiratory system, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, HEK293 Cells, G-protein coupled receptors, bi-component pore-forming toxins, bacteria, Ectopic expression, CRISPR-Cas Systems, Protein Processing, Post-Translational, Genome-Wide Association Study, Protein Binding
Abstract

Staphylococcal bi-component pore-forming toxins, also known as leukocidins, target and lyse human phagocytes in a receptor-dependent manner. S-components of the leukocidins Panton-Valentine leukocidin (PVL), &gamma, haemolysin AB (HlgAB) and CB (HlgCB), and leukocidin ED (LukED) specifically employ receptors that belong to the class of G-protein coupled receptors (GPCRs). Although these receptors share a common structural architecture, little is known about the conserved characteristics of the interaction between leukocidins and GPCRs. In this study, we investigated host cellular pathways contributing to susceptibility towards S. aureus leukocidin cytotoxicity. We performed a genome-wide CRISPR/Cas9 library screen for toxin-resistance in U937 cells sensitized to leukocidins by ectopic expression of different GPCRs. Our screen identifies post-translational modification (PTM) pathways involved in the sulfation and sialylation of the leukocidin-receptors. Subsequent validation experiments show differences in the impact of PTM moieties on leukocidin toxicity, highlighting an additional layer of refinement and divergence in the staphylococcal host-pathogen interface. Leukocidin receptors may serve as targets for anti-staphylococcal interventions and understanding toxin-receptor interactions will facilitate the development of innovative therapeutics. Variations in the genes encoding PTM pathways could provide insight into observed differences in susceptibility of humans to infections with S. aureus.

Published
2020
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2. Human CD45 is an F-component-specific receptor for the staphylococcal toxin Panton-Valentine leukocidin

Author

Thomas Henry, Jos A. G. van Strijp, Pauline Abrial, Robert Jan Lebbink, Emilie Bourdonnay, Bart W. Bardoel, Gerard Lina, Kok P. M. van Kessel, François Vandenesch, Michael T. McManus, Angelino T. Tromp, Michiel van Gent, Elisabeth Kruse, Carla J. C. de Haas, Joris P Jansen, András N Spaan, Amandine Martin, Christopher J. Day, Michael P. Jennings, Michael Boettcher, Pieter-Jan A. Haas, University Medical Center [Utrecht], University of Chicago, Inflammasome, Infections bactériennes et autoinflammation, Inflammasome, Bacterial Infections and Autoinflammation (I2BA), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of California [San Francisco] (UCSF), University of California, Griffith University [Brisbane], Pathogénie des Staphylocoques – Staphylococcal Pathogenesis (StaPath), Rockefeller University [New York], Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of California [San Francisco] (UC San Francisco), University of California (UC), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Microbiology (medical), Staphylococcus aureus, Neutrophils, 030106 microbiology, Immunology, Bacterial Toxins, Leukocidin, Exotoxins, Complement C5a, Biology, medicine.disease_cause, Applied Microbiology and Biotechnology, Microbiology, Cell Line, 03 medical and health sciences, Hemolysin Proteins, Mice, Bacterial Proteins, Leukocidins, Genetics, medicine, Animals, Humans, Receptor, skin and connective tissue diseases, Receptor, Anaphylatoxin C5a, Mice, Knockout, Toxin, Cell Biology, respiratory system, biochemical phenomena, metabolism, and nutrition, Staphylococcal Infections, bacterial infections and mycoses, Phenotype, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Bacterial Load, Disease Models, Animal, Cell culture, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, bacteria, [SDV.IMM]Life Sciences [q-bio]/Immunology, Leukocyte Common Antigens, Panton–Valentine leukocidin, CRISPR-Cas Systems, Cellular Tropism
Abstract

International audience; The staphylococcal bi-component leukocidins Panton-Valentine leukocidin (PVL) and γ-haemolysin CB (HlgCB) target human phagocytes. Binding of the toxins' S-components to human complement C5a receptor 1 (C5aR1) contributes to cellular tropism and human specificity of PVL and HlgCB. To investigate the role of both leukocidins during infection, we developed a human C5aR1 knock-in (hC5aR1KI) mouse model. HlgCB, but unexpectedly not PVL, contributed to increased bacterial loads in tissues of hC5aR1KI mice. Compared to humans, murine hC5aR1KI neutrophils showed a reduced sensitivity to PVL, which was mediated by the toxin's F-component LukF-PV. By performing a genome-wide CRISPR-Cas9 screen, we identified CD45 as a receptor for LukF-PV. The human-specific interaction between LukF-PV and CD45 provides a molecular explanation for resistance of hC5aR1KI mouse neutrophils to PVL and probably contributes to the lack of a PVL-mediated phenotype during infection in these mice. This study demonstrates an unsuspected role of the F-component in driving the sensitivity of human phagocytes to PVL.

Published
2017
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