1. Reproducible automated renewable column generation.
- Author
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Metzger, Shalina C., Ticknor, Brian W., Tevepaugh, Kayron N., Bostick, Debra A., McBay, Eddy H., Field, M. Paul, Kim, Hwan, and Hexel, Cole R.
- Subjects
PACKED towers (Chemical engineering) ,STANDARD deviations - Abstract
Preparation of chromatographic columns requires considerable time during chemical separation procedures. Automated systems capable of repeatedly generating renewable, uniformly packed columns would find widespread use in analytical laboratories. Here, a fully automated commercial off-the-shelf (COTS) system was utilized to pack and unpack 1 mL TEVA and UTEVA columns. To accurately determine the reproducibility of the resin load per column, studies were conducted over several weeks. Method optimization yielded an improvement in loading from 15–19% to 3–5% relative standard deviation. Availability of automated COTS equipment capable of reproducibly generating separation columns has the potential to reduce time and costs associated with column separation. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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