1. Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis.
- Author
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Zhang, Ying, Cao, Rong, Ying, Haijian, Du, Juping, Chen, Shuaishuai, Wang, Na, and Shen, Bo
- Subjects
RHEUMATOID arthritis ,B cells ,ENZYME-linked immunosorbent assay - Abstract
Toll-like receptor (TLR) 10, mainly expressed on B cells, has emerged as a modulatory receptor in inflammation. Nonetheless, the clinical significance of TLR10 in rheumatoid arthritis (RA) remains unclear. In this study, we explored the expression of TLR10 in B cells and B cell subsets in RA subjects and healthy controls (HCs) and determined its relevance to disease activity and inflammatory biomarkers. TLR10 levels in B cells and B cell subsets (CD19
+ CD27+ , CD19+ CD27− , CD27+ IgD− , CD27+ IgD+ , CD27− IgD+ , D27− IgD− , CD19+ CD5+ , and CD19+ CD5− ) and inflammatory biomarker concentrations in peripheral blood (PB) obtained from RA subjects and HCs were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively. The correlations of TLR10 expression with disease activity and inflammatory biomarkers were then analysed. Similar levels of TLR10 in all CD19+ B cells were observed in the RA subjects and HCs. Compared to that in the HCs, TLR10 was elevated significantly in the CD19+ CD27− IgD− and CD19+ CD5+ subsets in the RA subjects. In addition, almost all subsets expressing TLR10 were increased with disease activity. The present study reveals that enhanced TLR10 in B cell subsets is positively correlated with disease activity in RA subjects. [ABSTRACT FROM AUTHOR]- Published
- 2018
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