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The purpose of this study is to prepare nanoencapsulation synthesized with a new formulation of Fe@Au-CS-CU-FA nanoparticle (NPs) by pulsed laser ablation in liquid (PLAL) method as drug delivery to treat breast cancer (T-47D) and (MCF12A) as a normal cell line. The method synthesized Fe@Au NPs using the PLAL working at wavelength 532 nm with different laser fluence (1.9, 2.2, and 2.5) J/cm2. These Fe@Au NPs were characterized by atomic force microscope (AFM), field emission scanning electron microscopy (FESEM), and high-resolution transmission electron microscopy (HRTEM). The obtained mean sizes of Fe@Au NPs were 63.65, 32.47, and 31.18 nm at 1.9, 2.2, and 2.5 J/cm2, respectively. Results of the MTT assay of CU loaded Fe@Au-CS-FA NPs on human breast cancer cell line (T-47D) confirmed that cytotoxicity of CU can enhance when they are loaded on Fe@Au-CS-FA NPs in comparison with free CU. While results of flow cytometry showed that this combination can increase the therapeutic effects of CU by apoptosis induction in the T-47D cell line. Conclusion of Fe@Au-CS-CU-FA NPs causes a decrease in T-47D cell viability and caused induces 85% apoptosis. The in vivo study of Fe@Au-CS-CU-FA nanoformulation confirmed that the mean tumor size decreases in time. [ABSTRACT FROM AUTHOR]

Among the considerable anticancer agents, curcumin (CU) drug has been confirmed to be very extraordinary towards any type of cancer cells. Chitosan coated core–shell nanocarrier was prepared and used as a nano-carrier for encapsulation of CU drug (Fe@Au-CU-CS-FA NP's) through a reverse micro-emulsion method. Each shape and size and polydispersity (PDI) properties in addition to the contents of Fe@Au core–shell and Fe@Au-CU-CS-FA NP's were studied utilizing zeta potential particles analyzer, Atomic Force Microscope, Scanning Electron Microscopy, Energy Dispersive X-rays spectroscopy, and High-Resolution Transmission Electron Microscopy. On the other hand, those nanosystems characterized by UV–Visible spectrophotometer. Fe@Au NP's possess a nearly uniform size of around ~ 63.65 nm and a spherical form as core–shell at temperature 38 °C and the Fe@Au-CU-CS-FA NP's possess a nearly uniform size of around 206.5 nm and a spherical form. EDX results for Fe@Au core–shell structure show percentage ratio of (Fe, Au) elements, and the results of Fe@Au-CU-CS-FA NP's show percentage ratio of (Fe, Au, C, O, N) elements. Fe@Au-CU-CS-FA NP's use as drug delivery to treat different types of cancers. Finally, the results showed that synthesized nanosystems are noticeably potent for cancer therapy purposes due to their suitable characteristics such as small size, spherical shape, negligible aggregation, good polydispersity (PDI), correct content and appropriate magnetic responsive properties. This Fe@Au-CU-CS-FA also exhibited a controlled release of CU at 37 °C in (7.4) and (5.4) pH. Afterward, the ideal dose and therapeutic effects of (Fe@Au-CU-CS-FA) for lung cancer and normal cell lines were evaluated through MTT assay. Then the flow cytometry assay shows significant cell apoptosis induction by Fe@Au-CU-CS-FA. The results confirmed that Fe@Au-CU-CS-FA retained considerable antitumor effects; no adverse consequences were detected for normal cells. [ABSTRACT FROM AUTHOR]

In the present work, we investigated novel, green, and facile approach for the synthesis of stable silver nanoparticles by use of inexpensive and available Iraqi Zahdi palm dates extract as reducing and stabilizing agents. The formation and color change of dark brown suspension of silver nanoparticles were monitored and examined by ultraviolet-visible spectrophotometric analysis at maximum wavelength of 401 nm. The produced nanoparticles were characterized by dynamic light scattering (DLS), Fourier transform-infrared spectrometry (FTIR), atomic force microscope (AFM), X-ray diffraction (XRD) and scanning electron microscopy (SEM)-energy dispersive spectroscopy (EDS). The results demonstrated that particle size distribution was around 90 ± 40 nm which was in line with grain size of the nanoparticles measured by AFM, while the crystallite size was about 20 nm according to the results observed from XRD technique. Additionally, the zeta potential of synthesized silver nanoparticles was found to be nearly about -25 mV, whereby the suspension showed high stability upon storing the sample in a cool place for a long period of time. The synthesized silver nanoparticles exhibited efficient antibacterial activity against pathogenic methicillin-resistant Staphylococcus aureus (MRSA) bacteria. They also showed enhanced anticancer activity against breast cancer cells (MCF7) with high apoptotic effect as compared with normal MCF10A cells, which confirmed the biocompatible nature of green synthesized silver nanoparticles. It is considered that nanosilver particles synthesized with palm dates extract may basically establish a significant step to synthesize such nanoparticles for topical applications as well as an alternative anticancer drug of existing chemotherapeutics of cancer. [ABSTRACT FROM AUTHOR]