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This paper experimentally analyses an indoor environment assessment of a large group of respondents regarding their subjective perception of overall comfort, indoor air quality and humidity. The questionnaire survey was applied as a testing method together with measurements of the physical parameters conducted with a microclimate meter. Two types of environment were analysed: educational rooms and the climate chamber. The comparative analysis of the sensations experienced within them indicates that they generate quite similar responses; however, some discrepancies have been identified. The overall comfort of the climate chamber was typically assessed as being higher than that of the educational rooms at the same air temperature. The most favourable air temperature in the climate chamber was ca. 20.7 °C, while in the educational rooms it was ca. 22.3 °C. The most preferable conditions in the climate chamber occurred at a thermal sensation vote of −0.4 ("pleasantly slightly cool"), while in the educational rooms it occurred at +0.2 ("neutral/pleasantly slightly warm"). Quite strong correlations between overall comfort and indoor air quality as well as between humidity assessment and humidity preference votes were observed, which did not seem to depend on the type of environment. These findings are important because results from the simulated conditions are often used in the analyses of actual living/working environments. [ABSTRACT FROM AUTHOR]