7 results on '"Alessio Mancini"'
Search Results
2. Synthesis of Bioactive Silver Nanoparticles Using New Bacterial Strains from an Antarctic Consortium
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KESAVA PRIYAN RAMASAMY, Joseph Amruthraj Nagoth, Maria Sindhura John, Cristina Miceli, Sandra Pucciarelli, Gabriele GIULI, and Alessio Mancini
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nanomaterials ,green synthesis ,capped nanoparticles ,antimicrobial activity ,nosocomial pathogens ,Silver ,Anti-Infective Agents ,Bacteria ,Plant Extracts ,Drug Discovery ,Pharmaceutical Science ,Antarctic Regions ,Metal Nanoparticles ,Microbial Sensitivity Tests ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Anti-Bacterial Agents - Abstract
In this study, we report on the synthesis of silver nanoparticles (AgNPs) achieved by using three bacterial strains Rhodococcus, Brevundimonas and Bacillus as reducing and capping agents, newly isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii. After incubation of these bacteria with a 1 mM solution of AgNO3 at 22 °C, AgNPs were synthesized within 24 h. Unlike Rhodococcus and Bacillus, the reduction of Ag+ from AgNO3 into Ag0 has never been reported for a Brevundimonas strain. The maximum absorbances of these AgNPs in the UV-Vis spectra were in the range of 404 nm and 406 nm. EDAX spectra showed strong signals from the Ag atom and medium signals from C, N and O due to capping protein emissions. TEM analysis showed that the NPs were spherical and rod-shaped, with sizes in the range of 20 to 50 nm, and they were clustered, even though not in contact with one another. Besides aggregation, all the AgNPs showed significant antimicrobial activity. This biosynthesis may play a dual role: detoxification of AgNO3 and pathogen protection against both the bacterium and ciliate. Biosynthetic AgNPs also represent a promising alternative to conventional antibiotics against common nosocomial pathogens.
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- 2022
3. Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents
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Kesava Priyan Ramasamy, Sandra Pucciarelli, Joseph Amruthraj Nagoth, Maria Sindhura John, Alessio Mancini, Cristina Miceli, Marco Zannotti, and Rita Giovannetti
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metal ,QH301-705.5 ,Microorganism ,Pharmaceutical Science ,Nanoparticle ,chemistry.chemical_element ,Antarctic Regions ,Metal Nanoparticles ,02 engineering and technology ,medicine.disease_cause ,Gram-Positive Bacteria ,Article ,antibiotics ,Absorbance ,03 medical and health sciences ,Anti-Infective Agents ,Microscopy, Electron, Transmission ,X-Ray Diffraction ,Drug Discovery ,Gram-Negative Bacteria ,Spectroscopy, Fourier Transform Infrared ,medicine ,Biology (General) ,Psychrophile ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Escherichia coli ,030304 developmental biology ,0303 health sciences ,biology ,Bacteria ,nanotechnology ,green synthesis ,Fungi ,Green Chemistry Technology ,021001 nanoscience & nanotechnology ,Antimicrobial ,biology.organism_classification ,Copper ,6. Clean water ,Dynamic Light Scattering ,chemistry ,13. Climate action ,Spectrophotometry, Ultraviolet ,0210 nano-technology ,Azo Compounds ,Nuclear chemistry ,biomaterials - Abstract
In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable strategy alternative to chemical and physical approaches. Herein, an original and efficient route for the microbial synthesis of copper NPs using bacterial strains newly isolated from an Antarctic consortium is described. UV-visible spectra of the NPs showed a maximum absorbance in the range of 380–385 nm. Transmission electron microscopy analysis showed that these NPs are all monodispersed, spherical in nature, and well segregated without any agglomeration and with an average size of 30 nm. X-ray powder diffraction showed a polycrystalline nature and face centered cubic lattice and revealed characteristic diffraction peaks indicating the formation of CuONPs. Fourier-transform infrared spectra confirmed the presence of capping proteins on the NP surface that act as stabilizers. All CuONPs manifested antimicrobial activity against various types of Gram-negative, Gram-positive bacteria, and fungi pathogen microorganisms including Escherichia coli, Staphylococcus aureus, and Candida albicans. The cost-effective and eco-friendly biosynthesis of these CuONPs make them particularly attractive in several application from nanotechnology to biomedical science.
