1. Zika virus induces astrocyte differentiation in neural stem cells.
- Author
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Lossia OV, Conway MJ, Tree MO, Williams RJ, Goldthorpe SC, Srinageshwar B, Dunbar GL, and Rossignol J
- Subjects
- Animals, Astrocytes virology, Cell Differentiation, Cell Survival, Chromatography, Liquid, Embryo, Mammalian, Extracellular Space chemistry, Gene Expression Regulation, Gene Ontology, Mass Spectrometry, Mice, Molecular Sequence Annotation, Neural Stem Cells virology, Signal Transduction, Virus Replication, Astrocytes metabolism, Host-Pathogen Interactions, Neural Stem Cells metabolism, Zika Virus physiology
- Abstract
Zika virus (ZIKV) is a rapidly emerging flavivirus that has been associated with a number of congenital neurological manifestations. Here, we show that ZIKV replicated efficiently in mouse neural stem cells (mNSCs). ZIKV infection caused a cytopathic effect without affecting cell viability, yet led to a significant decrease in the number of proteins secreted into mNSC supernatants. A gene expression array of neural stem cell progenitor and differentiation markers suggested that infection reduced the number of neuronal and oligodendrocyte progenitors while increasing the number of astrocyte progenitors. Infection in astrocytes increased transcription of key genes involved in the antiviral response. These data provide molecular and cellular evidence that ZIKV significantly alters neural development in the vertebrate host and that astrocyte differentiation may be a protective response that limits neuropathogenesis.
- Published
- 2018
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