1. Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica.
- Author
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Ferhat M, Erol E, Beladjila KA, Çetintaş Y, Duru ME, Öztürk M, Kabouche A, and Kabouche Z
- Subjects
- Acetylcholinesterase metabolism, Anti-Bacterial Agents isolation & purification, Antioxidants isolation & purification, Bacteria growth & development, Benzothiazoles chemistry, Biphenyl Compounds chemistry, Butyrylcholinesterase metabolism, Chelating Agents isolation & purification, Chelating Agents pharmacology, Chlorides chemistry, Cholinesterase Inhibitors isolation & purification, Copper chemistry, Ferric Compounds chemistry, Linoleic Acid chemistry, Microbial Sensitivity Tests, Oxidation-Reduction, Phytotherapy, Picrates chemistry, Plant Components, Aerial, Plant Extracts isolation & purification, Plants, Medicinal, Solvents chemistry, Sulfonic Acids chemistry, beta Carotene chemistry, Anti-Bacterial Agents pharmacology, Antioxidants pharmacology, Bacteria drug effects, Cholinesterase Inhibitors pharmacology, Mentha chemistry, Plant Extracts pharmacology, Stachys chemistry
- Abstract
Context: Stachys guyoniana Noë ex. Batt. and Mentha aquatica L. are two Algerian Lamiaceae used in folk medicine., Objective: To investigate their antioxidant, anticholinesterase and antibacterial activities., Material and Methods: n-Butanol (BESG), ethyl acetate (EESG) and chloroform (CESG) extracts of S. guyoniana and methanol (MEMA) and chloroform (CEMA) aerial part extracts of M. aquatica and methanol (MERMA) and acetone (AERMA) roots extracts of M. aquatica were evaluated for their antioxidant activity by the β-carotene-linoleic acid, DPPH
• and ABTS• + scavenging, CUPRAC and metal chelating assays. The anticholinesterase activity was tested against AChE and BChE. The antibacterial activity was assessed by MICs determination against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella heidelberg, Klebsiella pneumoniae, Enterobacter aerogenes and Morganella morganii strains., Results: In the β-carotene test, the CESG (IC50 : 2.3 ± 1.27 μg/mL) exhibited the highest activity. The BESG was the best scavenger of DPPH• (IC50 : 2.91 ± 0.14 μg/mL). In the ABTS test, AERMA was the most active (IC50 : 4.21 ± 0.28 μg/mL). However, with the CUPRAC, the BESG exhibited the best activity (A0.50 : 0.15 ± 0.05 μg/mL) and was active in metal chelating assay with 48% inhibition at 100 μg/mL. The BESG was the best AChE inhibitor (IC50 : 5.78 ± 0.01 μg/mL) however, the AERMA showed the highest BChE inhibitory activity (IC50 : 19.23 ± 1.42 μg/mL). The tested extracts exhibited a good antibacterial activity., Conclusion: This study demonstrated good antioxidant, anticholinesterase and antibacterial potential of S. guyoniana and M. aquatica, which fits in well with their use in folk medicine.- Published
- 2017
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