1. Clinical validation of the influence of P-glycoprotein on technetium-99m-sestamibi uptake in malignant tumors.
- Author
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Kostakoglu L, Elahi N, Kïratlï P, Ruacan S, Sayek I, Baltalï E, Sungur A, Hayran M, and Bekdik CF
- Subjects
- Adult, Aged, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Breast Neoplasms drug therapy, Breast Neoplasms metabolism, Carcinoma, Ductal, Breast diagnostic imaging, Carcinoma, Ductal, Breast drug therapy, Carcinoma, Ductal, Breast metabolism, Carcinoma, Medullary diagnostic imaging, Carcinoma, Medullary drug therapy, Carcinoma, Medullary metabolism, Carcinoma, Small Cell diagnostic imaging, Carcinoma, Small Cell drug therapy, Carcinoma, Small Cell metabolism, Carcinoma, Squamous Cell diagnostic imaging, Carcinoma, Squamous Cell drug therapy, Carcinoma, Squamous Cell metabolism, Drug Resistance, Multiple, Female, Humans, Immunohistochemistry, Lung Neoplasms drug therapy, Lung Neoplasms metabolism, Male, Middle Aged, Prospective Studies, Radionuclide Imaging, ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, Breast Neoplasms diagnostic imaging, Lung Neoplasms diagnostic imaging, Neoplasm Proteins metabolism, Radiopharmaceuticals, Technetium Tc 99m Sestamibi
- Abstract
Unlabelled: We prospectively studied 48 patients with either breast cancer (30 patients) or lung cancer (18 patients) to ascertain the relationship between the degree of accumulation of 99mTc-sestamibi and the expression of p-glycoprotein in tumor tissues., Methods: During initial presentation (37 patients) or post-therapy evaluation (11 patients), the patients underwent contemporaneous 99mTc-sestamibi imaging and biopsy (30 patients) or surgery (18 patients). The interval between surgery/biopsy and imaging varied between 3 and 15 days. All patients had radiologically detectable tumors. Immunohistochemical studies were performed on paraffin sections using a monoclonal antibody, JSB-1, developed against the internal epitope of p-glycoprotein. Tumor-to-background ratios were correlated with the level of p-glycoprotein expression determined by immunohistochemical studies., Results: Our results showed an inverse correlation between the tumor-to-background ratios of 99mTc-sestamibi and the density of p-glycoprotein expression in tumor tissues. The values for the tumor-to-background ratios were significantly lower for those tumors expressing p-glycoprotein at high levels than those with scattered and no expression (p < 0.01 and p < 0.001, respectively)., Conclusion: Although our results warrant further studies at the molecular level using PCR techniques after the extraction of mRNA, our data strongly suggest that 99mTc-sestamibi imaging is useful to noninvasively determine the presence of multidrug resistance in patients with malignant tumors.
- Published
- 1997