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- 2021
4. Horizontal gene transfer and silver nanoparticles production in a new Marinomonas strain isolated from the Antarctic psychrophilic ciliate Euplotes focardii
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Andrea Telatin, Kesava Priyan Ramasamy, Maria Sindhura John, Alessio Mancini, Gabriele Giuli, Sandra Pucciarelli, Joseph Amruthraj Nagoth, Matteo Mozzicafreddo, Patrizia Ballarini, Antonino Natalello, Cristina Miceli, Pietro Liò, Mozzicafreddo, Matteo [0000-0001-9835-8446], Telatin, Andrea [0000-0001-7619-281X], Natalello, Antonino [0000-0002-1489-272X], Miceli, Cristina [0000-0002-7829-8471], Apollo - University of Cambridge Repository, Lio, Pietro [0000-0002-0540-5053], John, M, Nagoth, J, Ramasamy, K, Ballarini, P, Mozzicafreddo, M, Mancini, A, Telatin, A, Lio, P, Giuli, G, Natalello, A, Miceli, C, and Pucciarelli, S
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0301 basic medicine ,DNA, Bacterial ,Marinomonas ,Silver ,Gene Transfer, Horizontal ,030106 microbiology ,lcsh:Medicine ,Euplotes ,Metal Nanoparticles ,Biology ,DNA, Ribosomal ,Microbiology ,03 medical and health sciences ,Environmental biotechnology ,RNA, Ribosomal, 16S ,Humans ,Seawater ,lcsh:Science ,Psychrophile ,Gene ,Phylogeny ,631/61/168 ,Ciliate ,Whole genome sequencing ,704/158/2464 ,Multidisciplinary ,45 ,lcsh:R ,101/28 ,article ,Ecological genetics ,Fibroblasts ,16S ribosomal RNA ,biology.organism_classification ,Anti-Bacterial Agents ,030104 developmental biology ,Genes, Bacterial ,Horizontal gene transfer ,lcsh:Q ,nanoparticles ,Bacteria ,Genome, Bacterial - Abstract
Funder: Fondi di Ateneo per la Ricerca (FAR-UNICAM) 2014-2015, Funder: European Commission Marie Sklodowska-Curie Actions H2020 RISE Metable– 645693, We isolated a novel bacterial strain from a prokaryotic consortium associated to the psychrophilic marine ciliate Euplotes focardii, endemic of the Antarctic coastal seawater. The 16S rDNA sequencing and the phylogenetic analysis revealed the close evolutionary relationship to the Antarctic marine bacterium Marinomonas sp. BSw10506 and the sub antarctic Marinomonas polaris. We named this new strain Marinomonas sp. ef1. The optimal growth temperature in LB medium was 22 °C. Whole genome sequencing and analysis showed a reduced gene loss limited to regions encoding for transposases. Additionally, five genomic islands, e.g. DNA fragments that facilitate horizontal gene transfer phenomena, were identified. Two open reading frames predicted from the genomic islands coded for enzymes belonging to the Nitro-FMN-reductase superfamily. One of these, the putative NAD(P)H nitroreductase YfkO, has been reported to be involved in the bioreduction of silver (Ag) ions and the production of silver nanoparticles (AgNPs). After the Marinomonas sp. ef1 biomass incubation with 1 mM of AgNO3 at 22 °C, we obtained AgNPs within 24 h. The AgNPs were relatively small in size (50 nm) and had a strong antimicrobial activity against twelve common nosocomial pathogenic microorganisms including Staphylococcus aureus and two Candida strains. To our knowledge, this is the first report of AgNPs biosynthesis by a Marinomonas strain. This biosynthesis may play a dual role in detoxification from silver nitrate and protection from pathogens for the bacterium and potentially for the associated ciliate. Biosynthetic AgNPs also represent a promising alternative to conventional antibiotics against common pathogens.
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- 2020
5. Antibiotic activity of the antioxidant drink effective Microorganism-X (EM-X) extracts against common nosocomial pathogens: an in vitro study
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Alessio Mancini and Sandra Pucciarelli
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medicine.drug_class ,medicine.medical_treatment ,Antibiotics ,Plant Science ,Microbial Sensitivity Tests ,Effective microorganism ,01 natural sciences ,Biochemistry ,Antioxidants ,Analytical Chemistry ,Microbiology ,medicine ,Humans ,Cross Infection ,biology ,Bacteria ,010405 organic chemistry ,Organic Chemistry ,biology.organism_classification ,Antimicrobial ,Yeast ,3. Good health ,0104 chemical sciences ,Anti-Bacterial Agents ,010404 medicinal & biomolecular chemistry ,Beneficial organism ,Photosynthetic bacteria ,Fermented Foods ,Adjuvant - Abstract
EM-X is a mixed consortium of beneficial microorganisms of natural occurrence (lactic bacteria, yeast and photosynthetic bacteria). The aim of this study is to evaluate the antimicrobial activity in-vitro of EM-X to the principal pathogens isolated in clinical settings and to understand if it could be a suitable adjuvant to synthetic antibiotics. According the World Health Organization we performed antimicrobial assays to the main pathogens usually found in hospital wards. After antimicrobial testing, EM-X has been shown to be most effective at a concentration of 40 mg/ml four time concentrated than the commercial original solution (10 mg/ml). The EM-X antimicrobial action, although effective against bacteria, has proved to be ineffective against the candida genus. This active range of concentration (mg/ml) may prove a very weak action of EM, but further investigations will be done to separate the active substances form the inactive ones in this complex mixture.
