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The interest in whole-body vibration (WBV) for the enhancement of neuromuscular performance has received considerable attention. However, scientific evidence supporting the optimal prescription of WBV settings is lacking. This study investigated the acute eff ect of WBV combining high frequency/high peak-to-peak displacement (HH) or low frequency/low peakto- peak displacement (LL) vs. sham intervention (SHAM) on lower limb muscle power and stiffness. A total of 223 volunteers were randomly assigned to either the HH, LL or SHAM group. Countermovement jump (CMJ) height, maximal and average power, maximal and average lower limbs stiff ness obtained during a hopping test were recorded before and after the respective intervention. After the intervention, the HH group showed an increase of 4.64 % in CMJ height (p < 0.001) whereas the values of both the LL and SHAM groups did not change. In addition, maximal and average power of the lower limbs were signifi cantly increased in all groups (p < 0.001; 10.89 % and 12.82 %, respectively) while no eff ect on lower limbs stiff ness was observed. Our data show that high frequency combined with high peak-to-peak displacement is the most optimal WBV setting for CMJ height enhancement. Further investigation should be undertaken to ascertain the eff ectiveness of WBV on lower limbs stiff ness. [ABSTRACT FROM AUTHOR]