1. Cultivation-dependent characterization of bacterial diversity from British Columbia forest soils subjected to disturbance.
- Author
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Axelrood PE, Chow ML, Arnold CS, Lu K, McDermott JM, and Davies J
- Subjects
- Bacteria chemistry, Bacteria classification, Bacteria genetics, British Columbia, DNA, Bacterial genetics, DNA, Bacterial isolation & purification, Ecosystem, Fatty Acids analysis, Genes, Bacterial, Genetic Variation, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Soil analysis, Bacteria isolation & purification, Soil Microbiology, Trees microbiology
- Abstract
Bacteria from forest surface organic matter and mineral soil horizons were cultivated using four methods and characterized by fatty acid methyl ester (FAME) analysis. Soil samples from a British Columbia Ministry of Forests Long-Term Soil Productivity (LTSP) installation were collected during winter and summer from two disturbance treatments (whole-tree harvesting with no soil compaction (plot N) and whole-tree harvesting plus complete surface organic matter removal with heavy soil compaction (plot S)) and from an unlogged reference plot (REF). Seventy-five percent of 1795 bacterial isolates were affiliated with 42 genera representing beta- and gamma-Proteobacteria, Actinobacteria, the Bacillus/Clostridium group, and the Cytophaga-Flexibacter-Bacteroides group. Approximately half of the culture collection represented genetic diversity confined to four bacterial genera: Pseudomonas, Bacillus, Paenibacillus, and Arthrobacter. A significantly higher proportion of bacterial isolates belonging to Actinobacteria, and the member genus Arthrobacter, were isolated from plot S soil samples compared with soil samples from plots N and REF. Twenty-five percent of bacterial isolates were not conclusively identified to genus with FAME analysis. Sherlock Tracker cluster analysis and partial 16S rRNA gene sequence analysis enabled classification of a subset of these isolates.
- Published
- 2002
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