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- 2018
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6. Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences
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Michele Castelli, Alessio Mancini, Patrizia Ballarini, Martina Schrallhammer, Cristina Miceli, Giulio Petroni, Sandra Pucciarelli, and Raghul Rajan Devaraj
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Evolution ,Microbial Consortia ,Antarctic Regions ,Euplotes ,Soil Science ,Genome ,Behavior and Systematics ,Proteobacteria ,Psychrophile ,Gene ,Gene transfer ,Ecology, Evolution, Behavior and Systematics ,Genetics ,Ciliate ,biology ,Ecology ,Bacteroidetes ,Catabolism ,Genomics ,biology.organism_classification ,Antifreeze proteins ,Adaptation, Physiological ,Genes and Genomes ,Transmembrane transporter activity ,Bacteria - Abstract
We report the characterization of the bacterial consortium associated to Euplotes focardii, a strictly psychrophilic marine ciliate that was maintained in laboratory cultures at 4 °C after its first isolation from Terra Nova Bay, in Antarctica. By Illumina genome analyser, we obtained 11,179 contigs of potential prokaryotic origin and classified them according to the NCBI’s prokaryotic attributes table. The majority of these sequences correspond to either Bacteroidetes (16 %) or Proteobacteria (78 %). The latter were dominated by gamma- (39 %, including sequences related to the pathogenic genus Francisella), and alpha-proteobacterial (30 %) sequences. Analysis of the Pfam domain family and Gene Ontology term variation revealed that the most frequent terms that appear unique to this consortium correspond to proteins involved in “transmembrane transporter activity” and “oxidoreductase activity”. Furthermore, we identified genes that encode for enzymes involved in the catabolism of complex substance for energy reserves. We also characterized members of the transposase and integrase superfamilies, whose role in bacterial evolution is well documented, as well as putative antifreeze proteins. Antibiotic treatments of E. focardii cultures delayed the cell division of the ciliate. To conclude, our results indicate that this consortium is largely represented by bacteria derived from the original Antarctic sample and may contribute to the survival of E. focardii in laboratory condition. Furthermore, our results suggest that these bacteria may have a more general role in E. focardii survival in its natural cold and oxidative environment. Electronic supplementary material The online version of this article (doi:10.1007/s00248-015-0568-9) contains supplementary material, which is available to authorized users.
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7. Differences between Community - And Hospital - Acquired urinary tract infections in a tertiary care hospital
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Alessio Mancini, Pucciarelli, S., Lombardi, F. E., Barocci, S., Pauri, P., and Lodolini, S.
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Community-Acquired Infections ,Tertiary Care Centers ,Cross Infection ,Bacteria ,Drug Resistance, Bacterial ,Urinary Tract Infections ,Humans ,Bacterial Infections ,Anti-Bacterial Agents ,Retrospective Studies - Abstract
The aim of this retrospective study was to highlight the differences in antibiotic resistance between Hospital-acquired and Community-acquired urinary tract infections (UTIs). Antimicrobial UTIs resistance data were collected from March 2011 to March 2018. Uropathogens were identified from 41,715 patients using routine laboratory methods. Differences in antibiotic resistance between Hospital and Community (non-hospitalized) patients were statistically validated. Odds ratio (OR) and p-values was used to determine whether a particular exposure (hospitalization) was a risk factor for a particular outcome (higher antibiotic resistance). We reported a general increase of unnecessary urine cultures in both community and hospital patients. The most representative microorganism isolated from Community (58.2%) and Hospital (47.6%) was E. coli. UTIs causative bacteria in hospitalized patients was more than twice as resistant to Trimetoprim/sulphamethoxazole (OR 2.26) and Imipenem (OR 2.56), for Gram-positive and Gram-negative, respectively, than in Community patients. Nitrofurantoin was the only agent without differences in resistance rate between community and hospital UTIs. Therefore, physicians could use it as a definitive therapy for uncomplicated cystitis and as a prophylactic agent for recurrent uncomplicated cystitis. With this work we provided a general protocol applicable by physicians to select the most suitable, if necessary, UTIs empiric treatment.
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