1. Idiopathic atrophoderma of Pasini and Pierini : a case study of collagen and elastin texture by multiphoton microscopy
- Author
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Vieira-Damiani, Gislaine, 1976, Cintra, Maria Letícia, 1951, Metze, Konradin, 1956, Adur, Javier Fernando, Pelegati, Vitor Bianchin, 1982, Carvalho, Hernandes Faustino de, 1965, César, Carlos Lenz, 1955, and UNIVERSIDADE ESTADUAL DE CAMPINAS
- Subjects
Idiopathic atrophoderma of Pasini and Pierini ,Fluorescência excitada por dois fótons ,Second harmonic generation ,Patologia ,Fibras elásticas ,Scleroderma ,Fibra de colágeno ,Atrophoderma ,Fibers elastic ,Two photon excited fluorescence ,Collagen fiber ,Atrofia ,Geração de segundo harmônico ,Pathology ,Artigo original ,Atrophy ,Morphometrics ,Morfometria - Abstract
Agradecimentos: This study was funded by the São Paulo Research Foundation (08/57906–3), the National Council for Scientific and Technological Development (573913/2008–0), and the Biologia das Doenças Neoplásicas da Medula Óssea (FAPESP grant 11/51959-0) project with fellowship support from CNPq (310518/2015-6) Abstract: Background: The diagnosis of idiopathic atrophoderma of Pasini and Pierini (IAPP) relies on typical clinical features, particularly distinctive pigmented ovular/round depressed plaques. Histologic examination often reveals no obvious changes, but patterns of collagen distribution, using multiphoton imaging and second harmonic generation can help track hidden details of tissue organization contributing to atrophy. Objective: To identify histologic features that distinguish IAPP from unaffected skin. Methods: Eleven patients were included for conventional analyses. Masson trichromee and Unna-Tanzer orcein-stained sections were evaluated using automated morphometry. Hematoxylin-eosinestained sections were analyzed by multiphoton imaging using 2-photon excited fluorescence and second harmonic generation. Results: No abnormalities were found under light microscopy or by automated quantification. Multiphoton imaging revealed no difference in optical density of either collagen or elastic fibers in lesioned and unaffected skin; however, horizontal collagen fiber organization in lesion specimens increased toward the lower dermis, whereas elastic fibers featured greater disorganization within the upper dermis. Limitations: The low number of patients evaluated. Conclusion: The atrophic appearance of IAPP lesions reflects changes in organization, but not in collagen and elastic tissue content. Minute organizational differences that are imperceptible to the experienced pathologist and undetectable by automated analyses were revealed by multiphoton analyses, particularly second harmonic generation, in association with texture analyses FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ Fechado
- Published
- 2017